##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294702_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3870685
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.83410894970787	33.0	33.0	33.0	27.0	33.0
2	30.823283217311666	33.0	33.0	33.0	27.0	33.0
3	30.802752226027177	33.0	33.0	33.0	27.0	33.0
4	34.03277946926707	37.0	33.0	37.0	27.0	37.0
5	34.53093987239985	37.0	37.0	37.0	27.0	37.0
6	34.64686069778347	37.0	37.0	37.0	27.0	37.0
7	34.67985847466275	37.0	37.0	37.0	27.0	37.0
8	34.580837241986885	37.0	37.0	37.0	27.0	37.0
9	34.25308001038576	37.0	37.0	37.0	27.0	37.0
10	34.36583653797713	37.0	37.0	37.0	27.0	37.0
11	34.64321018114365	37.0	37.0	37.0	27.0	37.0
12	34.57533537345457	37.0	37.0	37.0	27.0	37.0
13	34.42654284706712	37.0	37.0	37.0	27.0	37.0
14	35.619674812081065	37.0	37.0	40.0	27.0	40.0
15	35.850149779690156	40.0	37.0	40.0	27.0	40.0
16	35.85227085128343	37.0	37.0	40.0	27.0	40.0
17	35.86448936041037	37.0	37.0	40.0	27.0	40.0
18	35.848356298691314	37.0	37.0	40.0	27.0	40.0
19	35.827310153112435	40.0	37.0	40.0	27.0	40.0
20	35.74190227311187	37.0	37.0	40.0	27.0	40.0
21	35.6528319922701	37.0	37.0	40.0	27.0	40.0
22	35.59009090122291	37.0	37.0	40.0	27.0	40.0
23	35.24580868760956	37.0	33.0	40.0	27.0	40.0
24	35.58435160701529	37.0	37.0	40.0	27.0	40.0
25	34.475014370841336	37.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.5341346636709972
1101	2	0.6311235135326889
1101	3	0.32365597158380055
1101	4	0.5226896006681514
1101	5	0.4702685215241118
1101	6	0.3540754897210263
1101	7	0.4413270809388692
1101	8	0.4659714963050945
1101	9	0.7096769589460408
1101	10	0.7646792837295067
1101	11	0.5413985470758433
1101	12	0.6053891339351907
1101	13	0.5157031349266461
1101	14	1.1757605944091694
1101	15	0.9449922153134267
1101	16	0.9192287996561603
1101	17	0.9038784350344429
1101	18	1.0614553076898616
1101	19	0.9611340857805857
1101	20	1.0879494159385814
1101	21	0.9526797952698303
1101	22	1.015974226913869
1101	23	1.355973761618337
1101	24	1.027257001801111
1101	25	0.9604832231355118
1102	1	0.2818069114788706
1102	2	0.5041197017043118
1102	3	0.27070147935056355
1102	4	0.19705960821374902
1102	5	0.20311074045055477
1102	6	0.11399404929223067
1102	7	0.28074698050434677
1102	8	0.33176781717517656
1102	9	0.5205302620969761
1102	10	0.5509684102613974
1102	11	0.27580387582977295
1102	12	0.3795461084102456
1102	13	0.46309697615684087
1102	14	1.0587094924915448
1102	15	0.8538605706330742
1102	16	0.5765623411439407
1102	17	0.7260098065120815
1102	18	0.7206863070914125
1102	19	0.6566610417819376
1102	20	0.834524299502192
1102	21	0.8100683025422271
1102	22	0.840281462882956
1102	23	1.1390735716774003
1102	24	0.974738658190148
1102	25	0.906968665333622
1103	1	0.23367262699022362
1103	2	0.45617321213471484
1103	3	0.09195916511977842
1103	4	-0.10718182423886446
1103	5	0.11526339134584163
1103	6	0.051480902059104494
1103	7	0.07695168014369358
1103	8	0.22258429609988184
1103	9	0.4028057264207092
1103	10	0.5576419616825703
1103	11	0.32087783398105785
1103	12	0.24293626744807995
1103	13	0.25343014531525654
1103	14	0.8789227080426159
1103	15	0.6387677058867283
1103	16	0.4449463833421916
1103	17	0.4015256070236717
1103	18	0.5799656321736322
1103	19	0.6030139754817512
1103	20	0.7248870201453315
1103	21	0.6689453461227188
1103	22	0.5496410218349936
1103	23	1.0899297703396442
