##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294699_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3661458
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.008852211332208	33.0	33.0	33.0	27.0	33.0
2	30.919839036799	33.0	33.0	33.0	27.0	33.0
3	31.048318729861165	33.0	33.0	33.0	27.0	33.0
4	34.34593405140794	37.0	37.0	37.0	27.0	37.0
5	34.83197267318101	37.0	37.0	37.0	33.0	37.0
6	34.86050830024542	37.0	37.0	37.0	33.0	37.0
7	34.90249621871943	37.0	37.0	37.0	33.0	37.0
8	34.81490624772973	37.0	37.0	37.0	33.0	37.0
9	34.47298480550644	37.0	37.0	37.0	27.0	37.0
10	34.609761739722266	37.0	37.0	37.0	27.0	37.0
11	34.878658173874996	37.0	37.0	37.0	33.0	37.0
12	34.83234574860616	37.0	37.0	37.0	33.0	37.0
13	34.659419280516126	37.0	37.0	37.0	27.0	37.0
14	35.95114268687501	40.0	37.0	40.0	27.0	40.0
15	36.14752565781173	40.0	37.0	40.0	27.0	40.0
16	36.12473036697403	40.0	37.0	40.0	27.0	40.0
17	36.14834828093071	40.0	37.0	40.0	27.0	40.0
18	36.12660011394368	40.0	37.0	40.0	27.0	40.0
19	36.08343861926041	40.0	37.0	40.0	27.0	40.0
20	36.038840265271375	40.0	37.0	40.0	27.0	40.0
21	35.947479665204405	40.0	37.0	40.0	27.0	40.0
22	35.904502796426996	40.0	37.0	40.0	27.0	40.0
23	35.57400631114709	37.0	37.0	40.0	27.0	40.0
24	35.936024392468795	40.0	37.0	40.0	27.0	40.0
25	34.89030517351285	37.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.5324725275275526
1101	2	0.6757243550613303
1101	3	0.48315442084955507
1101	4	0.5807453426781279
1101	5	0.5338376729048662
1101	6	0.42859621150729765
1101	7	0.4089579888888011
1101	8	0.5449908188782189
1101	9	0.7696141888537653
1101	10	0.7714182906413498
1101	11	0.5393672470765338
1101	12	0.5604071533084678
1101	13	0.4948093329206742
1101	14	1.2740310102642667
1101	15	0.9308610535288153
1101	16	0.9479734437375598
1101	17	0.964674207777513
1101	18	0.967888970279212
1101	19	0.9114626554287
1101	20	1.0425111840506815
1101	21	0.9606749672445787
1101	22	1.0757929819580667
1101	23	1.4204780732528093
1101	24	1.1196888811465655
1101	25	1.1296854162000258
1102	1	0.45864492513231525
1102	2	0.6393239438270086
1102	3	0.5412347029532221
1102	4	0.47234265109712226
1102	5	0.423688156829229
1102	6	0.38555524818995934
1102	7	0.3708432911939852
1102	8	0.4976620173639219
1102	9	0.6699012562947004
1102	10	0.6652149452671239
1102	11	0.5499952499979202
1102	12	0.536627110129011
1102	13	0.5383450412185411
1102	14	1.197918381799795
1102	15	1.0955299139191723
1102	16	0.8142306970530129
1102	17	0.8997765001745179
1102	18	0.9245146825520152
1102	19	0.8788723500507274
1102	20	0.9127260344674966
1102	21	1.0433251650954176
1102	22	0.949564054506709
1102	23	1.3568559146266637
1102	24	1.160283905663995
1102	25	1.1137916928920077
1103	1	0.3808984859631046
1103	2	0.687266094777037
1103	3	0.42261545017307256
1103	4	0.3080469292697643
1103	5	0.3947982665165668
1103	6	0.3223848660890454
1103	7	0.2977091836841339
1103	8	0.49927350453282315
1103	9	0.7096457665572302
1103	10	0.6918591829402487
1103	11	0.5661725137283042
1103	12	0.512493939421482
1103	13	0.5223714378732538
1103	14	1.0793876477283604
1103	15	0.8514305526080648
