##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294700_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4223633
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.159036308315613	33.0	33.0	33.0	27.0	33.0
2	31.102626340877627	33.0	33.0	33.0	27.0	33.0
3	31.190883298809343	33.0	33.0	33.0	27.0	33.0
4	34.50927862340312	37.0	37.0	37.0	27.0	37.0
5	34.93711527493037	37.0	37.0	37.0	33.0	37.0
6	35.08116637974938	37.0	37.0	37.0	33.0	37.0
7	35.14039879885397	37.0	37.0	37.0	33.0	37.0
8	35.00837667477264	37.0	37.0	37.0	33.0	37.0
9	34.72620206348421	37.0	37.0	37.0	27.0	37.0
10	34.84165432934159	37.0	37.0	37.0	33.0	37.0
11	35.10554989034322	37.0	37.0	37.0	33.0	37.0
12	35.049286005673316	37.0	37.0	37.0	33.0	37.0
13	34.90352926023639	37.0	37.0	37.0	33.0	37.0
14	36.223670711920285	40.0	37.0	40.0	27.0	40.0
15	36.428334090580314	40.0	37.0	40.0	33.0	40.0
16	36.41993681742708	40.0	37.0	40.0	33.0	40.0
17	36.431303098540994	40.0	37.0	40.0	33.0	40.0
18	36.42785653961885	40.0	37.0	40.0	33.0	40.0
19	36.41166621247632	40.0	37.0	40.0	27.0	40.0
20	36.34809013946051	40.0	37.0	40.0	27.0	40.0
21	36.277881387895206	40.0	37.0	40.0	27.0	40.0
22	36.226137308805	40.0	37.0	40.0	27.0	40.0
23	35.88888523221596	40.0	37.0	40.0	27.0	40.0
24	36.23905486106392	40.0	37.0	40.0	27.0	40.0
25	35.254382423851695	37.0	33.0	40.0	27.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.46920445046112746
1101	2	0.5301049273198686
1101	3	0.41848616745477685
1101	4	0.5088862551711699
1101	5	0.5254791189732657
1101	6	0.3487600581189625
1101	7	0.3858419586959485
1101	8	0.4502721339681912
1101	9	0.6631657725856499
1101	10	0.673947152365443
1101	11	0.4639360879853882
1101	12	0.4639226021068623
1101	13	0.5101479454873115
1101	14	1.1421716863888207
1101	15	0.8765655759010329
1101	16	0.9003772331165649
1101	17	0.9506121923095208
1101	18	0.8750738942336937
1101	19	0.8758216300185424
1101	20	0.9176959873343122
1101	21	0.8723899349441879
1101	22	0.9632765756576518
1101	23	1.299753405176567
1101	24	1.095648023475917
1101	25	0.99793414513816
1102	1	0.37643394068619784
1102	2	0.5508415931352211
1102	3	0.4096995014202207
1102	4	0.44056942162769985
1102	5	0.43144179436355046
1102	6	0.36956861231322335
1102	7	0.41618705096209396
1102	8	0.5319489325847968
1102	9	0.7085699825597302
1102	10	0.7310235571045283
1102	11	0.5706551489358134
1102	12	0.5901300579719546
1102	13	0.6154342220311761
1102	14	1.2438433934565296
1102	15	0.9957448807398706
1102	16	0.7628276154987503
1102	17	0.9480068648741593
1102	18	0.9371683256645582
1102	19	0.8352970888372724
1102	20	0.9522469582236255
1102	21	0.9954122454753502
1102	22	1.106572416569108
1102	23	1.3554442975643184
1102	24	1.170761630941243
1102	25	1.0701019628948032
1103	1	0.3845080194204158
1103	2	0.5065661219688842
1103	3	0.35089852700032864
1103	4	0.2534587695901962
1103	5	0.416907104146091
1103	6	0.32018741880197155
1103	7	0.41209506441761334
1103	8	0.47897413188952953
1103	9	0.6350949554407279
1103	10	0.6330832153140165
1103	11	0.3907035056753969
1103	12	0.46297025514031986
1103	13	0.42390813247740766
1103	14	1.0645319902477226
1103	15	0.8006699905979602
1103	16	0.650041569841477
1103	17	0.6657177087849036
1103	18	0.79907348385467
1103	19	0.8604034225719488
1103	20	0.8928925901097173
1103	21	1.0000334226305867
1103	22	0.9248392275982553
1103	23	1.3676488298965523
1103	24	0.9257421876186029
