##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294710_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3316562
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.058384254538282	33.0	33.0	33.0	27.0	33.0
2	31.110430319107557	33.0	33.0	33.0	27.0	33.0
3	31.053856674471938	33.0	33.0	33.0	27.0	33.0
4	34.544285317144684	37.0	37.0	37.0	27.0	37.0
5	34.74463435328512	37.0	37.0	37.0	27.0	37.0
6	34.91505088703302	37.0	37.0	37.0	33.0	37.0
7	34.88217105544838	37.0	37.0	37.0	33.0	37.0
8	34.81587981771485	37.0	37.0	37.0	33.0	37.0
9	34.535068845388686	37.0	37.0	37.0	27.0	37.0
10	34.61873289267621	37.0	37.0	37.0	27.0	37.0
11	34.831724538844746	37.0	37.0	37.0	33.0	37.0
12	34.76394621900631	37.0	37.0	37.0	27.0	37.0
13	34.68863178194769	37.0	37.0	37.0	27.0	37.0
14	35.84031958395471	40.0	37.0	40.0	27.0	40.0
15	36.14930038998216	40.0	37.0	40.0	27.0	40.0
16	36.16933740421557	40.0	37.0	40.0	27.0	40.0
17	36.124641119327784	40.0	37.0	40.0	27.0	40.0
18	36.1257606521452	40.0	37.0	40.0	27.0	40.0
19	36.230883366570566	40.0	37.0	40.0	27.0	40.0
20	36.1154361052198	40.0	37.0	40.0	27.0	40.0
21	36.06817391021184	40.0	37.0	40.0	27.0	40.0
22	35.92876991293996	40.0	37.0	40.0	27.0	40.0
23	35.552147072782	37.0	37.0	40.0	27.0	40.0
24	35.860162421206056	37.0	37.0	40.0	27.0	40.0
25	34.690510836221364	37.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.5559937586805859
1101	2	0.6425891201937475
1101	3	0.49151553710210294
1101	4	0.6200078211493718
1101	5	0.5266765640847026
1101	6	0.4856833806311087
1101	7	0.4725210683282981
1101	8	0.5861741582188387
1101	9	0.8207100784855967
1101	10	0.7296914297277226
1101	11	0.5532628377173765
1101	12	0.5515615714940267
1101	13	0.5766778427691648
1101	14	1.2147059181260786
1101	15	1.0438646006689325
1101	16	0.9700545981233475
1101	17	0.9521730038907279
1101	18	0.9173245234760543
1101	19	0.9029168307906161
1101	20	1.0283399515636944
1101	21	0.9679561272506945
1101	22	1.0136962190705887
1101	23	1.4588963730760511
1101	24	1.2170438584852334
1101	25	1.0153279290510184
1102	1	0.3101152506544409
1102	2	0.4677618479665675
1102	3	0.38971489882012733
1102	4	0.29058461491138843
1102	5	0.2773529426432262
1102	6	0.297225249514824
1102	7	0.2683680070954253
1102	8	0.3752253316182319
1102	9	0.621030040520381
1102	10	0.7451652108218028
1102	11	0.4972370092193259
1102	12	0.550345641867608
1102	13	0.5206250018232197
1102	14	1.2300702536485986
1102	15	0.9886945905618418
1102	16	0.6815730671661768
1102	17	0.9464599532657871
1102	18	0.8635171641029373
1102	19	0.8490648064580668
1102	20	0.9193503484824674
1102	21	0.9648063034063341
1102	22	0.9732679855615274
1102	23	1.3261231306709433
1102	24	1.0553871661717977
1102	25	0.8321264285054326
1103	1	0.2936477746766357
1103	2	0.43497677648514355
1103	3	0.2684260993366756
1103	4	0.10845968916979132
1103	5	0.3036608329587267
1103	6	0.21203281348817882
1103	7	0.15246904298036412
1103	8	0.36281281040314184
1103	9	0.532720766354636
1103	10	0.576458658146791
1103	11	0.33719747203655714
1103	12	0.2990485570306234
1103	13	0.2741588960183563