1103	16	0.6261392178419385
1103	17	0.6955458305583164
1103	18	0.8236376347902663
1103	19	0.9109040341473431
1103	20	0.9377371250073523
1103	21	1.0089623583525622
1103	22	0.9447436312093203
1103	23	1.3395095445708165
1103	24	1.0195883988906616
1103	25	0.9555724950452102
1104	1	0.3207486296931741
1104	2	0.5148698979577233
1104	3	0.3453750675212106
1104	4	0.4423879813155338
1104	5	0.35244266418546744
1104	6	0.34181456850833314
1104	7	0.3817718639077228
1104	8	0.5347300634935692
1104	9	0.6799020571320824
1104	10	0.6703492654532042
1104	11	0.43634196037314155
1104	12	0.4962426799600905
1104	13	0.4981992041441501
1104	14	0.9746646394725786
1104	15	0.8760798669449485
1104	16	0.7656805831541789
1104	17	0.7593799339676721
1104	18	0.7566219467821327
1104	19	0.8751602394490874
1104	20	0.8149223045409144
1104	21	0.8689533373551868
1104	22	0.9038982605293597
1104	23	1.2431424034415741
1104	24	0.993444112301205
1104	25	1.0339133496164123
1105	1	0.4020391168242412
1105	2	0.40444224746783775
1105	3	0.4552207671817996
1105	4	0.4610269794885653
1105	5	0.4522353823496843
1105	6	0.30760559813882793
1105	7	0.2962142829300518
1105	8	0.30033130357019644
1105	9	-1.4759849993294551
1105	10	0.030838053684753675
1105	11	0.17102052861111616
1105	12	0.2923075643916846
1105	13	0.29188227400211275
1105	14	-1.3242370634505107
1105	15	-0.009058888109230168
1105	16	0.19126289201263802
1105	17	0.35002023332096854
1105	18	0.34666201876986236
1105	19	0.398549568543352
1105	20	0.4269737025254443
1105	21	0.6283702885697906
1105	22	0.5885628144829909
1105	23	1.0364986438216732
1105	24	0.7519053984374295
1105	25	0.5541424064448464
1106	1	0.18906393035003077
1106	2	0.18477180949437866
1106	3	0.20973639489042029
1106	4	-1.1164987717118677
1106	5	-0.22968107429483098
1106	6	0.01784231401068581
1106	7	0.07753364630534065
1106	8	0.23980128404086543
1106	9	0.42321588609725325
1106	10	0.4464436993710237
1106	11	0.3534761330203082
1106	12	0.17898106814299553
1106	13	0.30444056802827646
1106	14	0.6684118583145704
1106	15	0.34389318077560915
1106	16	0.24998362983590283
1106	17	0.1414982437293375
1106	18	0.2509904146128221
1106	19	0.3266044058413442
1106	20	0.34005847631371466
1106	21	0.5339009324073842
1106	22	0.6331093302973869
1106	23	0.899453585736758
1106	24	0.5403324810509957
1106	25	0.46968137843340685
1107	1	0.3979391667062231
1107	2	0.2975473551491028
1107	3	0.24449958793916338
1107	4	0.44278903044878604
1107	5	0.42006458382698497
1107	6	0.31475900034096327
1107	7	0.27421975219569816
1107	8	0.3463361152061495
1107	9	0.548484278952337
1107	10	0.4971634789890018
1107	11	0.3397729145578623
1107	12	0.3592230652130013
1107	13	0.3021539796603676
1107	14	0.7765197384668525
1107	15	0.5897568106886908
1107	16	0.5470083317457224
1107	17	0.6453421064332971
1107	18	0.6097052209786824
1107	19	0.5713575241994064
1107	20	0.5900536325211476
1107	21	0.7138741437479581
1107	22	0.7013272757392102
1107	23	0.9749212265567522
1107	24	0.8579779737597804
1107	25	0.731437581262874
1108	1	0.5032448535307488
1108	2	0.48654537850896773
1108	3	0.49574904995270686
1108	4	0.6598819591658369
