##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294701_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4766030
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.257811637778193	33.0	33.0	33.0	27.0	33.0
2	31.233469575306913	33.0	33.0	33.0	27.0	33.0
3	31.25840940992818	33.0	33.0	33.0	27.0	33.0
4	34.66389867457821	37.0	37.0	37.0	27.0	37.0
5	34.93014563483654	37.0	37.0	37.0	33.0	37.0
6	35.08224769881851	37.0	37.0	37.0	33.0	37.0
7	35.12249755037211	37.0	37.0	37.0	33.0	37.0
8	35.05577954817741	37.0	37.0	37.0	33.0	37.0
9	34.75936890871438	37.0	37.0	37.0	27.0	37.0
10	34.86291693505916	37.0	37.0	37.0	33.0	37.0
11	35.089914247287574	37.0	37.0	37.0	33.0	37.0
12	35.024782261127186	37.0	37.0	37.0	33.0	37.0
13	34.92782987098277	37.0	37.0	37.0	33.0	37.0
14	36.18192227073686	40.0	37.0	40.0	27.0	40.0
15	36.45576171362748	40.0	37.0	40.0	33.0	40.0
16	36.46062613957528	40.0	37.0	40.0	33.0	40.0
17	36.42637520116323	40.0	37.0	40.0	27.0	40.0
18	36.42260476748992	40.0	37.0	40.0	27.0	40.0
19	36.47689439638441	40.0	37.0	40.0	33.0	40.0
20	36.387774311114285	40.0	37.0	40.0	27.0	40.0
21	36.32655375648076	40.0	37.0	40.0	27.0	40.0
22	36.20970975843627	40.0	37.0	40.0	27.0	40.0
23	35.8328789369769	40.0	37.0	40.0	27.0	40.0
24	36.15915804138874	40.0	37.0	40.0	27.0	40.0
25	35.085050031158005	37.0	33.0	40.0	27.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.3991513163113467
1101	2	0.5624789254873441
1101	3	0.37889451182964606
1101	4	0.4679191185906859
1101	5	0.464265772445259
1101	6	0.3261654977281836
1101	7	0.4080310800977145
1101	8	0.5069837664707038
1101	9	0.6153355944081085
1101	10	0.7222398604678375
1101	11	0.4795553560400023
1101	12	0.49417400398377254
1101	13	0.46718329529029035
1101	14	1.090793129441849
1101	15	0.9226993124078788
1101	16	0.8891950677692293
1101	17	0.8867442359578988
1101	18	0.840894335251086
1101	19	0.7943481368147189
1101	20	0.8233434687511192
1101	21	0.8459103013895728
1101	22	0.9081686274651517
1101	23	1.2394223772254236
1101	24	1.0644191886395333
1101	25	0.7939508844126308
1102	1	0.2180777858547387
1102	2	0.4195663486935217
1102	3	0.3077402521208654
1102	4	0.2046936793906795
1102	5	0.23731135102249823
1102	6	0.17260019417241068
1102	7	0.28594147526238345
1102	8	0.3288286044447517
1102	9	0.5137109906564632
1102	10	0.604158013427643
1102	11	0.35913584559272493
1102	12	0.3806774948543392
1102	13	0.3914042351507021
1102	14	0.9964509969941062
1102	15	0.7138807377016292
1102	16	0.5300183796670339
1102	17	0.7373549495992577
1102	18	0.7479934187979325
1102	19	0.7131857259524566
1102	20	0.7976663534549999
1102	21	0.8059840281030688
1102	22	0.8975720645894185
1102	23	1.1974156120108432
1102	24	0.9585386291669948
1102	25	0.7537870125503261
1103	1	0.36762064141574413
1103	2	0.5779465044600229
1103	3	0.3420657874160149
1103	4	0.19251016140056265
1103	5	0.3075899794497303
1103	6	0.3118389368592105
1103	7	0.36450331906753775
1103	8	0.5415546139620773
1103	9	0.7831590850363384
1103	10	0.6916239906294308
1103	11	0.5628871004061793
1103	12	0.5203101808014097
1103	13	0.5496151953361448
1103	14	1.2014483338652298
1103	15	0.873308765473098
1103	16	0.5968934690363952
1103	17	0.6413752230970715
1103	18	0.722776929003679
