##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294709_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2638399
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.976608920788706	33.0	33.0	33.0	27.0	33.0
2	31.047857810740528	33.0	33.0	33.0	27.0	33.0
3	30.890611313906653	33.0	33.0	33.0	27.0	33.0
4	34.28586730058645	37.0	37.0	37.0	27.0	37.0
5	34.611323382096494	37.0	37.0	37.0	27.0	37.0
6	34.79137385967778	37.0	37.0	37.0	27.0	37.0
7	34.74672481304003	37.0	37.0	37.0	27.0	37.0
8	34.659258133436225	37.0	37.0	37.0	27.0	37.0
9	34.34334799247574	37.0	37.0	37.0	27.0	37.0
10	34.445742285378365	37.0	37.0	37.0	27.0	37.0
11	34.686357901136255	37.0	37.0	37.0	27.0	37.0
12	34.615661619034874	37.0	37.0	37.0	27.0	37.0
13	34.5416038286855	37.0	37.0	37.0	27.0	37.0
14	35.689561358990815	40.0	37.0	40.0	27.0	40.0
15	36.00495110860791	40.0	37.0	40.0	27.0	40.0
16	36.021434589688674	40.0	37.0	40.0	27.0	40.0
17	35.968795470283304	40.0	37.0	40.0	27.0	40.0
18	35.97263795203076	40.0	37.0	40.0	27.0	40.0
19	36.05488100927873	40.0	37.0	40.0	27.0	40.0
20	35.95130342302283	40.0	37.0	40.0	27.0	40.0
21	35.9075609867954	40.0	37.0	40.0	27.0	40.0
22	35.75906108211836	40.0	37.0	40.0	27.0	40.0
23	35.37580858694989	37.0	33.0	40.0	27.0	40.0
24	35.69640528214269	37.0	37.0	40.0	27.0	40.0
25	34.534837983186016	37.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.47537976710323804
1101	2	0.5847291413907278
1101	3	0.39514758222308544
1101	4	0.420797882978178
1101	5	0.5029304430071377
1101	6	0.4080956683892296
1101	7	0.34982979567231354
1101	8	0.5276414886522645
1101	9	0.7555661901720043
1101	10	0.7173812620765787
1101	11	0.5596680972778287
1101	12	0.5697146578614678
1101	13	0.5498104902871859
1101	14	1.200695802373275
1101	15	0.9211939387137491
1101	16	0.8133471658697289
1101	17	0.9234376804357254
1101	18	0.8153549729809839
1101	19	0.9477701024104164
1101	20	0.9899792317153171
1101	21	0.8798674864273082
1101	22	1.0925418485532177
1101	23	1.3182818658602642
1101	24	1.0249706162850813
1101	25	0.8871218776245158
1102	1	0.2450848307127167
1102	2	0.47095781898995526
1102	3	0.262544363158451
1102	4	0.12962163875593546
1102	5	0.1578067362264619
1102	6	0.15701952129393248
1102	7	0.1849134375860615
1102	8	0.18629879528725013
1102	9	0.4585203237042208
1102	10	0.4919686893397639
1102	11	0.15177907999443363
1102	12	0.3287148898387784
1102	13	0.2834493146174921
1102	14	1.0021934110557282
1102	15	0.7036493535412163
1102	16	0.49280519532000966
1102	17	0.6695569384552016
1102	18	0.7076204807728246
1102	19	0.6165205902131845
1102	20	0.709971502751543
1102	21	0.7533143766304917
1102	22	0.8218248652122284
1102	23	1.1592091138722012
1102	24	0.8649116897113274
1102	25	0.6186566460374436
1103	1	0.22509962512145165
1103	2	0.43502186007420107
1103	3	0.14126634730972754
1103	4	0.020656354601257476
1103	5	0.21685258188846745
1103	6	0.14307217748547885
1103	7	0.13145588223456883
1103	8	0.26817819534846876
1103	9	0.39855195312978964
1103	10	0.5729170619548043
1103	11	0.31804293912445303
1103	12	0.30803991619663407