1103	25	0.9879177745837424
1104	1	0.3395657554410718
1104	2	0.5660591279896394
1104	3	0.3896222339753237
1104	4	0.3865011643727101
1104	5	0.2893481463013785
1104	6	0.27166231262599894
1104	7	0.31683311633575784
1104	8	0.4075834874972344
1104	9	0.6183555580823921
1104	10	0.6384173079697604
1104	11	0.40456863723963465
1104	12	0.3251797276348469
1104	13	0.4599149415454349
1104	14	0.9768936244935063
1104	15	0.8102341541576479
1104	16	0.800242299395002
1104	17	0.7967250016368581
1104	18	0.6650290854858909
1104	19	0.7450653096954554
1104	20	0.7590069012800669
1104	21	0.8632583415382484
1104	22	0.8612481324003696
1104	23	1.2556852874743782
1104	24	0.9578442369315212
1104	25	0.9039923649629245
1105	1	0.2992225915821294
1105	2	0.40774375460000556
1105	3	0.4006399084465784
1105	4	0.38682939582684384
1105	5	0.2929342843512188
1105	6	0.2920792369356704
1105	7	0.2899364623867342
1105	8	0.38479486863101897
1105	9	-1.5374298170529102
1105	10	-0.04838346528013915
1105	11	0.18088360499859846
1105	12	0.19307493099930184
1105	13	0.3221933791925835
1105	14	-1.532994570362625
1105	15	-0.1765042125889238
1105	16	0.06256188775589777
1105	17	0.32211587607045544
1105	18	0.24326764678724544
1105	19	0.38977590813832563
1105	20	0.5405083383977356
1105	21	0.6584823792872072
1105	22	0.628803714795211
1105	23	1.1193358537374323
1105	24	0.8118189213815015
1105	25	0.5008788028851257
1106	1	0.15172306927363977
1106	2	0.14259987308964384
1106	3	0.22124401992045506
1106	4	-1.0787970976867172
1106	5	-0.22287832646661343
1106	6	0.03493498724196087
1106	7	0.22565801870955227
1106	8	0.39720768184621136
1106	9	0.4832968204174435
1106	10	0.5020844809599154
1106	11	0.32982908514699005
1106	12	0.25543596002312796
1106	13	0.21125020531240324
1106	14	0.6731356151513879
1106	15	0.5690726330866269
1106	16	0.3319665239997036
1106	17	0.2830298710579626
1106	18	0.33508973372146045
1106	19	0.29049409850163954
1106	20	0.46077395505543706
1106	21	0.6486865601343581
1106	22	0.6587210955069907
1106	23	0.7852421634925264
1106	24	0.5517948373475292
1106	25	0.5048088083121556
1107	1	0.27332739959618024
1107	2	0.25265693613548734
1107	3	0.20077039195671986
1107	4	0.3637152637633747
1107	5	0.29757015193630565
1107	6	0.14853600962178604
1107	7	0.18360263116576903
1107	8	0.25739069036500695
1107	9	0.4361350684777534
1107	10	0.41982145353303224
1107	11	0.17175627987496256
1107	12	0.23188611806394022
1107	13	0.26040777409249927
1107	14	0.8533483968576405
1107	15	0.6094526402136538
1107	16	0.5204463039048548
1107	17	0.5579800752316473
1107	18	0.6214401699523009
1107	19	0.5303848417541843
1107	20	0.6696035918088015
1107	21	0.6498743979162214
1107	22	0.5906267893371506
1107	23	0.9164931294089982
1107	24	0.7137470916209665
1107	25	0.7261323332964977
1108	1	0.4171492860106518
1108	2	0.39051706874997905
1108	3	0.4246004592550534
1108	4	0.5163699375746305
1108	5	0.5154562425566311
1108	6	0.2262121217618045
1108	7	0.3144617298957897
1108	8	0.3392190951900602
1108	9	0.1527943246971688
1108	10	0.42280437508602375
1108	11	0.2658731060657402
1108	12	0.26577056205704963
1108	13	0.2699712409595705
1108	14	-1.1463088681821247
1108	15	-0.12176116605586884
1108	16	0.2880496232740626
1108	17	0.35404669672852407
1108	18	0.49394151523458873
1108	19	0.4850243567920245
1108	20	0.6295624420311299
1108	21	0.6868071639605802
