##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294707_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3320942
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.008510235951125	33.0	33.0	33.0	27.0	33.0
2	31.004675781751082	33.0	33.0	33.0	27.0	33.0
3	31.10544989945624	33.0	33.0	33.0	27.0	33.0
4	34.48050974693325	37.0	37.0	37.0	27.0	37.0
5	34.91626984150882	37.0	37.0	37.0	33.0	37.0
6	34.93183711127746	37.0	37.0	37.0	33.0	37.0
7	35.021195492122416	37.0	37.0	37.0	33.0	37.0
8	34.865712800765564	37.0	37.0	37.0	33.0	37.0
9	34.578577403640296	37.0	37.0	37.0	27.0	37.0
10	34.72957160950116	37.0	37.0	37.0	27.0	37.0
11	34.96658628786651	37.0	37.0	37.0	33.0	37.0
12	34.91680613512672	37.0	37.0	37.0	33.0	37.0
13	34.74452881140351	37.0	37.0	37.0	27.0	37.0
14	36.07375588010871	40.0	37.0	40.0	27.0	40.0
15	36.267418100045106	40.0	37.0	40.0	27.0	40.0
16	36.256421220244135	40.0	37.0	40.0	27.0	40.0
17	36.264367760713675	40.0	37.0	40.0	27.0	40.0
18	36.24902000697392	40.0	37.0	40.0	27.0	40.0
19	36.24145137132778	40.0	37.0	40.0	27.0	40.0
20	36.16668734353084	40.0	37.0	40.0	27.0	40.0
21	36.09389444320316	40.0	37.0	40.0	27.0	40.0
22	36.02894510051666	40.0	37.0	40.0	27.0	40.0
23	35.71047642506253	40.0	37.0	40.0	27.0	40.0
24	36.06109200341349	40.0	37.0	40.0	27.0	40.0
25	35.02474568962662	37.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.6366381291240621
1101	2	0.6554406702320854
1101	3	0.5590463870947957
1101	4	0.7379268772864123
1101	5	0.6893965496516401
1101	6	0.548504199619039
1101	7	0.48435085793918375
1101	8	0.5823200561226116
1101	9	0.8451247043381898
1101	10	0.7866894801572357
1101	11	0.6160885612242666
1101	12	0.6007000423511926
1101	13	0.6071984561999457
1101	14	1.2679094663142436
1101	15	1.0918472582799765
1101	16	1.0602231550321761
1101	17	1.0773945658270065
1101	18	1.0959371237061077
1101	19	1.0282630468456873
1101	20	1.1027542298394621
1101	21	1.0237242661836419
1101	22	1.1676940530408189
1101	23	1.4987204858360599
1101	24	1.2694361368686984
1101	25	1.145579024164178
1102	1	0.42424044539309236
1102	2	0.547655487322956
1102	3	0.3682099352425361
1102	4	0.48271413145565845
1102	5	0.3864823390003238
1102	6	0.37149809407556944
1102	7	0.43073026752941956
1102	8	0.5424859805616435
1102	9	0.716709260815243
1102	10	0.6784391023345222
1102	11	0.5421041875870145
1102	12	0.48152731195984444
1102	13	0.5332995986356366
1102	14	1.1869297705671897
1102	15	1.0158434619388288
1102	16	0.6140750682083151
1102	17	0.8747323413446324
1102	18	0.9397783339553385
1102	19	0.7784966730000136
1102	20	0.8609179510767433
1102	21	0.8658791728248687
1102	22	0.9765853094248911
1102	23	1.3036390018101613
1102	24	0.9964714261138425
1102	25	1.1000984725855076
1103	1	0.4229725333018983
1103	2	0.5756798230833624
1103	3	0.3831753210843303
1103	4	0.3436281982173739
1103	5	0.3539134071508201
1103	6	0.33852640644539633
1103	7	0.3458058072968271
1103	8	0.5732447513703249
1103	9	0.7147240305768534
1103	10	0.6914561515972224
1103	11	0.5790624107883815
1103	12	0.5247146781654308
1103	13	0.5015366175576972
1103	14	1.1286987512568771
1103	15	0.8734006449146889
1103	16	0.5683474857180428
1103	17	0.6744877890887224
1103	18	0.8090681397980646
1103	19	0.8543025139690101
1103	20	0.9877873396170429
1103	21	0.9790306204178663
1103	22	0.8599003276916477
1103	23	1.3196652560408566
