##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294704_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4298121
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.248026288696853	33.0	33.0	33.0	27.0	33.0
2	31.232029996363526	33.0	33.0	33.0	27.0	33.0
3	31.207031630798667	33.0	33.0	33.0	27.0	33.0
4	34.6488284066456	37.0	37.0	37.0	27.0	37.0
5	34.96994198162406	37.0	37.0	37.0	33.0	37.0
6	35.183580452946764	37.0	37.0	37.0	33.0	37.0
7	35.11257244735548	37.0	37.0	37.0	33.0	37.0
8	35.03414561851562	37.0	37.0	37.0	33.0	37.0
9	34.73977745158873	37.0	37.0	37.0	27.0	37.0
10	34.81715847459855	37.0	37.0	37.0	33.0	37.0
11	35.06193287718052	37.0	37.0	37.0	33.0	37.0
12	34.99046443783225	37.0	37.0	37.0	33.0	37.0
13	34.929046204143624	37.0	37.0	37.0	33.0	37.0
14	36.15623152535724	40.0	37.0	40.0	27.0	40.0
15	36.47298389226362	40.0	37.0	40.0	33.0	40.0
16	36.504053282818234	40.0	37.0	40.0	33.0	40.0
17	36.44472479951123	40.0	37.0	40.0	33.0	40.0
18	36.44856554759626	40.0	37.0	40.0	33.0	40.0
19	36.530385952373145	40.0	37.0	40.0	33.0	40.0
20	36.42390523673019	40.0	37.0	40.0	27.0	40.0
21	36.37591822100867	40.0	37.0	40.0	27.0	40.0
22	36.236844425738596	40.0	37.0	40.0	27.0	40.0
23	35.84610344846038	40.0	37.0	40.0	27.0	40.0
24	36.16499814686464	40.0	37.0	40.0	27.0	40.0
25	35.13439291262391	37.0	33.0	40.0	27.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.45802729605075854
1101	2	0.6147326962619282
1101	3	0.43918264399676943
1101	4	0.48836552328897653
1101	5	0.5309816964620282
1101	6	0.3895229906850659
1101	7	0.4169688205363542
1101	8	0.5562979505582462
1101	9	0.7823258115895584
1101	10	0.7483553445217126
1101	11	0.5263280350553785
1101	12	0.4974691036952592
1101	13	0.5170984882178686
1101	14	1.2486762079198144
1101	15	0.9355767789012859
1101	16	0.9165481726489446
1101	17	0.9593982236963825
1101	18	0.9500051518146222
1101	19	0.9058156036463956
1101	20	1.0610954329881892
1101	21	1.1070136495246317
1101	22	1.070114303300329
1101	23	1.415713760621017
1101	24	1.1977492610931932
1101	25	1.0205918360221062
1102	1	0.3565325536964963
1102	2	0.48914915655017666
1102	3	0.36299360136542447
1102	4	0.30418446963923884
1102	5	0.3989153859671859
1102	6	0.31637382371815903
1102	7	0.3305899043503331
1102	8	0.4035777585803402
1102	9	0.5930270870785108
1102	10	0.700521154839663
1102	11	0.44934577440294987
1102	12	0.5372853971496809
1102	13	0.5115895098099728
1102	14	1.0995420505875089
1102	15	0.8475665535674253
1102	16	0.6286351047566683
1102	17	0.8615617078617746
1102	18	0.7251235151120454
1102	19	0.7974754270968205
1102	20	0.9412529730223582
1102	21	0.9549239530821296
1102	22	0.9849079032432044
1102	23	1.3731076799500457
1102	24	1.097220575304469
1102	25	0.9578456560514752
1103	1	0.30919142383558196
1103	2	0.5002102152254011
1103	3	0.26997737272377975
1103	4	0.19000215165532097
1103	5	0.28651641773591763
1103	6	0.3128272511182928
1103	7	0.3050954710286504
1103	8	0.5116565924909935
1103	9	0.7179749160209994
1103	10	0.5968215118274074
1103	11	0.5457401452252952
1103	12	0.46611109814860896