1108	22	0.6430247143664829
1108	23	0.9643845824158177
1108	24	0.7998267844141225
1108	25	0.6678246511290595
1109	1	0.33830154247829824
1109	2	0.2720567750613334
1109	3	0.3317583531148145
1109	4	0.47562909407910325
1109	5	0.48264016613686067
1109	6	0.25627217745815756
1109	7	0.19292581248915042
1109	8	0.29511747581328507
1109	9	0.46527947993696017
1109	10	0.5000981228804875
1109	11	0.34068149534905245
1109	12	0.38588966899627053
1109	13	0.32824407817529533
1109	14	0.8169256624560859
1109	15	0.4947330495923268
1109	16	0.44380638694511276
1109	17	0.44422423136202127
1109	18	0.5115893369989379
1109	19	0.44119958017533634
1109	20	0.6225994826493206
1109	21	0.6200086501854685
1109	22	0.7837468834512507
1109	23	1.0924659059723467
1109	24	0.8630158582677296
1109	25	0.5879351190359046
1110	1	0.28727826043195037
1110	2	0.15551699046202572
1110	3	0.26231844444879826
1110	4	0.4065370219898554
1110	5	0.36040904275996866
1110	6	0.1642233160657227
1110	7	0.20561463774001965
1110	8	0.2293166276455736
1110	9	0.3549888379861059
1110	10	0.41199940534426815
1110	11	0.15293241628098286
1110	12	0.1304112708571452
1110	13	0.21199425053983845
1110	14	0.5226639219488334
1110	15	0.4197343507099305
1110	16	0.3826180901000029
1110	17	0.3769393266691097
1110	18	0.43939521722779773
1110	19	0.3536570568799675
1110	20	0.48538057994126405
1110	21	0.5215567252736335
1110	22	0.6361279599610796
1110	23	0.7743011149121628
1110	24	0.6880921547308247
1110	25	0.6562109298557317
1111	1	0.05102591320101624
1111	2	0.029800645901321587
1111	3	0.18625549305080114
1111	4	0.31705669647051593
1111	5	0.22277687761798148
1111	6	0.16735880516672097
1111	7	0.021585999246482857
1111	8	0.19974297806243158
1111	9	0.19863222912346146
1111	10	0.15096035528929974
1111	11	0.0975950136982604
1111	12	0.04072793809929465
1111	13	0.0362194646315217
1111	14	-2.013351868381065
1111	15	-0.5931178928314154
1111	16	-0.11301886475877865
1111	17	0.12630561082152525
1111	18	0.11061071480124696
1111	19	0.1601310552043742
1111	20	0.1938093185168981
1111	21	0.3305844618945244
1111	22	0.08006229521480179
1111	23	-2.0641598153634604
1111	24	-0.4126632752726067
1111	25	-0.18207602489139418
1112	1	-0.19265875578852842
1112	2	-0.302657629757757
1112	3	-0.1012004557503694
1112	4	0.08074389119471448
1112	5	-0.7460143893064313
1112	6	-0.8173719356093798
1112	7	-0.8909415451825851
1112	8	-0.6842969643428631
1112	9	-0.5622925676245032
1112	10	-0.8557228976978237
1112	11	-0.7378195647625887
1112	12	-0.6882408462069449
1112	13	-0.8233298011155341
1112	14	-0.5138376248286463
1112	15	-0.7255225712079465
1112	16	-0.7459649850712537
1112	17	-0.7320757512455174
1112	18	-0.9254913805641252
1112	19	-0.7307467668566261
1112	20	-0.909405225737153
1112	21	-1.7344397811805834
1112	22	-1.611154711133345
1112	23	-1.2185234694452092
1112	24	-1.4606353291125203
1112	25	-1.8775455132213068
1113	1	-0.04048382155328056
1113	2	-0.16463337788005106
1113	3	-0.13566083087971137
1113	4	0.04955309233961458
1113	5	-0.06032645913289514
1113	6	-0.15334599392507897
1113	7	-0.15406055106666372
1113	8	-0.04453689628728341
1113	9	0.0448687439125095
1113	10	-0.22903534332286313
1113	11	-0.2410579317376147
1113	12	-0.13160336827867525
1113	13	-0.1964257121181987
1113	14	-0.100936504897426
1113	15	-0.2793278856689838
1113	16	-0.09199149431435671
1113	17	-0.08152933111301053