1103	13	0.3020454187565207
1103	14	0.9655719353559746
1103	15	0.6196623879254659
1103	16	0.3831065676289569
1103	17	0.3887926456959576
1103	18	0.48999812931208453
1103	19	0.5610011704260884
1103	20	0.6255997974833747
1103	21	0.7502162658934921
1103	22	0.4682087376061972
1103	23	1.067344550732379
1103	24	0.7799394929967036
1103	25	0.5594907020861442
1104	1	0.20461921168181973
1104	2	0.5784385380637964
1104	3	0.23578930178312874
1104	4	0.12256705935087808
1104	5	0.126020178088595
1104	6	0.14209511405285014
1104	7	0.20629326654319868
1104	8	0.41796869483182775
1104	9	0.6070278763698056
1104	10	0.50363772161284
1104	11	0.4858779082260227
1104	12	0.35926181821696446
1104	13	0.4516179518589709
1104	14	0.8371298966389276
1104	15	0.6623781371188997
1104	16	0.6817962809323959
1104	17	0.6600322878492761
1104	18	0.5606239107258375
1104	19	0.5848453495453398
1104	20	0.7524225257088517
1104	21	0.754296733472529
1104	22	0.7686977433055802
1104	23	1.1901642944444717
1104	24	0.9827767951482684
1104	25	0.6678903667167901
1105	1	0.1367960917029727
1105	2	0.18221392762067268
1105	3	0.21329877245518247
1105	4	0.0762651242904866
1105	5	0.16996383490393185
1105	6	0.05921025042431438
1105	7	0.07178687414602081
1105	8	0.18177972791551866
1105	9	-1.7461692048097603
1105	10	-0.44418596060798876
1105	11	-0.0787340581793643
1105	12	0.053786333111638385
1105	13	0.12181092180746589
1105	14	-1.562198747203979
1105	15	-0.284860813436822
1105	16	-0.09464460308198852
1105	17	0.027534308815020836
1105	18	0.06729520608234196
1105	19	0.011620228181158154
1105	20	0.2221905453193287
1105	21	0.4742122925715151
1105	22	0.5095649884931035
1105	23	0.6923782530170399
1105	24	0.45388053173659415
1105	25	0.11746572691940571
1106	1	0.08055230001857083
1106	2	0.14386959669971588
1106	3	0.09959968064306324
1106	4	-1.0228107235880444
1106	5	-0.32918651276173705
1106	6	-0.04957185543633358
1106	7	0.05841061474485798
1106	8	0.24580165213859573
1106	9	0.44947787905571346
1106	10	0.44174422244863365
1106	11	0.3198433506470906
1106	12	0.13159958697853114
1106	13	0.21588286731619633
1106	14	0.6312097954382807
1106	15	0.3279957212587945
1106	16	0.2084666499334844
1106	17	0.11847371565480813
1106	18	0.2425701457729943
1106	19	0.21609376301867655
1106	20	0.4589331308167033
1106	21	0.5776339613667432
1106	22	0.5101326059189546
1106	23	0.882930970485468
1106	24	0.3668736493753073
1106	25	0.3246655992054883
1107	1	0.23773162152047433
1107	2	0.25386455400469643
1107	3	0.2576767500840269
1107	4	0.3674829052526576
1107	5	0.39094429178255297
1107	6	0.2520659314406828
1107	7	0.2636961728535212
1107	8	0.35357519187454756
1107	9	0.37422450404981333
1107	10	0.365415001921626
1107	11	0.16776041812784825
1107	12	0.26634360441227756
1107	13	0.2276554578033796
1107	14	0.6391017492693365
1107	15	0.38572747045384403
1107	16	0.5364576608077982
1107	17	0.5276510575888551
1107	18	0.5183286524092594
1107	19	0.4711741194376913
1107	20	0.5802764962998666
1107	21	0.5140172781835162
1107	22	0.41613217273723535