1103	14	0.8912306378864514
1103	15	0.5484548908959397
1103	16	0.28960725489420014
1103	17	0.4226931923586861
1103	18	0.5997504064523369
1103	19	0.5994657835387329
1103	20	0.7169515037588852
1103	21	0.6682401456684133
1103	22	0.634860588939766
1103	23	1.1191979933218903
1103	24	0.7860795936094149
1103	25	0.5323982079660468
1104	1	0.3480210022703325
1104	2	0.4466714413813335
1104	3	0.3526913130805127
1104	4	0.34790719699282846
1104	5	0.3468013953937472
1104	6	0.30350136308724984
1104	7	0.3066177282473248
1104	8	0.5282773127397604
1104	9	0.7210474604019268
1104	10	0.6521526308073433
1104	11	0.46961110020050967
1104	12	0.4184770573239831
1104	13	0.4678953697597237
1104	14	1.0357395719722362
1104	15	0.812032616863263
1104	16	0.715316490835896
1104	17	0.7352680612456481
1104	18	0.6789620909524388
1104	19	0.6854868375382566
1104	20	0.9139400696473601
1104	21	0.9133276690860512
1104	22	0.9842554295969066
1104	23	1.3087150302960282
1104	24	1.0435599684717403
1104	25	0.9766007023303729
1105	1	0.1601162828629903
1105	2	0.2488017960565969
1105	3	0.15657489207636388
1105	4	0.11770150261235557
1105	5	0.16098648674949345
1105	6	0.12043816256521467
1105	7	0.18393185000380896
1105	8	0.15989796566467618
1105	9	-1.5831364794107614
1105	10	-0.19798843321844828
1105	11	0.049058695394734286
1105	12	0.1059794563720402
1105	13	0.1943926156525535
1105	14	-1.7908675503216358
1105	15	-0.37593951579449225
1105	16	-0.206521803728819
1105	17	0.048890096022255136
1105	18	0.07435903744406858
1105	19	0.13858112972311432
1105	20	0.257036808861244
1105	21	0.4500635722796602
1105	22	0.48450157192763044
1105	23	0.8406737090922789
1105	24	0.6109014873570473
1105	25	0.386267426574193
1106	1	0.1475009745694642
1106	2	0.2509949163512992
1106	3	0.20971169107037468
1106	4	-0.8951577978897944
1106	5	-0.04772101278993546
1106	6	0.029094969201288734
1106	7	0.14129687061611662
1106	8	0.24091477780753223
1106	9	0.4685313090822305
1106	10	0.45679690862551325
1106	11	0.37958477374276356
1106	12	0.18055501870002644
1106	13	0.331444391850809
1106	14	0.8544018144276464
1106	15	0.56363646997778
1106	16	0.40932845834505827
1106	17	0.3100979036123803
1106	18	0.4593558244853071
1106	19	0.44115583151599225
1106	20	0.5361290165632724
1106	21	0.6931286988132399
1106	22	0.7199679406179555
1106	23	1.022523522435634
1106	24	0.6169648081315415
1106	25	0.4817822886658192
1107	1	0.3673585706217679
1107	2	0.3652685743156425
1107	3	0.32867363407363825
1107	4	0.534075383093473
1107	5	0.473099149032997
1107	6	0.2734209577816955
1107	7	0.28360800202253955
1107	8	0.3913186976876659
1107	9	0.594782035145144
1107	10	0.512025256980408
1107	11	0.26584241901377936
1107	12	0.33158875650376984
1107	13	0.4346208048073805
1107	14	0.8479099379295221
1107	15	0.6696353287527188
1107	16	0.63122748156227
1107	17	0.5942175966406111
1107	18	0.5852588516485113
1107	19	0.45347180945874754
1107	20	0.5865926827043353
1107	21	0.5982084928132352
1107	22	0.58038272029372
1107	23	0.8895994886570691
1107	24	0.7787841658041827
1107	25	0.7398588028993558