1103	19	0.7617387592656044
1103	20	0.8998998641706848
1103	21	1.0021539721492658
1103	22	0.8560804543299483
1103	23	1.2690454114966983
1103	24	0.9485737400078946
1103	25	0.8449562270719682
1104	1	0.2786440686864218
1104	2	0.5293174935283353
1104	3	0.3474729085063153
1104	4	0.22942763642719655
1104	5	0.21028670766430224
1104	6	0.1464970669718184
1104	7	0.15963117842947838
1104	8	0.4002798527257383
1104	9	0.5158990831831289
1104	10	0.5315786852660409
1104	11	0.3153561053306362
1104	12	0.3051131493919712
1104	13	0.34970120280058836
1104	14	0.8949003929384745
1104	15	0.6702734659273801
1104	16	0.5757024209711545
1104	17	0.6374661801692056
1104	18	0.533974197506403
1104	19	0.5641622309464367
1104	20	0.7070241912666333
1104	21	0.6999098695288026
1104	22	0.7897565021479451
1104	23	1.1633897175012962
1104	24	0.9698898572270451
1104	25	0.7610919783056715
1105	1	0.2960115118127824
1105	2	0.3509485252574187
1105	3	0.3940157268341018
1105	4	0.36233978719192805
1105	5	0.28933263969934586
1105	6	0.323606401843449
1105	7	0.30251753622626865
1105	8	0.4495428549796969
1105	9	-1.4221259052393762
1105	10	-0.04383925863245963
1105	11	0.1922277875871643
1105	12	0.39580962410056486
1105	13	0.3527125880114781
1105	14	-1.4350125659591555
1105	15	-0.12080128146233449
1105	16	0.13390825832621545
1105	17	0.36619963734131034
1105	18	0.24298401428416838
1105	19	0.4289725078585036
1105	20	0.5904689189070353
1105	21	0.756795246277612
1105	22	0.7031300691310989
1105	23	1.0167900500499343
1105	24	0.8354096918790788
1105	25	0.5114179379943735
1106	1	0.11509659258345906
1106	2	0.20033171732179866
1106	3	0.16245779318547804
1106	4	-1.0399318873259347
1106	5	-0.16983702566272996
1106	6	-0.03213036943653691
1106	7	0.009558133820092962
1106	8	0.18327034863889935
1106	9	0.4032117630333687
1106	10	0.510130032242067
1106	11	0.35580539783774867
1106	12	0.22694598453360015
1106	13	0.259403889250585
1106	14	0.5941576751333315
1106	15	0.4023507851828825
1106	16	0.2829600231788092
1106	17	0.19582667631682682
1106	18	0.37988506056071003
1106	19	0.31179028775127904
1106	20	0.4460757925069814
1106	21	0.5944392117270993
1106	22	0.5340121287266015
1106	23	0.8045186072575845
1106	24	0.5180767068506995
1106	25	0.42491133871443765
1107	1	0.4286949529961319
1107	2	0.3355261580385793
1107	3	0.4271435311695839
1107	4	0.5564011558600654
1107	5	0.5395688242560723
1107	6	0.37360416535518226
1107	7	0.38377976746662057
1107	8	0.427922637467681
1107	9	0.6059583331594496
1107	10	0.6326016452278864
1107	11	0.38120926519079745
1107	12	0.39088023302652175
1107	13	0.3199387756081151
1107	14	0.936024326980295
1107	15	0.6454665353006135
1107	16	0.667154511068766
1107	17	0.6500453259190095
1107	18	0.6824288865579007
1107	19	0.6328962911875209
1107	20	0.8165725403940769
1107	21	0.8237835541448959
1107	22	0.7286623604603335
1107	23	1.0157921774203587
1107	24	0.86782659209495
1107	25	0.8747138037389774
1108	1	0.42666086659430746
1108	2	0.4058570946860165
1108	3	0.4552052712191035
1108	4	0.6374148998040283
1108	5	0.5406560307440174
1108	6	0.33651448339990964
1108	7	0.34506051670077653
1108	8	0.4347127541780367
1108	9	0.21395057250562388
1108	10	0.5257695648835821
1108	11	0.3284313090542952
1108	12	0.3778462326922707
