FastQCFastQC Report
Wed 25 May 2016
SRR1294704_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294704_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4298121
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT75960.17672838898672236No Hit
GTACATGGGAAGCAGTGGTATCAAC62390.1451564532501528No Hit
ATTCCATTCCATTCCATTCCATTCC62180.14466786765658762No Hit
CCCATGTACTCTGCGTTGATACCAC57210.13310467527554481No Hit
GAATGGAATGGAATGGAATGGAATG55060.12810248943666314No Hit
GGTATCAACGCAGAGTACTTTTTTT51580.1200059281718686No Hit
TATCAACGCAGAGTACTTTTTTTTT44610.10378953966163353No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACCG602.5767336E-514.2462465
TAGTCCG957.424023E-812.9965765
TAAACCG751.4840347E-512.663335
CTACGAC600.00588801411.0809313
CGTGCGA600.005897211.0786110
ACGTCGC700.001497065910.8530216
GTCGTAC909.519345E-510.5559691
CTATCGC750.002658839310.1288979
TACGACC1606.21003E-910.0910914
AACCGCT951.6526428E-49.9956237
GTCTTAG2950.09.9834421
TACCGTG1252.5873815E-69.8756747
TATTCCG1856.530172E-109.7541875
GTCCTAC2700.09.5003731
GTACGAC1606.667506E-89.497943
TAGACTG5000.09.49749855
ACTATCG6150.09.41874911
TATCGGT6050.09.41670513
ATCGCGC2550.09.3096488
CTATCGG6350.09.27163812
Produced by FastQC (version 0.11.5)