1108	1	0.3753561088040378
1108	2	0.3453732504954061
1108	3	0.4423657550937463
1108	4	0.6349665688665524
1108	5	0.44104405372203104
1108	6	0.3416317162612046
1108	7	0.3391260167347383
1108	8	0.43830260171259283
1108	9	0.12885828946011202
1108	10	0.4136297680112264
1108	11	0.32182080139607194
1108	12	0.2748383559091181
1108	13	0.27183864613626696
1108	14	-1.2022177501717977
1108	15	-0.1823853594521765
1108	16	0.2045803816754983
1108	17	0.34075196137477093
1108	18	0.4968456421704204
1108	19	0.4582622364698423
1108	20	0.5222962956535611
1108	21	0.543646615687372
1108	22	0.6474219736765008
1108	23	0.9561816515007564
1108	24	0.7631810527697525
1108	25	0.6734400350378351
1109	1	0.3882641441842303
1109	2	0.3129073032869414
1109	3	0.3893559811683893
1109	4	0.5586309600946251
1109	5	0.5610231340092469
1109	6	0.31312507511425025
1109	7	0.2946012732819696
1109	8	0.37593333142277174
1109	9	0.4731200606269468
1109	10	0.44968734686111844
1109	11	0.35594054370734796
1109	12	0.21448415597975412
1109	13	0.277013978008668
1109	14	0.6374038514475728
1109	15	0.5065942619156729
1109	16	0.4397240417626449
1109	17	0.47746573919318536
1109	18	0.5375658801097742
1109	19	0.4909834843671774
1109	20	0.634628968652514
1109	21	0.5864476171229995
1109	22	0.7479151604549585
1109	23	1.0852637438034591
1109	24	0.7827153653746848
1109	25	0.718237205261623
1110	1	0.3347144310279653
1110	2	0.22841631512165605
1110	3	0.34959196869702325
1110	4	0.5256328326684852
1110	5	0.4012277795811343
1110	6	0.2961980237230222
1110	7	0.24992671914262132
1110	8	0.2004389625782892
1110	9	0.331976996217648
1110	10	0.27639512137191247
1110	11	0.07339931612595763
1110	12	0.11608670772669427
1110	13	0.062065161304794
1110	14	0.48422558662146997
1110	15	0.2638608531186648
1110	16	0.26856678994877115
1110	17	0.324976693691859
1110	18	0.3911610934231575
1110	19	0.3105993088428747
1110	20	0.3754569488543993
1110	21	0.5555372985751745
1110	22	0.5944949387599721
1110	23	0.8140011816130936
1110	24	0.5485831801171983
1110	25	0.5231619985834399
1111	1	0.14269567627240676
1111	2	0.061772659805427566
1111	3	0.17035782250757237
1111	4	0.3691591122077682
1111	5	0.25559236910913796
1111	6	0.18665245389129126
1111	7	0.12022594749052473
1111	8	0.18227784936561875
1111	9	0.20315505617180918
1111	10	0.01130205596860634
1111	11	-0.020884931327856293
1111	12	0.03273974070923913
1111	13	0.024238685065348875
1111	14	-1.9071789093491631
1111	15	-0.656525861642443
1111	16	-0.11781012269840829
1111	17	-0.03306783637308541
1111	18	0.07819973027403648
1111	19	0.11919457482378704
1111	20	0.09513437300278582
1111	21	0.23329255071753607
1111	22	0.04211663273584776
1111	23	-2.0517446487157756
1111	24	-0.5210939627011939
1111	25	-0.1311100261643645
1112	1	-0.14674006553999774
1112	2	-0.272019660456408
1112	3	-0.04804926083979666
1112	4	0.04720993621728553
1112	5	-0.6250399421553325
1112	6	-0.8209609130107225
1112	7	-0.9290938297974805
1112	8	-0.7138119742037787
1112	9	-0.6040288550002586
1112	10	-0.7720851943342453
1112	11	-0.7726766156901874