1103	24	1.0569085984081141
1103	25	0.9355440557556705
1104	1	0.43412989377915423
1104	2	0.5462212936814588
1104	3	0.39240803624586107
1104	4	0.487312626143904
1104	5	0.39186391303792334
1104	6	0.3684300040064912
1104	7	0.32025809265426375
1104	8	0.5454951739106093
1104	9	0.6425305970297117
1104	10	0.605481017817084
1104	11	0.4408343326965678
1104	12	0.3833260671978209
1104	13	0.44466484541029416
1104	14	1.03361445728995
1104	15	0.8088090093077867
1104	16	0.7220037628723759
1104	17	0.8754556138700309
1104	18	0.7829289574414773
1104	19	0.9003701087590272
1104	20	0.8970003180748165
1104	21	0.9882479651040654
1104	22	1.0259363403775197
1104	23	1.3152839104942444
1104	24	1.1018944748837072
1104	25	1.0073899645447355
1105	1	0.21062072281131705
1105	2	0.25470115837687857
1105	3	0.230144600559278
1105	4	0.32526497793581655
1105	5	0.25252060682382194
1105	6	0.15729653653499298
1105	7	0.16821248567907787
1105	8	0.25560802492976364
1105	9	-1.5696526473641939
1105	10	-0.2290828873530657
1105	11	0.024979785121260534
1105	12	0.1315429044532337
1105	13	0.19975480546045077
1105	14	-1.6550042112429892
1105	15	-0.2384367842944073
1105	16	-0.013659492019293396
1105	17	0.29648322883518574
1105	18	0.20029984946025792
1105	19	0.33230957162317054
1105	20	0.43836736091154904
1105	21	0.5419440546850325
1105	22	0.5981755728739486
1105	23	0.8111460397917725
1105	24	0.5536659734402676
1105	25	0.349013619518054
1106	1	0.23667892004399604
1106	2	0.2547813107490171
1106	3	0.24638909008369225
1106	4	-0.93388953848509
1106	5	-0.11458097597894579
1106	6	0.12184315918671729
1106	7	0.16758531402492594
1106	8	0.2702498801679667
1106	9	0.5317661322539706
1106	10	0.47109311734038783
1106	11	0.3462920529789244
1106	12	0.20614617086897624
1106	13	0.2709912590898256
1106	14	0.7574037064892778
1106	15	0.517799194204926
1106	16	0.36042278397754757
1106	17	0.12015668641846844
1106	18	0.33457118589777224
1106	19	0.3446410553923158
1106	20	0.3895293637292738
1106	21	0.5636731975091251
1106	22	0.640278705013813
1106	23	0.8450302485349894
1106	24	0.40838194896487323
1106	25	0.4145964388157708
1107	1	0.33269112792395106
1107	2	0.3358808176571557
1107	3	0.31503140794701423
1107	4	0.5431294783009832
1107	5	0.46851185032979714
1107	6	0.2637987842831251
1107	7	0.2887346827510484
1107	8	0.32855716371285126
1107	9	0.3985708287605192
1107	10	0.5269232642299571
1107	11	0.24648259086280433
1107	12	0.3094122737229128
1107	13	0.3232144493523563
1107	14	0.8314580210790936
1107	15	0.6106009949200839
1107	16	0.582329594853924
1107	17	0.6588233825588361
1107	18	0.5691046275790015
1107	19	0.5966691906054962
1107	20	0.5687717389702129
1107	21	0.6979800600926254
1107	22	0.6892788977141677
1107	23	0.901581844415908
1107	24	0.810004284137591
1107	25	0.8153081807068574
1108	1	0.3576965599038999
1108	2	0.31150570245550924
1108	3	0.39615993070870203
1108	4	0.4651164763065694
1108	5	0.5528648035935149
1108	6	0.3301361134346408
1108	7	0.29542369444765626
1108	8	0.3430229337749111
1108	9	0.13904424348455535
1108	10	0.4339578425980122
1108	11	0.25889996646326097
1108	12	0.38444693889459103
1108	13	0.3773267395660511
1108	14	-0.9534444873745755
1108	15	-0.00913740200606128
1108	16	0.22738322445655257
1108	17	0.447329592124575
1108	18	0.5210125829601395
1108	19	0.48128151021896315