1108	13	0.36249056073609154
1108	14	-1.0569174945283066
1108	15	-0.0207512821441469
1108	16	0.31402943849194287
1108	17	0.4097577554881866
1108	18	0.5480086427362068
1108	19	0.5294230320180233
1108	20	0.6654166847708396
1108	21	0.6818689195154022
1108	22	0.7754955974799529
1108	23	0.9974183445125817
1108	24	0.8729594071102369
1108	25	0.7479588791027467
1109	1	0.28772375358837365
1109	2	0.21823999154931073
1109	3	0.312161218130079
1109	4	0.36336834968454923
1109	5	0.3380986783626341
1109	6	0.18809413358646765
1109	7	0.19952031049970032
1109	8	0.25888616117968155
1109	9	0.43480997998640447
1109	10	0.33008280630699716
1109	11	0.20423980010377107
1109	12	0.12608697751137043
1109	13	0.09550699358143788
1109	14	0.4556683912514359
1109	15	0.24960931011008114
1109	16	0.3256850345008502
1109	17	0.27029083371116513
1109	18	0.31395885897482145
1109	19	0.23901727978805098
1109	20	0.4538599243285546
1109	21	0.43210281245141857
1109	22	0.5192872377815192
1109	23	0.9872060521612767
1109	24	0.6254940559592868
1109	25	0.5928445324398623
1110	1	0.28827444459335894
1110	2	0.189167405985895
1110	3	0.2948458515158734
1110	4	0.47199730818707053
1110	5	0.4475311152190713
1110	6	0.2214688650990908
1110	7	0.23974886653756045
1110	8	0.21751632127229215
1110	9	0.2996562603395887
1110	10	0.4139095850797503
1110	11	0.15405051565746675
1110	12	0.12630303701229195
1110	13	0.08917098637008536
1110	14	0.5252726509563175
1110	15	0.2954057625627087
1110	16	0.3609201902155874
1110	17	0.389265256165757
1110	18	0.3944604072123141
1110	19	0.35308581395242555
1110	20	0.4890749023984
1110	21	0.5022636577541775
1110	22	0.5781223029915807
1110	23	0.6195174444595821
1110	24	0.5823061875755684
1110	25	0.522689355014883
1111	1	0.10903447894650853
1111	2	0.060223748327796756
1111	3	0.24236708253706496
1111	4	0.5117645547493481
1111	5	0.36250827757151427
1111	6	0.28016144757170025
1111	7	0.23094489413748676
1111	8	0.2612524579020743
1111	9	0.2820096272621697
1111	10	0.1634734278967116
1111	11	0.1239303908523155
1111	12	0.1918473899165889
1111	13	0.18269388511961893
1111	14	-1.7806699602646603
1111	15	-0.6128885993461779
1111	16	-0.054447460146867854
1111	17	0.17295950606060018
1111	18	0.2414206701171011
1111	19	0.24958635638758153
1111	20	0.2562661446920771
1111	21	0.37108480400807053
1111	22	0.0926440626561984
1111	23	-2.0136594419833713
1111	24	-0.3928052950337033
1111	25	-0.034319054473129995
1112	1	-0.10742508808702311
1112	2	-0.21941997451082784
1112	3	-0.006015037633861908
1112	4	0.1859937233107729
1112	5	-0.7284893845165286
1112	6	-0.8256416340422561
1112	7	-0.9005296208161155
1112	8	-0.6730383183284587
1112	9	-0.5247551692882837
1112	10	-0.8011087366418934
1112	11	-0.7415876479948622
1112	12	-0.6779000174492467
1112	13	-0.7533824149906962
1112	14	-0.5876941554380082
1112	15	-0.7975895980259082
1112	16	-0.7850539026699082
1112	17	-0.7857593961850711
1112	18	-1.0081121717183592
1112	19	-0.7346050639186146
1112	20	-0.8159103809332819
1112	21	-1.71341494242332
1112	22	-1.6502127626509875
1112	23	-1.2537565348680744
1112	24	-1.5139436084335784
1112	25	-1.8610119964142342
1113	1	0.08748897271513556
1113	2	-0.04356162993002144
1113	3	-0.051345223636609205
