FastQCFastQC Report
Wed 25 May 2016
SRR1294708_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294708_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3782778
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC847672.240866368578859No Hit
CCCATGTACTCTGCGTTGATACCAC839652.219665018671463No Hit
GAGTACATGGGAAGCAGTGGTATCA372610.9850168315454938No Hit
CATGTACTCTGCGTTGATACCACTG311450.8233367118028073No Hit
GCGTTGATACCACTGCTTCCCATGT272770.7210838172369618No Hit
ACGCAGAGTACATGGGAAGCAGTGG211680.5595887466829933No Hit
GTATCAACGCAGAGTACATGGGAAG209890.5548567745714922No Hit
ACTCTGCGTTGATACCACTGCTTCC190140.5026464677546502No Hit
TATCAACGCAGAGTACATGGGAAGC188010.4970156852979477No Hit
GCTTCCCATGTACTCTGCGTTGATA151850.4014245615259473No Hit
GGTATCAACGCAGAGTACATGGGAA146730.3878895351511508No Hit
GTGGTATCAACGCAGAGTACATGGG105700.2794242749640608No Hit
CAGTGGTATCAACGCAGAGTACATG104750.27691289311717476No Hit
ATACCACTGCTTCCCATGTACTCTG102430.27077983429109503No Hit
GTACTCTGCGTTGATACCACTGCTT90890.24027315375102637No Hit
GCAGTGGTATCAACGCAGAGTACAT84320.22290496560993006No Hit
CTGCTTCCCATGTACTCTGCGTTGA79890.21119399552392445No Hit
TACCACTGCTTCCCATGTACTCTGC77810.2056953910591634No Hit
GGGAAGCAGTGGTATCAACGCAGAG76740.20286678203161804No Hit
CATGGGAAGCAGTGGTATCAACGCA75750.20024965779117887No Hit
ACATGGGAAGCAGTGGTATCAACGC75260.1989543134701534No Hit
GATACCACTGCTTCCCATGTACTCT69680.1842032495694963No Hit
GTTGATACCACTGCTTCCCATGTAC69630.1840710715775549No Hit
GCAGAGTACATGGGAAGCAGTGGTA69010.1824320644774819No Hit
CTTCCCATGTACTCTGCGTTGATAC65690.17365544581257478No Hit
CCACTGCTTCCCATGTACTCTGCGT65350.17275663546737344No Hit
AAGCAGTGGTATCAACGCAGAGTAC62070.16408575919601942No Hit
GTACATGGTAAGCAGTGGTATCAAC60250.1592744802893535No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA59460.1571860680166798No Hit
CCATGTACTCTGCGTTGATACCACT57910.15308855026649726No Hit
TTCCCATGTACTCTGCGTTGATACC51940.13730649802869743No Hit
ATGGGAAGCAGTGGTATCAACGCAG51620.13646055888027264No Hit
TGATACCACTGCTTCCCATGTACTC49650.13125274599778258No Hit
GTATCAACGCAGAGTACTTTTTTTT46680.12340137327646508No Hit
ACCATGTACTCTGCGTTGATACCAC44870.1186165299681874No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT456.787093E-414.7715686
ACGCTAA751.4863192E-512.6613446
CGCAAAA2100.012.2128662
TCGCCCG550.00308013312.08550814
AACGCTA658.046624E-411.6890945
TGCGAAT600.00589729811.07852912
GTATTAG3750.010.6410151
CTAGGAC2350.010.5091393
ACGCAAA2450.010.47038651
GTCCTAT2300.010.3270721
ACACCGC750.002658324810.1290756
CCGTACA2350.09.70844617
ACACCGT1002.768846E-49.4960086
AGACCGT1106.873187E-59.4960076
ATTTTCG1554.2414467E-79.19710215
GTGTAAG4350.09.1732881
CGGAAAT1054.515354E-49.04357814
TAACGCT950.00182796628.99823954
CTAGTAC1606.7618566E-78.904983
TTTCGTC1502.6805465E-68.8724417
Produced by FastQC (version 0.11.5)