1103	24	0.7876869748676754
1103	25	0.658784492854636
1104	1	0.2046686293136588
1104	2	0.3887746217272756
1104	3	0.1875090397846968
1104	4	0.18952318326260098
1104	5	0.15791241895061603
1104	6	0.06890474585463124
1104	7	0.03664211647129889
1104	8	0.25598350153448735
1104	9	0.3900231012487012
1104	10	0.44109282318652987
1104	11	0.24605108216315585
1104	12	0.2064058903867263
1104	13	0.18577367836675762
1104	14	0.6451481496662907
1104	15	0.4909543759494994
1104	16	0.49140820661817486
1104	17	0.45680356513349096
1104	18	0.3795289007634537
1104	19	0.45628029777462586
1104	20	0.5565107850434288
1104	21	0.5577777574330085
1104	22	0.6486370987071766
1104	23	1.0167394256448716
1104	24	0.8326629794896974
1104	25	0.6653426703862593
1105	1	0.2566308019654855
1105	2	0.297026357565219
1105	3	0.3390013292080205
1105	4	0.2616846864585938
1105	5	0.31183145217999453
1105	6	0.25269511930017785
1105	7	0.25854157975007297
1105	8	0.26907070427686364
1105	9	-1.4749258154243492
1105	10	0.030760636992269497
1105	11	0.1134973461611466
1105	12	0.1616136731807174
1105	13	0.22866161780641647
1105	14	-1.4613636465006792
1105	15	-0.11626143816286572
1105	16	-0.09095853092097883
1105	17	0.24654763073962016
1105	18	0.22793911124262678
1105	19	0.4181765872571219
1105	20	0.51280384478477
1105	21	0.6711374225168072
1105	22	0.6239884016446879
1105	23	1.100119491783559
1105	24	0.774136024411014
1105	25	0.3723784119047622
1106	1	0.06532148996652154
1106	2	-0.028389295436642925
1106	3	0.05641040868606595
1106	4	-1.219392225652804
1106	5	-0.25170499066678786
1106	6	-0.09992642297653731
1106	7	-0.06111148128230326
1106	8	0.018882764433747923
1106	9	0.3226310338566378
1106	10	0.3166631987568991
1106	11	0.24166357777565395
1106	12	0.03581971980055698
1106	13	0.11171260430567997
1106	14	0.49702600154414256
1106	15	0.3265107115058399
1106	16	0.11460932981929517
1106	17	-0.02038141205149202
1106	18	0.13874265997721125
1106	19	0.10563094712527032
1106	20	0.3130920416246852
1106	21	0.4493186489739003
1106	22	0.4187318688019488
1106	23	0.67521608412153
1106	24	0.4107605575872739
1106	25	0.4391229441665345
1107	1	0.35246030097167846
1107	2	0.31752099200109285
1107	3	0.2984873506436756
1107	4	0.39619579208318356
1107	5	0.38743586267454333
1107	6	0.25015656612792725
1107	7	0.13609291401278512
1107	8	0.23498156167813278
1107	9	0.4657516364044483
1107	10	0.3810986396839908
1107	11	0.2006995051886733
1107	12	0.20202103874650845
1107	13	0.26559980055564836
1107	14	0.7342392011678385
1107	15	0.41006303610828354
1107	16	0.4363364749354588
1107	17	0.4549774976749603
1107	18	0.5296931756293972
1107	19	0.45946387375056474
1107	20	0.5660334496973647
1107	21	0.6303418443169022
1107	22	0.5861551269173262
1107	23	0.9134596666371877
1107	24	0.8343507550438218
1107	25	0.6675056367497092
1108	1	0.49323786471248354
1108	2	0.44095039500608735
1108	3	0.5028874918870585
1108	4	0.6859496565715091
1108	5	0.548792634632683
1108	6	0.35593829675872257
1108	7	0.3133643945145792
1108	8	0.44852179975310236
1108	9	0.2425130012947463
1108	10	0.5771426689457542
1108	11	0.3647003869088934
1108	12	0.36519471661280534
1108	13	0.2815989766359124
1108	14	-0.9089435198481013
1108	15	0.059426337420141806
1108	16	0.4149699977493171
1108	17	0.47622206836969383
1108	18	0.6323381786718016
1108	19	0.5460734440406867
1108	20	0.6775869535106196