1112	12	-0.7865025671757522
1112	13	-0.7877646768721931
1112	14	-0.5966267410680857
1112	15	-0.8402726898463158
1112	16	-0.7645702689165716
1112	17	-0.8440611242921676
1112	18	-0.9513334398490798
1112	19	-0.9061115992422089
1112	20	-0.9543549235044253
1112	21	-1.8655901046286942
1112	22	-1.7241281229503471
1112	23	-1.4235546200298899
1112	24	-1.6112484730443128
1112	25	-2.104238360592447
1113	1	0.08430704644269227
1113	2	-0.02843383392079346
1113	3	-0.01969753494810078
1113	4	0.3150462955910811
1113	5	-0.016475911289070666
1113	6	-0.035791111170773604
1113	7	0.011816687752386201
1113	8	0.12163500901330337
1113	9	0.2629917365981669
1113	10	0.010058914156545029
1113	11	-0.18419156449044038
1113	12	-0.06627109314759139
1113	13	-0.10310709808083374
1113	14	0.016854687991383344
1113	15	-0.15085964179670697
1113	16	0.01813102784088727
1113	17	0.05373103282131808
1113	18	0.0835056486086927
1113	19	-0.13790167519572805
1113	20	-0.8043991493685283
1113	21	-0.4490850371381754
1113	22	-0.06572014436981277
1113	23	-0.33542695796505484
1113	24	-0.016387691315976838
1113	25	-1.1639738232097017
1114	1	-1.0169597200109024
1114	2	-0.7743687689525878
1114	3	-0.6235727059911689
1114	4	-1.7134776393835551
1114	5	-0.9255265480132238
1114	6	-0.6506962992445082
1114	7	-0.6188435285169973
1114	8	-0.5710266005788398
1114	9	-0.6182248397306154
1114	10	-0.7389948696830828
1114	11	-0.679236012074071
1114	12	-0.6833450481922583
1114	13	-0.6754596186733508
1114	14	-0.7260393414659774
1114	15	-1.4142608578091043
1114	16	-0.9634628944900143
1114	17	-0.9975549500676948
1114	18	-0.9293758700501442
1114	19	-0.8552039815248094
1114	20	-2.083284936154044
1114	21	-6.931614348236835
1114	22	-7.87576639745177
1114	23	-8.215061545533754
1114	24	-6.157754222309645
1114	25	-7.642037560764059
1115	1	-1.1437144845586538
1115	2	-1.082379583963494
1115	3	-0.8626826267711927
1115	4	-0.7183740551669615
1115	5	-0.7255299682112053
1115	6	-0.967105963660579
1115	7	-1.170669766939426
1115	8	-0.9137419704815102
1115	9	-2.8341468960053717
1115	10	-1.8454274788852985
1115	11	-1.2460163835578726
1115	12	-1.1003033957135457
1115	13	-1.4609569086334915
1115	14	-1.1602444684146036
1115	15	-2.1571792696329837
1115	16	-1.4535620320899625
1115	17	-3.056566837626818
1115	18	-2.893048158176242
1115	19	-2.1454239828857027
1115	20	-1.977892792851776
1115	21	-1.3738255766462046
1115	22	-0.9566689172826344
1115	23	-0.32726642142834805
1115	24	-1.3402486574822703
1115	25	-1.1087015880342506
1116	1	-1.6814673690551416
1116	2	-0.9169015798003848
1116	3	-0.7055859121732588
1116	4	-0.3369950539313038
1116	5	-0.3244529318960474
1116	6	-0.5014286301651154
1116	7	-0.9020374014246784
1116	8	-1.8190009870020702
1116	9	-0.8637087444915821
1116	10	-2.474829487951091
1116	11	-1.1388072203709854
1116	12	-0.9185903970561142
1116	13	-0.9194965396752366
1116	14	-1.268269497000361
1116	15	-1.2400965564043531
1116	16	-0.8508044055731858
1116	17	-0.7814273012198782
1116	18	-1.4487759283223411
1116	19	-0.9889918707290661
1116	20	-1.2682099516223673
