##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294705_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1915715
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.943148119631573	33.0	33.0	33.0	27.0	33.0
2	30.935873029130114	33.0	33.0	33.0	27.0	33.0
3	30.830878810261442	33.0	33.0	33.0	27.0	33.0
4	34.28253419741454	37.0	37.0	37.0	27.0	37.0
5	34.48726715612709	37.0	37.0	37.0	27.0	37.0
6	34.73676773424022	37.0	37.0	37.0	27.0	37.0
7	34.67236828025045	37.0	37.0	37.0	27.0	37.0
8	34.60885309140451	37.0	37.0	37.0	27.0	37.0
9	34.3376279874616	37.0	37.0	37.0	27.0	37.0
10	34.42062624137724	37.0	37.0	37.0	27.0	37.0
11	34.656768882636506	37.0	37.0	37.0	27.0	37.0
12	34.58413020725943	37.0	37.0	37.0	27.0	37.0
13	34.51093038369486	37.0	37.0	37.0	27.0	37.0
14	35.64013958234915	40.0	37.0	40.0	27.0	40.0
15	35.961364816791644	40.0	37.0	40.0	27.0	40.0
16	35.972063172235956	40.0	37.0	40.0	27.0	40.0
17	35.907390713127995	40.0	37.0	40.0	27.0	40.0
18	35.91081136807928	40.0	37.0	40.0	27.0	40.0
19	35.9993375841396	40.0	37.0	40.0	27.0	40.0
20	35.92172374283231	40.0	37.0	40.0	27.0	40.0
21	35.861100946643944	40.0	37.0	40.0	27.0	40.0
22	35.72075230397006	37.0	37.0	40.0	27.0	40.0
23	35.3337464079991	37.0	33.0	40.0	27.0	40.0
24	35.65332578175772	37.0	37.0	40.0	27.0	40.0
25	34.48902263645689	37.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.33117037849536857
1101	2	0.4999184468676141
1101	3	0.2617001362601066
1101	4	0.3409477317326406
1101	5	0.36243454485033055
1101	6	0.29836290948124145
1101	7	0.2341081799244833
1101	8	0.4912335700069619
1101	9	0.6222892708043446
1101	10	0.5476940620536368
1101	11	0.416824258081931
1101	12	0.41987221742997605
1101	13	0.39227928089898256
1101	14	0.9574000260609665
1101	15	0.8465862600751564
1101	16	0.7092652550065139
1101	17	0.6037595361219914
1101	18	0.77942831600663
1101	19	0.6608496311457728
1101	20	0.739610783972978
1101	21	0.7333017428601067
1101	22	0.8474182716813274
1101	23	1.2652598697445399
1101	24	0.9623387446363196
1101	25	0.8090408701389435
1102	1	0.1980203592082148
1102	2	0.3426837989255702
1102	3	0.13597094704871893
1102	4	0.09462747125149917
1102	5	0.08443333899379013
1102	6	0.029140445074290255
1102	7	0.19980299743726704
1102	8	0.09161734783507569
1102	9	0.37344102001440405
1102	10	0.4162417154331237
1102	11	0.3168461277743404
1102	12	0.2647480703920735
1102	13	0.18862041585035882
1102	14	0.8013896273749666
1102	15	0.6537219023071472
1102	16	0.433425822892886
1102	17	0.675841462811654
1102	18	0.6746227282498296
1102	19	0.5672707794406833
1102	20	0.6645920700167167
1102	21	0.621748674716109
1102	22	0.7664687894110216
1102	23	1.0032484828911024
1102	24	0.7665945414275726
1102	25	0.7623208578838572
1103	1	0.2857611342845736
1103	2	0.49662743193006165
1103	3	0.26088302797818486
1103	4	0.047525245245772396
1103	5	0.1960970595758198
1103	6	0.14742710161888084
1103	7	0.18730327894004262
1103	8	0.36972703276224195
1103	9	0.5961378949665814
1103	10	0.6359618152039275
1103	11	0.4192016558853666
1103	12	0.4301172944061946
1103	13	0.4307154136114022
1103	14	0.9591373115558497
1103	15	0.80992614255387
1103	16	0.5141610670774099
1103	17	0.5446700044182649
1103	18	0.48697154881853066
1103	19	0.5663325198108922
1103	20	0.8768578519331243
1103	21	0.8279132184060245
1103	22	0.7866248856537368
1103	23	1.2808868606338564
1103	24	0.88619023252528
1103	25	0.8336585752366545
1104	1	0.2501770808320636
1104	2	0.45297043758024813
