FastQCFastQC Report
Wed 25 May 2016
SRR1294701_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294701_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4766030
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC740811.5543544627289378No Hit
GTACATGGGAAGCAGTGGTATCAAC691431.4507462185508695No Hit
GAGTACATGGGAAGCAGTGGTATCA365400.7666758287295716No Hit
CATGTACTCTGCGTTGATACCACTG298520.6263493935203933No Hit
GCGTTGATACCACTGCTTCCCATGT250870.526371004798543No Hit
ACGCAGAGTACATGGGAAGCAGTGG226600.4754481192942554No Hit
GTATCAACGCAGAGTACATGGGAAG204840.42979167147500125No Hit
GCTTCCCATGTACTCTGCGTTGATA190110.39888544553853No Hit
ACTCTGCGTTGATACCACTGCTTCC175380.3679792196020587No Hit
TATCAACGCAGAGTACATGGGAAGC162350.34063990365146674No Hit
GGTATCAACGCAGAGTACATGGGAA136590.2865907264536732No Hit
GTATCAACGCAGAGTACTTTTTTTT135030.28331756199604285No Hit
CAGTGGTATCAACGCAGAGTACATG120530.25289391799883765No Hit
GTGGTATCAACGCAGAGTACATGGG112320.23566784094938556No Hit
GGTATCAACGCAGAGTACTTTTTTT100910.211727580397102No Hit
TATCAACGCAGAGTACTTTTTTTTT91180.19131226618380495No Hit
CATGGGAAGCAGTGGTATCAACGCA91010.19095557518521705No Hit
ATACCACTGCTTCCCATGTACTCTG90940.19080870242109263No Hit
GCAGTGGTATCAACGCAGAGTACAT90640.19017924771770214No Hit
ACATGGGAAGCAGTGGTATCAACGC86090.1806325180496136No Hit
GGGAAGCAGTGGTATCAACGCAGAG75980.15941989454535535No Hit
CTGCTTCCCATGTACTCTGCGTTGA72580.15228607457359689No Hit
GTACTCTGCGTTGATACCACTGCTT70430.147774982532632No Hit
AAGCAGTGGTATCAACGCAGAGTAC67290.1411866899704786No Hit
GATACCACTGCTTCCCATGTACTCT66660.13986483509335862No Hit
GTACATGGTAAGCAGTGGTATCAAC66350.13921439856652182No Hit
CTTCCCATGTACTCTGCGTTGATAC66260.13902556215550468No Hit
TACCACTGCTTCCCATGTACTCTGC65950.13837512562866788No Hit
CCATGTACTCTGCGTTGATACCACT63310.1328359242388319No Hit
GCAGAGTACATGGGAAGCAGTGGTA61990.1300663235439139No Hit
CCACTGCTTCCCATGTACTCTGCGT60480.1268980682035153No Hit
ACGCAGAGTACTTTTTTTTTTTTTT58760.12328919457074336No Hit
GTTGATACCACTGCTTCCCATGTAC58220.12215617610464055No Hit
ATGGGAAGCAGTGGTATCAACGCAG56290.11810668417949531No Hit
ACCATGTACTCTGCGTTGATACCAC54380.11409915590124275No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGC350.002148232216.3115353
GTCGTAC752.0376372E-411.4226381
TTGAACG752.0452148E-411.4180754
TACCGTA600.00590454511.07679757
GTCCTAT3250.09.6653091
AGTACGT1106.7250905E-59.5150612
TGGACGG2151.03682396E-109.2937815
TATACTG8450.09.1209475
AGCGCAA1252.7770131E-59.11433510
GTATACG1151.0878088E-49.1050011
ATACGAC1151.0925545E-49.1013633
GTCTAGC2451.6370905E-118.9360771
CCTACAC4700.08.7052693
TACGACG1000.00286529358.5635564
TATACCG1000.00286529358.5635565
GGAACGA1451.7043289E-58.51186115
GTCTAGG5600.08.4989861
GTCTTAG4150.08.4866991
GCCTAAG2605.638867E-118.4205341
TGGACAG7350.08.41468055
Produced by FastQC (version 0.11.5)