1113	18	-0.04865357110944757
1113	19	-0.261167950828046
1113	20	-0.8078020651682039
1113	21	-0.6391067940452544
1113	22	-0.15508678682844135
1113	23	-0.4368889837487231
1113	24	-0.08090728115444534
1113	25	-1.185741043020073
1114	1	-1.02946527772934
1114	2	-0.7567084731658547
1114	3	-0.5746597172901211
1114	4	-1.6814147312281307
1114	5	-0.887419083492361
1114	6	-0.7218655237888285
1114	7	-0.5919865994929054
1114	8	-0.4819887121223445
1114	9	-0.4742770977293418
1114	10	-0.5229996579381222
1114	11	-0.5435155241814016
1114	12	-0.6075218365140742
1114	13	-0.609187036201007
1114	14	-0.5365989761751919
1114	15	-1.2990064398286947
1114	16	-0.8246184455671184
1114	17	-0.9067029039282488
1114	18	-0.8940360965072287
1114	19	-0.9204411100556698
1114	20	-2.1146627136607776
1114	21	-6.895333513857135
1114	22	-7.683353300602722
1114	23	-7.722345390748302
1114	24	-5.807632741000425
1114	25	-7.3855820921476045
1115	1	-1.1070782515627755
1115	2	-0.9634127027248915
1115	3	-0.7918000504428129
1115	4	-0.6749698195034028
1115	5	-0.7856028858714694
1115	6	-0.9777521530488897
1115	7	-1.1280013950098038
1115	8	-0.8902438053771391
1115	9	-2.9351931191214256
1115	10	-1.6560369350163384
1115	11	-1.2017322543917786
1115	12	-1.0403170785636533
1115	13	-1.430749303757736
1115	14	-1.0795656257823154
1115	15	-2.05247060579498
1115	16	-1.4226818025386265
1115	17	-3.1574812182371375
1115	18	-2.9968133935426025
1115	19	-2.150173432287062
1115	20	-1.854457954474455
1115	21	-1.464349597551049
1115	22	-1.0192591207539792
1115	23	-0.4877996775678426
1115	24	-1.508633883898213
1115	25	-1.1005309575682887
1116	1	-1.7103303589855194
1116	2	-0.993014650708222
1116	3	-0.8746674505530052
1116	4	-0.49777427220990944
1116	5	-0.4721225324883491
1116	6	-0.6150349328851092
1116	7	-1.0351612884057175
1116	8	-1.9769907867565735
1116	9	-1.0976269587857672
1116	10	-2.794231134908898
1116	11	-1.312666203864545
1116	12	-1.0835643451217436
1116	13	-1.0300871801496143
1116	14	-1.484033161986261
1116	15	-1.476644198878276
1116	16	-1.143880001397548
1116	17	-0.9331259715055822
1116	18	-1.4713059563703013
1116	19	-1.044519742814792
1116	20	-1.380382219187318
1116	21	-0.857581216155566
1116	22	-0.5688044815347695
1116	23	-0.44761498524661647
1116	24	-0.43011039206697177
1116	25	-0.379017415572676
1201	1	0.3346080978351367
1201	2	0.3956653689662346
1201	3	0.31573394471423555
1201	4	0.47554701199062777
1201	5	0.43104818253492283
1201	6	0.36817295327850275
1201	7	0.33823100626072033
1201	8	0.3791016645889229
1201	9	0.6303040726482294
1201	10	0.6401975868236107
1201	11	0.4184455473438149
1201	12	0.44568028686396133
1201	13	0.48139673849268405
1201	14	1.25778506890385
1201	15	0.9432133241594087
1201	16	0.8995470865358399
1201	17	0.8083841595845769
1201	18	0.6873522715602647
1201	19	0.767288329762934
1201	20	0.9099625910690889
1201	21	0.8955600662860022
1201	22	0.9410519200401168
1201	23	0.8636454784433525
1201	24	0.8475278258960728
1201	25	0.7919152924714652
1202	1	0.011275479923803289
1202	2	0.25271834276257366
1202	3	0.14931345358469983
1202	4	0.19482064304236246
1202	5	0.17005926002811833
1202	6	0.1715937981494875
1202	7	0.1902043665798132
1202	8	0.2566339675161018
1202	9	0.30557898009219997
1202	10	0.5072402785713663
1202	11	0.30344980604438376
1202	12	0.26605238389034014
1202	13	0.37504084103818514
1202	14	0.6757532938823232