1103	13	0.4790782016670505
1103	14	1.033555196634694
1103	15	0.8496499531076864
1103	16	0.5272725662242337
1103	17	0.5655960930907398
1103	18	0.7315336696654953
1103	19	0.7424251627987744
1103	20	0.9284707991047085
1103	21	0.9240524580767797
1103	22	0.7628029996934558
1103	23	1.2756738603930486
1103	24	0.9290883521288649
1103	25	0.8526603296248467
1104	1	0.2900330420144286
1104	2	0.474345610610726
1104	3	0.34253683628391585
1104	4	0.33089358240312094
1104	5	0.3305621442873772
1104	6	0.2980881952905463
1104	7	0.2218565259414973
1104	8	0.4906335770763164
1104	9	0.6375598043799897
1104	10	0.6535637511885497
1104	11	0.49748265367573197
1104	12	0.3864139374546909
1104	13	0.47392141575242164
1104	14	0.9356531616986175
1104	15	0.8076146341023218
1104	16	0.695491464158664
1104	17	0.7914354964576731
1104	18	0.6606129599321235
1104	19	0.7350965675070071
1104	20	0.8693988673954323
1104	21	0.8822716354500955
1104	22	0.8963965216790868
1104	23	1.2751191742029206
1104	24	1.0553491907353134
1104	25	0.9428167326046974
1105	1	0.24154233915493606
1105	2	0.2882269232500754
1105	3	0.2972768626925557
1105	4	0.27037017179203815
1105	5	0.25951045261908234
1105	6	0.18479079502645845
1105	7	0.1723295003770673
1105	8	0.2683464155723243
1105	9	-1.4750963204766023
1105	10	-0.07870782488782169
1105	11	0.10717321799808843
1105	12	0.21119422621802642
1105	13	0.2749420447975979
1105	14	-1.6164497887094171
1105	15	-0.18907075672392182
1105	16	-0.053469507332991384
1105	17	0.09363585578836364
1105	18	0.18986540056679502
1105	19	0.282872938267019
1105	20	0.4010513556522497
1105	21	0.5349357694847754
1105	22	0.5395984611989633
1105	23	0.8968992681484167
1105	24	0.714531605177811
1105	25	0.3763567893183364
1106	1	0.005147106686944625
1106	2	0.0909098978015912
1106	3	0.14521544058152713
1106	4	-0.9929645673591523
1106	5	-0.1555138728020964
1106	6	0.046430280890831455
1106	7	0.10483657271464608
1106	8	0.25678714285141524
1106	9	0.4404900338240978
1106	10	0.4339757044348147
1106	11	0.2990896261195459
1106	12	0.11881782534638319
1106	13	0.231965228464027
1106	14	0.6113033922269011
1106	15	0.43445216516609264
1106	16	0.3293606943273062
1106	17	0.20407383466370987
1106	18	0.3161049113660397
1106	19	0.25037354125748834
1106	20	0.3462323631523958
1106	21	0.47082967974825607
1106	22	0.5709353740985108
1106	23	0.7440347203892799
1106	24	0.5497612515897643
1106	25	0.46115027427718047
1107	1	0.360495040193598
1107	2	0.2755417826044919
1107	3	0.3314762030872487
1107	4	0.4320274432226654
1107	5	0.46538428293053613
1107	6	0.30902170089908054
1107	7	0.2700674649906176
1107	8	0.32447915197019483
1107	9	0.506862782736853
1107	10	0.49642445773010024
1107	11	0.2666815846313284
1107	12	0.3299695548416963
1107	13	0.25969037508652093
1107	14	0.7798842876492813
1107	15	0.5723968808012714
1107	16	0.6600897623013751
1107	17	0.5709722723101436
1107	18	0.5730833501311992
1107	19	0.5064468064109278
1107	20	0.6146394450290771
1107	21	0.6579431462908545
1107	22	0.5383796480902774
1107	23	0.9059494532759587
