##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294708_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3782778
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.085904591810568	33.0	33.0	33.0	27.0	33.0
2	31.109716457058806	33.0	33.0	33.0	27.0	33.0
3	31.066224874946403	33.0	33.0	33.0	27.0	33.0
4	34.502595447049764	37.0	37.0	37.0	27.0	37.0
5	34.7989141842318	37.0	37.0	37.0	27.0	37.0
6	34.96095118455273	37.0	37.0	37.0	33.0	37.0
7	34.9840981945015	37.0	37.0	37.0	33.0	37.0
8	34.91470950713999	37.0	37.0	37.0	33.0	37.0
9	34.59437561495811	37.0	37.0	37.0	27.0	37.0
10	34.724849039515405	37.0	37.0	37.0	27.0	37.0
11	34.952313352779356	37.0	37.0	37.0	33.0	37.0
12	34.88716149877154	37.0	37.0	37.0	33.0	37.0
13	34.76310425829906	37.0	37.0	37.0	27.0	37.0
14	35.985909032991096	40.0	37.0	40.0	27.0	40.0
15	36.24439684274361	40.0	37.0	40.0	27.0	40.0
16	36.25287553221469	40.0	37.0	40.0	27.0	40.0
17	36.246327170137924	40.0	37.0	40.0	27.0	40.0
18	36.23741282200542	40.0	37.0	40.0	27.0	40.0
19	36.26507767571874	40.0	37.0	40.0	27.0	40.0
20	36.18076318515123	40.0	37.0	40.0	27.0	40.0
21	36.11425518494609	40.0	37.0	40.0	27.0	40.0
22	36.01995623322331	40.0	37.0	40.0	27.0	40.0
23	35.652992853400335	37.0	37.0	40.0	27.0	40.0
24	35.98068430132564	37.0	37.0	40.0	27.0	40.0
25	34.910844622655624	37.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.5274569917913112
1101	2	0.6892771919523746
1101	3	0.5153143231519728
1101	4	0.5746192381696389
1101	5	0.5273335852816317
1101	6	0.42936606736498106
1101	7	0.5115731388478224
1101	8	0.5622234554195913
1101	9	0.754681738971577
1101	10	0.7923465845579329
1101	11	0.6217763088441046
1101	12	0.6320167818252713
1101	13	0.606002580242567
1101	14	1.3257797076275537
1101	15	1.0138031805298482
1101	16	1.0805082002114261
1101	17	1.0796876184455542
1101	18	1.0299373493845607
1101	19	1.0602691139932219
1101	20	1.1205770323742854
1101	21	1.1236273900677887
1101	22	1.1582541301515974
1101	23	1.5745643886118188
1101	24	1.328195550921528
1101	25	1.2540226388108593
1102	1	0.18454368208017513
1102	2	0.43376832072602056
1102	3	0.2400100644878762
1102	4	0.09484761037202105
1102	5	0.16805163523802236
1102	6	0.18252937362247934
1102	7	0.23983184445724248
1102	8	0.3603027337413849
1102	9	0.5666146714582823
1102	10	0.5542393952156459
1102	11	0.3466991839807534
1102	12	0.35170257141886907
1102	13	0.37065136049057656
1102	14	1.0516263350202024
1102	15	0.8053617061923575
1102	16	0.6066779901256396
1102	17	0.8047213256205055
1102	18	0.7237546397014611
1102	19	0.6833009512237709
1102	20	0.771740607489761
1102	21	0.7597455773423079
1102	22	0.9041760125333127
1102	23	1.1557487690151476
1102	24	0.877709667613324
1102	25	0.8093342423327172
1103	1	0.20353710920097967
1103	2	0.45105105271468204
1103	3	0.1711164011743911
1103	4	0.018211916470583844
1103	5	0.2921944015034015
1103	6	0.17001596817025444
1103	7	0.2033574072131259
1103	8	0.3775500296584582
1103	9	0.5711780044918768
1103	10	0.5336354703327473
1103	11	0.3348113719888417
1103	12	0.3772414301438545
1103	13	0.3386441214268885
1103	14	0.9432790781783922
1103	15	0.77737454948101
1103	16	0.45501160373849814
1103	17	0.4858555707738006
1103	18	0.6031382063632336
1103	19	0.6813329519764579
1103	20	0.7487750971656055
1103	21	0.7991676948995234
1103	22	0.6778523438252151
