FastQCFastQC Report
Wed 25 May 2016
SRR1294702_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294702_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3870685
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC1241993.20870853608599No Hit
CCCATGTACTCTGCGTTGATACCAC1184123.0592001157417874No Hit
GAGTACATGGGAAGCAGTGGTATCA575341.4864035693940478No Hit
CATGTACTCTGCGTTGATACCACTG460751.1903577790494448No Hit
GCGTTGATACCACTGCTTCCCATGT388751.0043441923070464No Hit
GTATCAACGCAGAGTACATGGGAAG336160.868476768323953No Hit
GCTTCCCATGTACTCTGCGTTGATA327820.8469301945262918No Hit
ACGCAGAGTACATGGGAAGCAGTGG314960.813706101116469No Hit
ACTCTGCGTTGATACCACTGCTTCC245750.6349005408603386No Hit
TATCAACGCAGAGTACATGGGAAGC235290.6078769003419291No Hit
GGTATCAACGCAGAGTACATGGGAA200950.5191587535539575No Hit
CAGTGGTATCAACGCAGAGTACATG179930.4648531203133296No Hit
GTGGTATCAACGCAGAGTACATGGG164590.4252218922490464No Hit
GCAGTGGTATCAACGCAGAGTACAT148050.3824904377390565No Hit
GTACATGGTAAGCAGTGGTATCAAC140320.3625198123846296No Hit
ATACCACTGCTTCCCATGTACTCTG130790.3378988473616427No Hit
GTACTCTGCGTTGATACCACTGCTT125940.32536876547691174No Hit
CATGGGAAGCAGTGGTATCAACGCA123760.319736687433878No Hit
CTGCTTCCCATGTACTCTGCGTTGA118450.3060181854116261No Hit
GGGAAGCAGTGGTATCAACGCAGAG115360.2980351023139315No Hit
ACATGGGAAGCAGTGGTATCAACGC109580.28310234493377784No Hit
TACCACTGCTTCCCATGTACTCTGC100260.25902391953878967No Hit
GCAGAGTACATGGGAAGCAGTGGTA99540.25716378367136566No Hit
ACCATGTACTCTGCGTTGATACCAC99050.25589785787270214No Hit
AAGCAGTGGTATCAACGCAGAGTAC97500.2518933987136644No Hit
CCATGTACTCTGCGTTGATACCACT95280.2461579797891071No Hit
GATACCACTGCTTCCCATGTACTCT94430.24396198605673153No Hit
CTTCCCATGTACTCTGCGTTGATAC93530.2416368162224516No Hit
CCACTGCTTCCCATGTACTCTGCGT91510.23641810170551206No Hit
GTTGATACCACTGCTTCCCATGTAC90730.23440295451580279No Hit
GTACATGGGTAAGCAGTGGTATCAA90270.23321453437828188No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA83720.2162924650287998No Hit
ATGGGAAGCAGTGGTATCAACGCAG80230.2072759731158697No Hit
GGTAAGCAGTGGTATCAACGCAGAG69510.17958061686755702No Hit
TTCCCATGTACTCTGCGTTGATACC67200.17361268095957175No Hit
TGATACCACTGCTTCCCATGTACTC64160.1657587739637816No Hit
CCCCATGTACTCTGCGTTGATACCA61950.1600491902596052No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA55890.1443930467087867No Hit
ATCAACGCAGAGTACATGGGAAGCA53030.13700417367985254No Hit
GCTTACCATGTACTCTGCGTTGATA52310.13514403781242856No Hit
GAGTACATGGTAAGCAGTGGTATCA48260.12468077355816864No Hit
AAAAAGTACTCTGCGTTGATACCAC41190.10641527274888037No Hit
GTATCAACGCAGAGTACTTTTTTTT39300.10153241609689241No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGGTT551.9690045E-413.811837
CTGATCG500.001505458713.2938859
GTACAAA9350.012.9066511
TATCGAC1301.9099389E-1012.45405319
GTCCTAC2500.011.4025671
GTGTACG600.005868493611.085831
GGTATCG1658.0035534E-1110.94539517
ACCGTGT1158.8799425E-710.7341948
GTATCGA1553.6361598E-910.42507218
CGAATTA750.002643018310.13613915
CTAGCAC2350.010.1064383
GAACAAA16300.010.0267981
GTCCTAG2700.09.8540711
GTCCTGA3850.09.6255441
GTACAAG4800.09.502141
GTCTTAG2700.09.502141
CCGGTAC900.00111424769.50005153
GGGTAAG25000.09.457657
ACGCAAA4150.09.3876561
TGGGTAA24100.09.2988696
Produced by FastQC (version 0.11.5)