1107	24	0.8134475112652737
1107	25	0.7115026224551713
1108	1	0.39314073462912447
1108	2	0.36554413361151106
1108	3	0.40962271775557113
1108	4	0.5989602285004736
1108	5	0.5135401581517058
1108	6	0.32021113989463146
1108	7	0.3315239478395213
1108	8	0.4513231708478074
1108	9	0.2149124958894717
1108	10	0.40224842828109075
1108	11	0.25717555695699446
1108	12	0.3217326696484193
1108	13	0.3841952868964498
1108	14	-1.162069861805655
1108	15	-0.03457271239536652
1108	16	0.2378415998547041
1108	17	0.35699498934744156
1108	18	0.5092255293151666
1108	19	0.5205768992561985
1108	20	0.5708077115844503
1108	21	0.6065473348066135
1108	22	0.785954872511681
1108	23	1.0098464620013274
1108	24	0.869031158512108
1108	25	0.7840220480826403
1109	1	0.4298738019971218
1109	2	0.31359701194538303
1109	3	0.43210470954868185
1109	4	0.5862889091453667
1109	5	0.5002967722283245
1109	6	0.3017878370164766
1109	7	0.3204525977229409
1109	8	0.3854812252579336
1109	9	0.4891671985907351
1109	10	0.47637284338228625
1109	11	0.30369439504178786
1109	12	0.37231627625757824
1109	13	0.3090684316957848
1109	14	0.7501762483211536
1109	15	0.46659026528694625
1109	16	0.49842528004732856
1109	17	0.522988539308102
1109	18	0.4670101124666317
1109	19	0.48489697278941435
1109	20	0.5908283364911284
1109	21	0.6024129760667662
1109	22	0.7404657766837559
1109	23	1.0709599521117141
1109	24	0.7680527496953076
1109	25	0.5986244263720053
1110	1	0.3619563399628909
1110	2	0.2277287934207486
1110	3	0.3722416809235227
1110	4	0.6163362478085048
1110	5	0.49157079972753337
1110	6	0.26534473421530436
1110	7	0.212129790818679
1110	8	0.28367606037975435
1110	9	0.3957966292757149
1110	10	0.43310135029744146
1110	11	0.21660981905173315
1110	12	0.2196654321926701
1110	13	0.18973060798286667
1110	14	0.6175093422034479
1110	15	0.34265659591501674
1110	16	0.32959938382110465
1110	17	0.395080520792348
1110	18	0.44878815749687817
1110	19	0.3921325643612761
1110	20	0.5147289131914619
1110	21	0.6017583868800003
1110	22	0.607900569995806
1110	23	0.8059175168139419
1110	24	0.6583674932876349
1110	25	0.5947477409662767
1111	1	0.1776914860643828
1111	2	0.14794632223842186
1111	3	0.2256768080964946
1111	4	0.4833530395693657
1111	5	0.3439416651012195
1111	6	0.2568803455676232
1111	7	0.15965879966802277
1111	8	0.2053893834316014
1111	9	0.3200274874687494
1111	10	0.2010746520510267
1111	11	0.11091099007794725
1111	12	0.1361542947230916
1111	13	0.15646141312982564
1111	14	-1.8220162851425243
1111	15	-0.579765296630228
1111	16	-0.026703760729134274
1111	17	0.10769935187537527
1111	18	0.16180814294649792
1111	19	0.11797427949944961
1111	20	0.2781748743684602
1111	21	0.35629317491524404
1111	22	0.11462038357614546
1111	23	-1.9405679024485707
1111	24	-0.4630999060661054
1111	25	-0.067988537274978
1112	1	-0.15572177332166603
1112	2	-0.25268608329288966
1112	3	0.012644611194644995
1112	4	0.22148966758048516
1112	5	-0.6230666252280983
1112	6	-0.8323780908407628
1112	7	-0.983558283660507
1112	8	-0.7595645098212742
1112	9	-0.5869892872873308
1112	10	-0.8888086000508011