1107	23	0.7487195064159593
1107	24	0.5729050241948599
1107	25	0.5673209456722148
1108	1	0.48883138718654706
1108	2	0.3507470751883055
1108	3	0.5117592391915089
1108	4	0.7293151649267315
1108	5	0.5397351108821056
1108	6	0.47101194553635395
1108	7	0.29189415383950745
1108	8	0.3778414950117721
1108	9	0.216569910481077
1108	10	0.5085747499458293
1108	11	0.3198968485894724
1108	12	0.2898629614641237
1108	13	0.3785753476753442
1108	14	-1.1643554870158965
1108	15	-0.06325904866263699
1108	16	0.27982654518226724
1108	17	0.464030767656304
1108	18	0.6378587727838436
1108	19	0.5831349152820522
1108	20	0.6962683347074616
1108	21	0.6954583160244354
1108	22	0.7197876751295809
1108	23	1.033877846654569
1108	24	0.895895160785706
1108	25	0.7510140840539705
1109	1	0.3358355606577845
1109	2	0.239552736592767
1109	3	0.3969416017391403
1109	4	0.5300845352769414
1109	5	0.491845435687047
1109	6	0.2893868321268087
1109	7	0.29794605004709496
1109	8	0.3703582796243623
1109	9	0.5528529807042659
1109	10	0.4358663485667691
1109	11	0.3292860701463596
1109	12	0.3612082708453599
1109	13	0.21341040483375195
1109	14	0.7582197261940493
1109	15	0.46854425382329623
1109	16	0.5284638160949839
1109	17	0.4336391871808942
1109	18	0.44522440515546435
1109	19	0.5643884082960255
1109	20	0.5655363441431405
1109	21	0.6189016112521628
1109	22	0.6841687311992573
1109	23	1.056778953531719
1109	24	0.7328205826692127
1109	25	0.483203523357183
1110	1	0.43152249341747506
1110	2	0.25895450707715284
1110	3	0.42498400055786334
1110	4	0.6779201818503324
1110	5	0.5330639505826298
1110	6	0.35551827648516365
1110	7	0.25316147425726854
1110	8	0.3093144643608454
1110	9	0.4510830978438207
1110	10	0.5192457652894049
1110	11	0.25845317482721697
1110	12	0.28396261864342875
1110	13	0.1479436153798943
1110	14	0.6683356627018071
1110	15	0.5632782013790347
1110	16	0.3420479943063697
1110	17	0.3557717314680673
1110	18	0.5362088557710933
1110	19	0.3673638876172163
1110	20	0.4855860128038998
1110	21	0.6097326160686478
1110	22	0.7609753857686172
1110	23	0.9867421316958414
1110	24	0.7173405740187278
1110	25	0.6414608236334516
1111	1	0.17920525574223234
1111	2	0.06722405275619181
1111	3	0.33801661817616235
1111	4	0.5754229964891096
1111	5	0.48838524862315325
1111	6	0.23884508209676625
1111	7	0.1878059995695338
1111	8	0.23334720142656806
1111	9	0.2905156011540413
1111	10	0.06304306950505634
1111	11	0.09516013076184748
1111	12	0.14334741640312387
1111	13	0.1817300111936433
1111	14	-1.6167632005145194
1111	15	-0.42925353902076324
1111	16	-0.0828810502526025
1111	17	0.17776236938790646
1111	18	0.20037431784484028
1111	19	0.2426421958802436
1111	20	0.30019434808802004
1111	21	0.2960313187792565
1111	22	0.1638112878804634
1111	23	-1.8124629640498782
1111	24	-0.3893090895094531
1111	25	0.023165205659637422
1112	1	-0.13947119376663863
1112	2	-0.23431383980032194
1112	3	-0.004178919035805251
1112	4	0.13223228796433517
1112	5	-0.6957441488140219
1112	6	-0.904508935189348
1112	7	-0.9886019845602476
1112	8	-0.8739115310786048