1113	4	0.22003742804911752
1113	5	0.13747383288864512
1113	6	0.07433972916815179
1113	7	0.053213702115201045
1113	8	0.06187859269181928
1113	9	0.2264521337400609
1113	10	-0.06835451330372422
1113	11	-0.10233043305407108
1113	12	0.022233470845449688
1113	13	-0.09268828914826344
1113	14	0.01647602517677882
1113	15	-0.03744971529781793
1113	16	0.020112538253194145
1113	17	0.020599497815553036
1113	18	0.06735032706264832
1113	19	-0.1359721767288562
1113	20	-0.6687327735278643
1113	21	-0.5127877064319861
1113	22	-0.0581458308553664
1113	23	-0.2794182061328314
1113	24	0.061131802979751626
1113	25	-1.0614274676497288
1114	1	-0.9744904725752903
1114	2	-0.7376906744100502
1114	3	-0.5573357165507851
1114	4	-1.5637619379428642
1114	5	-0.79317824622985
1114	6	-0.6440208128693499
1114	7	-0.49334991971691977
1114	8	-0.4512567047987375
1114	9	-0.5409110653279896
1114	10	-0.623909674604306
1114	11	-0.6020744661348374
1114	12	-0.6367960185184884
1114	13	-0.6473564409823354
1114	14	-0.6844450495249532
1114	15	-1.357864103462866
1114	16	-0.858487373354123
1114	17	-0.8561544816813083
1114	18	-0.8419620986690148
1114	19	-0.825468416652285
1114	20	-2.117399472873096
1114	21	-6.8014634650072345
1114	22	-7.913803624574815
1114	23	-8.053635056939676
1114	24	-6.000686347427777
1114	25	-7.531383983698884
1115	1	-1.0315277532574534
1115	2	-1.0317686143241573
1115	3	-0.8631299458205497
1115	4	-0.576785829338796
1115	5	-0.7178215211248684
1115	6	-0.9175702187503063
1115	7	-1.0855206838519322
1115	8	-0.8334489114958217
1115	9	-2.783149888143548
1115	10	-1.5846496311028133
1115	11	-1.1464560616119428
1115	12	-1.0371950021339984
1115	13	-1.4434193567593212
1115	14	-1.0928191318679694
1115	15	-2.0557271666272356
1115	16	-1.417543546539143
1115	17	-3.2938702279445167
1115	18	-2.8459821303054227
1115	19	-2.1671358121141537
1115	20	-1.7777307002546578
1115	21	-1.292175936568853
1115	22	-0.960970802381695
1115	23	-0.2754316536532073
1115	24	-1.3687970340299103
1115	25	-1.0804152406939878
1116	1	-1.6228010335768595
1116	2	-0.91240042592311
1116	3	-0.7427003694836749
1116	4	-0.4357332301269139
1116	5	-0.40003690804835657
1116	6	-0.4419934946432562
1116	7	-0.8933708288439135
1116	8	-1.8307440896254477
1116	9	-0.9959545960071736
1116	10	-2.572226578898267
1116	11	-1.1556081778325975
1116	12	-0.9201154805703666
1116	13	-0.9676838544369488
1116	14	-1.2920376078539064
1116	15	-1.4133147818588654
1116	16	-0.9813067017484798
1116	17	-0.8645403537844416
1116	18	-1.4614499127559526
1116	19	-1.0130420947846588
1116	20	-1.281938559489106
1116	21	-0.7895532576738873
1116	22	-0.3789415127173825
1116	23	-0.12376478650045897
1116	24	-0.2886761409252543
1116	25	-0.21649800404990316
1201	1	0.3310472921922809
1201	2	0.46141011872852644
1201	3	0.32993244705456704
1201	4	0.3571860596060077
1201	5	0.3636504931664959
1201	6	0.25640226443004366
1201	7	0.3669370372176175
1201	8	0.47253587607425374
1201	9	0.6983963127414938
1201	10	0.6535312096290582
1201	11	0.47907851825424075
1201	12	0.5312865589227727
1201	13	0.4974630273136853
1201	14	1.1594229931183335
1201	15	0.8406513391303463
1201	16	0.8053813179473011
1201	17	0.7291184240135848
1201	18	0.6091135323322021
1201	19	0.6796894844929469