1103	23	1.1739423241154725
1103	24	0.7344027461019067
1103	25	0.7404987856686063
1104	1	0.23966052710178687
1104	2	0.4388861196615892
1104	3	0.2912055141767347
1104	4	0.2413861532485626
1104	5	0.1465188310134593
1104	6	0.1857379289430483
1104	7	0.18125074124328222
1104	8	0.3737834929598165
1104	9	0.45387956958869324
1104	10	0.44000230020520803
1104	11	0.2718460614495939
1104	12	0.2677935695519622
1104	13	0.349063377568271
1104	14	0.8256969042937996
1104	15	0.6417218792936978
1104	16	0.5753331190850304
1104	17	0.6648386752390039
1104	18	0.5752931680839097
1104	19	0.6321970840161413
1104	20	0.7003544734347145
1104	21	0.7295189091844279
1104	22	0.8008299520327853
1104	23	1.1994101523467862
1104	24	0.9993763366395001
1104	25	0.808887048818363
1105	1	0.2307818421099519
1105	2	0.31960116388357207
1105	3	0.29273304513531784
1105	4	0.3496651833122115
1105	5	0.3592881589040289
1105	6	0.18668968433758693
1105	7	0.23708432668674817
1105	8	0.32948812725636856
1105	9	-1.6632045365123247
1105	10	-0.1629965453728488
1105	11	0.07104912355629267
1105	12	0.21458052094873636
1105	13	0.30139580567059454
1105	14	-1.623288751733746
1105	15	-0.2872144490574655
1105	16	-0.060373617034258587
1105	17	0.12706162114999842
1105	18	0.21157467982087041
1105	19	0.3603403774904308
1105	20	0.46329080365674
1105	21	0.49488585095139115
1105	22	0.43207979821039544
1105	23	0.8998067084928181
1105	24	0.6851923201239956
1105	25	0.5267052187662102
1106	1	0.08217084004944653
1106	2	0.15438264132898283
1106	3	0.16047273141661478
1106	4	-1.0790727463036376
1106	5	-0.22475953179399966
1106	6	0.08769713937255119
1106	7	0.11656098987305796
1106	8	0.38151611540994423
1106	9	0.48364193886504125
1106	10	0.5308838477947688
1106	11	0.3942650212907637
1106	12	0.2765294139388814
1106	13	0.29387340699570075
1106	14	0.696482041628343
1106	15	0.47304390548921305
1106	16	0.32453303133299727
1106	17	0.23226457015960023
1106	18	0.34806136361543594
1106	19	0.3350507989990632
1106	20	0.38185842610523224
1106	21	0.5332608630268538
1106	22	0.6269490287183217
1106	23	0.9331066891177642
1106	24	0.4615744099273087
1106	25	0.37782524118924954
1107	1	0.4279488302004104
1107	2	0.3857077321568525
1107	3	0.42719846437586995
1107	4	0.6132680383611273
1107	5	0.5759961246540755
1107	6	0.3365819226849567
1107	7	0.3390822814483627
1107	8	0.3959613444298995
1107	9	0.5619128244501681
1107	10	0.49968829393118597
1107	11	0.2921515838770219
1107	12	0.29812316714636466
1107	13	0.32120482157085917
1107	14	0.9212147529233619
1107	15	0.6314856309618548
1107	16	0.7127425205468754
1107	17	0.6086900174258076
1107	18	0.6323860369057215
1107	19	0.6219934066328463
1107	20	0.7685986956429645
1107	21	0.7302721604946711
1107	22	0.7233334629384913
1107	23	0.983821657868809
1107	24	0.8767750512882273
1107	25	0.7046652109367528
1108	1	0.3546942145582257
1108	2	0.31573972717847454
1108	3	0.3756885945865456
1108	4	0.5019793507573596
1108	5	0.4066928480298131
1108	6	0.27632337215152347
1108	7	0.24203686953708115
1108	8	0.2590237275629619
1108	9	0.08642788956931469
1108	10	0.36823364300951056
1108	11	0.17706471849443517
1108	12	0.27017136820136045
1108	13	0.2126538556840316
1108	14	-1.2317293145516075
1108	15	-0.09274484337864664
1108	16	0.2483725504995533
1108	17	0.3547726968760401