1108	5	0.5195817208902795
1108	6	0.46163001632607603
1108	7	0.5088891307237162
1108	8	0.4290363843620142
1108	9	0.22420261094760718
1108	10	0.550307353335306
1108	11	0.3793117282001859
1108	12	0.46067967364975004
1108	13	0.4303763582762983
1108	14	-0.9470792264839218
1108	15	-0.016669398303314154
1108	16	0.4008771909915012
1108	17	0.483535677165591
1108	18	0.5925784104804421
1108	19	0.6183984550726223
1108	20	0.7228320568950579
1108	21	0.736668791126533
1108	22	0.9181234630534973
1108	23	1.1678911716363842
1108	24	0.9428089983625725
1108	25	0.9100705035117187
1109	1	0.3672486974949116
1109	2	0.29909857222694924
1109	3	0.3492977267342816
1109	4	0.34839761974152594
1109	5	0.445925105778997
1109	6	0.2372532906426983
1109	7	0.2018812827714811
1109	8	0.3466646122144894
1109	9	0.48241536983530864
1109	10	0.48333405189696066
1109	11	0.35456088080916004
1109	12	0.31555649430001154
1109	13	0.2743736729263375
1109	14	0.7076371222248099
1109	15	0.48232750069630015
1109	16	0.42301546508137733
1109	17	0.477934144423628
1109	18	0.4711910498981595
1109	19	0.44751633226084664
1109	20	0.6175524670503023
1109	21	0.5754551875572602
1109	22	0.5737514272873341
1109	23	1.0405578908976167
1109	24	0.7918524544476995
1109	25	0.6617428380194283
1110	1	0.35298085192082596
1110	2	0.15599104971116518
1110	3	0.24735637388159404
1110	4	0.5489087166703683
1110	5	0.48772797699913184
1110	6	0.2299296782728959
1110	7	0.28418164434077653
1110	8	0.29789800341765726
1110	9	0.4228208925086605
1110	10	0.44514360814513054
1110	11	0.23041240258265816
1110	12	0.24768877995534666
1110	13	0.15477430273811166
1110	14	0.6041621314489305
1110	15	0.4957666899168913
1110	16	0.30946506857778644
1110	17	0.46104980052747635
1110	18	0.4379996091581262
1110	19	0.3697519287230904
1110	20	0.4416504163023376
1110	21	0.5199609529379572
1110	22	0.6063815657260179
1110	23	0.8260825458658516
1110	24	0.729241418526648
1110	25	0.6449685482065775
1111	1	0.20627872615545328
1111	2	0.06469407101897318
1111	3	0.2654040845031034
1111	4	0.45686496597009807
1111	5	0.2666534050855276
1111	6	0.19345727843615634
1111	7	0.17034275415384315
1111	8	0.23353872017450783
1111	9	0.24810232109864927
1111	10	0.1503983903113877
1111	11	0.1257371924419317
1111	12	0.116964804145411
1111	13	0.18053191119896894
1111	14	-1.7692575942672946
1111	15	-0.6104361580591942
1111	16	0.006379660955687427
1111	17	0.05024120677814636
1111	18	0.0982800635981036
1111	19	0.14105159140898138
1111	20	0.3472097641949219
1111	21	0.4342932044485437
1111	22	0.2345727228713841
1111	23	-1.75166744985453
1111	24	-0.27274873302567926
1111	25	0.05678049070751001
1112	1	-0.1024960894391782
1112	2	-0.25631910755544496
1112	3	-0.006191336813994752
1112	4	0.11954513101099451
1112	5	-0.689405496431263
1112	6	-0.8247773034652752
1112	7	-0.9219514706134788
1112	8	-0.7013650116528396
1112	9	-0.519256843189126
1112	10	-0.7694257267579943
1112	11	-0.8489142101890366
1112	12	-0.6882931665395233
1112	13	-0.7720844232662571
1112	14	-0.6018214471098275
1112	15	-0.8609922523567803
1112	16	-0.8360103663044498
1112	17	-0.8712567736448307
1112	18	-0.9114092108441199
