FastQCFastQC Report
Wed 25 May 2016
SRR1294705_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294705_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1915715
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC48580.25358678091469766No Hit
CCCATGTACTCTGCGTTGATACCAC41330.2157419031536528No Hit
GTATCAACGCAGAGTACTTTTTTTT30200.15764349081152468No Hit
GGTATCAACGCAGAGTACTTTTTTT24540.12809838624221245No Hit
GAGTACATGGGAAGCAGTGGTATCA23280.1215212074864998No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTCG456.775473E-414.7742865
TAGGACG400.00528798214.2466324
ACGTCGC551.9666372E-413.8131136
CTAAGAC1501.4551915E-1112.0308043
CGACTGC657.996925E-411.69717815
CCTATAC1451.2223609E-911.135593
GTATTAG2650.011.1131921
TTGCGGA600.00577115511.110319
TTGGCGT600.005886849511.0807144
GCTACGT700.00149429510.854863
CTAGGAC2300.010.7368733
TGCTACG750.002652673510.1312042
CTACGTC750.002653248110.1309394
GTCCTAT1651.02209015E-89.7878651
CTTCGAG800.0044266189.52311319
GTCCTAC1304.2387383E-69.4999871
TATTAGG1701.670196E-89.4980032
CTAGACA2201.6370905E-119.4977554
CAGGACA4550.09.3933854
TAGACAG3250.09.3516365
Produced by FastQC (version 0.11.5)