FastQCFastQC Report
Wed 25 May 2016
SRR1294705_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294705_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1915715
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT82070.42840401625502744No Hit
GGTATCAACGCAGAGTACTTTTTTT68180.35589845044800505No Hit
TATCAACGCAGAGTACTTTTTTTTT53830.2809916923968335No Hit
GTACATGGGAAGCAGTGGTATCAAC51530.2689857311760883No Hit
CCCATGTACTCTGCGTTGATACCAC46450.24246821682765962No Hit
ACGCAGAGTACTTTTTTTTTTTTTT36470.19037278509590413No Hit
GAGTACATGGGAAGCAGTGGTATCA26930.1405741459455086No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGAA500.001507491713.2909387
TATACCG701.07401145E-412.2350235
TATACTG4650.011.6649325
CAGGACT2400.010.3091394
TTAGACA1502.381421E-810.15053754
ATTAGAC1502.5836562E-79.51612953
CTATACT3100.09.51612954
GTAGGAC1401.034361E-69.5161293
TCTAAGC1401.034361E-69.5161293
CTAGCAC2054.0927262E-109.2840283
GCCTTAG1651.030985E-79.2318681
GTACTAG1451.6572521E-69.1920751
GGATTAG1451.6572521E-69.1920751
GTCCTAG2304.0017767E-119.1064351
TGTACTG3250.09.0769235
TACACAG4750.09.0152815
GTGTAGC1804.0736268E-88.9914541
GTATAAC1804.0736268E-88.9914541
TAACACT2451.6370905E-118.9335094
GTAGCAC1606.576647E-78.92137053
Produced by FastQC (version 0.11.5)