FastQCFastQC Report
Wed 25 May 2016
SRR1294706_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294706_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2445649
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC96410.3942102893751311No Hit
CCCATGTACTCTGCGTTGATACCAC81350.33263154279293555No Hit
GAGTACATGGGAAGCAGTGGTATCA45140.18457268397877208No Hit
GTATCAACGCAGAGTACTTTTTTTT38170.15607309143707868No Hit
CATGTACTCTGCGTTGATACCACTG31780.1299450575286969No Hit
GGTATCAACGCAGAGTACTTTTTTT30010.12270771480290099No Hit
GCGTTGATACCACTGCTTCCCATGT26980.1103183653909453No Hit
ACGCAGAGTACATGGGAAGCAGTGG25990.10627036013753405No Hit
GTATCAACGCAGAGTACATGGGAAG25220.10312191160710307No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGT655.475955E-513.1484676
ACCGCCC752.0826393E-411.3953378
TTAACGC600.005878186311.0831073
TCGGAAC600.005897005611.07834813
GTGTAGG4700.010.714521
CCCGGAG803.773055E-410.6872823
GACCGTG909.5588315E-510.5512397
GTATTAG3500.010.3159741
CAGGACT4300.010.1621684
GACCGGG856.6206726E-410.0547097
TAGTACC1752.577508E-99.7708294
GTATAGG2800.09.5015551
TAGTACT2900.09.4994174
TGTGCCG800.0045223519.498645
TACACCG800.0045223519.498645
GGACCGG800.00453227859.4961156
CCGCCCA800.00453227859.4961159
CTATACA4200.09.2732414
CTAGTAC1856.702976E-99.2430543
CAGGACC3600.09.2355444
Produced by FastQC (version 0.11.5)