FastQCFastQC Report
Wed 25 May 2016
SRR1294703_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294703_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2558561
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC57750.22571281278812583No Hit
CCCATGTACTCTGCGTTGATACCAC52830.2064832536726699No Hit
GTATCAACGCAGAGTACTTTTTTTT42330.16544456043846523No Hit
GGTATCAACGCAGAGTACTTTTTTT33200.12976043955958055No Hit
GAGTACATGGGAAGCAGTGGTATCA27130.10603616642323556No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATCG905.43836E-712.66111611
TATCGGT951.3682562E-510.99474913
GGACAGT2700.010.9022026
CTATCGG1053.4867244E-610.852385512
CGTGTTA803.7884607E-410.68260910
CTAGACT3300.010.3605134
GTCCTAT2350.010.107091
GCTAGAC1701.5679689E-910.0561853
AATGCCG800.00453056869.496585
AAACCGA900.00111890579.4954666
CCAGGAC3950.09.3772873
CCTACAC3350.09.3557543
GTCTAGA2700.09.1487881
GTCTAAG2501.8189894E-129.1206381
ATCGGTC1252.7707252E-59.11564714
TACCGTG1054.515467E-49.0433027
GGTTAAC950.00182313539.0006291
TCTGTCG1901.0670192E-88.9957048
GTTCTAC2054.138201E-98.8054941
CTGCGCT1304.3938264E-58.7652179
Produced by FastQC (version 0.11.5)