1112	9	-0.7416899260679131
1112	10	-1.1087769505625005
1112	11	-0.9280308622538982
1112	12	-0.9472151973248728
1112	13	-0.9607882044351967
1112	14	-0.5851361200374896
1112	15	-0.9219928369626942
1112	16	-0.8934562362932894
1112	17	-0.9104873327172172
1112	18	-1.0204751503211682
1112	19	-1.0140056469669432
1112	20	-1.0706777152265374
1112	21	-2.1483332979840455
1112	22	-2.049259759363636
1112	23	-1.5162893676572224
1112	24	-1.758598562358621
1112	25	-2.1270064737015275
1113	1	0.1054358105538924
1113	2	-0.1669005515212696
1113	3	-0.05690147131363332
1113	4	0.3118342542832693
1113	5	0.07051406954182937
1113	6	-0.04060957003858334
1113	7	-0.0059304075640014275
1113	8	0.05912249316281759
1113	9	0.2475243924220507
1113	10	-0.00682455281631178
1113	11	-0.13182741782878082
1113	12	-0.1041590848019851
1113	13	-0.085273066602511
1113	14	-0.1408575547354758
1113	15	-0.09150723146881035
1113	16	-0.06853748637691837
1113	17	0.016347770944896922
1113	18	0.03710891745364364
1113	19	-0.07500698814909157
1113	20	-0.5036069528678695
1113	21	-0.5203986796425326
1113	22	-0.012139834715021891
1113	23	-0.16166733972247727
1113	24	0.09216276463039463
1113	25	-1.1005110038064672
1114	1	-0.8555330909279313
1114	2	-0.7286098271685972
1114	3	-0.5765320065997344
1114	4	-1.5139887071271403
1114	5	-0.8310898049428062
1114	6	-0.5676016908272814
1114	7	-0.5381428831975299
1114	8	-0.41214587872560315
1114	9	-0.4281455777344121
1114	10	-0.45817861705754126
1114	11	-0.47064018844756106
1114	12	-0.5816462977951318
1114	13	-0.7333455277455343
1114	14	-0.615575183985591
1114	15	-1.360738846642441
1114	16	-0.7843317039759867
1114	17	-0.7369532390776996
1114	18	-0.7276201834451186
1114	19	-0.8436798995327592
1114	20	-2.0067047292656
1114	21	-6.742582088015972
1114	22	-7.751338587496683
1114	23	-7.7124908402141195
1114	24	-5.773146926756382
1114	25	-7.130889956350071
1115	1	-1.03426533344442
1115	2	-0.8878677742985346
1115	3	-0.6758630393329881
1115	4	-0.42390107875715444
1115	5	-0.5420570192047762
1115	6	-0.7179254587378452
1115	7	-0.8679496502858299
1115	8	-0.5867046752405898
1115	9	-2.481176815159838
1115	10	-1.3704652866983693
1115	11	-1.0480162359641412
1115	12	-0.9351183013291333
1115	13	-1.235427658293652
1115	14	-1.0109312302775137
1115	15	-1.9003002018095998
1115	16	-1.234048721418553
1115	17	-2.973826806669855
1115	18	-2.788244603921328
1115	19	-2.042359022680472
1115	20	-1.6333720082592862
1115	21	-1.174154543797215
1115	22	-0.7903790273224303
1115	23	-0.2598882317010265
1115	24	-1.2243748611149243
1115	25	-0.9641767090924205
1116	1	-1.517562814601991
1116	2	-0.8270810634411454
1116	3	-0.5512588660358162
1116	4	-0.2501681304657595
1116	5	-0.3035835425371758
1116	6	-0.3146943852652271
1116	7	-0.8661050660684424
1116	8	-1.8851207272577284
1116	9	-0.9708613598633704
1116	10	-2.584739180370679
1116	11	-1.0993327325010966
1116	12	-0.6285912081278866
1116	13	-0.6080355495014373
1116	14	-1.0793228437094413
1116	15	-1.225499813436521
1116	16	-0.9006510129877796
1116	17	-0.7034719591200016