1112	11	-0.8535058260073285
1112	12	-0.8056371741777042
1112	13	-0.7650912774474605
1112	14	-0.4722012444316448
1112	15	-0.8351167496389138
1112	16	-0.8339426849799167
1112	17	-0.7567387157559509
1112	18	-0.9788669316413632
1112	19	-0.7971205722224468
1112	20	-0.9536183088237138
1112	21	-1.7501709584457004
1112	22	-1.6110708330789123
1112	23	-1.257445920800599
1112	24	-1.405932640004643
1112	25	-1.824106665106875
1113	1	-0.009467306850382329
1113	2	-0.15684826356703852
1113	3	-0.1386340551248324
1113	4	0.1742043417093484
1113	5	-0.05259960707850553
1113	6	-0.1847095068628093
1113	7	-0.03955006254789595
1113	8	-0.022295933587898276
1113	9	0.1618250011533675
1113	10	-0.10713775749937327
1113	11	-0.18247825410420404
1113	12	-0.08528059537123056
1113	13	-0.17844179374645108
1113	14	-0.13178710282193862
1113	15	-0.21270297629205714
1113	16	-0.09045667007791991
1113	17	-0.12545759171656812
1113	18	-0.04464387340802034
1113	19	-0.162756945844869
1113	20	-0.7586332625873666
1113	21	-0.5891665373157906
1113	22	-0.18099553280836744
1113	23	-0.40376860678025395
1113	24	-0.13505713458165758
1113	25	-1.2263807277455427
1114	1	-0.8799118260355954
1114	2	-0.725212132519907
1114	3	-0.5498404761599751
1114	4	-1.4388783660560946
1114	5	-0.6967003520772082
1114	6	-0.5736604738292428
1114	7	-0.5177113963679716
1114	8	-0.47354804223503777
1114	9	-0.4368651668527619
1114	10	-0.5518343748309533
1114	11	-0.4963039376270757
1114	12	-0.6007056078331701
1114	13	-0.587643648800011
1114	14	-0.6423171353310835
1114	15	-1.4300834173120123
1114	16	-0.8575714570759914
1114	17	-0.8282299183596749
1114	18	-0.842224249076132
1114	19	-0.8151386284263751
1114	20	-2.0349231740041063
1114	21	-6.815669135638505
1114	22	-7.84134104518105
1114	23	-7.913113442162164
1114	24	-5.872974532441599
1114	25	-7.406308553487129
1115	1	-1.0368557654696389
1115	2	-1.0281224784299745
1115	3	-0.7931096743334827
1115	4	-0.566012064018544
1115	5	-0.6643971586932835
1115	6	-0.8319190772328469
1115	7	-0.9042426413033553
1115	8	-0.6428626467642999
1115	9	-2.7450535680060923
1115	10	-1.5616571173415892
1115	11	-1.069698898482386
1115	12	-0.9319126732421168
1115	13	-1.364273833979773
1115	14	-1.0766000159196878
1115	15	-1.8914500056382195
1115	16	-1.2980500606560597
1115	17	-2.973348778071731
1115	18	-2.7544417160739982
1115	19	-1.9968612804298616
1115	20	-1.6392866496087422
1115	21	-1.176422385931879
1115	22	-0.854406070552308
1115	23	-0.32601290538835315
1115	24	-1.3566303642552597
1115	25	-1.053980850265141
1116	1	-1.5880995677537904
1116	2	-0.8266625747306797
1116	3	-0.6627141961511853
1116	4	-0.3281112198874325
1116	5	-0.3625063746283175
1116	6	-0.3783292129906499
1116	7	-0.6876000853059736
1116	8	-1.7910767215024137
1116	9	-0.8484384648183578
1116	10	-2.428233175733851
1116	11	-1.0919952241019573
1116	12	-0.7591407222499384
1116	13	-0.7650052930501303
1116	14	-1.183194262090062
1116	15	-1.2014511345951462
1116	16	-0.7972768564341663
1116	17	-0.751500476237851
1116	18	-1.3544923003456688
1116	19	-0.8133222114702363
