FastQCFastQC Report
Wed 25 May 2016
SRR1294703_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294703_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2558561
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT110610.43231331987003635No Hit
GGTATCAACGCAGAGTACTTTTTTT91920.35926444591315193No Hit
TATCAACGCAGAGTACTTTTTTTTT69620.27210607837765055No Hit
GTACATGGGAAGCAGTGGTATCAAC63340.2475610313766215No Hit
CCCATGTACTCTGCGTTGATACCAC58020.2267680934712911No Hit
ACGCAGAGTACTTTTTTTTTTTTTT47190.18443961273543996No Hit
GAGTACATGGGAAGCAGTGGTATCA33070.12925234145287134No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTTA250.006054238418.98850815
GTACCGT600.005894511.0790136
CGCACGA750.002661896210.12720512
TATTCCG800.0044612939.5143355
CTAGGAC2500.09.5143353
GCACCGT800.00453168769.4962966
AACCGTG800.00453753639.4948117
TCAGTAC3150.09.3633153
TATACTG6350.09.2895875
CGGGTTC1856.7720975E-99.23765318
CGTACAC1651.0418262E-79.2260223
GTATAGG2900.09.1889541
TAGGACT2900.09.1862554
TAACACG1252.7066331E-59.1337614
TTAGTAC2106.493792E-109.0612723
ACTGCGG1054.5185795E-49.0426788
TCTACAC2950.09.0305563
CGTAGAC850.007346078.9546693
TAGGACC1701.6472768E-78.9546694
TTCTAGG4250.08.9546682
Produced by FastQC (version 0.11.5)