1108	21	0.6434411434596683
1108	22	0.7784304327607501
1108	23	1.1457364595296227
1108	24	0.9085827441478997
1108	25	0.8257250242956999
1109	1	0.44117439850887763
1109	2	0.3467951941609222
1109	3	0.4412532898142345
1109	4	0.620118644318687
1109	5	0.6121738628108773
1109	6	0.36171558992847963
1109	7	0.3931067322226909
1109	8	0.4395977713300354
1109	9	0.5194613621877053
1109	10	0.5282769178000422
1109	11	0.3790588897623479
1109	12	0.3660270232619425
1109	13	0.37707095584531203
1109	14	0.7244137876243499
1109	15	0.45451524292790424
1109	16	0.4264758942861562
1109	17	0.49135052984389915
1109	18	0.4914247645166725
1109	19	0.49350563217677745
1109	20	0.6814731685496511
1109	21	0.6328191058770329
1109	22	0.7141387652489612
1109	23	1.1547421646877325
1109	24	0.8462528994689151
1109	25	0.7074574390983557
1110	1	0.33302803240719214
1110	2	0.22862236924502
1110	3	0.3658017211389293
1110	4	0.63442432909833
1110	5	0.4967486225950708
1110	6	0.29325052836992427
1110	7	0.23036422124722122
1110	8	0.2753142548323666
1110	9	0.44802916134950976
1110	10	0.472247695785164
1110	11	0.2574522024911232
1110	12	0.18556084714400356
1110	13	0.2203786593139867
1110	14	0.6566030003090191
1110	15	0.4534039557049141
1110	16	0.29551822503692193
1110	17	0.3850141522225812
1110	18	0.5240748156928632
1110	19	0.4425469424363726
1110	20	0.5152957310987603
1110	21	0.5833940526271277
1110	22	0.7220020541217593
1110	23	0.9190418770933135
1110	24	0.7432942518676668
1110	25	0.8274951610917682
1111	1	0.21008522692097387
1111	2	0.23556480330281815
1111	3	0.32011875714017535
1111	4	0.7259022318387096
1111	5	0.5006895873257591
1111	6	0.33474672942194417
1111	7	0.3597496710852681
1111	8	0.4384505308255342
1111	9	0.38592960760982464
1111	10	0.21474402246737867
1111	11	0.28805681148078577
1111	12	0.12277578949066736
1111	13	0.1786940129629997
1111	14	-1.371478080329183
1111	15	-0.34042323176265654
1111	16	0.21073149595572005
1111	17	0.281814405667852
1111	18	0.37275870280047485
1111	19	0.2323348458868324
1111	20	0.4345127818479
1111	21	0.5528686452848675
1111	22	0.3604738446798308
1111	23	-1.7114278159866458
1111	24	-0.32501307237295407
1111	25	0.13744398044099881
1112	1	-0.14771700451463943
1112	2	-0.3171812721139453
1112	3	0.06492011140045406
1112	4	0.13678094433047505
1112	5	-0.7083216069628122
1112	6	-0.8439686139977667
1112	7	-0.9012295230033018
1112	8	-0.6713652145010371
1112	9	-0.6260554522836088
1112	10	-0.8419845237009298
1112	11	-0.8780858075409341
1112	12	-0.843832170948744
1112	13	-0.955566231931229
1112	14	-0.668972603110447
1112	15	-0.9322332980088248
1112	16	-0.9397456280498417
1112	17	-0.9131898649709171
1112	18	-1.054841445290002
1112	19	-0.8394379848425473
1112	20	-0.9140031296751658
1112	21	-1.721059236878709
1112	22	-1.74809857638855
1112	23	-1.4579096172840735
1112	24	-1.688792025165064
1112	25	-1.993140858781608
1113	1	0.008165636411774813
1113	2	-0.12964652273264932
1113	3	0.0283236285217221
1113	4	0.22297781644022763
1113	5	0.06662024178586279
1113	6	-0.013723966967987167
1113	7	0.03200213814710651
1113	8	0.06496449749609212
1113	9	0.2538226492587796
1113	10	-0.042755110799909346
1113	11	-0.20816854380161942
1113	12	-0.07659562391672381
1113	13	-0.16222836908127647
1113	14	-0.05569891821679107
1113	15	-0.2361034729920064
1113	16	-0.14989347207962567