1116	18	-1.4029779965003897
1116	19	-0.9072721367500876
1116	20	-1.2082638299061443
1116	21	-0.6814683765980263
1116	22	-0.19809177937332123
1116	23	-0.150364634321825
1116	24	-0.11704086457437768
1116	25	-0.13377012120952259
1201	1	0.38498354907282817
1201	2	0.5241169996839687
1201	3	0.3451653496662743
1201	4	0.47082166733834896
1201	5	0.356005087584073
1201	6	0.4076806225351959
1201	7	0.35956742031761024
1201	8	0.5055309376510735
1201	9	0.6799193108878185
1201	10	0.7688451941669001
1201	11	0.48286316383760663
1201	12	0.5559337594413236
1201	13	0.550984800911742
1201	14	1.1988774387966146
1201	15	0.9023475651743667
1201	16	0.7252454033447577
1201	17	0.7913685112532391
1201	18	0.6657561367552631
1201	19	0.7621780283359669
1201	20	0.8815510824731092
1201	21	0.8804072551456557
1201	22	0.9335564869297599
1201	23	0.7203450073330941
1201	24	0.8667225293543979
1201	25	0.7885648449413196
1202	1	-0.41204476564612946
1202	2	-0.10089531141060348
1202	3	-0.21461286696320414
1202	4	-0.3136163238743208
1202	5	-0.18271736417906226
1202	6	-0.26259392685212646
1202	7	-0.09241019398658068
1202	8	-0.1786529525696352
1202	9	-0.15546039254922306
1202	10	0.15776484970114524
1202	11	0.025687738858621856
1202	12	-0.05568155199343039
1202	13	0.09578088127217654
1202	14	0.22882181394401613
1202	15	0.13681981450317693
1202	16	0.19783990863041367
1202	17	0.08009156193448774
1202	18	0.14073586670441074
1202	19	0.09282228588246966
1202	20	0.17128583894236016
1202	21	0.2692364074981768
1202	22	0.061972316575094055
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2211	1	0.3914730072573285
2211	2	0.26787651750364816
2211	3	0.3664077282283884
2211	4	0.5343362116063801
2211	5	0.38163831709768203
2211	6	0.35735367659957973
2211	7	0.3537490259887832
2211	8	0.38413330041898774
2211	9	0.5565011370522512
2211	10	0.4192476992034315
2211	11	0.2810910434754419
2211	12	0.3365581929775985
2211	13	0.22458435272235278
2211	14	0.6435888911123158
2211	15	0.37162792752634033
2211	16	0.44341599880627314
2211	17	0.5276422522325461
2211	18	0.46416758307053385
2211	19	0.4685548646024884
2211	20	0.5055191566797816
2211	21	0.574606731764419
2211	22	0.6840670410067347
2211	23	0.7192693148960672
2211	24	0.7396608620275629
2211	25	0.8759642631078108
2212	1	-0.2767071441946811
2212	2	-0.37585285671499946
2212	3	-0.28752347559682434
2212	4	-0.7026721236897728
2212	5	-0.2377793596660709
2212	6	-0.6660957607398927
2212	7	-0.5502631495312755
2212	8	-0.24653258284545387
2212	9	-0.2396988444843089
2212	10	-0.6151360660796001
2212	11	-0.5378010290634592
2212	12	-0.6163243044534781
2212	13	-0.5759433221691737
2212	14	-0.4314225622627461
2212	15	-0.5862226169295823
2212	16	-0.44370540351822285
2212	17	-0.5709446040381678
2212	18	-0.49847172318710165
2212	19	-0.4177539736068354
2212	20	-0.555383464180288
2212	21	-0.32703901685491843
2212	22	-0.5756675512919358
2212	23	-0.04259383646880366
2212	24	-0.24492699333982415
2212	25	-0.9202314045398339
2213	1	-0.08602216784578687
2213	2	-1.4106751322169444
2213	3	-0.2769024406367997
