FastQCFastQC Report
Wed 25 May 2016
SRR1294709_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294709_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2638399
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC70860.2685719635278819No Hit
CCCATGTACTCTGCGTTGATACCAC63800.241813311784912No Hit
GTATCAACGCAGAGTACTTTTTTTT43340.16426628421250916No Hit
GGTATCAACGCAGAGTACTTTTTTT32930.12481053851218105No Hit
GAGTACATGGGAAGCAGTGGTATCA31570.1196558973832237No Hit
TATCAACGCAGAGTACTTTTTTTTT26480.10036389492264058No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT500.00149361313.307569516
GTCCTAA2050.010.6593451
TCTAGAC2850.010.3313743
CTAGACA2950.010.302744
TGGACGG1151.0300213E-59.9106015
CTAGACT2950.09.6588184
GCGCGAT900.00110827299.50540616
TAGAGCG800.0045264039.4976595
TATACTG6150.09.4204435
GGACCGC1356.8786685E-69.1452016
CTAAGAC2501.8189894E-129.1182713
GGAACGA1054.4796342E-49.05053615
GTGTACG950.00182307669.0006831
AATGCCG950.0018284318.9977825
TTACACT4150.08.925684
TAAACTG4950.08.8261075
AAACGGC1304.3403725E-58.77438817
GTCTAGT1856.570008E-88.7303921
CTATACT4600.08.671944
GTTCTAC2002.536217E-88.5506491
Produced by FastQC (version 0.11.5)