1104	3	0.1683843288942164
1104	4	0.2738166967136024
1104	5	0.23151816006463122
1104	6	0.03643742574759301
1104	7	0.2061456384398781
1104	8	0.2145752575841655
1104	9	0.4587001161680888
1104	10	0.6139859342117759
1104	11	0.41733692752204377
1104	12	0.28669530186067504
1104	13	0.3772092418294406
1104	14	0.8405393076558099
1104	15	0.6524732106066722
1104	16	0.5863711375705662
1104	17	0.5037590875050739
1104	18	0.4467713972870442
1104	19	0.48527991258809067
1104	20	0.5766546776487758
1104	21	0.6757942883423809
1104	22	0.7192957733437026
1104	23	1.1761882276172173
1104	24	0.916154131797704
1104	25	0.8374522777692235
1105	1	0.237087611931166
1105	2	0.351488096845614
1105	3	0.2964136398581445
1105	4	0.17771450129491484
1105	5	0.1852791562734808
1105	6	0.15823704382623305
1105	7	0.1892657500933197
1105	8	0.17994028477646395
1105	9	-1.5570335725669722
1105	10	-0.10180277791185688
1105	11	0.11327269775753734
1105	12	0.2702047390735203
1105	13	0.15075775193931662
1105	14	-1.885066264176082
1105	15	-0.5077716410527913
1105	16	-0.057685610561684086
1105	17	0.086701401507149
1105	18	0.024441483437115608
1105	19	0.18894097625745587
1105	20	0.33446514361025237
1105	21	0.3520673030723884
1105	22	0.3422321543049307
1105	23	0.7650693896802139
1105	24	0.46978185235825265
1105	25	0.1681597807913917
1106	1	0.189020516117683
1106	2	0.21214110919795104
1106	3	0.22183747022679157
1106	4	-0.882977506545437
1106	5	-0.04739817865502971
1106	6	0.14468954963012948
1106	7	0.18878665565302555
1106	8	0.34247829187128076
1106	9	0.6183014865868302
1106	10	0.5190493471703448
1106	11	0.4742864429303708
1106	12	0.2748684815697189
1106	13	0.21015318435949837
1106	14	0.7710367507055551
1106	15	0.5174686060234066
1106	16	0.28223619218891827
1106	17	0.37137435754685555
1106	18	0.4048267529651852
1106	19	0.40005218559397804
1106	20	0.4832260546116487
1106	21	0.6545428780962226
1106	22	0.817346746524592
1106	23	1.1073748775830197
1106	24	0.6859116434868326
1106	25	0.5132141068103735
1107	1	0.18756931433986068
1107	2	0.28703467193064114
1107	3	0.24047724548231386
1107	4	0.30486024159627334
1107	5	0.16323985877882308
1107	6	0.15192573218070748
1107	7	0.15818944323621764
1107	8	0.13981992468232107
1107	9	0.2407463672107184
1107	10	0.29410143440968284
1107	11	-0.015068106309200857
1107	12	0.13174343685270173
1107	13	0.17154957924154957
1107	14	0.5880620585956606
1107	15	0.5377000914557897
1107	16	0.3108499087395131
1107	17	0.39308980131634286
1107	18	0.3639948398011015
1107	19	0.22336847689633288
1107	20	0.43748195057681727
1107	21	0.36758636760076513
1107	22	0.3757085909947264
1107	23	0.6077472679412139
1107	24	0.5849723060485843
1107	25	0.4561198549831147
1108	1	0.49720124036346647
1108	2	0.46169935708673293
1108	3	0.567260539692775
1108	4	0.7697976649368243
1108	5	0.5880038463227493
1108	6	0.5184473053065162
1108	7	0.5411302485638032
1108	8	0.5045339124943524
1108	9	0.2301605745535582
1108	10	0.48141467958146933
1108	11	0.38145055614641166
1108	12	0.49053901609875794
1108	13	0.423365173366804
1108	14	-0.8877659734544281
1108	15	0.1510900818025931
1108	16	0.5065834739365371
1108	17	0.5951281228949412
1108	18	0.7040361886416946
1108	19	0.5547697961710085
1108	20	0.697738075805205
1108	21	0.8110949612273401
1108	22	0.7647876519192991
1108	23	1.033408929822528
1108	24	0.8618796096584305
1108	25	0.7694685569850748
1109	1	0.3566556336354658
1109	2	0.29106985356417425
1109	3	0.5028930816545092