1108	18	0.4052666638078364
1108	19	0.4030049234887656
1108	20	0.49729223601519124
1108	21	0.5745038079108582
1108	22	0.5913341022429393
1108	23	0.9703432071747287
1108	24	0.8786415789689244
1108	25	0.8364406769817734
1109	1	0.3667474323813522
1109	2	0.2749575517917755
1109	3	0.4092491140279755
1109	4	0.4545239634356477
1109	5	0.5110621948811414
1109	6	0.3626084753269865
1109	7	0.28937670167272955
1109	8	0.3247332892943291
1109	9	0.5159019179265911
1109	10	0.4850078762499592
1109	11	0.3345887933968328
1109	12	0.33836753916791906
1109	13	0.3337878528717084
1109	14	0.6591458796151315
1109	15	0.4756472739623021
1109	16	0.4427867751885799
1109	17	0.3987628008694202
1109	18	0.36139920497730316
1109	19	0.33664454191307414
1109	20	0.6509095050030211
1109	21	0.5284600406837114
1109	22	0.6615753026812143
1109	23	1.044107439945762
1109	24	0.7874794580160938
1109	25	0.6407892513446782
1110	1	0.2828623124341867
1110	2	0.07936871472819007
1110	3	0.24986275733204977
1110	4	0.41474300171623213
1110	5	0.3216322483775116
1110	6	0.1130805698192674
1110	7	0.11166571505789591
1110	8	0.12091239024314149
1110	9	0.3281340097987453
1110	10	0.32605164748773063
1110	11	0.1836651390098396
1110	12	0.1400044293126328
1110	13	0.098809254920603
1110	14	0.5196949960098891
1110	15	0.3264526776284882
1110	16	0.23280841915342165
1110	17	0.29595899916925816
1110	18	0.3408718579852774
1110	19	0.24474333371453127
1110	20	0.3097936827875074
1110	21	0.4262466241713625
1110	22	0.5057263774400198
1110	23	0.7222795280062542
1110	24	0.6154149412520908
1110	25	0.656306636224187
1111	1	0.10510994318039124
1111	2	0.06655968810852642
1111	3	0.29308018598520036
1111	4	0.5649035510682623
1111	5	0.29930999164024286
1111	6	0.28449653992917234
1111	7	0.15444946196470966
1111	8	0.33492480320192186
1111	9	0.1941012742440904
1111	10	0.20653880380352518
1111	11	0.1207384719271758
1111	12	0.08026523068048164
1111	13	0.10290270380808408
1111	14	-1.8203206788268815
1111	15	-0.5843050158540137
1111	16	-0.15640780310955193
1111	17	0.03677632270407827
1111	18	0.08530069727765976
1111	19	0.15952201982196357
1111	20	0.17854411237791368
1111	21	0.3564043829674475
1111	22	0.07814470933373485
1111	23	-2.0872750061403664
1111	24	-0.4509426420441045
1111	25	-0.028340824068990855
1112	1	-0.14620956096266724
1112	2	-0.23449345121217036
1112	3	0.02385282141815992
1112	4	0.15253812517961762
1112	5	-0.7552008001453885
1112	6	-0.8515490218502464
1112	7	-0.8480179286496821
1112	8	-0.7730782282201858
1112	9	-0.6710643233737628
1112	10	-0.8374783151732998
1112	11	-0.8380673043231255
1112	12	-0.838557886813085
1112	13	-0.8548028941626384
1112	14	-0.648174110338708
1112	15	-0.8767581578922048
1112	16	-0.8896883380253584
1112	17	-0.9923695799987868
1112	18	-1.0049661420916536
1112	19	-0.9328797231037882
1112	20	-0.900345362596731
1112	21	-1.864938710620308
1112	22	-1.7282649293231032
1112	23	-1.4480538836908252
1112	24	-1.700529694943313
1112	25	-2.0798420959156445
1113	1	0.1498826491875711
1113	2	-0.045942912275378944
1113	3	0.025233603639104984
1113	4	0.23854219899581608
1113	5	0.13922377244854545
1113	6	0.05118396424551008
1113	7	0.03403806079156624
1113	8	0.16199950076251213
1113	9	0.2249007208278968
1113	10	-0.06773492827653627
1113	11	-0.12015927598138632
1113	12	-0.06361412422445767