1116	21	-0.7070658759177633
1116	22	-0.40642641192160767
1116	23	-0.1681000477418806
1116	24	-0.23231071524004676
1116	25	-0.21256328728413365
1201	1	0.30750447241575074
1201	2	0.34088936440687334
1201	3	0.3735005597790355
1201	4	0.5095934701802278
1201	5	0.4288083580369815
1201	6	0.4344554186342364
1201	7	0.35078731742568436
1201	8	0.43904508225314487
1201	9	0.7193543305524841
1201	10	0.6214068443626601
1201	11	0.4877800502801577
1201	12	0.527575542756388
1201	13	0.4262843432194643
1201	14	1.1276857238914602
1201	15	0.8166360545077893
1201	16	0.759970462240247
1201	17	0.6688853760033737
1201	18	0.6131651926686885
1201	19	0.6788512908037632
1201	20	0.8211786654286684
1201	21	0.8317634990793721
1201	22	0.9976548245406391
1201	23	0.5936379209491278
1201	24	0.743529217867966
1201	25	0.40032748281441854
1202	1	-0.2485644155623099
1202	2	0.10496642718238292
1202	3	0.025779248117718367
1202	4	-0.0530792479318265
1202	5	-0.030174279833744322
1202	6	0.03816915167833912
1202	7	-0.037170165851300396
1202	8	0.17582899769923444
1202	9	0.2067241388837786
1202	10	0.4411032298579869
1202	11	0.22943069190293386
1202	12	0.2787906649976364
1202	13	0.3696620444586429
1202	14	0.4649733144322781
1202	15	0.48187202361657455
1202	16	0.394805775550779
1202	17	0.2472949032227234
1202	18	0.23106728626574835
1202	19	0.34101425861489076
1202	20	0.5027947674791733
1202	21	0.4967725794875548
1202	22	0.4100811322895197
1202	23	0.7282451305178199
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2211	7	0.24539439129866025
2211	8	0.3289975106094971
2211	9	0.43094511878114616
2211	10	0.4201927748643044
2211	11	0.21960632031842664
2211	12	0.29241244722331317
2211	13	0.16105083933533848
2211	14	0.5595916181093941
2211	15	0.3882778195997929
2211	16	0.19194417104613848
2211	17	0.3649484348759984
2211	18	0.27118209415061045
2211	19	0.4004311823293989
2211	20	0.4277578396846309
2211	21	0.4667272081316014
2211	22	0.4824468960299626
2211	23	0.6215113829852754
2211	24	0.5420108521415798
2211	25	0.6450585211745832
2212	1	-0.21395471389946508
2212	2	-0.167915725837414
2212	3	-0.17802194296169205
2212	4	-0.502326861711623
2212	5	-0.2414316434971866
2212	6	-0.5933345312815419
2212	7	-0.5246970644161522
2212	8	-0.28029007929802674
2212	9	-0.1815871856537754
2212	10	-0.6086014177756525
2212	11	-0.5361617727598613
2212	12	-0.5994291044966502
2212	13	-0.5871946882086263
2212	14	-0.5290003755802104
2212	15	-0.6567978947304027
2212	16	-0.4989737419576272
2212	17	-0.4814394363006613
2212	18	-0.3752806783396778
2212	19	-0.4892865798338448
2212	20	-0.4527758624468561
2212	21	-0.35679543697455784
2212	22	-0.3768601543805232
2212	23	0.04332773119162425
2212	24	-0.20687038516584977
2212	25	-0.9537740216298758
2213	1	-0.20430227141184432
2213	2	-1.3321001339499574
2213	3	-0.37686820173963653
2213	4	0.029639415552161097
2213	5	-0.023613482553855647
2213	6	-0.0499357596503458
2213	7	-0.018121523706867038
2213	8	0.03017101708955039
2213	9	0.08583544766764106
2213	10	-0.41733196756404567
2213	11	-0.1894961527348542
2213	12	-0.12345478548781585
