FastQCFastQC Report
Wed 25 May 2016
SRR1294700_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294700_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4223633
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC1742654.125950337067638No Hit
GTACATGGGAAGCAGTGGTATCAAC1567503.7112599508527375No Hit
GAGTACATGGGAAGCAGTGGTATCA813311.9256171168280958No Hit
CATGTACTCTGCGTTGATACCACTG694471.6442479732495698No Hit
GCGTTGATACCACTGCTTCCCATGT612611.4504337853217835No Hit
GTATCAACGCAGAGTACATGGGAAG530171.2552463720214326No Hit
ACGCAGAGTACATGGGAAGCAGTGG494811.1715269768940626No Hit
GCTTCCCATGTACTCTGCGTTGATA411630.9745875174287161No Hit
ACTCTGCGTTGATACCACTGCTTCC399640.9461996342958775No Hit
TATCAACGCAGAGTACATGGGAAGC345070.8169980677771955No Hit
GGTATCAACGCAGAGTACATGGGAA282290.6683582593468703No Hit
CAGTGGTATCAACGCAGAGTACATG279190.6610186064935093No Hit
GTGGTATCAACGCAGAGTACATGGG241500.5717826335763547No Hit
ATACCACTGCTTCCCATGTACTCTG219350.5193396301241135No Hit
GCAGTGGTATCAACGCAGAGTACAT219180.518937133032155No Hit
CATGGGAAGCAGTGGTATCAACGCA206150.48808691474851157No Hit
GGGAAGCAGTGGTATCAACGCAGAG182570.4322582004639134No Hit
CTGCTTCCCATGTACTCTGCGTTGA180870.42823322954432835No Hit
GTACTCTGCGTTGATACCACTGCTT180600.4275939694571001No Hit
CCATGTACTCTGCGTTGATACCACT179940.42603133368832No Hit
ACATGGGAAGCAGTGGTATCAACGC178910.42359267483704194No Hit
TACCACTGCTTCCCATGTACTCTGC167090.39560728879616197No Hit
GATACCACTGCTTCCCATGTACTCT161460.3822775321624772No Hit
GTACATGGTAAGCAGTGGTATCAAC161060.3813304801813983No Hit
GCAGAGTACATGGGAAGCAGTGGTA146910.3478285163507341No Hit
AAGCAGTGGTATCAACGCAGAGTAC146420.34666837767391245No Hit
CTTCCCATGTACTCTGCGTTGATAC146370.34654999617627763No Hit
CCACTGCTTCCCATGTACTCTGCGT144310.34167267847372157No Hit
ACCATGTACTCTGCGTTGATACCAC133310.31562874899405324No Hit
GTATCAACGCAGAGTACTTTTTTTT129220.305945142487522No Hit
ATGGGAAGCAGTGGTATCAACGCAG125510.29716123536301564No Hit
GTTGATACCACTGCTTCCCATGTAC124930.29578800999045135No Hit
GTACATGGGTAAGCAGTGGTATCAA116590.27604197618495735No Hit
TTCCCATGTACTCTGCGTTGATACC96710.2289734927253386No Hit
ATCAACGCAGAGTACATGGGAAGCA96150.2276476199518282No Hit
TGATACCACTGCTTCCCATGTACTC95200.22539837149676595No Hit
GGTAAGCAGTGGTATCAACGCAGAG91740.2172063718604339No Hit
CCCCATGTACTCTGCGTTGATACCA91640.2169696088651642No Hit
TATCAACGCAGAGTACTTTTTTTTT82770.19596873118474073No Hit
GGTATCAACGCAGAGTACTTTTTTT81540.19305654634292327No Hit
GAGTACATGGTAAGCAGTGGTATCA67330.15941252471509715No Hit
GCTTACCATGTACTCTGCGTTGATA61680.14603541548235843No Hit
ACGCAGAGTACTTTTTTTTTTTTTT61030.14449645601310532No Hit
GTACTTTTTTTTTTTTTTTTTTTTT57710.13663592457015086No Hit
GGAAGCAGTGGTATCAACGCAGAGT55690.13185331206570267No Hit
CTGCGTTGATACCACTGCTTCCCAT53970.12778098854706363No Hit
AAAAAGTACTCTGCGTTGATACCAC53390.1264077631744993No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA53320.12624202907781049No Hit
GCGTTGATACCACTGCTTACCATGT48220.1141671163190552No Hit
ACTCTGCGTTGATACCACTGCTTAC47980.11359888513040788No Hit
CTCTGCGTTGATACCACTGCTTCCC46890.11101816848196802No Hit
TCCATGTACTCTGCGTTGATACCAC45380.10744304725339535No Hit
GAGTACTTTTTTTTTTTTTTTTTTT43780.10365483932907997No Hit
TGGGAAGCAGTGGTATCAACGCAGA42920.10161867756976044No Hit
TCCCATGTACTCTGCGTTGATACCA42550.1007426544872625No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG550.003037371712.1081435
AGACCGT1406.87578E-1011.53027256
GCACCGT1002.4135834E-510.445076
CGCAAAA1659.076757E-1010.37853052
TCTAGAC1855.820766E-1110.2849093
CCCGGAC856.5121095E-410.073163
CTAGACT2002.5465852E-119.9892184
GTCGAAA1151.0345633E-59.90731215
CCGTCTG1356.500195E-79.8463889
ACCGTCT1356.500195E-79.8463888
CCCACGT1951.5825208E-109.7617621
GTTCTAG2450.09.7119561
GTACTTG5050.09.6119531
GTCTAGC1502.5810004E-79.5177171
CCACGTA2002.582965E-109.5136542
TCGAAAC1304.274503E-69.49450716
GCTATAC2151.03682396E-109.2922953
GTCCTAG2054.0927262E-109.2855781
CGAAACA1252.7753713E-59.11472717
CCGTGCG1054.5205047E-49.0426039
Produced by FastQC (version 0.11.5)