FastQCFastQC Report
Wed 25 May 2016
SRR1294702_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294702_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3870685
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC1329293.434250010011148No Hit
GTACATGGGAAGCAGTGGTATCAAC1211963.131125369282181No Hit
GAGTACATGGGAAGCAGTGGTATCA628131.622787697784759No Hit
CATGTACTCTGCGTTGATACCACTG535501.3834760513965876No Hit
GCGTTGATACCACTGCTTCCCATGT425321.0988235932399564No Hit
ACGCAGAGTACATGGGAAGCAGTGG383070.9896697871307018No Hit
GCTTCCCATGTACTCTGCGTTGATA375120.9691307869278952No Hit
GTATCAACGCAGAGTACATGGGAAG350350.9051369460444341No Hit
ACTCTGCGTTGATACCACTGCTTCC291250.7524507935933821No Hit
TATCAACGCAGAGTACATGGGAAGC270780.699566097473703No Hit
GGTATCAACGCAGAGTACATGGGAA220710.5702091490265935No Hit
CAGTGGTATCAACGCAGAGTACATG213480.5515302846912109No Hit
GTGGTATCAACGCAGAGTACATGGG172020.4444174609920466No Hit
GCAGTGGTATCAACGCAGAGTACAT163320.4219408192606735No Hit
CATGGGAAGCAGTGGTATCAACGCA159570.41225261161784027No Hit
ATACCACTGCTTCCCATGTACTCTG152920.3950721900645493No Hit
ACATGGGAAGCAGTGGTATCAACGC138570.3579986488179741No Hit
GTACATGGTAAGCAGTGGTATCAAC136750.35329663870865236No Hit
GGGAAGCAGTGGTATCAACGCAGAG134990.34874963992161595No Hit
CTGCTTCCCATGTACTCTGCGTTGA133420.34469351032181644No Hit
GTACTCTGCGTTGATACCACTGCTT129440.3344110926102227No Hit
AAGCAGTGGTATCAACGCAGAGTAC120400.3110560533858994No Hit
TACCACTGCTTCCCATGTACTCTGC116660.30139368096344704No Hit
CCATGTACTCTGCGTTGATACCACT116210.30023109604630704No Hit
GCAGAGTACATGGGAAGCAGTGGTA114560.2959682846834604No Hit
CTTCCCATGTACTCTGCGTTGATAC113050.29206716640594627No Hit
GATACCACTGCTTCCCATGTACTCT111440.2879076959246231No Hit
ACCATGTACTCTGCGTTGATACCAC109910.2839549072063472No Hit
GTATCAACGCAGAGTACTTTTTTTT109670.28333486191720586No Hit
CCACTGCTTCCCATGTACTCTGCGT106010.2738791712578006No Hit
ATGGGAAGCAGTGGTATCAACGCAG97310.2514025295264275No Hit
GTACATGGGTAAGCAGTGGTATCAA97220.2511700125429995No Hit
GTTGATACCACTGCTTCCCATGTAC96460.24920653579405197No Hit
GGTAAGCAGTGGTATCAACGCAGAG80370.2076376662012021No Hit
TATCAACGCAGAGTACTTTTTTTTT77080.19913787869588975No Hit
GGTATCAACGCAGAGTACTTTTTTT76930.19875035039017644No Hit
TTCCCATGTACTCTGCGTTGATACC70360.18177661059993255No Hit
CCCCATGTACTCTGCGTTGATACCA70020.18089821310698237No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA66180.1709774884807211No Hit
TGATACCACTGCTTCCCATGTACTC66070.17069330105653133No Hit
ATCAACGCAGAGTACATGGGAAGCA62660.1618834909066483No Hit
GCTTACCATGTACTCTGCGTTGATA60100.1552696744891408No Hit
ACGCAGAGTACTTTTTTTTTTTTTT56250.1453231146424987No Hit
GAGTACATGGTAAGCAGTGGTATCA55380.14307545046936138No Hit
GTACTTTTTTTTTTTTTTTTTTTTT54190.14000105924403564No Hit
AAAAAGTACTCTGCGTTGATACCAC46400.11987542256732336No Hit
GGAAGCAGTGGTATCAACGCAGAGT44990.11623265649361805No Hit
GAGTACTTTTTTTTTTTTTTTTTTT40450.10450346644069461No Hit
ACTCTGCGTTGATACCACTGCTTAC39660.1024624840306044No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGC851.6791091E-814.5554873
ATCTACG802.8160375E-511.8966192
CTTAGAC2250.010.1515193
ACGTACT2201.8189894E-129.9496424
TTATACT4150.09.6317134
CCCACGT1804.0272425E-99.5217341
TCGTTCA800.004545279.49307513
TTGCGTG900.00112175819.49307516
CTTACAC4250.09.4050843
CTAGGAC3850.09.3934513
TAGGACC2151.03682396E-109.2957234
ATTATAC4200.09.2904533
GTTAGAC1451.6669728E-69.1888753
ATTAACG1252.6986765E-59.1363673
CACGTAC2201.6189006E-109.08445553
GTCTTAG3150.09.0683191
CGTACTC2106.4574124E-109.0638565
TTAACGC1054.415445E-49.0638564
GTATTAT4850.09.0309241
GTACGGA950.00178718129.0205911
Produced by FastQC (version 0.11.5)