1201	20	0.8200877125050638
1201	21	0.8948370456207968
1201	22	0.9495929414507884
1201	23	0.7062846471562594
1201	24	0.7697426692608573
1201	25	0.79433049148777
1202	1	-0.16367631637635327
1202	2	0.09960764686691803
1202	3	0.06622401204515072
1202	4	-0.01568731062455697
1202	5	-0.022554073895236115
1202	6	-0.11544492568514642
1202	7	-7.926628537262559E-4
1202	8	0.0752305874899335
1202	9	0.2331518205507095
1202	10	0.39995803333138014
1202	11	0.21431927019315822
1202	12	0.1413748585251895
1202	13	0.28897718672091344
1202	14	0.4543018139009547
1202	15	0.4743327191020441
1202	16	0.41779046854762925
1202	17	0.21198205640779122
1202	18	0.2580423703331647
1202	19	0.37552808356265643
1202	20	0.47532315888847165
1202	21	0.49090236656571307
1202	22	0.3886002258796424
1202	23	0.6969176229962031
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2211	5	0.35412757455781474
2211	6	0.2979061194076067
2211	7	0.27652747010717604
2211	8	0.3015174063052015
2211	9	0.48442803273736246
2211	10	0.44980507303493056
2211	11	0.234726724915312
2211	12	0.2451198103651464
2211	13	0.20296642975517898
2211	14	0.5223808289121834
2211	15	0.375913198614235
2211	16	0.3381105131873241
2211	17	0.3588232796963169
2211	18	0.3822476814508917
2211	19	0.31923348483856984
2211	20	0.40799006137229554
2211	21	0.4583917823480519
2211	22	0.5134956842733729
2211	23	0.6530051885592769
2211	24	0.5655086744291395
2211	25	0.7100149669376847
2212	1	-0.20299365180642326
2212	2	-0.30003056342131984
2212	3	-0.16032217430132079
2212	4	-0.4079157672143481
2212	5	-0.07323948884373976
2212	6	-0.4229367548018317
2212	7	-0.4659130473994537
2212	8	-0.2583951684183319
2212	9	-0.1866129388565554
2212	10	-0.4455204275187228
2212	11	-0.32729659307101855
2212	12	-0.5777181981956261
2212	13	-0.5246506616943947
2212	14	-0.3336375184545517
2212	15	-0.5264581930764649
2212	16	-0.33676469734894
2212	17	-0.3072061935310799
2212	18	-0.3324266365859785
2212	19	-0.40614589432889403
2212	20	-0.4031599637344243
2212	21	-0.3231353437401623
2212	22	-0.4271474352087239
2212	23	0.09710236204115574
2212	24	-0.1493934269794579
2212	25	-0.9223026377245702
2213	1	-0.10282825551767161
2213	2	-1.3164214147735152
2213	3	-0.283110166675268
2213	4	0.1374416790421833
2213	5	0.05884216125176067
2213	6	-0.028611547230688927
2213	7	-0.01275653131211385
2213	8	0.12483121530037522
2213	9	0.1739766385278898
2213	10	-0.35585900304619855
2213	11	-0.12868369229812515
2213	12	-0.0681007497989583
2213	13	-0.37700242350722135
2213	14	-0.019011146503174814
2213	15	-0.11992906533458836
2213	16	-0.07697966779471699
2213	17	0.11674007177117574
2213	18	0.10239774641222965
2213	19	0.08072646770008163
2213	20	0.19001233846011445
2213	21	-0.5580968057511484
2213	22	-0.8295621797356247
2213	23	-0.15358157070892275
2213	24	0.06324332086224871
2213	25	-0.5756258919885937
2214	1	-0.2168324348921722
2214	2	-0.5040612421611463
2214	3	-0.43160085885482147
2214	4	-0.5142563926621406
2214	5	-0.17379347687521118
2214	6	-0.17718911404877957
2214	7	-0.5102052221372588
2214	8	-0.13211992976420817
2214	9	-0.087614637923771
2214	10	-1.952097318839833
2214	11	-0.8787192206887013
2214	12	-0.5516735959752381
2214	13	-0.25151460325739805
