FastQCFastQC Report
Wed 25 May 2016
SRR1294700_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294700_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4223633
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC1616583.827463228931112No Hit
CCCATGTACTCTGCGTTGATACCAC1586863.7570972667369538No Hit
GAGTACATGGGAAGCAGTGGTATCA757761.7940952729557706No Hit
CATGTACTCTGCGTTGATACCACTG611731.44835027096341No Hit
GCGTTGATACCACTGCTTCCCATGT565851.339723408733666No Hit
GTATCAACGCAGAGTACATGGGAAG508301.2034663049559466No Hit
ACGCAGAGTACATGGGAAGCAGTGG414740.9819508465816039No Hit
GCTTCCCATGTACTCTGCGTTGATA373220.8836468509456196No Hit
ACTCTGCGTTGATACCACTGCTTCC342800.8116235477845731No Hit
TATCAACGCAGAGTACATGGGAAGC311990.7386768689419748No Hit
GGTATCAACGCAGAGTACATGGGAA263740.6244387237243387No Hit
CAGTGGTATCAACGCAGAGTACATG241250.5711907260881804No Hit
GTGGTATCAACGCAGAGTACATGGG230680.5461648774881719No Hit
GCAGTGGTATCAACGCAGAGTACAT197800.4683172046434906No Hit
ATACCACTGCTTCCCATGTACTCTG196540.4653339909030922No Hit
GTACTCTGCGTTGATACCACTGCTT181560.42986689421168933No Hit
GTACATGGTAAGCAGTGGTATCAAC170060.4026391497556724No Hit
CTGCTTCCCATGTACTCTGCGTTGA164130.38859910413617854No Hit
CATGGGAAGCAGTGGTATCAACGCA163530.3871785261645602No Hit
GGGAAGCAGTGGTATCAACGCAGAG161020.3812357749832905No Hit
CCATGTACTCTGCGTTGATACCACT149850.3547893484116636No Hit
TACCACTGCTTCCCATGTACTCTGC148690.35204289766653496No Hit
ACATGGGAAGCAGTGGTATCAACGC145610.3447505974122278No Hit
GATACCACTGCTTCCCATGTACTCT140130.3317759852714476No Hit
GCAGAGTACATGGGAAGCAGTGGTA129810.3073420441596133No Hit
AAGCAGTGGTATCAACGCAGAGTAC123530.2924733280566754No Hit
CCACTGCTTCCCATGTACTCTGCGT122670.2904371662973559No Hit
ACCATGTACTCTGCGTTGATACCAC121960.2887561490309409No Hit
CTTCCCATGTACTCTGCGTTGATAC121580.287856449648916No Hit
GTTGATACCACTGCTTCCCATGTAC120970.28641219537777074No Hit
GTACATGGGTAAGCAGTGGTATCAA109680.25968165321182024No Hit
ATGGGAAGCAGTGGTATCAACGCAG109070.258237398940675No Hit
TTCCCATGTACTCTGCGTTGATACC93720.2218942791667742No Hit
TGATACCACTGCTTCCCATGTACTC92420.21881636022826795No Hit
ATCAACGCAGAGTACATGGGAAGCA82970.19644225717528013No Hit
GGTAAGCAGTGGTATCAACGCAGAG80330.19019171410015975No Hit
CCCCATGTACTCTGCGTTGATACCA79750.1888184887275954No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA75290.17825885913856626No Hit
GAGTACATGGTAAGCAGTGGTATCA59700.14134750817601813No Hit
GCTTACCATGTACTCTGCGTTGATA55570.13156919647137902No Hit
AAAAAGTACTCTGCGTTGATACCAC50170.11878399472681457No Hit
GGAAGCAGTGGTATCAACGCAGAGT47790.11314903543939543No Hit
CTGCGTTGATACCACTGCTTCCCAT47540.11255712795122115No Hit
GCGTTGATACCACTGCTTACCATGT45100.10678011086664016No Hit
GTATCAACGCAGAGTACTTTTTTTT44110.10443615721347002No Hit
TCCATGTACTCTGCGTTGATACCAC42860.1014766197725986No Hit
ACTCTGCGTTGATACCACTGCTTAC42450.1005058914919928No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAAAA4350.012.4459792
TCGACTG550.003076372712.0875335
ACGCAAA4800.011.8750591
GTACAAA7550.011.5762171
GTCTTAG2550.010.8039751
CGCATAG1303.7347309E-710.252819
ATACCGT951.6519555E-49.9960256
CGGACTG1054.1213716E-59.9496025
TATCGAC1356.2640356E-79.87306719
TTTGACG1002.737927E-49.50669116
GTTACAC1502.6598354E-79.4980233
ACCGGAC900.0011164719.4980223
TTACACT5050.09.4035374
AGAACAA10900.09.3239682
CTAGTAC1856.73208E-99.2413193
TGTACGC1752.6859198E-89.2259945
TGATACG1356.792241E-69.15469917
GTCTAAC1151.1114299E-49.0870021
GTCTAGG3150.09.0476641
AAGCGGT2002.6811904E-99.02119810
Produced by FastQC (version 0.11.5)