FastQCFastQC Report
Wed 25 May 2016
SRR1294708_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294708_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3782778
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC906922.3974972890293853No Hit
GTACATGGGAAGCAGTGGTATCAAC834292.205495537935348No Hit
GAGTACATGGGAAGCAGTGGTATCA413211.092345361001888No Hit
CATGTACTCTGCGTTGATACCACTG352300.9313261312189085No Hit
GCGTTGATACCACTGCTTCCCATGT289690.7658128497099222No Hit
ACGCAGAGTACATGGGAAGCAGTGG260460.688541595620996No Hit
GTATCAACGCAGAGTACATGGGAAG226560.5989249170847456No Hit
TATCAACGCAGAGTACATGGGAAGC224170.592606809069948No Hit
ACTCTGCGTTGATACCACTGCTTCC216290.5717755575399879No Hit
GGTATCAACGCAGAGTACATGGGAA166200.43935964521312115No Hit
GCTTCCCATGTACTCTGCGTTGATA161800.42772798192228045No Hit
GTATCAACGCAGAGTACTTTTTTTT138810.3669525412276375No Hit
CAGTGGTATCAACGCAGAGTACATG127890.3380848677876418No Hit
ATACCACTGCTTCCCATGTACTCTG114130.30170948440537615No Hit
GTGGTATCAACGCAGAGTACATGGG112730.2980085006310177No Hit
TATCAACGCAGAGTACTTTTTTTTT102960.27218092100567365No Hit
CATGGGAAGCAGTGGTATCAACGCA102600.27122923946369576No Hit
GGTATCAACGCAGAGTACTTTTTTT98840.26128945446970453No Hit
ACATGGGAAGCAGTGGTATCAACGC95550.25259214259996227No Hit
GCAGTGGTATCAACGCAGAGTACAT95190.2516404610579843No Hit
GGGAAGCAGTGGTATCAACGCAGAG92790.2452959174447985No Hit
GTACTCTGCGTTGATACCACTGCTT91260.24125127089139253No Hit
TACCACTGCTTCCCATGTACTCTGC87740.2319459402587199No Hit
CTGCTTCCCATGTACTCTGCGTTGA87630.2316551486764489No Hit
GCAGAGTACATGGGAAGCAGTGGTA80360.2124364686481734No Hit
GATACCACTGCTTCCCATGTACTCT79480.21011013599000522No Hit
CTTCCCATGTACTCTGCGTTGATAC78750.2081803373076612No Hit
AAGCAGTGGTATCAACGCAGAGTAC77480.20482301631235034No Hit
GTTGATACCACTGCTTCCCATGTAC72960.1928741258408503No Hit
CCACTGCTTCCCATGTACTCTGCGT71970.19025700160041115No Hit
CCATGTACTCTGCGTTGATACCACT66900.176854153217556No Hit
ATGGGAAGCAGTGGTATCAACGCAG63270.16725803100261236No Hit
GTACTTTTTTTTTTTTTTTTTTTTT63190.16704654621550616No Hit
ACGCAGAGTACTTTTTTTTTTTTTT62420.16501100513960903No Hit
GTACATGGTAAGCAGTGGTATCAAC55390.14642677947265212No Hit
TTCCCATGTACTCTGCGTTGATACC53700.14195916334503372No Hit
TGATACCACTGCTTCCCATGTACTC49610.13114700360422948No Hit
ACCATGTACTCTGCGTTGATACCAC49030.12961373889770958No Hit
ATCAACGCAGAGTACATGGGAAGCA46500.12292553250547614No Hit
GAGTACTTTTTTTTTTTTTTTTTTT40160.10616536312731013No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCTA600.005811227511.1004335
ATAACGG700.001473188510.8738955
CTAGCAC2550.010.4474663
CGGACTG750.00261540710.1489685
GTATTAG3000.09.8363731
GTTCTAG2401.8189894E-129.5190711
GTCCTAA2600.09.519071
TAGACTG3000.09.5146585
TGGACCG1106.727228E-59.5146575
TACGCAG1106.727228E-59.5146575
ACCGCCC800.00453947539.4945348
GACCACG1106.884862E-59.4945347
AGGACTG6550.09.1515035
CTAATAC3550.09.112633
TAGGACT3350.09.0886284
TCTACGC1054.4264918E-49.0615793
TGGACAG6200.09.0542715
AGCGATT1054.5235583E-49.04193612
GTGTAGG3700.09.0045261
TTAGGAC2651.8189894E-128.9760923
Produced by FastQC (version 0.11.5)