2214	14	-0.6339700818625147
2214	15	-0.4741405698599763
2214	16	-0.7390589498617217
2214	17	-0.4951563781084616
2214	18	-0.3999824703912793
2214	19	-1.335154908255788
2214	20	-2.2707102533946752
2214	21	-1.8340687389404735
2214	22	-2.0370414311555223
2214	23	-2.488699253096236
2214	24	-1.6677981120379002
2214	25	-2.0775375047674416
2215	1	-0.2307967988858266
2215	2	-0.3167586951125472
2215	3	-0.8575931622836386
2215	4	-0.2770892599183341
2215	5	-0.24808459873548827
2215	6	-0.22465812621717163
2215	7	-0.2560461134782983
2215	8	-0.33946095966872747
2215	9	-0.2111679092814427
2215	10	-0.2698762723076982
2215	11	-0.6411001820672837
2215	12	-0.49352952775161896
2215	13	-0.3169124693798864
2215	14	-0.13567787668780085
2215	15	-0.6787144241919734
2215	16	-0.4831252839760438
2215	17	-0.32957271096312013
2215	18	-0.3782651650729605
2215	19	-2.228701629340108
2215	20	-2.2907670014358956
2215	21	-3.2005124443797186
2215	22	-2.4209674382600497
2215	23	-3.4664349369699607
2215	24	-2.471893922382293
2215	25	-1.725027616374021
2216	1	-1.4362309080472997
2216	2	-1.1520540046839507
2216	3	-0.7647979209040585
2216	4	-0.8521185824033211
2216	5	-1.727426177434701
2216	6	-1.3605839179242665
2216	7	-1.0967657905218147
2216	8	-5.009719192370408
2216	9	-3.873887438251277
2216	10	-1.8654628973578653
2216	11	-1.060899776033672
2216	12	-1.2362326127498093
2216	13	-1.8371744039235267
2216	14	-3.3079475734095283
2216	15	-1.9258529003579596
2216	16	-2.8587003358992504
2216	17	-2.4993799034472133
2216	18	-2.5374979485853473
2216	19	-1.5525336982021614
2216	20	-0.951415642080562
2216	21	-0.7681563973234589
2216	22	-0.6417636070484605
2216	23	-1.0073106758771004
2216	24	-0.5889888337134366
2216	25	-0.3331656288355944
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	46.0
8	265.0
9	808.0
10	1655.0
11	3323.0
12	5910.0
13	8844.0
14	12436.0
15	16859.0
16	20137.0
17	22101.0
18	22910.0
19	24057.0
20	24824.0
21	26742.0
22	28526.0
23	30454.0
24	32817.0
25	35614.0
26	39125.0
27	43541.0
28	49879.0
29	59368.0
30	71663.0
31	88336.0
32	119979.0
33	172833.0
34	253946.0
35	406133.0
36	720507.0
37	2422392.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.67572337039255	19.92395063780594	12.370792796865675	24.029533194935834
2	14.500810650984427	21.321799691352457	38.924656674723515	25.25273298293961
3	19.79944920092582	26.22163293478783	28.435133367204767	25.543784497081578
4	12.85899202489445	17.527540997230265	36.056513660426965	33.55695331744832
5	12.896762939888207	38.439658624171805	35.04856471559989	13.6150137203401
6	32.487783628803605	35.84185108029484	17.39221392036823	14.278151370533324
7	26.256203278705037	32.72072677709825	22.597223516101877	18.425846428094843
8	25.229242782810047	36.89806393892427	20.73739980365156	17.135293474614127
9	25.09372089417297	17.57983035295959	21.05951032865416	36.26693842421328
10	15.269780202718069	30.846377738443838	32.829244813500885	21.05459724533721
11	31.674154787889954	26.224167765248502	21.519880536962603	20.581796909898944
12	22.028623278187638	28.44551835183565	30.103267302156684	19.422591067820036
13	27.616214415144825	22.98885086904303	25.044503362616165	24.350431353195976