1202	15	0.6042479055658134
1202	16	0.6064661284621806
1202	17	0.4762502542384297
1202	18	0.5532327589169199
1202	19	0.5806376441622092
1202	20	0.6434452608507755
1202	21	0.7443321883205769
1202	22	0.6517572404251197
1202	23	0.9305598002084139
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2211	6	0.16629611740375339
2211	7	0.1269655869727231
2211	8	0.2903118486981384
2211	9	0.384063168943122
2211	10	0.40220902435086714
2211	11	0.2582808135374606
2211	12	0.2424928704075242
2211	13	0.06556856384438703
2211	14	0.5293435405544074
2211	15	0.33716239651005253
2211	16	0.13097038637653213
2211	17	0.2580524288638415
2211	18	0.174467378045442
2211	19	0.137894714379442
2211	20	0.17960201436343937
2211	21	0.24094939450282027
2211	22	0.3078015736482058
2211	23	0.4050059440062057
2211	24	0.4624720482627467
2211	25	0.4633785421591483
2212	1	-0.2813224546326403
2212	2	-0.26629217461317367
2212	3	-0.2109110609550804
2212	4	-0.5468335621268068
2212	5	-0.22876619417905175
2212	6	-0.5326534133581973
2212	7	-0.4774733920369485
2212	8	-0.3323133697469913
2212	9	-0.21016627896471363
2212	10	-0.5226749524923449
2212	11	-0.42651140121780884
2212	12	-0.5857819613550319
2212	13	-0.6495629345969434
2212	14	-0.46180751758476646
2212	15	-0.6483018129260785
2212	16	-0.5821332094703067
2212	17	-0.4834142937814576
2212	18	-0.46087255425556606
2212	19	-0.49831151010894814
2212	20	-0.5559991037204881
2212	21	-0.4197132455945294
2212	22	-0.5590586730801661
2212	23	0.008366110223818168
2212	24	-0.30552300334103677
2212	25	-0.9558821471071184
2213	1	-0.09336388399365703
2213	2	-1.1677090395351932
2213	3	-0.22447028523595236
2213	4	0.13164527862007702
2213	5	0.07152073538622972
2213	6	0.07453808929722072
2213	7	0.036294420906124
2213	8	0.17083132061419093
2213	9	0.20971500813306676
2213	10	-0.3565677263851228
2213	11	-0.0780755758747631
2213	12	0.06498823065226844
2213	13	-0.3548958834350415
2213	14	-0.029971793171718275
2213	15	-0.11292873239835188
2213	16	-0.08164105557609247
2213	17	0.08831425258626524
2213	18	0.05938121259613638
2213	19	0.04647930045505433
2213	20	0.11839928139954736
2213	21	-0.4420644339251041
2213	22	-0.8003558638618955
2213	23	-0.09445175919199045
2213	24	0.11079209049982808
2213	25	-0.5492423951065462
2214	1	-0.35389669194294626
2214	2	-0.5298513682532473
2214	3	-0.4205297491573319
2214	4	-0.5866642125568973
2214	5	-0.356359168674075
2214	6	-0.34815476350699015
2214	7	-0.6598982450025801
2214	8	-0.26594021519966304
2214	9	-0.20930908581307506
2214	10	-2.095986014110757
2214	11	-0.9353361160027873
2214	12	-0.5162198151994275
2214	13	-0.41338787029005886
2214	14	-0.9080619157440282
2214	15	-0.703056677949462
2214	16	-0.8450474209256669
2214	17	-0.6160464090238378
2214	18	-0.6789598950712517
2214	19	-1.5947135601364693
2214	20	-2.4886156858158657
2214	21	-2.07450579545889
2214	22	-2.175114067796045
2214	23	-2.600628616855488
2214	24	-1.758644731030536
2214	25	-2.117650045362744
2215	1	-0.32397197538485756
2215	2	-0.49437100573288717
2215	3	-0.8738878541025521
2215	4	-0.3911706804173889
2215	5	-0.3302449133127183
2215	6	-0.23579129883666639
2215	7	-0.3421407140889343
2215	8	-0.27752541564353805
2215	9	-0.2313350675079704
2215	10	-0.32727104627876713
2215	11	-0.7125387102791692
2215	12	-0.5294921735750222
2215	13	-0.3188960086070338
2215	14	-0.32142917650995884
2215	15	-0.8306378111814112