2213	4	0.07248493274009604
2213	5	0.05823350300873642
2213	6	0.057710116797444755
2213	7	0.08005581630417424
2213	8	0.06139090152244364
2213	9	0.08131285216007456
2213	10	-0.32184790891414394
2213	11	-0.1263117234939557
2213	12	-0.1423155090072612
2213	13	-0.4704741387080915
2213	14	-0.12692775102662068
2213	15	-0.12838693536834
2213	16	-0.0719661468075401
2213	17	0.03635900996942354
2213	18	-0.031575741971785476
2213	19	0.06294483200601775
2213	20	0.061861539272015875
2213	21	-0.4295775294808166
2213	22	-0.9351229993437826
2213	23	-0.048993183148390074
2213	24	0.20284425752898017
2213	25	-0.5791897373366481
2214	1	-0.17089065947427784
2214	2	-0.3534150484023897
2214	3	-0.35189384242075405
2214	4	-0.40171489345026856
2214	5	-0.15459885447226895
2214	6	-0.1537341383117692
2214	7	-0.3263114228966799
2214	8	-0.014170030877309614
2214	9	0.018803268959331376
2214	10	-1.8880084763516862
2214	11	-0.880225151859058
2214	12	-0.47783622370445045
2214	13	-0.3403582901154394
2214	14	-0.7126891669414093
2214	15	-0.5050122886199588
2214	16	-0.6482907796697503
2214	17	-0.46036277077804755
2214	18	-0.4461824648900503
2214	19	-1.2557395707761074
2214	20	-2.4090971025763395
2214	21	-1.7421397865532597
2214	22	-1.8601039434559183
2214	23	-2.480710857158627
2214	24	-1.553530026272135
2214	25	-1.7660544800350024
2215	1	-0.19350055330226468
2215	2	-0.2699555005346248
2215	3	-0.6983948052547824
2215	4	-0.14855080323880543
2215	5	-0.11505093849804382
2215	6	-0.13449750986836762
2215	7	-0.19920974127445135
2215	8	-0.1595750209366429
2215	9	-0.06560026601518842
2215	10	-0.0941797837284426
2215	11	-0.48480655868242195
2215	12	-0.3176882238396317
2215	13	-0.14049980356262637
2215	14	-0.13900732439263663
2215	15	-0.5118520539499087
2215	16	-0.230591793244308
2215	17	-0.048548433918092826
2215	18	-0.13643633008569367
2215	19	-2.016463224820093
2215	20	-2.1696539293231893
2215	21	-3.124083472775986
2215	22	-2.1815425537542694
2215	23	-3.332699761117645
2215	24	-2.079853378806419
2215	25	-1.4046916307663082
2216	1	-1.271481711943121
2216	2	-0.9991053682262709
2216	3	-0.6560961007348354
2216	4	-0.5174392246678963
2216	5	-1.3684838102362207
2216	6	-1.330415565808316
2216	7	-0.915735644370173
2216	8	-4.88298332119766
2216	9	-3.94526846416203
2216	10	-1.8993086723518005
2216	11	-0.8572319634269121
2216	12	-0.9188756214519742
2216	13	-1.747936341818594
2216	14	-3.0584376100210946
2216	15	-1.6963359993253917
2216	16	-2.6491115384303754
2216	17	-2.368950680875436
2216	18	-2.3897053711590033
2216	19	-1.426331326562888
2216	20	-0.7379320763213499
2216	21	-0.5899623540352863
2216	22	-0.5151497188373995
2216	23	-0.9341346221389699
2216	24	-0.2722658852688298
2216	25	-0.15696655069589127
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	70.0
8	369.0
9	960.0
10	1858.0
11	3381.0
12	5468.0
13	7728.0
14	10398.0
15	12972.0
16	14348.0
17	15390.0
18	15983.0
19	16503.0
20	16698.0
21	17870.0
22	18850.0
23	19828.0
24	20685.0
25	22361.0
26	24102.0
27	27037.0
28	30554.0
29	36138.0