1116	20	-1.184152982955979
1116	21	-0.6995805209028063
1116	22	-0.3368385769660165
1116	23	-0.1487881965760991
1116	24	-0.25618380879425473
1116	25	-0.2801576954849523
1201	1	0.34388493087877947
1201	2	0.4737451451681345
1201	3	0.3510820973738369
1201	4	0.4042590126443315
1201	5	0.5000907817740483
1201	6	0.4359784744355011
1201	7	0.47708090279030557
1201	8	0.5237035186423142
1201	9	0.7675709434792068
1201	10	0.7618621201848796
1201	11	0.6191620293538591
1201	12	0.6827780726742461
1201	13	0.5854446548203285
1201	14	1.3088124655808144
1201	15	1.0294317461874058
1201	16	0.9775932585163787
1201	17	0.8847581502290183
1201	18	0.8220629953435221
1201	19	0.7738332164950066
1201	20	0.8541183188784913
1201	21	0.9072896426554777
1201	22	1.0247433978448228
1201	23	0.8527180131748366
1201	24	0.8479914383007028
1201	25	0.7538278891704877
1202	1	-0.19407317574153282
1202	2	0.14027303880992648
1202	3	-0.00983636233316787
1202	4	-0.0490956823437827
1202	5	0.11581724932648996
1202	6	0.06703525484926587
1202	7	0.11490087652489933
1202	8	0.16685064604597244
1202	9	0.28991711118061403
1202	10	0.496383133245196
1202	11	0.3112484637569395
1202	12	0.19974955109814374
1202	13	0.32096433772774446
1202	14	0.4738543570611924
1202	15	0.4927591585852369
1202	16	0.5024615569188668
1202	17	0.3837924018243868
1202	18	0.3385604366510222
1202	19	0.4420952365673827
1202	20	0.537218518454182
1202	21	0.6512531479702801
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2211	4	0.33198588428155773
2211	5	0.18588872779423582
2211	6	0.13089312814907572
2211	7	0.12756520943292315
2211	8	0.24620534502078328
2211	9	0.35566143736906497
2211	10	0.3429769449790854
2211	11	0.15493627041557545
2211	12	0.2037565391237166
2211	13	0.1585849966103936
2211	14	0.3770751536387493
2211	15	0.2085062573675387
2211	16	0.10116442316376606
2211	17	0.16469261993812978
2211	18	0.12277431281618334
2211	19	0.25132784702665845
2211	20	0.2844704513967997
2211	21	0.31947753439889226
2211	22	0.38740226746417505
2211	23	0.571219522362405
2211	24	0.45501721681034724
2211	25	0.5967188480487096
2212	1	-0.17409662756198685
2212	2	-0.2072882186416649
2212	3	-0.12475268179576915
2212	4	-0.394683590730871
2212	5	-0.0946228845843109
2212	6	-0.4960752901068801
2212	7	-0.3280795592042054
2212	8	-0.20379755177348358
2212	9	-0.14526183380867508
2212	10	-0.3168791023318036
2212	11	-0.4207626346300515
2212	12	-0.5727672518942271
2212	13	-0.503521387979184
2212	14	-0.3945321913788433
2212	15	-0.5188173133670801
2212	16	-0.3293178044215139
2212	17	-0.5019006852550376
2212	18	-0.33759565838388284
2212	19	-0.5251076647679085
2212	20	-0.4824136166113817
2212	21	-0.4758696638508937
2212	22	-0.3451460494348737
2212	23	-0.0054205763207306745
2212	24	-0.21437554697641303
2212	25	-0.8539788984908014
2213	1	-0.19595835368630787
2213	2	-1.4097902617907394
2213	3	-0.3151542559979177
2213	4	-0.05496216642792007
2213	5	-0.0028900521560402126
2213	6	-0.03864489834972318
2213	7	-0.032343085409564765
2213	8	0.11408560305967796
2213	9	0.12618192327719413