2215	16	-0.6350408734899631
2215	17	-0.3186963958339746
2215	18	-0.4010449002366343
2215	19	-2.5146282760685423
2215	20	-2.5965348517306452
2215	21	-3.5074911481701605
2215	22	-2.547870662026952
2215	23	-3.8002040379578332
2215	24	-2.636150065372547
2215	25	-2.059345632660488
2216	1	-1.5074024803713364
2216	2	-1.2477490500085224
2216	3	-0.8472839629339397
2216	4	-0.9232584461784725
2216	5	-1.979454313964922
2216	6	-1.6198745265541845
2216	7	-1.2115525351107976
2216	8	-5.101110707348031
2216	9	-4.121229778394383
2216	10	-1.9976434001154004
2216	11	-1.1362901869543336
2216	12	-1.297589522543909
2216	13	-2.161131613768376
2216	14	-3.632128122328467
2216	15	-2.1731706584711574
2216	16	-3.2725147254485023
2216	17	-2.9662181004904724
2216	18	-2.812497132029371
2216	19	-1.8645955397236946
2216	20	-1.062727386071252
2216	21	-0.9040666443076262
2216	22	-0.793331868365037
2216	23	-1.0409029773049667
2216	24	-0.719486443194775
2216	25	-0.5175193852598525
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	8.0
7	63.0
8	286.0
9	718.0
10	1462.0
11	2710.0
12	4583.0
13	7169.0
14	10838.0
15	16449.0
16	21229.0
17	23244.0
18	23367.0
19	23378.0
20	23436.0
21	25016.0
22	26244.0
23	27013.0
24	29020.0
25	30615.0
26	32618.0
27	36558.0
28	41409.0
29	49259.0
30	59445.0
31	73129.0
32	100183.0
33	146760.0
34	219482.0
35	357188.0
36	643084.0
37	2167670.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.59934879726673	18.97641616345056	11.183369604681385	24.24086543460133
2	12.660082949625211	22.784886260979935	36.95653254938084	27.59849824001402
3	22.041688068145625	28.990591400714074	26.20604290146984	22.761677629670466
4	13.312733822524352	19.590745755021484	33.044998595703184	34.05152182675098
5	12.13902084389105	39.6472499419819	34.54393035933334	13.669798854793717
6	32.73902024767176	32.65382521663997	18.46049406014466	16.1466604755436
7	24.37135173759928	33.74033345124261	22.72476516400992	19.16354964714818
8	26.56907653998986	35.19263146953664	21.647283834602042	16.59100815587145
9	26.918526477338133	18.334105582616505	20.060337253139686	34.68703068690567
10	16.459537770698013	31.261444571928614	31.473774380164116	20.805243277209254
11	28.623128099117785	28.13486943899028	19.511239396142752	23.730763065749183
12	24.16722189153392	30.91361837559909	27.983238601789886	16.93592113107711
13	28.306413099762356	22.64353939255035	22.807596589296104	26.242450918391192
14	21.67647623577723	26.65031725327961	25.338615402937354	26.334591108005807
15	31.13955220081709	24.50680648602063	21.62330459365296	22.73033671950932
16	19.19813975469329	29.42139144776797	30.563209107833757	20.817259689704983
17	25.400843702032116	29.515436025181785	27.201446941645248	17.882273331140855
18	25.775144701096288	26.270819650084505	28.404980284283255	19.549055364535956
19	22.75913259036701	31.046237697135865	29.053771536671235	17.140858175825894
20	23.89494492037978	27.541940987491042	26.86915207430226	21.69396201782692
21	19.644409668913937	29.949591014959193	29.58431366601731	20.821685650109558
22	21.769531664669934	29.173143416084073	22.742015589693818	26.31530932955218
23	18.1417229320659	30.380685364456188	27.221956040716766	24.255635662761144
24	21.020617097051744	34.826268319166054	24.151821842218286	20.001292741563915
25	22.65680858248473	26.41946469340455	22.316187838315965	28.60753888579476