1113	13	-0.07517794488436635
1113	14	-0.013953667228946642
1113	15	-0.09963206466129293
1113	16	-0.010929155327353612
1113	17	0.02003393830874245
1113	18	0.032011677211322365
1113	19	-0.17880155241965667
1113	20	-0.630408294010536
1113	21	-0.5076152972829888
1113	22	0.018362451173977945
1113	23	-0.21477421353426962
1113	24	0.04460740841924604
1113	25	-1.1745534841458962
1114	1	-0.9531632073712295
1114	2	-0.7188359883020574
1114	3	-0.4994061226615507
1114	4	-1.6806614343724036
1114	5	-0.8632962794815668
1114	6	-0.6532497125651844
1114	7	-0.5932404743185344
1114	8	-0.48736842611475595
1114	9	-0.445252878901492
1114	10	-0.5926586283845481
1114	11	-0.6367059795663508
1114	12	-0.6321060726839249
1114	13	-0.6887551142745636
1114	14	-0.6239549871277319
1114	15	-1.3481671604251346
1114	16	-0.8803092209977237
1114	17	-0.749456779727133
1114	18	-0.7011930722767232
1114	19	-0.7511327227758287
1114	20	-2.034015803581134
1114	21	-6.592543581202964
1114	22	-7.629722528249033
1114	23	-7.743705809518325
1114	24	-5.800872990160553
1114	25	-7.26169377072938
1115	1	-1.0233875998129243
1115	2	-0.9609137596820325
1115	3	-0.728956816978755
1115	4	-0.6204172686046263
1115	5	-0.6574597160795008
1115	6	-0.7788446308881021
1115	7	-0.9800464281129564
1115	8	-0.7983083755070837
1115	9	-2.7299322123776335
1115	10	-1.4085818827212009
1115	11	-1.0635061612079326
1115	12	-1.0361376495948704
1115	13	-1.3401912320399276
1115	14	-0.9776320574892949
1115	15	-1.9281332300270293
1115	16	-1.2991967106408069
1115	17	-3.1484272484519593
1115	18	-2.763899391541763
1115	19	-2.1376495330260994
1115	20	-1.7775728596409266
1115	21	-1.294257279206633
1115	22	-0.8906073211352918
1115	23	-0.2027280064373045
1115	24	-1.3440863819448907
1115	25	-1.0459015347134155
1116	1	-1.737352635685454
1116	2	-0.9378842274152959
1116	3	-0.7486246763065374
1116	4	-0.520705954390948
1116	5	-0.464614372710507
1116	6	-0.5299063464662339
1116	7	-1.0623442967719257
1116	8	-1.967621118442267
1116	9	-1.063150605313126
1116	10	-2.509375737735631
1116	11	-1.2540023947470473
1116	12	-0.9458322418345375
1116	13	-0.9602963592468114
1116	14	-1.445224377493247
1116	15	-1.5334127267611137
1116	16	-0.9885425267485246
1116	17	-0.7386312861968989
1116	18	-1.4537348537442938
1116	19	-1.0857063605475261
1116	20	-1.3775651475110067
1116	21	-0.8184770254726175
1116	22	-0.35578411306227764
1116	23	-0.35892501180737213
1116	24	-0.29813021996544364
1116	25	-0.45274590170698303
1201	1	0.2788219752570953
1201	2	0.4936222160938897
1201	3	0.25837272453954796
1201	4	0.4380161753927396
1201	5	0.4571605340353315
1201	6	0.36341045668320504
1201	7	0.3196331047266696
1201	8	0.45740531897685344
1201	9	0.7408949396935611
1201	10	0.685746814002755
1201	11	0.5431547616263188
1201	12	0.517485844579177
1201	13	0.5630317757786898
1201	14	1.2984676390804353
1201	15	0.9395548110027434
1201	16	0.8901943061987367
1201	17	0.8444967031019814
1201	18	0.8186476025105307
1201	19	0.7991413333830693
1201	20	0.9370594106871977
1201	21	0.9574632048624423
1201	22	1.0089624857436377
1201	23	0.9349626046923589
1201	24	0.8276937988267647
1201	25	0.7347448381274972
1202	1	-0.31340097667133193
1202	2	-0.037041985363835295
1202	3	-0.14054904605443497
1202	4	-0.08816839916267583
1202	5	-0.07253262042196695
1202	6	-0.1702870967044703
