FastQCFastQC Report
Wed 25 May 2016
SRR1294704_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294704_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4298121
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT190290.4427283457119983No Hit
GGTATCAACGCAGAGTACTTTTTTT137640.3202329576110119No Hit
TATCAACGCAGAGTACTTTTTTTTT119970.2791219698095982No Hit
ACGCAGAGTACTTTTTTTTTTTTTT74090.17237765060592755No Hit
ATTCCATTCCATTCCATTCCATTCC71140.16551418631536896No Hit
GTACATGGGAAGCAGTGGTATCAAC64510.15008884114709659No Hit
CCCATGTACTCTGCGTTGATACCAC61500.14308578097266222No Hit
GAATGGAATGGAATGGAATGGAATG58720.136617838353085No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAC1058.54925E-1114.5039941
CGCCCTA2750.010.70257316
TCGTACC1201.4965208E-610.3071062
TAGACAG7400.010.0285365
CCGGTAC951.6206547E-410.0150013
CTAGACA5250.09.6048634
GTACGAC1401.0384301E-69.5142523
GGACCGT1304.2686315E-69.49554356
ATTAGAC2750.09.3412653
GACAGCG2650.09.31551557
ACTATCG5750.09.24654311
CGTACCC1554.1743078E-79.207343
GTCCTGG10250.09.19323351
ATCGGTC6000.09.17774414
AGCGTAA1151.1187546E-49.081532510
TATACTG14800.09.0642545
CTATCGG6000.09.01950712
TATCGGT6000.09.01950713
GTAGGAC2553.6379788E-128.9545913
CCGTACA3400.08.93573617
Produced by FastQC (version 0.11.5)