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	9651.0
1	9651.0
2	11710.0
3	13769.0
4	13769.0
5	13769.0
6	17190.5
7	20612.0
8	20612.0
9	20612.0
10	21922.0
11	23232.0
12	23232.0
13	23232.0
14	27764.5
15	32297.0
16	32297.0
17	32297.0
18	44528.0
19	56759.0
20	56759.0
21	56759.0
22	77043.5
23	97328.0
24	97328.0
25	97328.0
26	124700.5
27	152073.0
28	152073.0
29	152073.0
30	182274.5
31	212476.0
32	212476.0
33	212476.0
34	238991.0
35	265506.0
36	265506.0
37	265506.0
38	292778.0
39	320050.0
40	320050.0
41	320050.0
42	375458.0
43	430866.0
44	430866.0
45	430866.0
46	741220.0
47	1051574.0
48	1051574.0
49	1051574.0
50	902943.0
51	754312.0
52	754312.0
53	754312.0
54	545098.0
55	335884.0
56	335884.0
57	335884.0
58	260491.5
59	185099.0
60	185099.0
61	185099.0
62	155723.5
63	126348.0
64	126348.0
65	126348.0
66	100202.5
67	74057.0
68	74057.0
69	74057.0
70	55322.5
71	36588.0
72	36588.0
73	36588.0
74	26501.0
75	16414.0
76	16414.0
77	16414.0
78	11075.0
79	5736.0
80	5736.0
81	5736.0
82	3895.0
83	2054.0
84	2054.0
85	2054.0
86	1363.0
87	672.0
88	672.0
89	672.0
90	436.0
91	200.0
92	200.0
93	200.0
94	125.0
95	50.0
96	50.0
97	50.0
98	38.0
99	26.0
100	26.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.030589778988846808
2	0.013921664121859073
3	0.018798981824415143
4	0.020811467284207696
5	0.019248831515427597
6	0.009044346419303004
7	0.0011127860777676469
8	0.0011838149763485604
9	9.470519810788484E-4
10	3.551444929045682E-4
11	0.001231167575402503
12	0.0028174796437095743
13	0.0025096877498589484
14	8.523467829709636E-4
15	0.0020835143583734666
16	0.002462335150805006
17	0.002462335150805006
18	0.00234395365317015
19	5.445548891203378E-4
20	4.735259905394242E-4
21	0.08409821591980174
22	0.08071250508744486
23	0.08180161486568553
24	0.0012074912758755318
25	0.2263927760768987
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4223633.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.02908998448931
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.9674576844778	40.80909670881071
2	10.191556263723205	9.789823457446952
3	2.368605052802826	3.41285835656349
4	0.874796858726021	1.6806278818360258
5	0.41050239757452983	0.9858028295977851
6	0.23702042150356298	0.6830325091533727
7	0.15661962952099776	0.5265608795720966
8	0.11170666116106763	0.42921354246174204
9	0.08792813279622709	0.3800797382014305
>10	0.5064487607019819	4.640102669657548
>50	0.04726365510840927	1.5737232534720682
>100	0.031044244709508183	2.86299557942757
>500	0.0031500825599934364	1.0014327724800547
>1k	0.0038501009051775696	4.1508788027396335
>5k	4.50011794111664E-4	1.6088245629187794
>10k+	0.0016000419346192498	25.464946455660726
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	161658	3.827463228931112	No Hit
CCCATGTACTCTGCGTTGATACCAC	158686	3.7570972667369538	No Hit
GAGTACATGGGAAGCAGTGGTATCA	75776	1.7940952729557706	No Hit
CATGTACTCTGCGTTGATACCACTG	61173	1.44835027096341	No Hit
GCGTTGATACCACTGCTTCCCATGT	56585	1.339723408733666	No Hit
GTATCAACGCAGAGTACATGGGAAG	50830	1.2034663049559466	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	41474	0.9819508465816039	No Hit
GCTTCCCATGTACTCTGCGTTGATA	37322	0.8836468509456196	No Hit
ACTCTGCGTTGATACCACTGCTTCC	34280	0.8116235477845731	No Hit
TATCAACGCAGAGTACATGGGAAGC	31199	0.7386768689419748	No Hit
GGTATCAACGCAGAGTACATGGGAA	26374	0.6244387237243387	No Hit