1113	17	-0.11244732406199631
1113	18	-0.04287679449542736
1113	19	-0.15833260631196566
1113	20	-0.6829622023519946
1113	21	-0.5615842926769616
1113	22	-0.13327570952142054
1113	23	-0.2622321746563685
1113	24	-0.10635819083341147
1113	25	-1.2327959798299517
1114	1	-1.0372870222750201
1114	2	-0.8249910324053502
1114	3	-0.5212848607523242
1114	4	-1.4602897238897512
1114	5	-0.819762724320853
1114	6	-0.7168991147198724
1114	7	-0.5646661380479685
1114	8	-0.5737373190120181
1114	9	-0.5399806663697433
1114	10	-0.47916462000669924
1114	11	-0.5760924780537948
1114	12	-0.6135544905828283
1114	13	-0.6363660712959316
1114	14	-0.678563963495364
1114	15	-1.4866142864815473
1114	16	-0.9303590211123591
1114	17	-1.019738770124441
1114	18	-0.9762358238086151
1114	19	-0.8759703551182341
1114	20	-2.0990519801523604
1114	21	-6.845922451771788
1114	22	-7.524731796221349
1114	23	-7.94875632746556
1114	24	-5.874509718049975
1114	25	-7.269509464007157
1115	1	-1.0109763035726544
1115	2	-1.0575713435842076
1115	3	-0.7700199177442606
1115	4	-0.5501404615734558
1115	5	-0.6241490424211875
1115	6	-0.866827695050226
1115	7	-1.0313399946142638
1115	8	-0.6580801142532664
1115	9	-2.6728011201962083
1115	10	-1.5323358450697242
1115	11	-1.1330892090460978
1115	12	-0.9461884863455836
1115	13	-1.3639019488950765
1115	14	-0.9994688363300099
1115	15	-2.067323785776935
1115	16	-1.357764521864901
1115	17	-3.0121601365888324
1115	18	-2.87488220537179
1115	19	-2.134435742941413
1115	20	-1.80508190930788
1115	21	-1.3914191779708247
1115	22	-0.9730335419289773
1115	23	-0.3482434479586871
1115	24	-1.2601203322591346
1115	25	-0.9439225285341095
1116	1	-1.6980613622415461
1116	2	-0.9920899812003654
1116	3	-0.6960857334948898
1116	4	-0.4281680948871269
1116	5	-0.3989274559408287
1116	6	-0.517918336670455
1116	7	-0.8128930511154451
1116	8	-1.6679923479427927
1116	9	-0.9778585116185852
1116	10	-2.541998871939551
1116	11	-1.1575702111429464
1116	12	-0.8711425023479151
1116	13	-0.9729578772129344
1116	14	-1.4470814110446497
1116	15	-1.4098364532080865
1116	16	-0.9554192631319367
1116	17	-0.7496488963332979
1116	18	-1.3876930527049183
1116	19	-1.0746096887027576
1116	20	-1.2692819075114414
1116	21	-0.7400857667915517
1116	22	-0.376409810293886
1116	23	-0.26993566905314736
1116	24	-0.24569972064951173
1116	25	-0.42011524315188353
1201	1	0.28088600528072405
1201	2	0.45507481005499884
1201	3	0.22904547243502194
1201	4	0.21498564489527894
1201	5	0.2574023482277852
1201	6	0.2549568968629785
1201	7	0.2899969374105993
1201	8	0.42446762371072566
1201	9	0.765400404661051
1201	10	0.5516952742145094
1201	11	0.4594874107434137
1201	12	0.4794244187244061
1201	13	0.480998360324854
1201	14	1.1165288813724246
1201	15	0.9307349721756566
1201	16	0.8812613092372317
1201	17	0.7463595255279998
1201	18	0.6273874252177194
1201	19	0.6606270790926203
1201	20	0.7967597624638714
1201	21	0.7938696430868433
1201	22	0.8926756938096005
1201	23	0.6982988600203299
1201	24	0.8176218017451404
1201	25	0.7723639513750058
1202	1	-0.2634894528294396
1202	2	0.07671978202348839
1202	3	-0.028638929841690697
1202	4	-0.11064353877354449
1202	5	-0.0027672269234599867
1202	6	-0.09302337433249619
1202	7	-0.15465251131994506
1202	8	0.07281328589905911
1202	9	0.15594506196195823
1202	10	0.3568313257962359
1202	11	0.06337086452040097
1202	12	0.1196405570998067