2213	13	-0.46536563552370325
2213	14	-0.21112015545494955
2213	15	-0.2484397797639204
2213	16	-0.17707293923276524
2213	17	-0.03831437161038309
2213	18	-0.014530008677468231
2213	19	-0.06649539238264168
2213	20	0.08196571853870438
2213	21	-0.6052461318895297
2213	22	-1.0815741660710145
2213	23	-0.3590271255590878
2213	24	-0.18348022730851454
2213	25	-0.8551468907645869
2214	1	-0.2721380096419175
2214	2	-0.45347702504778553
2214	3	-0.47491689274636073
2214	4	-0.5513516716929843
2214	5	-0.3480363395325412
2214	6	-0.3012541476372661
2214	7	-0.5064981891005402
2214	8	-0.18553650599146465
2214	9	-0.07357630820004601
2214	10	-2.0124053758998457
2214	11	-0.9007930996527733
2214	12	-0.6365583562860238
2214	13	-0.39215744498910965
2214	14	-0.8234062875610562
2214	15	-0.6314916268312771
2214	16	-0.8152340794498585
2214	17	-0.5535562374688041
2214	18	-0.5733988527700546
2214	19	-1.4511946321047233
2214	20	-2.4820512275998965
2214	21	-1.936020934456188
2214	22	-2.064401167552539
2214	23	-2.562816313587156
2214	24	-1.5814429790235423
2214	25	-1.9980108832910872
2215	1	-0.19463632795632435
2215	2	-0.34867620016937906
2215	3	-0.5113450700471702
2215	4	-0.1736788765610342
2215	5	-0.12725128685006126
2215	6	-0.16321534952323447
2215	7	-0.22244583830557474
2215	8	-0.13466257196314046
2215	9	-0.2557454423722092
2215	10	-0.36407264704335773
2215	11	-0.42804562838092153
2215	12	-0.33898471085664283
2215	13	-0.2211258354997554
2215	14	-0.12652209447565355
2215	15	-0.5954726338587051
2215	16	-0.3806425043071826
2215	17	-0.2167240681408913
2215	18	-0.2785104794947486
2215	19	-2.001784810750884
2215	20	-2.239999749421507
2215	21	-3.2852200413803914
2215	22	-2.5485574806157487
2215	23	-3.3309363983379114
2215	24	-2.082149015732341
2215	25	-1.3121602913687624
2216	1	-1.478977925632087
2216	2	-1.1963366598502674
2216	3	-1.005750316290559
2216	4	-0.9443943020126184
2216	5	-1.7415532652283758
2216	6	-1.5290137150766014
2216	7	-1.2053003427374236
2216	8	-5.370540535980123
2216	9	-4.055064286383896
2216	10	-2.0897556425392168
2216	11	-1.1889763999201861
2216	12	-1.2580490296797677
2216	13	-1.9633936382377115
2216	14	-3.530801953779836
2216	15	-2.139464095330581
2216	16	-3.048535723544994
2216	17	-2.8226662076761286
2216	18	-2.777245717647517
2216	19	-1.6082874377925762
2216	20	-1.0147427726272582
2216	21	-0.8291255922269514
2216	22	-0.7613942470538788
2216	23	-1.1312309846270452
2216	24	-0.61799631861264
2216	25	-0.42968120713184277
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	4.0
7	52.0
8	255.0
9	735.0
10	1641.0
11	3077.0
12	5504.0
13	8253.0
14	11081.0
15	14043.0
16	15942.0
17	17091.0
18	18017.0
19	18848.0
20	18896.0
21	20596.0
22	21990.0
23	23322.0
24	24936.0
25	26866.0
26	29415.0
27	33341.0
28	37949.0
29	45097.0
30	54318.0
31	67318.0
32	89377.0
33	126496.0
34	183388.0
35	286808.0
36	496026.0
37	1615880.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.20929714906691	20.041043446998064	13.228126825698967	23.521532578236055
2	15.601665932370576	19.907535988801307	40.20070558767661	24.290092491151505