1108	20	0.4960814019875528
1108	21	0.6059394932649553
1108	22	0.7692305290055046
1108	23	1.0607195872713362
1108	24	0.9440114321099458
1108	25	0.7944993077915967
1109	1	0.35276522480426564
1109	2	0.265094315800507
1109	3	0.253372561480699
1109	4	0.3709948705997803
1109	5	0.4367935448321063
1109	6	0.2578684088334242
1109	7	0.2716835018904149
1109	8	0.24182724136908718
1109	9	0.3865759791477288
1109	10	0.4233342772313975
1109	11	0.23026625680306267
1109	12	0.21513189084305395
1109	13	0.26761175705595974
1109	14	0.6233537693401274
1109	15	0.38720566354854213
1109	16	0.33944621446294576
1109	17	0.4090231068867638
1109	18	0.4693085786414244
1109	19	0.41895262385290266
1109	20	0.5489831468821222
1109	21	0.5056739626499294
1109	22	0.5401104521113496
1109	23	0.9416694846433913
1109	24	0.7262983456201155
1109	25	0.5982481904416943
1110	1	0.18718456782364967
1110	2	0.09340741164451671
1110	3	0.15237879311619196
1110	4	0.3254862530933238
1110	5	0.247469301617258
1110	6	0.04825171643328474
1110	7	0.07405383297545853
1110	8	0.13148480793752526
1110	9	0.21758574361158622
1110	10	0.29296435674142174
1110	11	0.09106184176559395
1110	12	0.07886251767059349
1110	13	0.04362537797691601
1110	14	0.4494741022072688
1110	15	0.3388553147565929
1110	16	0.1819757366757102
1110	17	0.22370957906252897
1110	18	0.3283986258456011
1110	19	0.2385792104535298
1110	20	0.4348243178764335
1110	21	0.4590366364221765
1110	22	0.5055558123158477
1110	23	0.5777567275403044
1110	24	0.533482404837045
1110	25	0.4053215458160011
1111	1	0.13020464536596066
1111	2	0.07703845107810992
1111	3	0.2545951672581346
1111	4	0.4080512946473789
1111	5	0.2282725927363316
1111	6	0.19763530017179676
1111	7	0.1257319710908078
1111	8	0.23680673277863917
1111	9	0.3041725474899266
1111	10	0.2542327568838232
1111	11	0.1725626822065962
1111	12	0.14451539018485704
1111	13	0.03997487775155406
1111	14	-1.737085555175831
1111	15	-0.5038822804511369
1111	16	-0.04279619933600287
1111	17	0.09935850426188608
1111	18	0.10461414533954638
1111	19	0.08927745619658367
1111	20	0.34336771967632274
1111	21	0.32804386304484723
1111	22	0.144134909876648
1111	23	-1.8699287447251365
1111	24	-0.2592784090508147
1111	25	0.05530119639088582
1112	1	-0.3707476614704497
1112	2	-0.450006901508349
1112	3	-0.19109614584010615
1112	4	-0.09035443626596162
1112	5	-0.6529342316670679
1112	6	-0.83557668685755
1112	7	-0.8870217103634346
1112	8	-0.6707051276355003
1112	9	-0.6573481571390403
1112	10	-0.7542110393750221
1112	11	-0.687563397132827
1112	12	-0.539465093516462
1112	13	-0.8089675540682322
1112	14	-0.5211617946557539
1112	15	-0.7652007023442025
1112	16	-0.7740361047219722
1112	17	-0.7068563446696388
1112	18	-0.9220306540391761
1112	19	-0.7401111797790421
1112	20	-0.8855019878377064
1112	21	-1.7040107608336683
1112	22	-1.5968935734282894
1112	23	-1.2408597973331936
1112	24	-1.4706685804579678
1112	25	-1.8365260775261163
1113	1	-0.10926531421938535
1113	2	-0.2331365700329222
1113	3	-0.13828743180818037
1113	4	0.06854977242736027
1113	5	-0.04232135597988673
1113	6	-0.17803158451035017
1113	7	-0.08130922498556004
1113	8	-0.12910345637459386
1113	9	0.024818928526812556
1113	10	-0.16931286131341494
1113	11	-0.1602259811493454
1113	12	-0.1534372889250477
1113	13	-0.17732910206337493
1113	14	-0.1286868775349106