1202	13	0.2772821496469007
1202	14	0.37693855073960236
1202	15	0.3462484392350049
1202	16	0.32612823969710547
1202	17	0.20653048485684167
1202	18	0.23966020720228443
1202	19	0.26288693081721703
1202	20	0.2961274420700093
1202	21	0.3355490166527133
1202	22	0.27496692107695964
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2211	6	0.2027073085412212
2211	7	0.17406681844742167
2211	8	0.3036216224767685
2211	9	0.4289568743181462
2211	10	0.3767503113604249
2211	11	0.16910059164592894
2211	12	0.28800156716840064
2211	13	0.10883125240901137
2211	14	0.5668086347841026
2211	15	0.32033556309874456
2211	16	0.23028660304580484
2211	17	0.3412607012317608
2211	18	0.27733787515885666
2211	19	0.2772539896454731
2211	20	0.4638125140081826
2211	21	0.4445528783437993
2211	22	0.5207243526325058
2211	23	0.6032644684062376
2211	24	0.5055037704924104
2211	25	0.6552665793473693
2212	1	-0.20583063485640452
2212	2	-0.2843555659855497
2212	3	-0.07980567338989175
2212	4	-0.4277848876157009
2212	5	-0.16207040874256506
2212	6	-0.4198538295616743
2212	7	-0.46889181648469247
2212	8	-0.2960716272889954
2212	9	-0.10241827151449456
2212	10	-0.49205161163407496
2212	11	-0.4472759222600544
2212	12	-0.5447475099453527
2212	13	-0.5222293885032201
2212	14	-0.3430818072193844
2212	15	-0.5897213426745935
2212	16	-0.4154372062614371
2212	17	-0.39487719469343574
2212	18	-0.31718755743473537
2212	19	-0.41264407500492695
2212	20	-0.4887711430745654
2212	21	-0.337720212989538
2212	22	-0.4295285496897847
2212	23	0.01696005107501719
2212	24	-0.13284061658715274
2212	25	-0.7438008358071926
2213	1	-0.06525586192230648
2213	2	-1.2033367039906082
2213	3	-0.17719273885150955
2213	4	0.1214610824136173
2213	5	0.12542480039742543
2213	6	0.12708438600148497
2213	7	0.07803479720823958
2213	8	0.2414618476685746
2213	9	0.16181325418639858
2213	10	-0.24454565753391933
2213	11	0.06502648720413617
2213	12	0.11078695870222077
2213	13	-0.22968670430674365
2213	14	0.06319558820553084
2213	15	-0.06664191525334928
2213	16	0.038031101601724515
2213	17	0.18490520307118885
2213	18	0.13390521661517596
2213	19	0.19414238809572737
2213	20	0.2954746264662873
2213	21	-0.3039854854777744
2213	22	-0.7344085507319136
2213	23	0.025866979854392014
2213	24	0.2440480712026556
2213	25	-0.3404070648716697
2214	1	-0.36638836770270444
2214	2	-0.4608633513869975
2214	3	-0.47928183716419426
2214	4	-0.5945532592788467
2214	5	-0.38015268435150773
2214	6	-0.36055303123294635
2214	7	-0.5911335792502257
2214	8	-0.18641015711199316
2214	9	-0.11587756063389776
2214	10	-1.954856490144273
2214	11	-0.8781879350781168
2214	12	-0.5522898990088336
2214	13	-0.45380278649187744
2214	14	-1.0426636866782815
2214	15	-0.8023476446662912
2214	16	-0.9848605777770203
2214	17	-0.6727785807014754
2214	18	-0.7759224199248891
2214	19	-1.517208162395555
2214	20	-2.595647682239189
2214	21	-2.0409673568877267
2214	22	-2.2172380608474427
2214	23	-2.681124252579977
2214	24	-1.7189975374078443
2214	25	-2.080556950908395
2215	1	-0.22185387257875533
2215	2	-0.2738705446170293
2215	3	-0.6878542412505944
2215	4	-0.2667332356941827
2215	5	-0.21571988069894132
2215	6	-0.17118809611860542
2215	7	-0.09974986825607601
2215	8	-0.20804832177600474
2215	9	-0.12682610579778952
2215	10	-0.12558501691918167
2215	11	-0.5366863007591789
2215	12	-0.49904178870460214