1112	19	-0.8490213460389455
1112	20	-0.8615066187515836
1112	21	-1.92898242600333
1112	22	-1.7743945553954603
1112	23	-1.3548980583578754
1112	24	-1.5371581701732495
1112	25	-1.8319615590600833
1113	1	-0.14462338599900804
1113	2	-0.2558876893725781
1113	3	-0.12194809624652336
1113	4	0.009078544296258428
1113	5	-0.012244096635079416
1113	6	-0.1666302625806111
1113	7	-0.1873034389509911
1113	8	0.023546378020384395
1113	9	0.11487393185205264
1113	10	-0.23993818795950972
1113	11	-0.2787738816200971
1113	12	-0.1733996992708029
1113	13	-0.3018330919648662
1113	14	-0.21499287743176154
1113	15	-0.31990221442801925
1113	16	-0.29791481576290835
1113	17	-0.17831813530236929
1113	18	-0.12805433133487298
1113	19	-0.3515256384908696
1113	20	-0.798784085938891
1113	21	-0.6962065584552803
1113	22	-0.20731681892689124
1113	23	-0.33446637941610646
1113	24	-0.06355253600018074
1113	25	-1.1386852061400816
1114	1	-1.0987116403792783
1114	2	-0.904492491449151
1114	3	-0.6696443973497033
1114	4	-1.7350810923033535
1114	5	-0.8848917280432573
1114	6	-0.8029054085896945
1114	7	-0.6903858529678644
1114	8	-0.6286275350230923
1114	9	-0.6406419669149841
1114	10	-0.749996591593515
1114	11	-0.7432450042677345
1114	12	-0.7991297446958541
1114	13	-0.822274363866228
1114	14	-0.863597679508338
1114	15	-1.5012779444734434
1114	16	-1.1929505068725774
1114	17	-1.1239703092154159
1114	18	-1.1016104637164474
1114	19	-0.9404289480301316
1114	20	-2.07344339807387
1114	21	-6.846109219065276
1114	22	-7.59326750030262
1114	23	-7.930507910889176
1114	24	-6.020885436714
1114	25	-7.531611877132946
1115	1	-1.1327312850819204
1115	2	-1.0709795437652119
1115	3	-1.0054558507795868
1115	4	-0.7752812303295755
1115	5	-0.7619430226399757
1115	6	-0.9593930703499041
1115	7	-1.175531617416837
1115	8	-0.9345085208805557
1115	9	-2.826859022595862
1115	10	-1.7544437098668766
1115	11	-1.312591808891952
1115	12	-1.2835841977583513
1115	13	-1.5922554410544763
1115	14	-1.3645152792254152
1115	15	-2.184541341944339
1115	16	-1.5295916841813408
1115	17	-3.2532967189198274
1115	18	-2.910528799058369
1115	19	-2.3056764467339903
1115	20	-1.9798062739979088
1115	21	-1.3794479601424996
1115	22	-0.9339064381187114
1115	23	-0.29595541027973127
1115	24	-1.414152452223064
1115	25	-1.1488955416262172
1116	1	-1.5292676191182544
1116	2	-0.856173516459183
1116	3	-0.7329690782928147
1116	4	-0.38644784149619227
1116	5	-0.38682971002666733
1116	6	-0.4914397273965605
1116	7	-0.8267172373524616
1116	8	-1.7543796220016574
1116	9	-0.8149877919746302
1116	10	-2.5183684425416146
1116	11	-1.2169906507907982
1116	12	-0.9766920158661705
1116	13	-0.835691403240439
1116	14	-1.2475396622497996
1116	15	-1.303899992552985
1116	16	-0.8732399340795922
1116	17	-0.7302063833294881
1116	18	-1.2078252526048203
1116	19	-0.8790602388677797
1116	20	-1.094623170064807
1116	21	-0.6691166248111671
1116	22	-0.24345799767027643
1116	23	-0.15117773425205883
1116	24	-0.2316913947620094
1116	25	-0.29169718442786063
1201	1	0.2714891019447627
1201	2	0.3606389771542169
1201	3	0.3352857903023825
1201	4	0.37743915069482625