1113	15	-0.26980354468348366
1113	16	-0.18238674454364912
1113	17	-0.14005068317323577
1113	18	-0.19345706125144346
1113	19	-0.3863694035089793
1113	20	-0.6727385091037092
1113	21	-0.6384471174697168
1113	22	-0.16789756692132585
1113	23	-0.44961048637116363
1113	24	-0.16138644175475036
1113	25	-1.2189934846481805
1114	1	-0.9857805890914939
1114	2	-0.7063339068126879
1114	3	-0.5993780221552463
1114	4	-1.549932191524043
1114	5	-0.8199391248089256
1114	6	-0.6575046501785806
1114	7	-0.6586184966472715
1114	8	-0.5048245670545342
1114	9	-0.5568492483041183
1114	10	-0.6970050116076933
1114	11	-0.6438373991114972
1114	12	-0.7366664946456041
1114	13	-0.697751602950774
1114	14	-0.6538541786894783
1114	15	-1.430546703890471
1114	16	-1.00818770302633
1114	17	-1.0006489039544206
1114	18	-0.9883003927349776
1114	19	-0.947851954077592
1114	20	-2.1686056939714433
1114	21	-6.9157764388074945
1114	22	-7.854266227410452
1114	23	-8.108986385153909
1114	24	-6.135061970391114
1114	25	-7.501187910748094
1115	1	-1.0252873331004686
1115	2	-1.020559378733239
1115	3	-0.9019476957711134
1115	4	-0.7711038936238097
1115	5	-0.8692020054219185
1115	6	-0.9681909229775059
1115	7	-1.170335832549526
1115	8	-0.8984837933576699
1115	9	-2.8541278694210632
1115	10	-1.713520443177167
1115	11	-1.204218575324603
1115	12	-1.0086865561648324
1115	13	-1.3891736161171906
1115	14	-1.3057795510345898
1115	15	-2.006694954129898
1115	16	-1.423798072675723
1115	17	-3.168539937007985
1115	18	-2.8672798841825013
1115	19	-2.1425661631088673
1115	20	-1.8710449052562552
1115	21	-1.3217414788915676
1115	22	-0.8966309270302162
1115	23	-0.4375033408614044
1115	24	-1.453819246818398
1115	25	-1.2305555942196875
1116	1	-1.6410754054268217
1116	2	-0.9635208171544711
1116	3	-0.8429105888145116
1116	4	-0.6206770856847328
1116	5	-0.5539362698127519
1116	6	-0.6611394947227964
1116	7	-1.039967115555406
1116	8	-1.9330624397484684
1116	9	-1.1571526152429854
1116	10	-2.533607609119173
1116	11	-1.2914133373427674
1116	12	-1.02669181677323
1116	13	-1.0750616539601836
1116	14	-1.489527057130573
1116	15	-1.6213745128367947
1116	16	-1.1236484166269705
1116	17	-1.0521503349999364
1116	18	-1.6316042186264283
1116	19	-1.1894506191511027
1116	20	-1.394645274286617
1116	21	-0.818657100136619
1116	22	-0.4302108375425746
1116	23	-0.4470627274990733
1116	24	-0.50862655568082
1116	25	-0.48504847976987975
1201	1	0.37481566268446187
1201	2	0.3700939498181377
1201	3	0.2826810987868953
1201	4	0.4387519023804316
1201	5	0.48119153531341397
1201	6	0.3200734880390925
1201	7	0.3833055278901156
1201	8	0.4060706849719864
1201	9	0.6803627003628279
1201	10	0.5727120678469788
1201	11	0.40015865798585537
1201	12	0.428123714088521
1201	13	0.42677217775466403
1201	14	1.2147438569633948
1201	15	0.8828151737382015
1201	16	0.8930317004678585
1201	17	0.7502242216769375
1201	18	0.6323414470066169
1201	19	0.7107168399068513
1201	20	0.8390692747335393
1201	21	0.8404083657012009
1201	22	0.8917356499582709
1201	23	0.5910802923782867
1201	24	0.6593602092338315
1201	25	0.6962563805762301
1202	1	-0.01754164466134256
1202	2	0.29109880413013656
1202	3	0.247635075501762
1202	4	0.2989333313555136
1202	5	0.24665244783854945
1202	6	0.09401818358223579
1202	7	0.1864371167605725
1202	8	0.3222506992907981
1202	9	0.453383247284485
1202	10	0.5442482556832786
