FastQCFastQC Report
Wed 25 May 2016
SRR1294710_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294710_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3316562
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC61820.1863978421027558No Hit
GTATCAACGCAGAGTACTTTTTTTT56760.1711410792260178No Hit
CCCATGTACTCTGCGTTGATACCAC55560.16752287459121826No Hit
GGTATCAACGCAGAGTACTTTTTTT39570.11931029783251453No Hit
TATCAACGCAGAGTACTTTTTTTTT34040.10263640480714668No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGA751.4852472E-512.6621256
TATACCG600.005887144711.0810295
ACCGCGT600.005896401611.078698
GTCTACG750.002647529410.1339771
GTCCTAG3300.09.7885011
TGGCGTC1606.6842404E-89.49616312
CTAGTAC2650.09.3192393
TTAGACT3400.09.2189494
CGCCCTA1356.802642E-69.15342316
GTGTAAG4800.09.1047451
TATTCCG1151.1138182E-49.0850675
TAGGACT4400.09.066574
TAGTACC2201.6916601E-109.066574
TGGACCG1502.7114565E-68.8648235
CCTACAC3350.08.7896143
CTAATAC3350.08.7896143
TACCGCT1304.393052E-58.7655577
ACCGCTC1856.625851E-88.7260728
GTCTTAG3050.08.7218661
CTTGCAC3300.08.6349593
Produced by FastQC (version 0.11.5)