2215	13	-0.3367314600446676
2215	14	-0.33481723936198904
2215	15	-0.7058152955427346
2215	16	-0.45870370449867437
2215	17	-0.29312204746166515
2215	18	-0.2758648731568343
2215	19	-2.1880816346575926
2215	20	-2.375316323263334
2215	21	-3.1896165735001674
2215	22	-2.4179506109138273
2215	23	-3.4902770648796313
2215	24	-2.380068227384548
2215	25	-1.7141642866484403
2216	1	-1.6337284625653261
2216	2	-1.311913875618604
2216	3	-0.9309185981415489
2216	4	-0.9904364674491362
2216	5	-1.891714913406517
2216	6	-1.5853826131681075
2216	7	-1.2151512606702681
2216	8	-5.158790658365021
2216	9	-4.200485125749772
2216	10	-2.160217436898108
2216	11	-1.2557580742420953
2216	12	-1.315436630063033
2216	13	-2.1007665608197215
2216	14	-3.714490438384555
2216	15	-2.2569275362778143
2216	16	-3.332069089003973
2216	17	-2.9216369525827943
2216	18	-2.8936746830250897
2216	19	-1.921539369738639
2216	20	-1.1008477998917527
2216	21	-1.0029684425889585
2216	22	-0.8858128090017843
2216	23	-1.3068621486530816
2216	24	-0.6923053560635992
2216	25	-0.4968973832186947
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	1.0
5	1.0
6	12.0
7	159.0
8	653.0
9	1562.0
10	2595.0
11	4342.0
12	7005.0
13	10173.0
14	13849.0
15	18925.0
16	23709.0
17	25815.0
18	26533.0
19	27279.0
20	27543.0
21	29364.0
22	30339.0
23	31518.0
24	33295.0
25	35401.0
26	37193.0
27	41036.0
28	46000.0
29	53797.0
30	63881.0
31	77989.0
32	103225.0
33	145996.0
34	210883.0
35	332485.0
36	578286.0
37	1829841.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.42163808143554	19.19559410048587	11.274237060280308	24.10853075779828
2	13.168587532489951	22.679127854651746	37.18276515273861	26.969519460119695
3	21.160160845895813	28.69493468768727	26.916182295116197	23.228722171300724
4	13.36337089104817	19.21663737854042	33.70994418027703	33.710047550134384
5	11.916468170850175	40.36481019992407	34.48568155370215	13.2330400755236
6	32.3901122113678	33.860381638027064	18.153709962024305	15.595796188580833
7	24.75928075145124	33.9407422947621	22.545939990967536	18.75403696281912
8	26.492217153395465	36.19435116764014	21.16042401767834	16.15300766128605
9	26.516913502226352	18.209576231030024	20.30366948821044	34.96984077853318
10	16.062161306782585	32.065468308972186	31.85955575076055	20.012814633484687
11	29.536826571163864	27.88915005986149	19.961856062700324	22.612167306274323
12	23.240129122737393	30.957444994426968	28.468029100672638	17.334396782162997
13	28.259349177810773	23.125907970922192	23.18006004783261	25.434682803434423
14	21.28417105504856	26.56958014921058	25.764518863432556	26.3817299323083
15	29.54758131481274	26.296693978722534	21.747162048833065	22.40856265763166
16	19.482538704057703	29.84711112443093	29.940481926652506	20.729868244858856
17	24.650485650101984	30.064499294034363	27.20407896997482	18.080936085888826
18	24.917331745978842	27.050701042812324	28.288754869734362	19.743212341474468
19	22.65557020707558	31.152096901203514	28.72517480204947	17.467158089671436
20	23.357385136899968	28.255241401172356	27.284428315179298	21.102945146748382
21	19.781579129729668	30.01145990738988	29.637952772356385	20.569008190524062
22	21.597131149236503	29.362203527265358	23.65302192233066	25.38764340116748
23	18.71991543742721	30.482837190156154	27.302328780441385	23.494918591975246
24	20.87333315765188	34.26157727839465	24.97487496906198	19.890214594891493