1202	11	0.33594543265480326
1202	12	0.3432958810205591
1202	13	0.4032344750312973
1202	14	0.6276798084091055
1202	15	0.7162162436369286
1202	16	0.7026440579024396
1202	17	0.5095298127501309
1202	18	0.5008864339893861
1202	19	0.4710157371128716
1202	20	0.7536730777128753
1202	21	0.7629256835340215
1202	22	0.7688569473427975
1202	23	1.016404266972124
1202	24	0.6487010805916427
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2211	9	0.3630907801460239
2211	10	0.3162650447729263
2211	11	0.1349328562542027
2211	12	0.13029626734763866
2211	13	0.1014076575530325
2211	14	0.31464229109102604
2211	15	0.1275096388868917
2211	16	0.16132335091553784
2211	17	0.13839514347595383
2211	18	0.16263338573054398
2211	19	0.14823198587621533
2211	20	0.2114272969900597
2211	21	0.29291387652197187
2211	22	0.2978947013023472
2211	23	0.45167523019024713
2211	24	0.3601421717123543
2211	25	0.4155153542270753
2212	1	-0.2617795275623678
2212	2	-0.2757184544916953
2212	3	-0.20928392347995484
2212	4	-0.39713872791065086
2212	5	-0.25346940587257194
2212	6	-0.49587864803661574
2212	7	-0.40414906339949397
2212	8	-0.28568388748683304
2212	9	-0.15888460436626417
2212	10	-0.5070164356497173
2212	11	-0.42413472529747764
2212	12	-0.57285304928687
2212	13	-0.4791758935520747
2212	14	-0.376011708631637
2212	15	-0.6406127448664733
2212	16	-0.3843122604453555
2212	17	-0.3706143517415086
2212	18	-0.37627066291724987
2212	19	-0.3958195564326985
2212	20	-0.431102451090986
2212	21	-0.34824753647132667
2212	22	-0.4322077081665583
2212	23	0.1055483713473464
2212	24	-0.12456435060409632
2212	25	-0.8770384732149878
2213	1	-0.028235957122713984
2213	2	-1.1509846161317014
2213	3	-0.22445928763764655
2213	4	0.12080144902579804
2213	5	0.05433182920872781
2213	6	0.06239722766919442
2213	7	0.06349567067579898
2213	8	0.19580903928859783
2213	9	0.19627142538760722
2213	10	-0.31863425556589675
2213	11	-0.06198500021778841
2213	12	0.10156106061191394
2213	13	-0.24416313141698254
2213	14	0.13754894186704547
2213	15	-0.015870282853661877
2213	16	-0.02494227838778329
2213	17	0.1297051415291719
2213	18	0.20190532824098284
2213	19	0.08474518655544472
2213	20	0.2674559703241002
2213	21	-0.3753331930370507
2213	22	-0.8190958883092705
2213	23	-0.07548394783861312
2213	24	0.022614155699095306
2213	25	-0.5390397495156023
2214	1	-0.22353970962904768
2214	2	-0.47695822287557377
2214	3	-0.38463665358550614
2214	4	-0.5898147006969907
2214	5	-0.23766877631935301
2214	6	-0.24258261212978027
2214	7	-0.4726958676668005
2214	8	-0.1151852480319917
2214	9	-0.09455366638152185
2214	10	-1.881427106948344
2214	11	-0.7634395810053718
2214	12	-0.4211172359120994
2214	13	-0.3061014400067421
2214	14	-0.7512684022798126
2214	15	-0.518896118137576
2214	16	-0.817581340078199
2214	17	-0.7108531456529832
2214	18	-0.5465894103623512
2214	19	-1.2913683302904104
2214	20	-2.4060552758910347
2214	21	-1.7754587492352627
2214	22	-1.9647785191395641
2214	23	-2.3664763565780333
2214	24	-1.5410370977216274
2214	25	-1.9822960477421674
2215	1	-0.2750420071442221
2215	2	-0.32425743174168176
2215	3	-0.5739657172527863
2215	4	-0.3290020216917
2215	5	-0.33869238424066594
2215	6	-0.34028009612570287
2215	7	-0.3013586805911217
2215	8	-0.2464613590899205
2215	9	-0.18535939294597625
2215	10	-0.49733555826205134