1109	4	0.6246583559345424
1109	5	0.5270980406618406
1109	6	0.34245838786158345
1109	7	0.4009518882674712
1109	8	0.4498832160713775
1109	9	0.5394286143850948
1109	10	0.5431116260096971
1109	11	0.32790137197272173
1109	12	0.3098630805938143
1109	13	0.27671555631912526
1109	14	0.6822745053895076
1109	15	0.43507578745894904
1109	16	0.4817370675194965
1109	17	0.46756760073976267
1109	18	0.5740449151434603
1109	19	0.4940291662622087
1109	20	0.6603221919826723
1109	21	0.6732600531995843
1109	22	0.6549263643589853
1109	23	1.0489884656934194
1109	24	0.7926647257679065
1109	25	0.6865109369962425
1110	1	0.3819637167652168
1110	2	0.23076839814447325
1110	3	0.41083527080397886
1110	4	0.5687178288983219
1110	5	0.38516344968065397
1110	6	0.20537709174335106
1110	7	0.15901604824787796
1110	8	0.24601516716528238
1110	9	0.30506596188251933
1110	10	0.3176114586162413
1110	11	0.3075752792704307
1110	12	0.19738438394755065
1110	13	0.15913632211795203
1110	14	0.5993800640788081
1110	15	0.31481658522424283
1110	16	0.3499945184239266
1110	17	0.4941945346025136
1110	18	0.4195552577390771
1110	19	0.44586501852683824
1110	20	0.5593879558622206
1110	21	0.48927680726548317
1110	22	0.7369216095374185
1110	23	0.8560537406636541
1110	24	0.7776835137590226
1110	25	0.4917072267276481
1111	1	0.1633539236188959
1111	2	0.0797354982244336
1111	3	0.2822361191236631
1111	4	0.5428462941356571
1111	5	0.37062413132096594
1111	6	0.3812480074372644
1111	7	0.2815075979594255
1111	8	0.297302080768695
1111	9	0.3780561225992827
1111	10	0.2896051141390217
1111	11	0.2350679921795802
1111	12	0.41125732367045487
1111	13	0.3657948301101328
1111	14	-1.562078931087889
1111	15	-0.4402231231487619
1111	16	0.020318903933024046
1111	17	0.2667307550118423
1111	18	0.2800070606301972
1111	19	0.23579250928479212
1111	20	0.3391868301997363
1111	21	0.5791027055831179
1111	22	0.3290624991587663
1111	23	-1.7481547538719298
1111	24	-0.3488231855423436
1111	25	0.09952749868781297
1112	1	-0.23975725410442905
1112	2	-0.2769431280105934
1112	3	0.02561143399576693
1112	4	0.18973536462381446
1112	5	-0.7589186008198752
1112	6	-0.9706127812013676
1112	7	-0.9483228165653657
1112	8	-0.7554162415583079
1112	9	-0.7091101504673532
1112	10	-0.9005351232335386
1112	11	-0.9055087435606453
1112	12	-0.828321887107883
1112	13	-0.8392995678835575
1112	14	-0.5934982793098982
1112	15	-0.8792576840093176
1112	16	-0.921607215022803
1112	17	-1.060763165655338
1112	18	-1.0747778704615456
1112	19	-0.9311799856091767
1112	20	-1.0044985060893197
1112	21	-1.70470673433244
1112	22	-1.6843532784091053
1112	23	-1.4080730257539074
1112	24	-1.6618191265981324
1112	25	-1.9956326626728895
1113	1	0.17139907631778328
1113	2	-0.04753205140919903
1113	3	-0.01897414873555192
1113	4	0.32250219634282473
1113	5	0.08657218890767382
1113	6	-0.04557292714943628
1113	7	-0.07372674559522352
1113	8	0.06351191149465052
1113	9	0.2564000337129855
1113	10	-0.10575827453496345
1113	11	-0.014749024257049825
1113	12	-0.0378237250608251
1113	13	0.020439803985610183
1113	14	0.15259489358323464
1113	15	0.01074921328420686
1113	16	0.06219753799926053
1113	17	0.10519375220869875
1113	18	0.19855762385635245
1113	19	0.0497103679315245
1113	20	-0.5691550874173217
1113	21	-0.4482997429544753
1113	22	0.06141815331919531
1113	23	-0.22501224293620226
1113	24	0.05195020246010529
1113	25	-0.9870676512869139
1114	1	-0.7395721451909729
1114	2	-0.6417304273514794