3	19.14552648403479	24.676805510319788	28.587709140037877	27.589958865607546
4	12.681355115398684	16.444813972754023	37.017116418607216	33.85671449324008
5	13.94721375080555	37.55413438808125	34.37107307995379	14.127578781159409
6	32.84208336223039	37.18900484565956	16.44870747232667	13.520204319783376
7	27.82408409672426	32.19641303253188	21.614310240544274	18.365192630199587
8	24.875126411329347	37.341831310450544	19.857267513207987	17.925774765012118
9	24.325972891172114	17.66617680137058	21.12702386027924	36.880826447178066
10	15.243983150015936	30.18623809421868	32.44234012279587	22.12743863296951
11	33.472022423429785	25.28768764712285	22.143770101952914	19.09651982749445
12	21.697116766103257	26.879146478581912	30.32607381414067	21.097662941174164
13	27.33940522553403	23.1398372493279	25.957429615219006	23.56332790991906
14	20.841353749816076	22.756695540166007	27.259073925324493	29.142876784693428
15	23.41363588531159	30.289501067239883	23.160145683746624	23.1367173637019
16	22.581330885760607	30.506081291536844	24.940094672579356	21.97249315012319
17	21.127263385529048	29.67717093844942	27.427082592088265	21.768483083933265
18	21.444588739759162	29.179125831712398	28.236053599863233	21.14023182866521
19	21.925169807531866	28.209654071144435	28.478125907381436	21.387050213942263
20	22.767655637022482	27.835758094186513	28.03261946838082	21.363966800410186
21	22.909766413348912	27.890381328518043	27.64891269710986	21.550939561023185
22	22.13795762985994	27.652961709482586	28.283281513274467	21.925799147383007
23	22.176225371492713	27.91181036784908	28.09817154220208	21.813792718456124
24	22.102465049271437	27.59942180462327	28.08559317792172	22.212519968183575
25	22.47199397348806	27.457464048369225	27.55938655523381	22.511155422908903
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3167.0
1	3167.0
2	3328.5
3	3490.0
4	3490.0
5	3490.0
6	4812.0
7	6134.0
8	6134.0
9	6134.0
10	9163.5
11	12193.0
12	12193.0
13	12193.0
14	19888.0
15	27583.0
16	27583.0
17	27583.0
18	43121.0
19	58659.0
20	58659.0
21	58659.0
22	83353.0
23	108047.0
24	108047.0
25	108047.0
26	140438.0
27	172829.0
28	172829.0
29	172829.0
30	208205.5
31	243582.0
32	243582.0
33	243582.0
34	274607.0
35	305632.0
36	305632.0
37	305632.0
38	329385.5
39	353139.0
40	353139.0
41	353139.0
42	359319.5
43	365500.0
44	365500.0
45	365500.0
46	374934.0
47	384368.0
48	384368.0
49	384368.0
50	371429.5
51	358491.0
52	358491.0
53	358491.0
54	333579.5
55	308668.0
56	308668.0
57	308668.0
58	274844.0
59	241020.0
60	241020.0
61	241020.0
62	206667.0
63	172314.0
64	172314.0
65	172314.0
66	137950.0
67	103586.0
68	103586.0
69	103586.0
70	78049.0
71	52512.0
72	52512.0
73	52512.0
74	38035.0
75	23558.0
76	23558.0
77	23558.0
78	15889.0
79	8220.0
80	8220.0
81	8220.0
82	5513.0
83	2806.0
84	2806.0
85	2806.0
86	1790.0
87	774.0
88	774.0
89	774.0
90	500.5
91	227.0
92	227.0
93	227.0
94	135.0
95	43.0
96	43.0
97	43.0
98	31.5
99	20.0
100	20.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.031689442259785885