1201	5	0.2626421076243943
1201	6	0.16903422859639505
1201	7	0.267243538117107
1201	8	0.3385258654018415
1201	9	0.6088370124514171
1201	10	0.4844161617744689
1201	11	0.3347987396259384
1201	12	0.3793441770957173
1201	13	0.3997856362638288
1201	14	1.0249528291876757
1201	15	0.8433248756021143
1201	16	0.7881877854876649
1201	17	0.7132400360102906
1201	18	0.6993446242190515
1201	19	0.740470636760655
1201	20	0.8078076379247108
1201	21	0.7586452521849694
1201	22	0.7821191855926699
1201	23	0.7056690491403899
1201	24	0.7401686604786448
1201	25	0.8269753134278517
1202	1	0.038907449056811316
1202	2	0.2055485452628787
1202	3	0.13252578720205221
1202	4	0.19628924124089053
1202	5	0.28716746397051196
1202	6	0.15351891756208147
1202	7	0.16530425466903154
1202	8	0.29423392661188785
1202	9	0.3385253615385224
1202	10	0.5227300985569272
1202	11	0.31089137709123094
1202	12	0.27057873140457644
1202	13	0.4619343943853593
1202	14	0.7013762866661466
1202	15	0.6167019280489328
1202	16	0.5982660374263844
1202	17	0.4452802921571859
1202	18	0.34105379627422394
1202	19	0.45456559475805136
1202	20	0.5829380074963737
1202	21	0.5497005940060546
1202	22	0.48586673165445404
1202	23	0.7391886561143366
1202	24	0.41821725514663655
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2211	5	0.2605245613150444
2211	6	0.23056934855531352
2211	7	0.22193282311773288
2211	8	0.2673593432816972
2211	9	0.46944222117087264
2211	10	0.3774951857571267
2211	11	0.21086669052331786
2211	12	0.3022025342302328
2211	13	0.21017580058676089
2211	14	0.5277591008155369
2211	15	0.4064649166877672
2211	16	0.18992886383875174
2211	17	0.2681248440478612
2211	18	0.3154178843761244
2211	19	0.3152831099639144
2211	20	0.3863525963300418
2211	21	0.3577606693830404
2211	22	0.376885454546219
2211	23	0.5732258998060686
2211	24	0.5171472466208513
2211	25	0.7162402410165924
2212	1	-0.23035420376648474
2212	2	-0.38386500305477966
2212	3	-0.22463722749520798
2212	4	-0.46014355217820224
2212	5	-0.2453159726225138
2212	6	-0.49885240604211845
2212	7	-0.4589411777955803
2212	8	-0.30350857524020114
2212	9	-0.22826401347003156
2212	10	-0.4746017069940578
2212	11	-0.40670276516044623
2212	12	-0.47252266923106845
2212	13	-0.5791100850152802
2212	14	-0.43906047020896466
2212	15	-0.6429399480312981
2212	16	-0.3650608985032946
2212	17	-0.5284697637846492
2212	18	-0.3888930432616533
2212	19	-0.697727872603906
2212	20	-0.5361428376118695
2212	21	-0.5708952115212611
2212	22	-0.618931838897538
2212	23	-0.060124951418451644
2212	24	-0.27706154972120345
2212	25	-0.9452148995894092
2213	1	-0.12090531926248005
2213	2	-1.1532671107978238
2213	3	-0.20437233268301824
2213	4	0.0629467304213378
2213	5	0.061823698752405676
2213	6	-0.008507805121965362
2213	7	0.059480743818937754
2213	8	0.12940237657009135
2213	9	0.19261407932950902
2213	10	-0.29437566393307435
2213	11	-0.08967033134867819
2213	12	0.016304113186023983
2213	13	-0.3086810364523984
2213	14	-0.057206071239555456
2213	15	-0.1090619644621782
2213	16	-0.06908138922505458
2213	17	0.16390425872701542
2213	18	0.07295542957750456
2213	19	0.08457858726500689