25	22.235469880840995	27.31435286842166	23.116272288415914	27.33390496232143
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	11429.0
1	11429.0
2	13960.5
3	16492.0
4	16492.0
5	16492.0
6	19833.5
7	23175.0
8	23175.0
9	23175.0
10	23999.5
11	24824.0
12	24824.0
13	24824.0
14	30015.0
15	35206.0
16	35206.0
17	35206.0
18	47986.5
19	60767.0
20	60767.0
21	60767.0
22	81574.0
23	102381.0
24	102381.0
25	102381.0
26	129204.5
27	156028.0
28	156028.0
29	156028.0
30	185047.0
31	214066.0
32	214066.0
33	214066.0
34	238469.0
35	262872.0
36	262872.0
37	262872.0
38	286329.0
39	309786.0
40	309786.0
41	309786.0
42	355315.5
43	400845.0
44	400845.0
45	400845.0
46	633607.0
47	866369.0
48	866369.0
49	866369.0
50	750430.5
51	634492.0
52	634492.0
53	634492.0
54	473311.5
55	312131.0
56	312131.0
57	312131.0
58	248923.5
59	185716.0
60	185716.0
61	185716.0
62	155272.0
63	124828.0
64	124828.0
65	124828.0
66	98091.5
67	71355.0
68	71355.0
69	71355.0
70	53060.0
71	34765.0
72	34765.0
73	34765.0
74	24910.5
75	15056.0
76	15056.0
77	15056.0
78	10173.0
79	5290.0
80	5290.0
81	5290.0
82	3599.0
83	1908.0
84	1908.0
85	1908.0
86	1270.0
87	632.0
88	632.0
89	632.0
90	414.0
91	196.0
92	196.0
93	196.0
94	127.5
95	59.0
96	59.0
97	59.0
98	38.0
99	17.0
100	17.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0296071625564984
2	0.016586211484530516
3	0.02309668702051446
4	0.02803121411326419
5	0.03012386696411617
6	0.01493275738015364
7	0.00519287929655862
8	0.00480535099084529
9	0.0058645950264617245
10	0.002841874241897752
11	0.0021443232916137584
12	0.0066913220786501615
13	0.00268686291961242
14	0.0012400905782826554
15	0.0028677094622786407
16	0.0024801811565653108
17	0.0025835220380888654
18	0.0024285107158035337
19	5.425396279986617E-4
20	3.1002264457066385E-4
21	0.08497203983274278
22	0.08300856308379524
23	0.0840419718990308
24	0.0011109144763782122
25	0.23783903882646096
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3870685.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.820367787430925
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.616301436297	50.9203120735967
2	7.3333261903950735	8.333645824868954
3	1.3964998597656118	2.380489069409333
4	0.5025521505054277	1.1422079209633098
5	0.24853759451528554	0.7060998764680949
6	0.16431279895862927	0.5601788201406908
7	0.10616660385051783	0.4222697834270232
8	0.085668328872965	0.3894164763437167
9	0.05774293223542504	0.29528771820673966
>10	0.4230435022007391	4.57786878575693
>50	0.037617102458287865	1.4670419734134985
>100	0.020806926534916878	2.2764296382785516
>500	0.002628575679625363	1.0612706151459497
>1k	0.002951383218626793	3.4341021248008317
>5k	8.300765302387855E-4	3.7728010524906757
>10k+	0.0010145379814029602	18.26057824668907
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	124199	3.20870853608599	No Hit
CCCATGTACTCTGCGTTGATACCAC	118412	3.0592001157417874	No Hit
GAGTACATGGGAAGCAGTGGTATCA	57534	1.4864035693940478	No Hit
CATGTACTCTGCGTTGATACCACTG	46075	1.1903577790494448	No Hit
GCGTTGATACCACTGCTTCCCATGT	38875	1.0043441923070464	No Hit
GTATCAACGCAGAGTACATGGGAAG	33616	0.868476768323953	No Hit
GCTTCCCATGTACTCTGCGTTGATA	32782	0.8469301945262918	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	31496	0.813706101116469	No Hit
ACTCTGCGTTGATACCACTGCTTCC	24575	0.6349005408603386	No Hit
