FastQCFastQC Report
Wed 25 May 2016
SRR1294707_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294707_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3320942
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC1397344.2076615610871855No Hit
GTACATGGGAAGCAGTGGTATCAAC1312833.953185572045522No Hit
GAGTACATGGGAAGCAGTGGTATCA692692.0858238415485726No Hit
CATGTACTCTGCGTTGATACCACTG579301.7443845752199225No Hit
GCGTTGATACCACTGCTTCCCATGT427761.2880682649681927No Hit
ACGCAGAGTACATGGGAAGCAGTGG418951.261539647485563No Hit
GCTTCCCATGTACTCTGCGTTGATA418851.2612385281043752No Hit
GTATCAACGCAGAGTACATGGGAAG347151.0453359317928468No Hit
ACTCTGCGTTGATACCACTGCTTCC311850.9390407902336145No Hit
TATCAACGCAGAGTACATGGGAAGC300370.9044722852732748No Hit
GGTATCAACGCAGAGTACATGGGAA242690.7307866262042517No Hit
CAGTGGTATCAACGCAGAGTACATG219430.6607462581400098No Hit
GTGGTATCAACGCAGAGTACATGGG180330.5430085800956476No Hit
GCAGTGGTATCAACGCAGAGTACAT168870.5085002990115455No Hit
CATGGGAAGCAGTGGTATCAACGCA167570.5045857470561064No Hit
ATACCACTGCTTCCCATGTACTCTG154000.4637238470289454No Hit
ACATGGGAAGCAGTGGTATCAACGC152950.4605620935264753No Hit
GGGAAGCAGTGGTATCAACGCAGAG140850.4241266484027725No Hit
CTGCTTCCCATGTACTCTGCGTTGA136040.40964280616764764No Hit
AAGCAGTGGTATCAACGCAGAGTAC128200.3860350466825377No Hit
GTACTCTGCGTTGATACCACTGCTT126860.3820000469746235No Hit
CTTCCCATGTACTCTGCGTTGATAC125850.37895874122462847No Hit
TACCACTGCTTCCCATGTACTCTGC119630.3602291157147581No Hit
GTACATGGTAAGCAGTGGTATCAAC119490.35980754858109537No Hit
GATACCACTGCTTCCCATGTACTCT118910.3580610561702071No Hit
GCAGAGTACATGGGAAGCAGTGGTA118860.3579104964796133No Hit
CCACTGCTTCCCATGTACTCTGCGT113690.342342624472213No Hit
CCATGTACTCTGCGTTGATACCACT107050.3223482975613546No Hit
GTTGATACCACTGCTTCCCATGTAC105780.3185240814202717No Hit
ATGGGAAGCAGTGGTATCAACGCAG102920.3099120671183056No Hit
GTACATGGGTAAGCAGTGGTATCAA96360.29015863571239725No Hit
ACCATGTACTCTGCGTTGATACCAC96060.2892552775688344No Hit
GTATCAACGCAGAGTACTTTTTTTT88950.2678456895663941No Hit
TTCCCATGTACTCTGCGTTGATACC80570.242611885422871No Hit
GGTAAGCAGTGGTATCAACGCAGAG72750.21906434981399858No Hit
TGATACCACTGCTTCCCATGTACTC70720.2129516263758897No Hit
TATCAACGCAGAGTACTTTTTTTTT69370.20888651472985675No Hit
CCCCATGTACTCTGCGTTGATACCA69060.20795304464817513No Hit
GGTATCAACGCAGAGTACTTTTTTT68840.20729058200956238No Hit
ATCAACGCAGAGTACATGGGAAGCA58260.17543215147991142No Hit
GTACTTTTTTTTTTTTTTTTTTTTT55380.16675991330170778No Hit
GCTTACCATGTACTCTGCGTTGATA54770.16492308507646325No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA53030.15968360784379854No Hit
ACGCAGAGTACTTTTTTTTTTTTTT51980.15652185434132845No Hit
GGAAGCAGTGGTATCAACGCAGAGT48780.14688603414332438No Hit
GAGTACATGGTAAGCAGTGGTATCA46450.1398699525616527No Hit
AAAAAGTACTCTGCGTTGATACCAC41780.1258076774601905No Hit
GAGTACTTTTTTTTTTTTTTTTTTT38380.1155696184998112No Hit
TGGGAAGCAGTGGTATCAACGCAGA37110.11174540235872833No Hit
TCCATGTACTCTGCGTTGATACCAC36560.1100892457621964No Hit
ACTCTGCGTTGATACCACTGCTTAC36250.10915577568051474No Hit
CTGCGTTGATACCACTGCTTCCCAT34560.10406685813844384No Hit
ATGGTAAGCAGTGGTATCAACGCAG34050.10253114929438696No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCCG500.001507791113.29106310
ATTAACG855.234892E-511.1955943
CGCAAAA1553.0377123E-1011.0514682
TTAACGC700.001471045310.8757194
CTAGAAC2150.010.6227953
ACGCAAA1803.45608E-1110.5792051
CTAGACT2300.010.3437554
ATACCGT856.630694E-410.0532756
ACGCTGA1054.0082363E-59.9746791
AAAAGTA9150.09.8805632
ATAGGAC1659.951691E-99.8046263
CTAATAC3150.09.6673063
GGTACTC2002.564775E-109.5162545
AAAAAGT9700.09.4231271
TAGGACA2451.8189894E-129.3220454
CTTACTC3000.09.1990463
CGGGTTC1451.7237653E-69.16611218
TCTAGAC2304.0017767E-119.1025043
GAACAAA9000.08.9923241
CCTATAC2651.8189894E-128.9775993
Produced by FastQC (version 0.11.5)