2213	20	0.09419180101212987
2213	21	-0.406091266087401
2213	22	-0.7462400553097481
2213	23	-0.007609766856148781
2213	24	0.19059519572177663
2213	25	-0.44085469680431544
2214	1	-0.3652858278462041
2214	2	-0.6795717511399637
2214	3	-0.6127504990578423
2214	4	-0.6809079618965228
2214	5	-0.39860064561630537
2214	6	-0.3670779111350342
2214	7	-0.6762295058618335
2214	8	-0.3428698845517104
2214	9	-0.3484336132700179
2214	10	-2.1067230323426855
2214	11	-1.0418299066811585
2214	12	-0.7411917398644476
2214	13	-0.4903999219798152
2214	14	-0.9505030440945674
2214	15	-0.796329613719351
2214	16	-0.8728761063290662
2214	17	-0.7019061718084529
2214	18	-0.8194392894840448
2214	19	-1.6303183025655699
2214	20	-2.588335500807027
2214	21	-1.9727861150766017
2214	22	-2.1937027063527808
2214	23	-2.666105268948151
2214	24	-1.8038749168008863
2214	25	-2.181858453566946
2215	1	-0.23087595230553504
2215	2	-0.36465934512045095
2215	3	-0.876568818383781
2215	4	-0.37641369111116063
2215	5	-0.23840617125225094
2215	6	-0.18065223678451048
2215	7	-0.17433940076409726
2215	8	-0.19544732622726002
2215	9	-0.10638596495175534
2215	10	-0.32922847269988864
2215	11	-0.49952325788480323
2215	12	-0.35314467302058006
2215	13	-0.21442718987096043
2215	14	-0.16865525880399446
2215	15	-0.6491838509947385
2215	16	-0.47110925124714953
2215	17	-0.24070157364923972
2215	18	-0.26316675970071657
2215	19	-2.191493419722015
2215	20	-2.4311284451665713
2215	21	-3.1871964981541936
2215	22	-2.3496216520795414
2215	23	-3.4794842555410312
2215	24	-2.2990382778983687
2215	25	-1.8069063092861057
2216	1	-1.5356243410810642
2216	2	-1.2860797790171716
2216	3	-0.9043524688811786
2216	4	-1.1149931115922556
2216	5	-1.940592295115934
2216	6	-1.6172740326844917
2216	7	-1.2286878334176095
2216	8	-5.095899626616706
2216	9	-4.063207046410145
2216	10	-2.09463761205469
2216	11	-1.1787737011555564
2216	12	-1.332078191389762
2216	13	-2.0939849119020124
2216	14	-3.643935839277418
2216	15	-2.0910036680911475
2216	16	-3.2587448539375288
2216	17	-2.8254974001811135
2216	18	-2.8482276823905366
2216	19	-1.8518921672861595
2216	20	-0.9915557538123707
2216	21	-0.8524767512434295
2216	22	-0.7100048648387656
2216	23	-1.0610374639220268
2216	24	-0.6392347211667797
2216	25	-0.4890864955910956
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	1.0
5	0.0
6	9.0
7	59.0
8	280.0
9	715.0
10	1355.0
11	2630.0
12	4495.0
13	7095.0
14	10770.0
15	16524.0
16	21596.0
17	23738.0
18	23855.0
19	24073.0
20	24435.0
21	26181.0
22	26685.0
23	27136.0
24	28884.0
25	30416.0
26	31646.0
27	34381.0
28	39006.0
29	45319.0
30	53831.0
31	66004.0
32	89461.0
33	129365.0
34	190366.0
35	307356.0
36	548913.0
37	1824878.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.05256184204128	19.11881135643516	10.841351184332531	23.98727561719103
2	12.243281322642378	23.506698054092503	36.34242705510154	27.90759356816358
3	21.592507492589363	29.801369550542983	26.776059882801178	21.830063074066473
4	13.174870420672155	19.961977861488283	32.60574582864249	34.257405889197074
5	11.407701278829393	41.07771242771053	34.3038860994679	13.210700193992169