2	0.013899936138688196
3	0.018151326584577646
4	0.01878451239566756
5	0.015347217992608006
6	0.005789127415679249
7	0.0
8	2.412136423199687E-4
9	1.2060682115998435E-4
10	3.0151705289996087E-5
11	9.347028639898787E-4
12	0.0012965233274698318
13	0.002351833012619695
14	7.839443375398982E-4
15	0.0018995574332697535
16	0.0023819847179096906
17	0.002110619370299726
18	0.0018392540226897613
19	3.919721687699491E-4
20	4.522755793499413E-4
21	0.08062565994544954
22	0.0762838143836901
23	0.07739942747941995
24	0.001085461390439859
25	0.2166400025086219
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3316562.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.90449813547865
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.55763647573117	59.246122796984835
2	10.96698607802902	15.552172878428577
3	2.7653227111232197	5.882214570444992
4	1.0044624893385015	2.8488363480984003
5	0.5023683391261219	1.7810087482445809
6	0.2839198249315632	1.207871561849127
7	0.16560457005683718	0.8219476250175227
8	0.12424449445674478	0.7047594820441397
9	0.0871325500177299	0.5560280757244468
>10	0.49743717430172035	6.344110437904048
>50	0.030020002409506252	1.451006473862671
>100	0.013028610112592636	1.567201594065262
>500	8.96983441041571E-4	0.3942604239517582
>1k	8.115564346035384E-4	1.1150622298655701
>5k	1.2814048967424292E-4	0.5273967535140159
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	6182	0.1863978421027558	No Hit
GTATCAACGCAGAGTACTTTTTTTT	5676	0.1711410792260178	No Hit
CCCATGTACTCTGCGTTGATACCAC	5556	0.16752287459121826	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3957	0.11931029783251453	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3404	0.10263640480714668	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.0151705289996087E-5	0.0
5	0.0	0.0	0.0	3.0151705289996087E-5	0.0
6	0.0	0.0	0.0	3.0151705289996087E-5	0.0
7	0.0	0.0	0.0	3.0151705289996087E-5	0.0
8	0.0	3.0151705289996087E-5	0.0	3.0151705289996087E-5	0.0
9	0.0	3.0151705289996087E-5	0.0	9.045511586998826E-5	0.0
10	3.0151705289996087E-5	3.0151705289996087E-5	0.0	9.045511586998826E-5	0.0
11	3.0151705289996087E-5	3.0151705289996087E-5	0.0	9.045511586998826E-5	0.0
12	3.0151705289996087E-5	3.0151705289996087E-5	0.0	9.045511586998826E-5	0.0
13	3.0151705289996087E-5	3.0151705289996087E-5	0.0	1.2060682115998435E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGACCGA	75	1.4852472E-5	12.662125	6
TATACCG	60	0.0058871447	11.081029	5
ACCGCGT	60	0.0058964016	11.07869	8
GTCTACG	75	0.0026475294	10.133977	1
GTCCTAG	330	0.0	9.788501	1
TGGCGTC	160	6.6842404E-8	9.496163	12
CTAGTAC	265	0.0	9.319239	3
TTAGACT	340	0.0	9.218949	4
CGCCCTA	135	6.802642E-6	9.153423	16
GTGTAAG	480	0.0	9.104745	1
TATTCCG	115	1.1138182E-4	9.085067	5
TAGGACT	440	0.0	9.06657	4
TAGTACC	220	1.6916601E-10	9.06657	4
TGGACCG	150	2.7114565E-6	8.864823	5
CCTACAC	335	0.0	8.789614	3
CTAATAC	335	0.0	8.789614	3
TACCGCT	130	4.393052E-5	8.765557	7
ACCGCTC	185	6.625851E-8	8.726072	8
GTCTTAG	305	0.0	8.721866	1
CTTGCAC	330	0.0	8.634959	3
>>END_MODULE