FastQCFastQC Report
Wed 25 May 2016
SRR1294706_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294706_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2445649
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT103110.421605880484076No Hit
GTACATGGGAAGCAGTGGTATCAAC97010.39666362589235005No Hit
CCCATGTACTCTGCGTTGATACCAC88010.3598635781340659No Hit
GGTATCAACGCAGAGTACTTTTTTT84570.3457977821020105No Hit
TATCAACGCAGAGTACTTTTTTTTT67470.27587769136127055No Hit
GAGTACATGGGAAGCAGTGGTATCA53480.21867406156811545No Hit
ACGCAGAGTACTTTTTTTTTTTTTT44490.1819149027517849No Hit
CATGTACTCTGCGTTGATACCACTG36150.14781352516244156No Hit
ACGCAGAGTACATGGGAAGCAGTGG33350.1363646214154198No Hit
GCGTTGATACCACTGCTTCCCATGT30480.12462949507472249No Hit
GTATCAACGCAGAGTACATGGGAAG29140.11915037685293352No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGGAC350.002148062716.3112683
TCTACGC500.001482358113.3208693
AATACCG600.005809096611.1007245
TAGACTG2750.011.0718915
GTCGTAT700.001467266110.87885951
TACGCAG700.001472544210.8741795
ACCGTCT700.001499695110.85038958
TAGGACT2850.010.6834044
CTAGGAC2500.010.6566953
CGGAAAG750.002662202610.12703114
ATCCGAA750.002662202610.12703112
GTCTAGA2450.010.1017981
GTGTAAG3350.09.9452271
TGGACTG3550.09.91694455
TTAGGAC1451.5815021E-79.8430073
TAAGACT2950.09.6761754
TACACGG1201.6698232E-59.5149075
ACACCGT800.00453718949.4948686
CTGCTCG1304.2741594E-69.4940919
GTATTAG3050.09.3629531
Produced by FastQC (version 0.11.5)