2215	11	-0.6126661147980741
2215	12	-0.4034383767772596
2215	13	-0.31870445850091755
2215	14	-0.261100618045063
2215	15	-0.5821841068073326
2215	16	-0.5039837012715367
2215	17	-0.37734182200109956
2215	18	-0.3533076146953391
2215	19	-2.33056715748873
2215	20	-2.456605911497732
2215	21	-3.329575376573544
2215	22	-2.6004447555535037
2215	23	-3.2106005410418206
2215	24	-2.157409880950482
2215	25	-1.5348329005504553
2216	1	-1.4995392134142236
2216	2	-1.0372431978187748
2216	3	-0.8196097000984714
2216	4	-0.8630109495589764
2216	5	-1.8817010241314946
2216	6	-1.55211813865062
2216	7	-1.1711784652898913
2216	8	-5.107296816398907
2216	9	-3.7342442907920095
2216	10	-1.9749423276204041
2216	11	-1.0073647940496713
2216	12	-1.176701959467131
2216	13	-2.1519531763485276
2216	14	-3.5020475825821364
2216	15	-2.013554375808404
2216	16	-2.841042356457386
2216	17	-2.5956246817864397
2216	18	-2.6700300245204716
2216	19	-1.5606825183435262
2216	20	-0.8856563868381073
2216	21	-0.8863163704388128
2216	22	-0.725755734267139
2216	23	-1.1728087856521228
2216	24	-0.669218408877029
2216	25	-0.47568839373102634
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	25.0
8	182.0
9	505.0
10	1066.0
11	2111.0
12	3701.0
13	5844.0
14	9123.0
15	13931.0
16	18818.0
17	20824.0
18	20711.0
19	20863.0
20	21119.0
21	22475.0
22	23113.0
23	23809.0
24	25149.0
25	26558.0
26	27623.0
27	30008.0
28	33596.0
29	39798.0
30	47992.0
31	58491.0
32	79990.0
33	115555.0
34	170828.0
35	275155.0
36	491678.0
37	1690299.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.365466197418975	19.505777843107253	11.02600541458178	24.10275054489199
2	12.382859450615078	23.394530364161533	36.60787290500732	27.61473728021607
3	21.23976911133767	29.25602709463092	27.144236141334222	22.35996765269719
4	12.908583052677042	19.737822548923344	33.37506784242126	33.978526555978355
5	11.221772988810393	41.02650905481076	34.681003450369616	13.070714506009235
6	32.03976234141288	33.34769764479497	18.47912958897592	16.13341042481623
7	23.83912246426911	34.389491396401496	22.78487149406071	18.986514645268684
8	26.45050439169016	36.481667339670715	21.49162388498888	15.576204383650246
9	26.520656794493807	18.14571095875025	20.52610769856639	34.80752454818955
10	15.979219086690122	32.781309584176974	32.08966639485542	19.149804934277487
11	28.600409405362498	28.49595021129725	19.316158142005484	23.587482241334765
12	22.792196431620308	32.13095521653677	28.480549346545853	16.596299005297073
13	28.23874536756405	23.08209548260896	22.753658145122508	25.925501004704483
14	20.740793827260124	27.50845848926383	25.879333583063023	25.871414100413016
15	30.797105813426867	25.610925349547585	21.314272430307295	22.27769640671825
16	18.683799171359475	29.74680361508657	31.307121857855535	20.262275355698417
17	25.171559113930186	30.508990573847132	27.26345258632761	17.055997725895075
18	25.265147853748537	26.655440736695628	28.622417591947684	19.456993817608147
19	22.553253891755055	31.8705206461682	29.15266833306684	16.423557129009907
20	23.146370701468953	28.38092691477179	27.514693921674372	20.95800846208488
21	18.77611934001229	30.702767146959648	30.319304269836834	20.201809243191228
22	21.146535281845214	29.95184572308714	22.848313877044614	26.05330511802303
23	17.39448307079104	31.289088645581913	27.42893185854389	23.887496425083153