14	20.830812879628585	24.185298091253326	26.994253260643553	27.98963576847453
15	25.805531781589863	28.660449929090753	22.76565579461476	22.768362494704625
16	21.17101767961829	30.1878747948988	27.259302467089384	21.381805058393518
17	22.410043435302047	29.884205400027152	27.570285729632708	20.135465435038093
18	22.725048615721363	28.38365001044913	28.46380195503602	20.42749941879349
19	21.97609533331305	29.55903411969958	28.761386609148314	19.703483937839053
20	22.869481417478895	28.196116207033512	27.74238246617559	21.192019909312002
21	21.456547034191317	28.90343395721639	28.457135225386843	21.18288378320545
22	21.6760244375783	28.616237037957877	26.450398494469123	23.257340029994705
23	20.59989340939988	29.08823039024843	27.897611706990922	22.414264493360765
24	21.49863344007594	30.466040366917042	26.832728281390906	21.202597911616117
25	22.283707322325174	27.529527743261774	25.714428708324334	24.472336226088714
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	7320.0
1	7320.0
2	8549.5
3	9779.0
4	9779.0
5	9779.0
6	11964.5
7	14150.0
8	14150.0
9	14150.0
10	17436.0
11	20722.0
12	20722.0
13	20722.0
14	30308.5
15	39895.0
16	39895.0
17	39895.0
18	59753.5
19	79612.0
20	79612.0
21	79612.0
22	111854.0
23	144096.0
24	144096.0
25	144096.0
26	187102.0
27	230108.0
28	230108.0
29	230108.0
30	276552.0
31	322996.0
32	322996.0
33	322996.0
34	364051.5
35	405107.0
36	405107.0
37	405107.0
38	436439.0
39	467771.0
40	467771.0
41	467771.0
42	494930.5
43	522090.0
44	522090.0
45	522090.0
46	650189.5
47	778289.0
48	778289.0
49	778289.0
50	703423.5
51	628558.0
52	628558.0
53	628558.0
54	519601.5
55	410645.0
56	410645.0
57	410645.0
58	348674.0
59	286703.0
60	286703.0
61	286703.0
62	242702.0
63	198701.0
64	198701.0
65	198701.0
66	155897.0
67	113093.0
68	113093.0
69	113093.0
70	83196.5
71	53300.0
72	53300.0
73	53300.0
74	37782.0
75	22264.0
76	22264.0
77	22264.0
78	14833.5
79	7403.0
80	7403.0
81	7403.0
82	4933.0
83	2463.0
84	2463.0
85	2463.0
86	1589.5
87	716.0
88	716.0
89	716.0
90	453.0
91	190.0
92	190.0
93	190.0
94	113.5
95	37.0
96	37.0
97	37.0
98	29.5
99	22.0
100	22.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.031325862405398204
2	0.015190840175156262
3	0.01999567774437005
4	0.019974695920923706
5	0.017666695341825377
6	0.006168656093226438
7	4.196364689269686E-5
8	2.727637048025296E-4
9	3.566909985879233E-4
10	4.196364689269686E-5
11	7.763274675148919E-4
12	0.0010281093488710728
13	0.0020562186977421456
14	4.825819392660138E-4
15	0.0016575640522615259
16	0.0018883641101713586
17	0.001909345933617707
18	0.0017205095226005712
19	3.1472735169522643E-4
20	3.9865464548062015E-4
21	0.0833607845523423
22	0.08090591120911954
23	0.0814934022656173
24	0.0010071275254247246
25	0.2221345648264908
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4766030.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.66352313065103
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.95434054443578	56.440648285194136
2	9.861839464651926	12.951262475958778
3	2.1082946696680906	4.153141674239367
4	0.7278790176921592	1.9118040285817859
5	0.36184765264485336	1.1880095854608552
6	0.20333498415431484	0.8011014861172446
7	0.14172964037378707	0.6514527263288115
8	0.09634637331684436	0.5061153850275959