30	43069.0
31	52810.0
32	70086.0
33	98456.0
34	141528.0
35	224523.0
36	389384.0
37	1278990.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.87983669965886	20.242880443313112	13.069941772691324	23.807341084336713
2	15.67013935878205	20.147679444854475	40.09808905269917	24.0840921436643
3	19.133706482985552	24.994721189732	28.619415786391095	27.252156540891352
4	12.812661590788812	16.755590567419656	36.88743348714628	33.54431435464525
5	13.421874366203376	37.97608906873076	34.8847347844267	13.717301780639174
6	32.29384978869558	37.69281125278937	16.61195002382363	13.401388934691413
7	27.439866795585576	32.438291948266176	21.773183325493843	18.348657930654415
8	24.923484287699836	37.471151444446086	19.81911401343341	17.786250254420665
9	24.44920162283122	17.64567426939241	21.170829366210423	36.73429474156595
10	14.923953219707881	30.63111550438603	32.95989654241851	21.48503473348757
11	33.204036281505736	25.285697338014508	22.280128367184425	19.230138013295335
12	21.385234490279146	27.160854141895317	30.569189139812536	20.884722228012993
13	27.263186142184964	23.37793717105238	25.886182892411814	23.47269379435084
14	20.74950367383696	23.241519626013783	27.302449300443527	28.706527399705728
15	23.37088829971706	30.666532744835322	23.35076212261049	22.61181683283713
16	22.13473027337665	30.5651891455909	25.511780522434957	21.788300058597496
17	21.012742093329827	29.889794423922293	27.546618292852106	21.550845189895774
18	21.338677582580022	29.23611348001592	28.422233592965302	21.00297534443876
19	21.67318956427347	28.726636390718912	28.66368152189321	20.936492523114406
20	22.423746942259395	28.366471016750396	28.226233765643087	20.983548275347125
21	22.546701939847946	28.146458557931798	27.938165212925036	21.368674289295218
22	21.959774710460152	28.02914895844555	28.165251273398752	21.84582505769555
23	21.96759455949115	28.247296132650334	28.11710992692579	21.667999380932727
24	21.94752451793244	28.25993272374094	28.070914862367935	21.721627895958687
25	22.23868814893944	27.881773548723356	27.643357397814594	22.236180904522612
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3098.0
1	3098.0
2	3286.0
3	3474.0
4	3474.0
5	3474.0
6	4669.5
7	5865.0
8	5865.0
9	5865.0
10	8424.0
11	10983.0
12	10983.0
13	10983.0
14	17393.5
15	23804.0
16	23804.0
17	23804.0
18	36778.0
19	49752.0
20	49752.0
21	49752.0
22	70602.0
23	91452.0
24	91452.0
25	91452.0
26	117713.0
27	143974.0
28	143974.0
29	143974.0
30	170964.5
31	197955.0
32	197955.0
33	197955.0
34	221356.0
35	244757.0
36	244757.0
37	244757.0
38	260995.0
39	277233.0
40	277233.0
41	277233.0
42	284028.5
43	290824.0
44	290824.0
45	290824.0
46	300644.5
47	310465.0
48	310465.0
49	310465.0
50	298997.0
51	287529.0
52	287529.0
53	287529.0
54	264768.0
55	242007.0
56	242007.0
57	242007.0
58	213893.5
59	185780.0
60	185780.0
61	185780.0
62	158184.5
63	130589.0
64	130589.0
65	130589.0
66	103219.5
67	75850.0
68	75850.0
69	75850.0
70	57075.5
71	38301.0
72	38301.0
73	38301.0
74	27284.0
75	16267.0
76	16267.0
77	16267.0
78	11016.0