1114	3	-0.47507799153133945
1114	4	-1.437938775534768
1114	5	-0.7198623028887638
1114	6	-0.41448418267356857
1114	7	-0.5096901025195066
1114	8	-0.3792419043008053
1114	9	-0.3877064906117127
1114	10	-0.46807421381868863
1114	11	-0.44628760614209995
1114	12	-0.6089801301971107
1114	13	-0.48745043501364194
1114	14	-0.6080630903578736
1114	15	-1.1593356737601752
1114	16	-0.6371028083789056
1114	17	-0.7587741703802422
1114	18	-0.6990627295200369
1114	19	-0.6212627545356213
1114	20	-1.8527520034908491
1114	21	-6.834146350753112
1114	22	-7.70772096487163
1114	23	-7.823014217914206
1114	24	-5.597326760447654
1114	25	-7.033098601581056
1115	1	-0.9569872370485477
1115	2	-1.010862767385163
1115	3	-0.6734747161872114
1115	4	-0.494557610273084
1115	5	-0.43738349622852013
1115	6	-0.7470523776760203
1115	7	-0.8382332342815886
1115	8	-0.4494674993424965
1115	9	-2.3376903166052827
1115	10	-1.3649468079352332
1115	11	-0.9951962920148603
1115	12	-0.8040247381068468
1115	13	-1.1928234399499473
1115	14	-0.822661425082174
1115	15	-1.700982662052354
1115	16	-1.0931649338158778
1115	17	-2.855638138360021
1115	18	-2.5532702355529437
1115	19	-1.8046081644604186
1115	20	-1.4712383537076903
1115	21	-1.096611118375769
1115	22	-0.7153052799671542
1115	23	-0.10618893815780694
1115	24	-1.1185627995844385
1115	25	-0.8147733444682643
1116	1	-1.3503888105561188
1116	2	-0.699643623764711
1116	3	-0.4678014939606605
1116	4	-0.12452905418493998
1116	5	-0.10257169795271182
1116	6	-0.2992770030262051
1116	7	-0.47403377899059507
1116	8	-1.405350291647295
1116	9	-0.745876539537619
1116	10	-2.4035215582047726
1116	11	-1.1288065263090061
1116	12	-0.6634878663299659
1116	13	-0.721963314987299
1116	14	-1.0033685292480286
1116	15	-1.0246022707105382
1116	16	-0.6360184425992017
1116	17	-0.4409026339845923
1116	18	-1.023583486530434
1116	19	-0.5537812502932269
1116	20	-0.8214730375556414
1116	21	-0.2820178531960096
1116	22	0.036605531510709
1116	23	0.19876561890890088
1116	24	-0.07576125032464631
1116	25	0.037317268610813414
1201	1	0.1850775730275025
1201	2	0.37141293133142383
1201	3	0.1900827008331234
1201	4	0.27160899082453227
1201	5	0.2242855320845436
1201	6	0.2431687815151946
1201	7	0.30412820322413836
1201	8	0.42501662308246324
1201	9	0.6672736668365005
1201	10	0.6152613186815117
1201	11	0.5688197602175649
1201	12	0.5158580035383196
1201	13	0.6095478387162032
1201	14	1.0106370928251422
1201	15	0.7075131142562157
1201	16	0.7514644965921491
1201	17	0.5853746655931573
1201	18	0.4291653820204928
1201	19	0.5647182847026713
1201	20	0.7050637142125638
1201	21	0.7033313201198794
1201	22	0.8287372784096121
1201	23	0.670800966529157
1201	24	0.6537440821487195
1201	25	0.6052971683745625
1202	1	-0.3308672211477628
1202	2	0.03887446689398644
1202	3	-0.20647042052367937
1202	4	-0.3796982569166687
1202	5	-0.3120268230649472
1202	6	-0.34259700582005337
1202	7	-0.29142836387823934
1202	8	-0.08820832530771128
1202	9	-0.0422225656031614
1202	10	0.27387387219106785
1202	11	-0.09004342208082505
1202	12	-0.15605130012253454
1202	13	-0.1605014175382493
1202	14	0.23965108838008575
1202	15	0.2131986314099379
1202	16	0.2772403435635056
1202	17	0.06957914025920076
1202	18	-0.08686414562244948
1202	19	0.026495731757187002
1202	20	0.21568360110816087
1202	21	0.17470039866066145
1202	22	0.09554227738862409
1202	23	0.470144109731919
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2211	3	0.2509101924959367