1202	7	-0.11637707276891973
1202	8	0.1276287588282301
1202	9	0.18996311440156433
1202	10	0.30549177215492307
1202	11	0.13871775310590806
1202	12	0.14473156306886636
1202	13	0.14749064008900348
1202	14	0.3515129236127592
1202	15	0.39179914767938584
1202	16	0.19100016868016212
1202	17	0.1731687657826555
1202	18	0.19823927198847002
1202	19	0.2416298316613137
1202	20	0.36807783958073514
1202	21	0.4194406446882297
1202	22	0.31436495274961374
1202	23	0.6209217640163587
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2211	6	0.32253111127548806
2211	7	0.2822196728772397
2211	8	0.3979484856445765
2211	9	0.5553923291020197
2211	10	0.4529860682894622
2211	11	0.2745663829556051
2211	12	0.3802969746029987
2211	13	0.26600925265312014
2211	14	0.5768659237613534
2211	15	0.43012554985221385
2211	16	0.2908114661896448
2211	17	0.44848207868928824
2211	18	0.34442892057769825
2211	19	0.42297442977626787
2211	20	0.46470357760355796
2211	21	0.483106927648862
2211	22	0.49916878303869083
2211	23	0.6497182907243655
2211	24	0.581461996997966
2211	25	0.757467981547471
2212	1	-0.16169189697136233
2212	2	-0.19196296278541425
2212	3	-0.1009311058550999
2212	4	-0.3714277459499513
2212	5	-0.1692568209042591
2212	6	-0.5607094067084191
2212	7	-0.4466949073003761
2212	8	-0.22423438286948283
2212	9	-0.08961866023535237
2212	10	-0.4631875632410285
2212	11	-0.371904406197622
2212	12	-0.49022338537205457
2212	13	-0.5617774353982057
2212	14	-0.3496451101273905
2212	15	-0.4716639300848229
2212	16	-0.2931403886322954
2212	17	-0.46208105059878335
2212	18	-0.35558741492380364
2212	19	-0.4179757023927877
2212	20	-0.41037215972157526
2212	21	-0.355320112061257
2212	22	-0.3704278785788162
2212	23	0.01420119784961571
2212	24	-0.23854750729380925
2212	25	-0.8714243229462824
2213	1	-0.23103158766326715
2213	2	-1.4189229078998977
2213	3	-0.3873808432894599
2213	4	-0.01412779061924141
2213	5	-0.00194138093315388
2213	6	-0.11137693470152499
2213	7	-0.08616962640750359
2213	8	0.04437681373308777
2213	9	-0.09661773906488946
2213	10	-0.43574456467086975
2213	11	-0.27006674058463176
2213	12	-0.07382326504282588
2213	13	-0.410185450863608
2213	14	-0.04442585659605669
2213	15	-0.17464106325700612
2213	16	-0.15969938949362472
2213	17	0.029536645491901936
2213	18	-0.038357706855762785
2213	19	-0.07919757837460395
2213	20	0.05236646831149727
2213	21	-0.5454332119174197
2213	22	-0.9447872269629229
2213	23	-0.15603137097635056
2213	24	0.014870120617437976
2213	25	-0.6672839983352148
2214	1	-0.29848866509721717
2214	2	-0.4344969725133332
2214	3	-0.43333178414967577
2214	4	-0.6272047289007574
2214	5	-0.24936192173011307
2214	6	-0.289685758230938
2214	7	-0.5298430712817321
2214	8	-0.13798631452069543
2214	9	-0.23481114452881258
2214	10	-2.108685786755238
2214	11	-0.892361463374499
2214	12	-0.5439192516384566
2214	13	-0.32788336905073123
2214	14	-0.901794230434426
2214	15	-0.5584764522513126
2214	16	-0.6984555169446054
2214	17	-0.7143408450204305
2214	18	-0.5885412262933158
2214	19	-1.466312978194587
2214	20	-2.4254401475110114
2214	21	-1.9687784960608496
2214	22	-1.9945488189446294
2214	23	-2.5697019725916874
2214	24	-1.6364733572203463
2214	25	-2.0854456566089468
2215	1	-0.20101634525280687
2215	2	-0.2891865339527584
2215	3	-0.6778515540646026