CAGTGGTATCAACGCAGAGTACATG	24125	0.5711907260881804	No Hit
GTGGTATCAACGCAGAGTACATGGG	23068	0.5461648774881719	No Hit
GCAGTGGTATCAACGCAGAGTACAT	19780	0.4683172046434906	No Hit
ATACCACTGCTTCCCATGTACTCTG	19654	0.4653339909030922	No Hit
GTACTCTGCGTTGATACCACTGCTT	18156	0.42986689421168933	No Hit
GTACATGGTAAGCAGTGGTATCAAC	17006	0.4026391497556724	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	16413	0.38859910413617854	No Hit
CATGGGAAGCAGTGGTATCAACGCA	16353	0.3871785261645602	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	16102	0.3812357749832905	No Hit
CCATGTACTCTGCGTTGATACCACT	14985	0.3547893484116636	No Hit
TACCACTGCTTCCCATGTACTCTGC	14869	0.35204289766653496	No Hit
ACATGGGAAGCAGTGGTATCAACGC	14561	0.3447505974122278	No Hit
GATACCACTGCTTCCCATGTACTCT	14013	0.3317759852714476	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	12981	0.3073420441596133	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	12353	0.2924733280566754	No Hit
CCACTGCTTCCCATGTACTCTGCGT	12267	0.2904371662973559	No Hit
ACCATGTACTCTGCGTTGATACCAC	12196	0.2887561490309409	No Hit
CTTCCCATGTACTCTGCGTTGATAC	12158	0.287856449648916	No Hit
GTTGATACCACTGCTTCCCATGTAC	12097	0.28641219537777074	No Hit
GTACATGGGTAAGCAGTGGTATCAA	10968	0.25968165321182024	No Hit
ATGGGAAGCAGTGGTATCAACGCAG	10907	0.258237398940675	No Hit
TTCCCATGTACTCTGCGTTGATACC	9372	0.2218942791667742	No Hit
TGATACCACTGCTTCCCATGTACTC	9242	0.21881636022826795	No Hit
ATCAACGCAGAGTACATGGGAAGCA	8297	0.19644225717528013	No Hit
GGTAAGCAGTGGTATCAACGCAGAG	8033	0.19019171410015975	No Hit
CCCCATGTACTCTGCGTTGATACCA	7975	0.1888184887275954	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	7529	0.17825885913856626	No Hit
GAGTACATGGTAAGCAGTGGTATCA	5970	0.14134750817601813	No Hit
GCTTACCATGTACTCTGCGTTGATA	5557	0.13156919647137902	No Hit
AAAAAGTACTCTGCGTTGATACCAC	5017	0.11878399472681457	No Hit
GGAAGCAGTGGTATCAACGCAGAGT	4779	0.11314903543939543	No Hit
CTGCGTTGATACCACTGCTTCCCAT	4754	0.11255712795122115	No Hit
GCGTTGATACCACTGCTTACCATGT	4510	0.10678011086664016	No Hit
GTATCAACGCAGAGTACTTTTTTTT	4411	0.10443615721347002	No Hit
TCCATGTACTCTGCGTTGATACCAC	4286	0.1014766197725986	No Hit
ACTCTGCGTTGATACCACTGCTTAC	4245	0.1005058914919928	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	2.367629952697121E-5	0.0	0.0	0.0	0.0
9	2.367629952697121E-5	0.0	0.0	0.0	0.0
10	2.367629952697121E-5	0.0	0.0	1.4205779716182728E-4	0.0
11	2.367629952697121E-5	2.367629952697121E-5	0.0	1.6573409668879847E-4	0.0
12	2.367629952697121E-5	2.367629952697121E-5	0.0	2.8411559432365457E-4	0.0
13	2.367629952697121E-5	2.367629952697121E-5	0.0	2.8411559432365457E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCAAAA	435	0.0	12.445979	2
TCGACTG	55	0.0030763727	12.087533	5
ACGCAAA	480	0.0	11.875059	1
GTACAAA	755	0.0	11.576217	1
GTCTTAG	255	0.0	10.803975	1
CGCATAG	130	3.7347309E-7	10.2528	19
ATACCGT	95	1.6519555E-4	9.996025	6
CGGACTG	105	4.1213716E-5	9.949602	5
TATCGAC	135	6.2640356E-7	9.873067	19
TTTGACG	100	2.737927E-4	9.506691	16
GTTACAC	150	2.6598354E-7	9.498023	3
ACCGGAC	90	0.001116471	9.498022	3
TTACACT	505	0.0	9.403537	4
AGAACAA	1090	0.0	9.323968	2
CTAGTAC	185	6.73208E-9	9.241319	3
TGTACGC	175	2.6859198E-8	9.225994	5
TGATACG	135	6.792241E-6	9.154699	17
GTCTAAC	115	1.1114299E-4	9.087002	1
GTCTAGG	315	0.0	9.047664	1
AAGCGGT	200	2.6811904E-9	9.021198	10
>>END_MODULE