2211	4	0.40753368426851466
2211	5	0.29072367239133
2211	6	0.16613292366268695
2211	7	0.15859025026025364
2211	8	0.3070121141212496
2211	9	0.39693795140763655
2211	10	0.1590262161181073
2211	11	0.046477359798679174
2211	12	0.157530075907637
2211	13	0.05747758535274983
2211	14	0.38745073034823463
2211	15	0.2520495291165119
2211	16	0.24163852061812463
2211	17	0.3832799128530553
2211	18	0.26498952267067466
2211	19	0.339256326877468
2211	20	0.24871653764804336
2211	21	0.2748329637971807
2211	22	0.36263047367972945
2211	23	0.5572511749643851
2211	24	0.5217868083443165
2211	25	0.6552236702446308
2212	1	-0.11738804220399501
2212	2	-0.1523512823301374
2212	3	0.06969603505827493
2212	4	-0.19415895265333916
2212	5	0.0069965221448384796
2212	6	-0.4017177285509703
2212	7	-0.3731877866311848
2212	8	-0.10233164725657673
2212	9	-0.07995228528194076
2212	10	-0.4851198379547128
2212	11	-0.3650486404133204
2212	12	-0.48227684857199193
2212	13	-0.45082122082972376
2212	14	-0.24860467900781202
2212	15	-0.37498503797677074
2212	16	-0.2604146734697821
2212	17	-0.250510300331932
2212	18	-0.20337531140697251
2212	19	-0.3967611371781956
2212	20	-0.38719062387450265
2212	21	-0.30744458379959383
2212	22	-0.26645191063703066
2212	23	0.3085704270579086
2212	24	-0.08890362713561473
2212	25	-0.8332752167444326
2213	1	-0.23759053965085997
2213	2	-1.4004535182123838
2213	3	-0.3293768799340384
2213	4	-0.032661718484000346
2213	5	2.5037721002263424E-5
2213	6	-0.0563887919710524
2213	7	0.001938733682692373
2213	8	0.18875843260912006
2213	9	0.07176006183427575
2213	10	-0.323504729229235
2213	11	-0.09794164895841817
2213	12	-0.021906891321819444
2213	13	-0.44174942613278034
2213	14	-0.024434549979012843
2213	15	-0.15771008339743986
2213	16	-0.16255573651643118
2213	17	-0.0022258946811035685
2213	18	0.07857056046695732
2213	19	0.07721180362961633
2213	20	0.2290107954910212
2213	21	-0.3201977664937914
2213	22	-0.8576645735466499
2213	23	-0.1607518267165986
2213	24	0.0399605394864011
2213	25	-0.55969486640047
2214	1	-0.19867912127005027
2214	2	-0.421180749483419
2214	3	-0.4843693215732756
2214	4	-0.5535777237015651
2214	5	-0.3523808307057763
2214	6	-0.2647223844058786
2214	7	-0.5388833731529985
2214	8	-0.22688034141144442
2214	9	-0.16511644743193443
2214	10	-1.820715760734494
2214	11	-0.8323454416120768
2214	12	-0.5205321728170844
2214	13	-0.3777078277578667
2214	14	-0.6818425578438578
2214	15	-0.44372319245252356
2214	16	-0.6314876905414621
2214	17	-0.4324386096057893
2214	18	-0.48437851794604114
2214	19	-1.472127487557529
2214	20	-2.40593444525868
2214	21	-1.8077539359965087
2214	22	-1.834375346690706
2214	23	-2.474104437201177
2214	24	-1.451502288332513
2214	25	-1.9452947455851444
2215	1	-0.0510966981870169
2215	2	-0.2757663474371945
2215	3	-0.6433333604789482
2215	4	-0.2033906878659053
2215	5	-0.09481031610843615
2215	6	0.014807207656765797
2215	7	-0.17605541743380826
2215	8	-0.2049311563582421
2215	9	-0.18854276746056797
2215	10	-0.10409716129824176
2215	11	-0.41109883545135517
2215	12	-0.4281441662983809
2215	13	-0.2556873975526912
2215	14	0.10271643545680575
2215	15	-0.5156133296978709
2215	16	-0.33898281506616
2215	17	-0.1012004891561773
2215	18	-0.10603995251594256
2215	19	-2.0704550961519743
2215	20	-2.1778727981206245
2215	21	-3.1783299256482564
2215	22	-2.126407463791537
2215	23	-3.308451742775212
2215	24	-2.285217073076595
2215	25	-1.32484038314022
2216	1	-1.4150842255666767