24	20.279544028319965	35.78078645960683	24.560993559592738	19.378675952480467
25	21.762776276963724	27.443428733196573	22.198553926842834	28.59524106299687
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	10374.0
1	10374.0
2	12759.0
3	15144.0
4	15144.0
5	15144.0
6	18377.5
7	21611.0
8	21611.0
9	21611.0
10	23148.0
11	24685.0
12	24685.0
13	24685.0
14	29377.0
15	34069.0
16	34069.0
17	34069.0
18	44868.0
19	55667.0
20	55667.0
21	55667.0
22	72560.0
23	89453.0
24	89453.0
25	89453.0
26	110781.0
27	132109.0
28	132109.0
29	132109.0
30	154340.0
31	176571.0
32	176571.0
33	176571.0
34	195702.0
35	214833.0
36	214833.0
37	214833.0
38	233098.5
39	251364.0
40	251364.0
41	251364.0
42	291321.5
43	331279.0
44	331279.0
45	331279.0
46	579659.0
47	828039.0
48	828039.0
49	828039.0
50	704327.5
51	580616.0
52	580616.0
53	580616.0
54	418054.5
55	255493.0
56	255493.0
57	255493.0
58	194410.5
59	133328.0
60	133328.0
61	133328.0
62	109074.5
63	84821.0
64	84821.0
65	84821.0
66	65604.5
67	46388.0
68	46388.0
69	46388.0
70	33906.5
71	21425.0
72	21425.0
73	21425.0
74	15204.0
75	8983.0
76	8983.0
77	8983.0
78	6117.5
79	3252.0
80	3252.0
81	3252.0
82	2138.5
83	1025.0
84	1025.0
85	1025.0
86	660.0
87	295.0
88	295.0
89	295.0
90	188.5
91	82.0
92	82.0
93	82.0
94	48.0
95	14.0
96	14.0
97	14.0
98	18.0
99	22.0
100	22.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.030653953004900418
2	0.014002051225224651
3	0.021891379012340475
4	0.022523729712834492
5	0.02162037156927161
6	0.00638373088117769
7	1.8067162871257612E-4
8	3.3123131930638957E-4
9	7.527984529690673E-4
10	3.011193811876269E-4
11	8.431342673253554E-4
12	0.001535708844056897
13	0.0019572759777195746
14	5.420148861377284E-4
15	0.0023487311732634897
16	0.0024390669876197778
17	0.0022583953589072015
18	0.0017766043490069986
19	3.3123131930638957E-4
20	3.3123131930638957E-4
21	0.08307883726966625
22	0.07976652407660235
23	0.08079032997264028
24	0.0010238058960379314
25	0.22749569248725213
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3320942.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.54786315250377
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.67264516467655	49.4599904011831
2	6.9312663495025895	7.561715366124433
3	1.1724142493448642	1.9185807609392729
4	0.3635789957850017	0.7932982922882008
5	0.2015652177711528	0.5497475957642731
6	0.11931525632550771	0.39050353644298236
7	0.08110594225974037	0.3096909087467433
8	0.06645065446555946	0.28997929649453213
9	0.050757405083582494	0.2491837187827912
>10	0.2820493533823654	2.8914822140332794
>50	0.02871827843396428	1.0735146684075125
>100	0.021219062532234375	2.317244622242891
>500	0.00341986350097081	1.298575943370856
>1k	0.003363800164885322	3.9022110767001386
>5k	6.166966968956423E-4	2.5972742121467918
>10k+	0.001513710074198395	24.39700738633221
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	134824	4.059811944924061	No Hit
CCCATGTACTCTGCGTTGATACCAC	126709	3.8154535670903016	No Hit
GAGTACATGGGAAGCAGTGGTATCA	63195	1.902923929415208	No Hit
CATGTACTCTGCGTTGATACCACTG	50275	1.513877688920794	No Hit
GCGTTGATACCACTGCTTCCCATGT	40022	1.2051399873891204	No Hit
GCTTCCCATGTACTCTGCGTTGATA	37033	1.1151354043521386	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	34567	1.0408793649512698	No Hit
GTATCAACGCAGAGTACATGGGAAG	32671	0.9837871302780958	No Hit