79	5765.0
80	5765.0
81	5765.0
82	3840.5
83	1916.0
84	1916.0
85	1916.0
86	1241.5
87	567.0
88	567.0
89	567.0
90	359.5
91	152.0
92	152.0
93	152.0
94	91.5
95	31.0
96	31.0
97	31.0
98	20.0
99	9.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03051092727066679
2	0.014705887926731324
3	0.018723475865477512
4	0.021338698202963236
5	0.019936332601702775
6	0.009930264527844348
7	0.0018192851043378959
8	0.0019708921963660538
9	0.001781383331330856
10	8.717407791619085E-4
11	0.001137053190211185
12	0.0038659808467180284
13	0.0023120081534294093
14	4.927230490915134E-4
15	0.0016676780123097378
16	0.0023120081534294093
17	0.0021604010614012514
18	0.0018571868773449352
19	3.79017730070395E-5
20	4.927230490915134E-4
21	0.0838766236645784
22	0.08137510664611379
23	0.0824363562903109
24	9.096425521689479E-4
25	0.22843398591342706
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2638399.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.88798365119449
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.43391458912728	67.22617395488754
2	8.960801296023776	13.77955887100557
3	1.849027184398439	4.265039157739241
4	0.6637771306461357	2.041459406766275
5	0.30086459561569423	1.1566436054461369
6	0.1855543087353411	0.856013799387096
7	0.11593764287387318	0.623994811289107
8	0.07545331319855648	0.46411624893112546
9	0.059983827281878566	0.41508319782466374
>10	0.3237274862478798	4.561926239941807
>50	0.021572145470814423	1.1396304851383954
>100	0.007405839711198861	1.080208421047158
>500	0.0011388683847748647	0.6325640676642273
>1k	7.427402509034461E-4	1.2449119739875063
>5k	9.90320334537928E-5	0.5126757589441805
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	7086	0.2685719635278819	No Hit
CCCATGTACTCTGCGTTGATACCAC	6380	0.241813311784912	No Hit
GTATCAACGCAGAGTACTTTTTTTT	4334	0.16426628421250916	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3293	0.12481053851218105	No Hit
GAGTACATGGGAAGCAGTGGTATCA	3157	0.1196558973832237	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2648	0.10036389492264058	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	7.5803546014079E-5	0.0
10	0.0	0.0	0.0	7.5803546014079E-5	0.0
11	0.0	0.0	0.0	1.1370531902111849E-4	0.0
12	3.79017730070395E-5	0.0	0.0	1.51607092028158E-4	0.0
13	3.79017730070395E-5	0.0	0.0	1.51607092028158E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAACGAT	50	0.001493613	13.3075695	16
GTCCTAA	205	0.0	10.659345	1
TCTAGAC	285	0.0	10.331374	3
CTAGACA	295	0.0	10.30274	4
TGGACGG	115	1.0300213E-5	9.910601	5
CTAGACT	295	0.0	9.658818	4
GCGCGAT	90	0.0011082729	9.505406	16
TAGAGCG	80	0.004526403	9.497659	5
TATACTG	615	0.0	9.420443	5
GGACCGC	135	6.8786685E-6	9.145201	6
CTAAGAC	250	1.8189894E-12	9.118271	3
GGAACGA	105	4.4796342E-4	9.050536	15
GTGTACG	95	0.0018230766	9.000683	1
AATGCCG	95	0.001828431	8.997782	5
TTACACT	415	0.0	8.92568	4
TAAACTG	495	0.0	8.826107	5
AAACGGC	130	4.3403725E-5	8.774388	17
GTCTAGT	185	6.570008E-8	8.730392	1
CTATACT	460	0.0	8.67194	4
GTTCTAC	200	2.536217E-8	8.550649	1
>>END_MODULE