2216	2	-0.9910789898820198
2216	3	-0.7040294795255093
2216	4	-0.7175139538207347
2216	5	-1.5125432600421505
2216	6	-1.3527980653734133
2216	7	-0.8711567272202956
2216	8	-5.102178466798215
2216	9	-3.8560792635901002
2216	10	-1.9062514313151482
2216	11	-0.9468388224624107
2216	12	-1.0838010159532843
2216	13	-1.7299388184713536
2216	14	-3.209394954885383
2216	15	-1.6709248830070251
2216	16	-2.905591125574702
2216	17	-2.5423552956732394
2216	18	-2.4516842166031054
2216	19	-1.524894735849756
2216	20	-0.8990810933005946
2216	21	-0.6309135974876412
2216	22	-0.6705513426473786
2216	23	-0.8682089865358762
2216	24	-0.5246332963574716
2216	25	-0.2358658648810632
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	4.0
7	57.0
8	266.0
9	811.0
10	1490.0
11	2619.0
12	4212.0
13	5799.0
14	7654.0
15	9443.0
16	10608.0
17	11283.0
18	11760.0
19	12047.0
20	12473.0
21	13180.0
22	13856.0
23	14506.0
24	15456.0
25	16469.0
26	17636.0
27	19601.0
28	22322.0
29	26206.0
30	31760.0
31	38531.0
32	51423.0
33	72073.0
34	105125.0
35	164683.0
36	285883.0
37	916479.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.955764467039	20.489141105063307	12.800191320403192	23.754903107494496
2	15.753525137865271	20.702631278070978	39.64980680850575	23.894036775558
3	19.346565886660606	25.033427712343638	28.768403515431046	26.851602885564713
4	12.52920547024005	16.598209479514793	37.251691502519954	33.620893547725196
5	13.367648847764105	37.9083755925117	34.75685809948297	13.96711746024122
6	32.215770988030705	37.62628108216467	16.80973998312772	13.348207946676913
7	27.10427314378123	32.478106017145606	21.965235847406888	18.45238499166627
8	24.768570477036615	37.40618321638752	20.055102515944547	17.77014379063132
9	24.333366219364443	17.28401055067398	21.436420078853395	36.94620315110818
10	14.729286313092665	30.707506444072553	33.13145908000881	21.431748162825976
11	33.21516938977919	25.391971603069376	22.18865166779767	19.20420733935376
12	20.965947737427005	27.39376709908215	30.84388619560854	20.796398967882308
13	27.133630218613153	23.485292589996067	26.02555663379909	23.355520557591692
14	20.623123405285995	23.175187816071794	27.53353336423581	28.6681554144064
15	23.283236318326153	30.691716740191353	23.433261139760596	22.59178580172189
16	22.089933072989123	30.89453345019416	25.40397114532834	21.611562331488372
17	20.75093191792127	29.982413491237804	27.770971350538424	21.495683240302494
18	20.972041766913975	29.512564474528354	28.712169418780498	20.803224339777177
19	21.428806328303175	28.749586967971684	28.892249405051178	20.929357298673963
20	22.380661302926395	28.323701534110455	28.426744646743256	20.868892516219894
21	22.425734013803396	28.439681595179522	27.87938565443872	21.255198736578365
22	21.620994738359823	28.209537819867187	28.58143631492943	21.58803112684356
23	21.74094294981734	28.473112123065864	28.400602436388162	21.385342490728636
24	21.732825527276827	28.24561568352324	28.340516116369436	21.681042672830497
25	22.14700810701087	28.0155401813928	27.695989185071717	22.141462526524617
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1958.0
1	1958.0
2	2151.5
3	2345.0
4	2345.0
5	2345.0
6	3234.5
7	4124.0
8	4124.0
9	4124.0
10	6152.0
11	8180.0
12	8180.0
13	8180.0
14	13079.5
15	17979.0
16	17979.0
17	17979.0
18	27554.0
19	37129.0
20	37129.0
21	37129.0
22	52076.5
23	67024.0
24	67024.0
25	67024.0
26	86414.0
27	105804.0
28	105804.0
29	105804.0
30	126025.5