2213	10	-0.35799973469342916
2213	11	-0.11612274702341807
2213	12	-0.039996816214788566
2213	13	-0.35359818059815495
2213	14	-0.06209951911360889
2213	15	-0.22417151195310225
2213	16	-0.07612090442318475
2213	17	0.0558867068552118
2213	18	0.071920296795291
2213	19	-0.047595770444480934
2213	20	0.16513736593078931
2213	21	-0.42530983790175014
2213	22	-0.7148837382593953
2213	23	-0.09475679643598767
2213	24	0.012291504429029487
2213	25	-0.5816129473762075
2214	1	-0.19896394209057533
2214	2	-0.4058091078800956
2214	3	-0.4492652379132558
2214	4	-0.4303044664974607
2214	5	-0.3224425949761951
2214	6	-0.2753026862432506
2214	7	-0.5838836762465647
2214	8	-0.17903898289537068
2214	9	-0.1306598174021545
2214	10	-2.0094284474912314
2214	11	-0.8353479022645018
2214	12	-0.532584277627862
2214	13	-0.29511180525420855
2214	14	-0.8934633041648326
2214	15	-0.5743780877429145
2214	16	-0.8472665410422167
2214	17	-0.7042899339997888
2214	18	-0.5886826901009599
2214	19	-1.5536388338686038
2214	20	-2.5475532321906513
2214	21	-2.0247122387439944
2214	22	-2.1222481720383115
2214	23	-2.5349913829682933
2214	24	-1.6965072772823504
2214	25	-1.874526971714495
2215	1	-0.21824316529780674
2215	2	-0.3668286365371891
2215	3	-0.8611112328148316
2215	4	-0.27465659014024624
2215	5	-0.14854613756818935
2215	6	-0.2285100592916649
2215	7	-0.26057637468113626
2215	8	-0.2513775632369786
2215	9	-0.2562223425948531
2215	10	-0.2735553076487349
2215	11	-0.6484963971018161
2215	12	-0.4950497644332259
2215	13	-0.35523986572798094
2215	14	-0.16918969695739605
2215	15	-0.6569734289333624
2215	16	-0.41475749537204365
2215	17	-0.2767275258488695
2215	18	-0.319761547270069
2215	19	-2.195364937520587
2215	20	-2.3273542283583524
2215	21	-3.1566667397568082
2215	22	-2.263752352088673
2215	23	-3.4105661136856114
2215	24	-2.4859324253301693
2215	25	-1.6695627779290447
2216	1	-1.57163340783233
2216	2	-1.2997332894548776
2216	3	-0.8547325617540764
2216	4	-1.06295628589627
2216	5	-1.9985105029718113
2216	6	-1.579285283298141
2216	7	-1.232082532276749
2216	8	-5.234603665869692
2216	9	-4.199955180673424
2216	10	-2.092460576005287
2216	11	-1.131185799307218
2216	12	-1.4056888169974613
2216	13	-1.9781043816202768
2216	14	-3.5935697907171047
2216	15	-2.1430801687943912
2216	16	-3.1576565860708143
2216	17	-2.7797463049380866
2216	18	-2.70949320488738
2216	19	-1.7765760372274997
2216	20	-1.2268887561044863
2216	21	-0.9784301406546732
2216	22	-1.0224428903053209
2216	23	-1.220055883394238
2216	24	-0.8363910548167297
2216	25	-0.5809830992549578
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	4.0
7	66.0
8	301.0
9	885.0
10	1776.0
11	3502.0
12	6021.0
13	9222.0
14	12313.0
15	15888.0
16	17858.0
17	19370.0
18	19953.0
19	20959.0
20	21215.0
21	22595.0
22	24200.0
23	26111.0
24	28353.0
25	31597.0
26	34576.0
27	38836.0
28	44872.0
29	53338.0
30	65002.0
31	80331.0
32	108681.0
33	156041.0
34	228853.0
35	368260.0
36	649421.0
37	2187720.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.73576124246563	20.02827213019144	12.646448726741763	23.589517900601166