2215	4	-0.1356665112354989
2215	5	-0.15102827562666477
2215	6	-0.12017430515782479
2215	7	-0.10482025327981859
2215	8	-0.0911044904453604
2215	9	-0.15959753675391397
2215	10	-0.18249306633760654
2215	11	-0.4723826322248641
2215	12	-0.2781840001712794
2215	13	-0.17159775590756254
2215	14	0.010024428293569088
2215	15	-0.5683079544577581
2215	16	-0.3490458705454387
2215	17	-0.19213494845066492
2215	18	-0.1576078349553427
2215	19	-2.053187366871171
2215	20	-2.297935285357333
2215	21	-3.317022860511969
2215	22	-2.30167884032673
2215	23	-3.321761279493103
2215	24	-2.2619811821600706
2215	25	-1.6024810365962026
2216	1	-1.5442264541676032
2216	2	-1.227442087376751
2216	3	-0.8785532805095286
2216	4	-1.0156014302099834
2216	5	-1.855771082508312
2216	6	-1.5837248286172851
2216	7	-1.2259796704221628
2216	8	-5.432417790309142
2216	9	-4.135231606200218
2216	10	-2.2008294100001677
2216	11	-1.1580914712512822
2216	12	-1.1813546792026202
2216	13	-2.1508098339034447
2216	14	-3.5572257234294398
2216	15	-2.1453747958321117
2216	16	-3.2333733364515
2216	17	-2.8136122901429417
2216	18	-2.7664647182329887
2216	19	-1.7605404127331425
2216	20	-1.0488023687645693
2216	21	-0.8720971655723417
2216	22	-0.8569321966326768
2216	23	-1.136201448747201
2216	24	-0.6475501826463059
2216	25	-0.479251912535652
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	6.0
7	38.0
8	185.0
9	613.0
10	1447.0
11	2900.0
12	5019.0
13	7634.0
14	11068.0
15	15257.0
16	19066.0
17	20575.0
18	21356.0
19	21810.0
20	22471.0
21	23700.0
22	24886.0
23	25942.0
24	27947.0
25	30090.0
26	32312.0
27	35483.0
28	41015.0
29	48331.0
30	58145.0
31	71890.0
32	97114.0
33	140279.0
34	204810.0
35	326000.0
36	571062.0
37	1874327.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.58957814592373	19.586830096953875	12.284350083245362	24.539241673877033
2	14.009246465418823	21.785536573667933	37.94829044033079	26.256926520582454
3	19.773670853677434	27.062882369023487	28.26882713813328	24.8946196391658
4	12.89125049973876	18.6131535472462	34.66632257846163	33.82927337455341
5	12.29195718494334	39.33240684774383	35.155765389734725	13.219870577578096
6	32.22314429540581	35.27805000820877	17.825143375917605	14.673662320467812
7	25.62113792123507	33.45546589474658	22.54056876235039	18.382827421667955
8	26.101146754976064	36.32320413581508	20.84427221280964	16.731376896399215
9	26.041538618119908	17.489072089183914	20.25092888894176	36.21846040375441
10	15.44350591947484	31.26525338059326	32.70010214742221	20.591138552509697
11	30.755292983083184	26.426609889540504	21.309319372392103	21.508777754984212
12	22.615886003903004	28.93865429322663	29.45735429951839	18.988105403351973
13	28.13405444616927	22.625937432587335	24.376313886057265	24.86369423518613
14	21.152265093352522	25.169228377813223	26.14700702503306	27.531499503801193
15	27.42800161472434	27.699818832581798	22.379238684438505	22.49294086825536
16	20.54848986577495	29.41601939578769	28.536695044276843	21.49879569416051
17	23.506930014624565	29.774847224557426	27.00453329864889	19.71368946216911
18	24.062267857001235	27.256026795745885	28.227108221926724	20.454597125326156
19	22.37121106578558	30.252707084961006	28.485161227559036	18.890920621694377
20	23.2380977054308	27.94984506013866	27.34134723923656	21.47070999519398