9	0.07309038446516652	0.4319434935860974
>10	0.41449094715050483	5.049871964188534
>50	0.03398393587177048	1.54092569692968
>100	0.019078085232661933	2.2549204889698222
>500	0.001226581392129723	0.5463267341678127
>1k	0.001549365507723458	2.1006317288610217
>5k	5.810120653960447E-4	2.4868288525483084
>10k+	3.8734137693069643E-4	6.9850153938401345
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	70903	1.4876742278164425	No Hit
CCCATGTACTCTGCGTTGATACCAC	68147	1.4298483223983063	No Hit
GAGTACATGGGAAGCAGTGGTATCA	33337	0.6994710482309175	No Hit
CATGTACTCTGCGTTGATACCACTG	26965	0.5657748692307853	No Hit
GCGTTGATACCACTGCTTCCCATGT	23315	0.4891912136516136	No Hit
GTATCAACGCAGAGTACATGGGAAG	19371	0.4064389019792154	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	18527	0.3887302429904973	No Hit
GCTTCCCATGTACTCTGCGTTGATA	17055	0.35784499887747245	No Hit
ACTCTGCGTTGATACCACTGCTTCC	15089	0.3165947339819514	No Hit
TATCAACGCAGAGTACATGGGAAGC	14133	0.29653611076724234	No Hit
GGTATCAACGCAGAGTACATGGGAA	12227	0.25654475527850223	No Hit
GTGGTATCAACGCAGAGTACATGGG	10488	0.22005736430530232	No Hit
CAGTGGTATCAACGCAGAGTACATG	9980	0.20939859799455732	No Hit
GCAGTGGTATCAACGCAGAGTACAT	8050	0.16890367874310483	No Hit
ATACCACTGCTTCCCATGTACTCTG	8019	0.16825324221626806	No Hit
GTACTCTGCGTTGATACCACTGCTT	7290	0.15295749292388003	No Hit
ACATGGGAAGCAGTGGTATCAACGC	7003	0.14693570959477806	No Hit
GTACATGGTAAGCAGTGGTATCAAC	6929	0.14538305465974827	No Hit
CATGGGAAGCAGTGGTATCAACGCA	6908	0.14494243636737494	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	6704	0.14066214438431984	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	6426	0.13482919746623498	No Hit
TACCACTGCTTCCCATGTACTCTGC	5970	0.12526148597470013	No Hit
CTTCCCATGTACTCTGCGTTGATAC	5836	0.12244992163288942	No Hit
GATACCACTGCTTCCCATGTACTCT	5727	0.12016290287723745	No Hit
GTTGATACCACTGCTTCCCATGTAC	5576	0.11699464753683883	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	5517	0.11575671995350427	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	5476	0.11489646519220399	No Hit
CCATGTACTCTGCGTTGATACCACT	5381	0.11290319196480089	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	5357	0.11239962820208853	No Hit
CCACTGCTTCCCATGTACTCTGCGT	5181	0.10870682727553121	No Hit
ACCATGTACTCTGCGTTGATACCAC	4945	0.10375511694219298	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	2.098182344634843E-5	0.0
12	0.0	2.098182344634843E-5	0.0	6.294547033904529E-5	0.0
13	0.0	2.098182344634843E-5	0.0	8.392729378539372E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCGTT	40	0.0052666855	14.255922	15
AACCGCC	75	0.0026586272	10.129036	7
GGTATCG	85	6.5623387E-4	10.064799	17
TACACCG	95	1.6500519E-4	9.997228	5
TAGGACC	300	0.0	9.815078	4
GTAGGAC	305	0.0	9.6550865	3
ACTATCG	110	6.873694E-5	9.496071	11
CTATCGG	120	1.7105342E-5	9.496071	12
GTTCTAG	405	0.0	9.383069	1
CGCAAAA	275	0.0	9.326742	2
TAGGACT	460	0.0	9.291975	4
TCTAGAC	410	0.0	9.267669	3
TAGACAG	575	0.0	9.249609	5
GTCCTAG	425	0.0	9.1650505	1
TATACTG	990	0.0	9.113634	5
ACCGCCC	115	1.11671696E-4	9.083103	8
ACGCAAA	335	0.0	9.074968	1
CGGCGTT	95	0.0018319543	8.996183	9
CTAATAC	580	0.0	8.844232	3
TTAGGAC	250	2.5465852E-11	8.739411	3
>>END_MODULE