31	146247.0
32	146247.0
33	146247.0
34	163894.5
35	181542.0
36	181542.0
37	181542.0
38	194081.0
39	206620.0
40	206620.0
41	206620.0
42	209663.5
43	212707.0
44	212707.0
45	212707.0
46	218458.0
47	224209.0
48	224209.0
49	224209.0
50	215162.5
51	206116.0
52	206116.0
53	206116.0
54	189678.5
55	173241.0
56	173241.0
57	173241.0
58	153105.0
59	132969.0
60	132969.0
61	132969.0
62	112084.0
63	91199.0
64	91199.0
65	91199.0
66	71714.0
67	52229.0
68	52229.0
69	52229.0
70	38971.0
71	25713.0
72	25713.0
73	25713.0
74	18572.5
75	11432.0
76	11432.0
77	11432.0
78	7998.0
79	4564.0
80	4564.0
81	4564.0
82	3088.0
83	1612.0
84	1612.0
85	1612.0
86	1072.0
87	532.0
88	532.0
89	532.0
90	362.0
91	192.0
92	192.0
93	192.0
94	112.5
95	33.0
96	33.0
97	33.0
98	24.0
99	15.0
100	15.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.031476498330910395
2	0.013467556499792505
3	0.020253534581083303
4	0.021193131546185105
5	0.018374340650879697
6	0.007673375214998056
7	3.131989883672676E-4
8	6.785978081290798E-4
9	4.175986511563568E-4
10	5.21998313945446E-5
11	7.82997470918169E-4
12	0.002192392918570873
13	0.0025055919069381406
14	5.741981453399905E-4
15	0.0016703946046254272
16	0.002401192244149051
17	0.002192392918570873
18	0.0018791939302036055
19	2.087993255781784E-4
20	6.263979767345352E-4
21	0.08080533899875504
22	0.07741234995810964
23	0.07767334911508236
24	0.0012005961220745256
25	0.22357187786283453
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1915715.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.95253882132194
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.82407219446482	75.3408737462189
2	6.888220149436508	11.42790698711888
3	1.1739244467781411	2.9214003973398786
4	0.3941885005106767	1.3079574756612238
5	0.20575358374399755	0.8533891071575036
6	0.11503026169692655	0.5725231349424674
7	0.08570023480097458	0.4976336438026975
8	0.05299926663207028	0.3517138978238722
9	0.042530757057880085	0.3175230848349609
>10	0.19934823073968094	2.8547533236815354
>50	0.011096334814137358	0.6204947367109453
>100	0.005431949305174598	0.9194705078719839
>500	6.31144451508256E-4	0.33381570070906447
>1k	0.0010729455675639618	1.6805442561260473
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	4858	0.25358678091469766	No Hit
CCCATGTACTCTGCGTTGATACCAC	4133	0.2157419031536528	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3020	0.15764349081152468	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2454	0.12809838624221245	No Hit
GAGTACATGGGAAGCAGTGGTATCA	2328	0.1215212074864998	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	5.21998313945446E-5	0.0
12	0.0	0.0	0.0	5.21998313945446E-5	0.0
13	0.0	0.0	0.0	1.043996627890892E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGTCG	45	6.775473E-4	14.774286	5
TAGGACG	40	0.005287982	14.246632	4
ACGTCGC	55	1.9666372E-4	13.813113	6
CTAAGAC	150	1.4551915E-11	12.030804	3
CGACTGC	65	7.996925E-4	11.697178	15
CCTATAC	145	1.2223609E-9	11.13559	3
GTATTAG	265	0.0	11.113192	1
TTGCGGA	60	0.005771155	11.1103	19
TTGGCGT	60	0.0058868495	11.080714	4
GCTACGT	70	0.001494295	10.85486	3
CTAGGAC	230	0.0	10.736873	3
TGCTACG	75	0.0026526735	10.131204	2
CTACGTC	75	0.0026532481	10.130939	4
GTCCTAT	165	1.02209015E-8	9.787865	1
CTTCGAG	80	0.004426618	9.523113	19
GTCCTAC	130	4.2387383E-6	9.499987	1
TATTAGG	170	1.670196E-8	9.498003	2
CTAGACA	220	1.6370905E-11	9.497755	4
CAGGACA	455	0.0	9.393385	4
TAGACAG	325	0.0	9.351636	5
>>END_MODULE