21	20.98499382338288	28.85476337695991	28.909901822346782	21.250340977310426
22	22.100515429823815	28.468171771843593	24.77690370653359	24.654409091799
23	20.30999113426626	28.92025628346643	27.55957384156132	23.210178740705995
24	21.92048874689874	31.62399692286137	25.50947925244565	20.946035077794242
25	22.351141915233786	26.765600681275366	24.7821863661198	26.10107103737105
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	8019.0
1	8019.0
2	9097.5
3	10176.0
4	10176.0
5	10176.0
6	12657.5
7	15139.0
8	15139.0
9	15139.0
10	17382.0
11	19625.0
12	19625.0
13	19625.0
14	26530.5
15	33436.0
16	33436.0
17	33436.0
18	47845.5
19	62255.0
20	62255.0
21	62255.0
22	86082.0
23	109909.0
24	109909.0
25	109909.0
26	140218.0
27	170527.0
28	170527.0
29	170527.0
30	202029.0
31	233531.0
32	233531.0
33	233531.0
34	260099.0
35	286667.0
36	286667.0
37	286667.0
38	307508.5
39	328350.0
40	328350.0
41	328350.0
42	357506.0
43	386662.0
44	386662.0
45	386662.0
46	544005.0
47	701348.0
48	701348.0
49	701348.0
50	627625.5
51	553903.0
52	553903.0
53	553903.0
54	441254.5
55	328606.0
56	328606.0
57	328606.0
58	275596.0
59	222586.0
60	222586.0
61	222586.0
62	187967.5
63	153349.0
64	153349.0
65	153349.0
66	120504.5
67	87660.0
68	87660.0
69	87660.0
70	65255.0
71	42850.0
72	42850.0
73	42850.0
74	30780.5
75	18711.0
76	18711.0
77	18711.0
78	12544.5
79	6378.0
80	6378.0
81	6378.0
82	4306.0
83	2234.0
84	2234.0
85	2234.0
86	1428.5
87	623.0
88	623.0
89	623.0
90	402.5
91	182.0
92	182.0
93	182.0
94	107.5
95	33.0
96	33.0
97	33.0
98	26.0
99	19.0
100	19.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03135261968849348
2	0.015226904671646077
3	0.020196797168641673
4	0.0212013499073961
5	0.019668085200876182
6	0.0063181080147975905
7	7.930679516482331E-5
8	4.494051726006655E-4
9	8.723747468130564E-4
10	2.643559838827444E-4
11	8.98810345201331E-4
12	0.0013746511161902707
13	0.0028021734291570903
14	5.287119677654888E-4
15	0.002352768256556425
16	0.002669995437215718
17	0.002696431035603993
18	0.0023263326581681506
19	5.022763693772143E-4
20	5.287119677654888E-4
21	0.08462035044086647
22	0.0798355071325888
23	0.08081362427295495
24	0.0011367307306958008
25	0.22946099401022213
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3782778.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.69102322051158
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.71887343117692	40.17368091202967
2	12.997690422365077	13.437278348709883
3	4.366226903214613	6.770842087202668
4	1.8538921007552407	3.8331831851384854
5	0.927899142156873	2.398202805176185
6	0.5169749136482646	1.603377735948865
7	0.34196364307753646	1.237351543042417
8	0.23124798438889774	0.9562755944594409
9	0.1584052540458977	0.736931669862378
>10	0.8036158825204405	7.350648577958975
>50	0.0506713633399111	1.8081654883202205
>100	0.026786440642724466	2.655702159265733
>500	0.001969330718257164	0.7063888697274129
>1k	0.00212480419072123	2.137757900897403
>5k	9.328408642190765E-4	3.3305475688885027
>10k+	7.25542894392615E-4	10.863665553371844
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	84767	2.240866368578859	No Hit
CCCATGTACTCTGCGTTGATACCAC	83965	2.219665018671463	No Hit
GAGTACATGGGAAGCAGTGGTATCA	37261	0.9850168315454938	No Hit
CATGTACTCTGCGTTGATACCACTG	31145	0.8233367118028073	No Hit
