FastQCFastQC Report
Wed 25 May 2016
SRR1294699_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294699_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3661458
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC1558434.2563099180708885No Hit
CCCATGTACTCTGCGTTGATACCAC1480534.043553141945094No Hit
GAGTACATGGGAAGCAGTGGTATCA731451.9977014620951545No Hit
CATGTACTCTGCGTTGATACCACTG581401.5878920364510531No Hit
GCGTTGATACCACTGCTTCCCATGT491371.3420063810645924No Hit
GTATCAACGCAGAGTACATGGGAAG407941.1141463318710743No Hit
GCTTCCCATGTACTCTGCGTTGATA399711.0916689471789653No Hit
ACGCAGAGTACATGGGAAGCAGTGG394531.0775215774699587No Hit
ACTCTGCGTTGATACCACTGCTTCC316370.8640547017062602No Hit
TATCAACGCAGAGTACATGGGAAGC312620.8538128800057245No Hit
GGTATCAACGCAGAGTACATGGGAA265860.7261041912811781No Hit
CAGTGGTATCAACGCAGAGTACATG216520.5913491292266633No Hit
GTGGTATCAACGCAGAGTACATGGG198590.5423795657358353No Hit
GCAGTGGTATCAACGCAGAGTACAT175060.47811554850554067No Hit
ATACCACTGCTTCCCATGTACTCTG167770.45820544711969935No Hit
GTACTCTGCGTTGATACCACTGCTT161310.4405622022702432No Hit
GTACATGGTAAGCAGTGGTATCAAC160470.4382680342093232No Hit
CTGCTTCCCATGTACTCTGCGTTGA149090.4071875192887642No Hit
CATGGGAAGCAGTGGTATCAACGCA148860.4065593542244647No Hit
GGGAAGCAGTGGTATCAACGCAGAG146430.3999226537625176No Hit
ACATGGGAAGCAGTGGTATCAACGC143500.3919203770738323No Hit
TACCACTGCTTCCCATGTACTCTGC127970.3495055794713472No Hit
GCAGAGTACATGGGAAGCAGTGGTA125330.3422953369941701No Hit
AAGCAGTGGTATCAACGCAGAGTAC125200.3419402871752182No Hit
GATACCACTGCTTCCCATGTACTCT121260.331179546508522No Hit
GTTGATACCACTGCTTCCCATGTAC119440.32620884904319536No Hit
CTTCCCATGTACTCTGCGTTGATAC117910.32203018578937675No Hit
CCACTGCTTCCCATGTACTCTGCGT116950.3194082794340397No Hit
CCATGTACTCTGCGTTGATACCACT115790.3162401425880073No Hit
ACCATGTACTCTGCGTTGATACCAC111850.3054794019213111No Hit
ATGGGAAGCAGTGGTATCAACGCAG97540.26639661031206696No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA93300.2548165239093279No Hit
GTACATGGGTAAGCAGTGGTATCAA93260.2547072778111889No Hit
TTCCCATGTACTCTGCGTTGATACC88120.2406691542003213No Hit
TGATACCACTGCTTCCCATGTACTC80390.2195573457349504No Hit
GGTAAGCAGTGGTATCAACGCAGAG74370.20311580796502376No Hit
CCCCATGTACTCTGCGTTGATACCA70520.19260087101914047No Hit
ATCAACGCAGAGTACATGGGAAGCA64460.17605008715107479No Hit
GCTTACCATGTACTCTGCGTTGATA59710.1630771129970629No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA58470.15969048395475244No Hit
GAGTACATGGTAAGCAGTGGTATCA55160.1506503693337463No Hit
AAAAAGTACTCTGCGTTGATACCAC46640.1273809504301292No Hit
GGAAGCAGTGGTATCAACGCAGAGT45700.12481366712386159No Hit
GTATCAACGCAGAGTACTTTTTTTT39930.109054917467304No Hit
GCGTTGATACCACTGCTTACCATGT39810.10872717917288685No Hit
ACTCTGCGTTGATACCACTGCTTAC38290.10457582744360307No Hit
GAACAAAAAAAAAAAAAAAAAAAAA38080.10400228542837306No Hit
CTGCGTTGATACCACTGCTTCCCAT37650.1028278898733783No Hit
TCCATGTACTCTGCGTTGATACCAC37140.10143500212210546No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGAC400.005285614.2480943
CGCAAAA4450.014.0883882
TATACCG500.001502201213.2976765
GTACAAA9950.013.0816911
GAACAAA18600.012.0549511
ACGCAAA5750.011.4011231
TATCGGT855.354535E-511.170855513
GTACAAG4900.011.052111
TAGGACC1408.38736E-910.8552474
AGGGCCG700.001494415310.8552475
GGTATCG1451.4317266E-810.48923917
GTCCTAG1752.3646862E-1010.3153021
GTTCTAA2400.010.292681
ATCCGAA750.00265980310.1283812
TACAAAA14400.010.02671052
ACTATCG951.6526575E-49.995522511
CTATCGG951.6533409E-49.99511212
CTAGTAC1451.6223021E-79.8262723
TACAAGA4650.09.8054082
CTAGACA2151.0913936E-119.7192324
Produced by FastQC (version 0.11.5)