FastQCFastQC Report
Wed 25 May 2016
SRR1294709_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294709_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2638399
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT118860.4505004739616714No Hit
GGTATCAACGCAGAGTACTTTTTTT95030.36018054888589635No Hit
TATCAACGCAGAGTACTTTTTTTTT80900.3066253436269495No Hit
GTACATGGGAAGCAGTGGTATCAAC72610.2752047738041138No Hit
CCCATGTACTCTGCGTTGATACCAC69700.2641753578590653No Hit
ACGCAGAGTACTTTTTTTTTTTTTT48570.18408891149519083No Hit
GAGTACATGGGAAGCAGTGGTATCA38710.14671776331024988No Hit
CATGTACTCTGCGTTGATACCACTG26750.10138724279383066No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCCTA701.0973889E-412.20719516
GCACCGT1251.4206307E-912.1552446
AGACTCG550.00303714912.1079855
GTCGTAG657.90319E-411.7139351
CCGTACA855.357482E-511.16998317
GTACCGT700.001496875810.8528976
TCGTAGG750.002617378510.1478372
CTAGAAC2250.010.1476443
TGTGTCG750.00266140710.12745110
TGAACCG951.621552E-410.0141225
CTAGCAC2550.09.6999543
ATTAGAC2201.6370905E-119.5134163
TAGACAG3900.09.5134155
CTGTCGC1804.2255124E-99.4946659
CCTACAC3550.09.3794233
TTAGACA3400.09.233614
TCTGTCG1752.6959242E-89.2233898
TATACTG5400.09.1610675
TCCCGCA950.00180074699.012882
CTACGCC950.00180105829.012714
Produced by FastQC (version 0.11.5)