ACTCTGCGTTGATACCACTGCTTCC	27052	0.8145881499887683	No Hit
TATCAACGCAGAGTACATGGGAAGC	26540	0.7991708376719617	No Hit
GGTATCAACGCAGAGTACATGGGAA	22153	0.6670697651449499	No Hit
CAGTGGTATCAACGCAGAGTACATG	18651	0.5616177578530429	No Hit
GTGGTATCAACGCAGAGTACATGGG	17057	0.5136193284917352	No Hit
GCAGTGGTATCAACGCAGAGTACAT	15126	0.4554731759844044	No Hit
ATACCACTGCTTCCCATGTACTCTG	13650	0.4110279553211107	No Hit
CATGGGAAGCAGTGGTATCAACGCA	12980	0.3908529567815397	No Hit
GTACTCTGCGTTGATACCACTGCTT	12660	0.3812171365835356	No Hit
GTACATGGTAAGCAGTGGTATCAAC	12616	0.37989221130631007	No Hit
ACATGGGAAGCAGTGGTATCAACGC	12444	0.37471295794988285	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	12342	0.3716415402617691	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	12247	0.36878090614048664	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	10990	0.33093019992520195	No Hit
TACCACTGCTTCCCATGTACTCTGC	10468	0.3152117682272078	No Hit
GTTGATACCACTGCTTCCCATGTAC	10440	0.31436863395988246	No Hit
GATACCACTGCTTCCCATGTACTCT	10361	0.3119897908485002	No Hit
CTTCCCATGTACTCTGCGTTGATAC	10351	0.3116886714673126	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	10350	0.31165855952919386	No Hit
CCACTGCTTCCCATGTACTCTGCGT	9980	0.3005171424252516	No Hit
GTACATGGGTAAGCAGTGGTATCAA	9215	0.27748150976439817	No Hit
CCATGTACTCTGCGTTGATACCACT	8812	0.2653463987025368	No Hit
ACCATGTACTCTGCGTTGATACCAC	8540	0.2571559515342334	No Hit
ATGGGAAGCAGTGGTATCAACGCAG	8535	0.2570053918436396	No Hit
TTCCCATGTACTCTGCGTTGATACC	7847	0.2362883784179308	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	7838	0.23601737097486195	No Hit
TGATACCACTGCTTCCCATGTACTC	6901	0.20780248495758133	No Hit
GGTAAGCAGTGGTATCAACGCAGAG	6183	0.1861821133883097	No Hit
CCCCATGTACTCTGCGTTGATACCA	5963	0.1795574870021819	No Hit
ATCAACGCAGAGTACATGGGAAGCA	5116	0.15405267541558992	No Hit
GCTTACCATGTACTCTGCGTTGATA	4878	0.14688603414332438	No Hit
GAGTACATGGTAAGCAGTGGTATCA	4157	0.1251753267596965	No Hit
GGAAGCAGTGGTATCAACGCAGAGT	4113	0.12385040148247095	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	3912	0.11779790192059963	No Hit
AAAAAGTACTCTGCGTTGATACCAC	3884	0.11695476765327427	No Hit
TCCATGTACTCTGCGTTGATACCAC	3338	0.10051364944042986	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	3.011193811876269E-5	0.0	0.0	0.0	0.0
10	3.011193811876269E-5	0.0	0.0	3.011193811876269E-5	0.0
11	3.011193811876269E-5	0.0	0.0	9.033581435628806E-5	0.0
12	3.011193811876269E-5	0.0	0.0	1.5055969059381345E-4	0.0
13	3.011193811876269E-5	0.0	0.0	2.1078356683133882E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTCCG	75	1.4840933E-5	12.663082	5
TCATCCG	60	4.1086826E-4	12.661366	10
GTACAAA	820	0.0	11.70161	1
GTGCGCC	65	8.0568914E-4	11.687239	11
CGCAAAA	340	0.0	11.45402	2
CGGATTC	60	0.0058496203	11.090552	18
GTAGGAC	180	3.6379788E-12	11.0815325	3
GTTAGAC	135	5.7083525E-8	10.553841	3
CCTAGAC	260	0.0	10.229106	3
GCCCCGA	75	0.0026584715	10.128941	11
GTGTAGG	245	0.0	10.081968	1
GAACAAA	1520	0.0	10.062836	1
GATACGA	105	4.0787418E-5	9.958863	18
TATACTG	540	0.0	9.849064	5
ATACGAC	110	6.659189E-5	9.523415	19
CGGTATC	120	1.6883196E-5	9.506331	16
GGTATCG	130	4.2063E-6	9.5063305	17
GTCCAGG	340	0.0	9.500316	1
CCAGTAC	230	3.6379788E-12	9.498456	3
CTAGGAC	150	2.6572343E-7	9.498456	3
>>END_MODULE