FastQCFastQC Report
Wed 25 May 2016
SRR1294701_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294701_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4766030
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC709031.4876742278164425No Hit
CCCATGTACTCTGCGTTGATACCAC681471.4298483223983063No Hit
GAGTACATGGGAAGCAGTGGTATCA333370.6994710482309175No Hit
CATGTACTCTGCGTTGATACCACTG269650.5657748692307853No Hit
GCGTTGATACCACTGCTTCCCATGT233150.4891912136516136No Hit
GTATCAACGCAGAGTACATGGGAAG193710.4064389019792154No Hit
ACGCAGAGTACATGGGAAGCAGTGG185270.3887302429904973No Hit
GCTTCCCATGTACTCTGCGTTGATA170550.35784499887747245No Hit
ACTCTGCGTTGATACCACTGCTTCC150890.3165947339819514No Hit
TATCAACGCAGAGTACATGGGAAGC141330.29653611076724234No Hit
GGTATCAACGCAGAGTACATGGGAA122270.25654475527850223No Hit
GTGGTATCAACGCAGAGTACATGGG104880.22005736430530232No Hit
CAGTGGTATCAACGCAGAGTACATG99800.20939859799455732No Hit
GCAGTGGTATCAACGCAGAGTACAT80500.16890367874310483No Hit
ATACCACTGCTTCCCATGTACTCTG80190.16825324221626806No Hit
GTACTCTGCGTTGATACCACTGCTT72900.15295749292388003No Hit
ACATGGGAAGCAGTGGTATCAACGC70030.14693570959477806No Hit
GTACATGGTAAGCAGTGGTATCAAC69290.14538305465974827No Hit
CATGGGAAGCAGTGGTATCAACGCA69080.14494243636737494No Hit
CTGCTTCCCATGTACTCTGCGTTGA67040.14066214438431984No Hit
GGGAAGCAGTGGTATCAACGCAGAG64260.13482919746623498No Hit
TACCACTGCTTCCCATGTACTCTGC59700.12526148597470013No Hit
CTTCCCATGTACTCTGCGTTGATAC58360.12244992163288942No Hit
GATACCACTGCTTCCCATGTACTCT57270.12016290287723745No Hit
GTTGATACCACTGCTTCCCATGTAC55760.11699464753683883No Hit
GCAGAGTACATGGGAAGCAGTGGTA55170.11575671995350427No Hit
AAGCAGTGGTATCAACGCAGAGTAC54760.11489646519220399No Hit
CCATGTACTCTGCGTTGATACCACT53810.11290319196480089No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA53570.11239962820208853No Hit
CCACTGCTTCCCATGTACTCTGCGT51810.10870682727553121No Hit
ACCATGTACTCTGCGTTGATACCAC49450.10375511694219298No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGTT400.005266685514.25592215
AACCGCC750.002658627210.1290367
GGTATCG856.5623387E-410.06479917
TACACCG951.6500519E-49.9972285
TAGGACC3000.09.8150784
GTAGGAC3050.09.65508653
ACTATCG1106.873694E-59.49607111
CTATCGG1201.7105342E-59.49607112
GTTCTAG4050.09.3830691
CGCAAAA2750.09.3267422
TAGGACT4600.09.2919754
TCTAGAC4100.09.2676693
TAGACAG5750.09.2496095
GTCCTAG4250.09.16505051
TATACTG9900.09.1136345
ACCGCCC1151.11671696E-49.0831038
ACGCAAA3350.09.0749681
CGGCGTT950.00183195438.9961839
CTAATAC5800.08.8442323
TTAGGAC2502.5465852E-118.7394113
Produced by FastQC (version 0.11.5)