FastQCFastQC Report
Wed 25 May 2016
SRR1294707_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294707_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3320942
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC1348244.059811944924061No Hit
CCCATGTACTCTGCGTTGATACCAC1267093.8154535670903016No Hit
GAGTACATGGGAAGCAGTGGTATCA631951.902923929415208No Hit
CATGTACTCTGCGTTGATACCACTG502751.513877688920794No Hit
GCGTTGATACCACTGCTTCCCATGT400221.2051399873891204No Hit
GCTTCCCATGTACTCTGCGTTGATA370331.1151354043521386No Hit
ACGCAGAGTACATGGGAAGCAGTGG345671.0408793649512698No Hit
GTATCAACGCAGAGTACATGGGAAG326710.9837871302780958No Hit
ACTCTGCGTTGATACCACTGCTTCC270520.8145881499887683No Hit
TATCAACGCAGAGTACATGGGAAGC265400.7991708376719617No Hit
GGTATCAACGCAGAGTACATGGGAA221530.6670697651449499No Hit
CAGTGGTATCAACGCAGAGTACATG186510.5616177578530429No Hit
GTGGTATCAACGCAGAGTACATGGG170570.5136193284917352No Hit
GCAGTGGTATCAACGCAGAGTACAT151260.4554731759844044No Hit
ATACCACTGCTTCCCATGTACTCTG136500.4110279553211107No Hit
CATGGGAAGCAGTGGTATCAACGCA129800.3908529567815397No Hit
GTACTCTGCGTTGATACCACTGCTT126600.3812171365835356No Hit
GTACATGGTAAGCAGTGGTATCAAC126160.37989221130631007No Hit
ACATGGGAAGCAGTGGTATCAACGC124440.37471295794988285No Hit
CTGCTTCCCATGTACTCTGCGTTGA123420.3716415402617691No Hit
GGGAAGCAGTGGTATCAACGCAGAG122470.36878090614048664No Hit
AAGCAGTGGTATCAACGCAGAGTAC109900.33093019992520195No Hit
TACCACTGCTTCCCATGTACTCTGC104680.3152117682272078No Hit
GTTGATACCACTGCTTCCCATGTAC104400.31436863395988246No Hit
GATACCACTGCTTCCCATGTACTCT103610.3119897908485002No Hit
CTTCCCATGTACTCTGCGTTGATAC103510.3116886714673126No Hit
GCAGAGTACATGGGAAGCAGTGGTA103500.31165855952919386No Hit
CCACTGCTTCCCATGTACTCTGCGT99800.3005171424252516No Hit
GTACATGGGTAAGCAGTGGTATCAA92150.27748150976439817No Hit
CCATGTACTCTGCGTTGATACCACT88120.2653463987025368No Hit
ACCATGTACTCTGCGTTGATACCAC85400.2571559515342334No Hit
ATGGGAAGCAGTGGTATCAACGCAG85350.2570053918436396No Hit
TTCCCATGTACTCTGCGTTGATACC78470.2362883784179308No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA78380.23601737097486195No Hit
TGATACCACTGCTTCCCATGTACTC69010.20780248495758133No Hit
GGTAAGCAGTGGTATCAACGCAGAG61830.1861821133883097No Hit
CCCCATGTACTCTGCGTTGATACCA59630.1795574870021819No Hit
ATCAACGCAGAGTACATGGGAAGCA51160.15405267541558992No Hit
GCTTACCATGTACTCTGCGTTGATA48780.14688603414332438No Hit
GAGTACATGGTAAGCAGTGGTATCA41570.1251753267596965No Hit
GGAAGCAGTGGTATCAACGCAGAGT41130.12385040148247095No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA39120.11779790192059963No Hit
AAAAAGTACTCTGCGTTGATACCAC38840.11695476765327427No Hit
TCCATGTACTCTGCGTTGATACCAC33380.10051364944042986No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCCG751.4840933E-512.6630825
TCATCCG604.1086826E-412.66136610
GTACAAA8200.011.701611
GTGCGCC658.0568914E-411.68723911
CGCAAAA3400.011.454022
CGGATTC600.005849620311.09055218
GTAGGAC1803.6379788E-1211.08153253
GTTAGAC1355.7083525E-810.5538413
CCTAGAC2600.010.2291063
GCCCCGA750.002658471510.12894111
GTGTAGG2450.010.0819681
GAACAAA15200.010.0628361
GATACGA1054.0787418E-59.95886318
TATACTG5400.09.8490645
ATACGAC1106.659189E-59.52341519
CGGTATC1201.6883196E-59.50633116
GGTATCG1304.2063E-69.506330517
GTCCAGG3400.09.5003161
CCAGTAC2303.6379788E-129.4984563
CTAGGAC1502.6572343E-79.4984563
Produced by FastQC (version 0.11.5)