GCGTTGATACCACTGCTTCCCATGT	27277	0.7210838172369618	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	21168	0.5595887466829933	No Hit
GTATCAACGCAGAGTACATGGGAAG	20989	0.5548567745714922	No Hit
ACTCTGCGTTGATACCACTGCTTCC	19014	0.5026464677546502	No Hit
TATCAACGCAGAGTACATGGGAAGC	18801	0.4970156852979477	No Hit
GCTTCCCATGTACTCTGCGTTGATA	15185	0.4014245615259473	No Hit
GGTATCAACGCAGAGTACATGGGAA	14673	0.3878895351511508	No Hit
GTGGTATCAACGCAGAGTACATGGG	10570	0.2794242749640608	No Hit
CAGTGGTATCAACGCAGAGTACATG	10475	0.27691289311717476	No Hit
ATACCACTGCTTCCCATGTACTCTG	10243	0.27077983429109503	No Hit
GTACTCTGCGTTGATACCACTGCTT	9089	0.24027315375102637	No Hit
GCAGTGGTATCAACGCAGAGTACAT	8432	0.22290496560993006	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	7989	0.21119399552392445	No Hit
TACCACTGCTTCCCATGTACTCTGC	7781	0.2056953910591634	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	7674	0.20286678203161804	No Hit
CATGGGAAGCAGTGGTATCAACGCA	7575	0.20024965779117887	No Hit
ACATGGGAAGCAGTGGTATCAACGC	7526	0.1989543134701534	No Hit
GATACCACTGCTTCCCATGTACTCT	6968	0.1842032495694963	No Hit
GTTGATACCACTGCTTCCCATGTAC	6963	0.1840710715775549	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	6901	0.1824320644774819	No Hit
CTTCCCATGTACTCTGCGTTGATAC	6569	0.17365544581257478	No Hit
CCACTGCTTCCCATGTACTCTGCGT	6535	0.17275663546737344	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	6207	0.16408575919601942	No Hit
GTACATGGTAAGCAGTGGTATCAAC	6025	0.1592744802893535	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	5946	0.1571860680166798	No Hit
CCATGTACTCTGCGTTGATACCACT	5791	0.15308855026649726	No Hit
TTCCCATGTACTCTGCGTTGATACC	5194	0.13730649802869743	No Hit
ATGGGAAGCAGTGGTATCAACGCAG	5162	0.13646055888027264	No Hit
TGATACCACTGCTTCCCATGTACTC	4965	0.13125274599778258	No Hit
GTATCAACGCAGAGTACTTTTTTTT	4668	0.12340137327646508	No Hit
ACCATGTACTCTGCGTTGATACCAC	4487	0.1186165299681874	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	5.2871196776548875E-5	0.0
4	0.0	0.0	0.0	1.5861359032964662E-4	0.0
5	0.0	2.6435598388274437E-5	0.0	1.8504918871792107E-4	0.0
6	0.0	2.6435598388274437E-5	0.0	1.8504918871792107E-4	0.0
7	0.0	2.6435598388274437E-5	0.0	1.8504918871792107E-4	0.0
8	0.0	2.6435598388274437E-5	0.0	1.8504918871792107E-4	0.0
9	0.0	2.6435598388274437E-5	0.0	2.114847871061955E-4	0.0
10	0.0	2.6435598388274437E-5	0.0	2.907915822710188E-4	0.0
11	0.0	2.6435598388274437E-5	0.0	4.22969574212391E-4	0.0
12	0.0	5.2871196776548875E-5	0.0	4.758407709889399E-4	0.0
13	0.0	5.2871196776548875E-5	0.0	5.022763693772143E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAACCGT	45	6.787093E-4	14.771568	6
ACGCTAA	75	1.4863192E-5	12.661344	6
CGCAAAA	210	0.0	12.212866	2
TCGCCCG	55	0.003080133	12.085508	14
AACGCTA	65	8.046624E-4	11.689094	5
TGCGAAT	60	0.005897298	11.078529	12
GTATTAG	375	0.0	10.641015	1
CTAGGAC	235	0.0	10.509139	3
ACGCAAA	245	0.0	10.4703865	1
GTCCTAT	230	0.0	10.327072	1
ACACCGC	75	0.0026583248	10.129075	6
CCGTACA	235	0.0	9.708446	17
ACACCGT	100	2.768846E-4	9.496008	6
AGACCGT	110	6.873187E-5	9.496007	6
ATTTTCG	155	4.2414467E-7	9.197102	15
GTGTAAG	435	0.0	9.173288	1
CGGAAAT	105	4.515354E-4	9.043578	14
TAACGCT	95	0.0018279662	8.9982395	4
CTAGTAC	160	6.7618566E-7	8.90498	3
TTTCGTC	150	2.6805465E-6	8.87244	17
>>END_MODULE