FastQCFastQC Report
Wed 25 May 2016
SRR1294710_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294710_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3316562
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT153690.46340155860194987No Hit
GGTATCAACGCAGAGTACTTTTTTT121190.36540851640946254No Hit
TATCAACGCAGAGTACTTTTTTTTT103710.3127033355625494No Hit
GTACATGGGAAGCAGTGGTATCAAC64830.1954735053950446No Hit
CCCATGTACTCTGCGTTGATACCAC61950.18678981427152574No Hit
ACGCAGAGTACTTTTTTTTTTTTTT57960.17475928386081732No Hit
GAGTACATGGGAAGCAGTGGTATCA34170.10302837697591663No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAC402.720471E-416.6582891
ACGTCGC400.005298516714.2427976
AACCGTG907.5172957E-611.6041937
TCGCCTA600.00590417411.07672912
CCTAGTA2700.010.2193472
TAGACAG5700.010.0150285
ACTGCGT1901.0186341E-109.99404058
ACCGTGA1252.591949E-69.8741138
CTTAGAC2500.09.5142773
CTGTCGC1901.0641088E-99.4943399
TACGAGG1106.885723E-59.49433910
CGTCATT1201.7137309E-59.49433912
GTAGGAC2357.2759576E-129.3118453
CTAGGAC4300.09.2930143
TAGGACC2401.0913936E-119.1178484
CGCGATC950.00183451578.99463717
GTATAGC2451.6370905E-118.9362251
CTTCTAG3950.08.9165531
TAGGACT4200.08.8346854
CTAGGGC2800.08.8346853
Produced by FastQC (version 0.11.5)