2	15.938350060151391	19.905596057233407	40.16235058138588	23.99370330122932
3	19.21449571745787	24.7637422758094	28.599902169172864	27.42185983755987
4	12.777462840504594	15.725182632590142	36.80579602490208	34.69155850200319
5	14.349714110181027	36.886093211484	34.19023483224772	14.573957846087254
6	33.46990736808696	37.064097262926964	16.010016877069642	13.455978491916435
7	27.903810882792197	31.922207205016807	21.961976559975227	18.21200535221577
8	24.847563777519312	37.38394384213966	19.873211592202654	17.895280788138372
9	24.361163823310157	16.8411055260481	21.12268297699473	37.67504767364701
10	15.656753313983584	29.629938984312744	32.045206284208625	22.66810141749504
11	34.463557478078464	25.08980804345034	21.53189721120432	18.914737267266876
12	21.476508661708255	26.65896830113065	30.63415887174194	21.230364165419154
13	27.25308178079024	23.187192560636312	26.064831984722204	23.494893673851244
14	20.816633930952072	21.885628346569543	27.20379762208886	30.093940100389528
15	23.67798361090559	29.764749952303177	22.880469429825173	23.676797006966062
16	23.185032469776036	30.18319929027405	24.420677818062693	22.21109042188722
17	21.186604727693897	29.42349384387228	27.563230660629124	21.8266707678047
18	21.489430016612303	29.20181851177984	28.072321673700912	21.236429797906943
19	22.16568049539983	27.472183083517756	28.37737290675144	21.98476351433097
20	23.303982366153587	27.323667352396342	27.400957353694587	21.97139292775549
21	23.673547136234788	27.810514750965748	26.82027855807042	21.695659554729037
22	22.197376610802465	27.54323474026556	28.3243928717545	21.934995777177477
23	22.245189599620165	27.773613533785213	28.103803542067908	21.877393324526714
24	22.22753469529632	26.753738488122902	27.990458953467623	23.028267863113157
25	23.053695383729185	26.98036490332872	27.138551091013614	22.82738862192848
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2192.0
1	2192.0
2	2574.5
3	2957.0
4	2957.0
5	2957.0
6	4471.5
7	5986.0
8	5986.0
9	5986.0
10	9609.5
11	13233.0
12	13233.0
13	13233.0
14	22301.5
15	31370.0
16	31370.0
17	31370.0
18	48697.0
19	66024.0
20	66024.0
21	66024.0
22	95413.0
23	124802.0
24	124802.0
25	124802.0
26	165234.5
27	205667.0
28	205667.0
29	205667.0
30	251157.5
31	296648.0
32	296648.0
33	296648.0
34	342473.5
35	388299.0
36	388299.0
37	388299.0
38	432107.5
39	475916.0
40	475916.0
41	475916.0
42	484753.0
43	493590.0
44	493590.0
45	493590.0
46	499032.0
47	504474.0
48	504474.0
49	504474.0
50	487042.0
51	469610.0
52	469610.0
53	469610.0
54	436567.5
55	403525.0
56	403525.0
57	403525.0
58	362549.0
59	321573.0
60	321573.0
61	321573.0
62	278780.0
63	235987.0
64	235987.0
65	235987.0
66	187074.5
67	138162.0
68	138162.0
69	138162.0
70	104146.0
71	70130.0
72	70130.0
73	70130.0
74	50598.5
75	31067.0
76	31067.0
77	31067.0
78	21186.5
79	11306.0
80	11306.0
81	11306.0
82	7613.0
83	3920.0
84	3920.0
85	3920.0
86	2545.5
87	1171.0
88	1171.0
89	1171.0
90	777.5
91	384.0
92	384.0
93	384.0
94	236.0
95	88.0
96	88.0
97	88.0
98	64.0