TATCAACGCAGAGTACATGGGAAGC	23529	0.6078769003419291	No Hit
GGTATCAACGCAGAGTACATGGGAA	20095	0.5191587535539575	No Hit
CAGTGGTATCAACGCAGAGTACATG	17993	0.4648531203133296	No Hit
GTGGTATCAACGCAGAGTACATGGG	16459	0.4252218922490464	No Hit
GCAGTGGTATCAACGCAGAGTACAT	14805	0.3824904377390565	No Hit
GTACATGGTAAGCAGTGGTATCAAC	14032	0.3625198123846296	No Hit
ATACCACTGCTTCCCATGTACTCTG	13079	0.3378988473616427	No Hit
GTACTCTGCGTTGATACCACTGCTT	12594	0.32536876547691174	No Hit
CATGGGAAGCAGTGGTATCAACGCA	12376	0.319736687433878	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	11845	0.3060181854116261	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	11536	0.2980351023139315	No Hit
ACATGGGAAGCAGTGGTATCAACGC	10958	0.28310234493377784	No Hit
TACCACTGCTTCCCATGTACTCTGC	10026	0.25902391953878967	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	9954	0.25716378367136566	No Hit
ACCATGTACTCTGCGTTGATACCAC	9905	0.25589785787270214	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	9750	0.2518933987136644	No Hit
CCATGTACTCTGCGTTGATACCACT	9528	0.2461579797891071	No Hit
GATACCACTGCTTCCCATGTACTCT	9443	0.24396198605673153	No Hit
CTTCCCATGTACTCTGCGTTGATAC	9353	0.2416368162224516	No Hit
CCACTGCTTCCCATGTACTCTGCGT	9151	0.23641810170551206	No Hit
GTTGATACCACTGCTTCCCATGTAC	9073	0.23440295451580279	No Hit
GTACATGGGTAAGCAGTGGTATCAA	9027	0.23321453437828188	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	8372	0.2162924650287998	No Hit
ATGGGAAGCAGTGGTATCAACGCAG	8023	0.2072759731158697	No Hit
GGTAAGCAGTGGTATCAACGCAGAG	6951	0.17958061686755702	No Hit
TTCCCATGTACTCTGCGTTGATACC	6720	0.17361268095957175	No Hit
TGATACCACTGCTTCCCATGTACTC	6416	0.1657587739637816	No Hit
CCCCATGTACTCTGCGTTGATACCA	6195	0.1600491902596052	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	5589	0.1443930467087867	No Hit
ATCAACGCAGAGTACATGGGAAGCA	5303	0.13700417367985254	No Hit
GCTTACCATGTACTCTGCGTTGATA	5231	0.13514403781242856	No Hit
GAGTACATGGTAAGCAGTGGTATCA	4826	0.12468077355816864	No Hit
AAAAAGTACTCTGCGTTGATACCAC	4119	0.10641527274888037	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3930	0.10153241609689241	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	2.5835220380888654E-5	0.0
6	0.0	0.0	0.0	2.5835220380888654E-5	0.0
7	0.0	0.0	0.0	2.5835220380888654E-5	0.0
8	0.0	0.0	0.0	2.5835220380888654E-5	0.0
9	2.5835220380888654E-5	0.0	0.0	5.167044076177731E-5	0.0
10	2.5835220380888654E-5	0.0	0.0	1.0334088152355462E-4	0.0
11	2.5835220380888654E-5	0.0	0.0	1.2917610190444327E-4	0.0
12	2.5835220380888654E-5	0.0	0.0	1.5501132228533192E-4	0.0
13	2.5835220380888654E-5	0.0	0.0	1.8084654266622058E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGGTT	55	1.9690045E-4	13.81183	7
CTGATCG	50	0.0015054587	13.293885	9
GTACAAA	935	0.0	12.906651	1
TATCGAC	130	1.9099389E-10	12.454053	19
GTCCTAC	250	0.0	11.402567	1
GTGTACG	60	0.0058684936	11.08583	1
GGTATCG	165	8.0035534E-11	10.945395	17
ACCGTGT	115	8.8799425E-7	10.734194	8
GTATCGA	155	3.6361598E-9	10.425072	18
CGAATTA	75	0.0026430183	10.136139	15
CTAGCAC	235	0.0	10.106438	3
GAACAAA	1630	0.0	10.026798	1
GTCCTAG	270	0.0	9.854071	1
GTCCTGA	385	0.0	9.625544	1
GTACAAG	480	0.0	9.50214	1
GTCTTAG	270	0.0	9.50214	1
CCGGTAC	90	0.0011142476	9.5000515	3
GGGTAAG	2500	0.0	9.45765	7
ACGCAAA	415	0.0	9.387656	1
TGGGTAA	2410	0.0	9.298869	6
>>END_MODULE