99	40.0
100	40.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.030478434646209356
2	0.014680833787601604
3	0.021102244445886934
4	0.02254473524593654
5	0.023521906433066914
6	0.01335467289078181
7	0.002047406296844598
8	0.0021870021807203657
9	0.0034666311162482393
10	0.0010702351097142215
11	0.0015122887419874872
12	0.00556056937438476
13	0.0027686516968693994
14	0.0014657567806955643
15	0.003280503271080549
16	0.002884981600099206
17	0.0029547795420370904
18	0.0023498640452420953
19	3.955216709813428E-4
20	5.351175548571108E-4
21	0.08822459860948541
22	0.08375753032546082
23	0.0843159138609639
24	0.0013959588387576804
25	0.23582397982746414
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4298121.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.881505805055646
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.22729297516405	41.65278690455304
2	15.04639367970338	17.117230588744007
3	5.38538090770693	9.189857260925026
4	2.388304586276044	5.434014447540071
5	1.2466422741045498	3.545544487565285
6	0.7342690477258461	2.5059797460414237
7	0.4488936501954232	1.7873622738610508
8	0.2999110185244116	1.3647512272957176
9	0.21438725535207212	1.0975202918854954
>10	0.9276561568522838	9.192435856492704
>50	0.0516561640082058	2.005215386703363
>100	0.026328255033506675	2.678080223207523
>500	0.0016892175575002571	0.6159297557663305
>1k	9.476093553110321E-4	0.9593520157547863
>5k	2.472024405158836E-4	0.853939533664006
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	7596	0.17672838898672236	No Hit
GTACATGGGAAGCAGTGGTATCAAC	6239	0.1451564532501528	No Hit
ATTCCATTCCATTCCATTCCATTCC	6218	0.14466786765658762	No Hit
CCCATGTACTCTGCGTTGATACCAC	5721	0.13310467527554481	No Hit
GAATGGAATGGAATGGAATGGAATG	5506	0.12810248943666314	No Hit
GGTATCAACGCAGAGTACTTTTTTT	5158	0.1200059281718686	No Hit
TATCAACGCAGAGTACTTTTTTTTT	4461	0.10378953966163353	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.326598064596134E-5	0.0
5	0.0	0.0	0.0	2.326598064596134E-5	0.0
6	0.0	0.0	0.0	2.326598064596134E-5	0.0
7	0.0	0.0	0.0	2.326598064596134E-5	0.0
8	0.0	0.0	0.0	2.326598064596134E-5	0.0
9	0.0	0.0	0.0	2.326598064596134E-5	0.0
10	0.0	0.0	0.0	6.979794193788402E-5	0.0
11	0.0	0.0	0.0	6.979794193788402E-5	0.0
12	0.0	0.0	0.0	6.979794193788402E-5	0.0
13	0.0	0.0	0.0	9.306392258384537E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGACCG	60	2.5767336E-5	14.246246	5
TAGTCCG	95	7.424023E-8	12.996576	5
TAAACCG	75	1.4840347E-5	12.66333	5
CTACGAC	60	0.005888014	11.080931	3
CGTGCGA	60	0.0058972	11.07861	10
ACGTCGC	70	0.0014970659	10.853021	6
GTCGTAC	90	9.519345E-5	10.555969	1
CTATCGC	75	0.0026588393	10.128897	9
TACGACC	160	6.21003E-9	10.091091	4
AACCGCT	95	1.6526428E-4	9.995623	7
GTCTTAG	295	0.0	9.983442	1
TACCGTG	125	2.5873815E-6	9.875674	7
TATTCCG	185	6.530172E-10	9.754187	5
GTCCTAC	270	0.0	9.500373	1
GTACGAC	160	6.667506E-8	9.49794	3
TAGACTG	500	0.0	9.4974985	5
ACTATCG	615	0.0	9.418749	11
TATCGGT	605	0.0	9.416705	13
ATCGCGC	255	0.0	9.309648	8
CTATCGG	635	0.0	9.271638	12
>>END_MODULE