6	32.38043705088438	32.81399716586672	18.40703463782858	16.398531145420318
7	23.814702792819418	34.35541518109053	22.799248795106024	19.030633230984034
8	26.679476201986436	36.230801060269	21.53023419053433	15.55948854721024
9	26.927562467668885	18.277026897846525	20.11611068534037	34.67929994914422
10	16.61045372298658	32.454692567682905	31.302229353726403	19.63262435560411
11	28.317187768427015	28.79962921054019	19.01322917579916	23.86995384523364
12	23.441961684969858	32.29511908478299	27.92816266421732	16.33475656602983
13	28.38302847823908	23.16979742756579	22.362610365501098	26.084563728694032
14	21.184665411416656	27.638141683750195	25.40695937261534	25.770233532217812
15	31.6753665019561	24.769567255116385	21.110802180166342	22.444264062761174
16	18.719193524819293	29.57667427311945	31.36734523872927	20.33678696333198
17	25.79837688993671	30.33373336618082	26.930850398457846	16.93703934542463
18	25.804837546703958	26.37230428037669	28.344576878536937	19.478281294382416
19	22.852055869775935	31.735192928034113	28.963799245270238	16.448951956919718
20	23.758616150266644	28.000938429109002	26.85622424850564	21.384221172118714
21	19.036750349265947	30.533292404548778	30.116988716611992	20.31296852957328
22	21.60361734886463	29.76192200768001	22.109577156889088	26.524883486566274
23	17.530593429577372	31.177049249333137	27.257487740351564	24.034869580737926
24	20.667666281569122	35.79539030045802	23.95809264736809	19.578850770604767
25	22.225516371603046	26.82830081296139	21.66016368728649	29.28601912814907
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	12402.0
1	12402.0
2	15145.0
3	17888.0
4	17888.0
5	17888.0
6	21407.0
7	24926.0
8	24926.0
9	24926.0
10	25859.0
11	26792.0
12	26792.0
13	26792.0
14	31092.0
15	35392.0
16	35392.0
17	35392.0
18	45913.5
19	56435.0
20	56435.0
21	56435.0
22	73281.5
23	90128.0
24	90128.0
25	90128.0
26	112054.0
27	133980.0
28	133980.0
29	133980.0
30	156744.0
31	179508.0
32	179508.0
33	179508.0
34	200075.5
35	220643.0
36	220643.0
37	220643.0
38	242845.5
39	265048.0
40	265048.0
41	265048.0
42	312228.0
43	359408.0
44	359408.0
45	359408.0
46	650558.0
47	941708.0
48	941708.0
49	941708.0
50	800906.5
51	660105.0
52	660105.0
53	660105.0
54	471301.5
55	282498.0
56	282498.0
57	282498.0
58	214806.5
59	147115.0
60	147115.0
61	147115.0
62	123598.0
63	100081.0
64	100081.0
65	100081.0
66	79101.0
67	58121.0
68	58121.0
69	58121.0
70	43720.0
71	29319.0
72	29319.0
73	29319.0
74	20916.5
75	12514.0
76	12514.0
77	12514.0
78	8721.5
79	4929.0
80	4929.0
81	4929.0
82	3337.5
83	1746.0
84	1746.0
85	1746.0
86	1145.5
87	545.0
88	545.0
89	545.0
90	363.0
91	181.0
92	181.0
93	181.0
94	105.5
95	30.0
96	30.0
97	30.0
98	23.0
99	16.0
100	16.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.029605692595681826
2	0.014857469346910438
3	0.022968992133734704
4	0.026191752028836602
5	0.027338836059296597
6	0.010214510176000927
7	0.003823613434866657
8	0.0033593175177757052
9	0.0033866290423104676
10	0.002266856536385232
11	0.0022122334873157087
12	0.0020483643401071378
13	0.0027584639780109453
14	9.83214883251426E-4
15	0.0023487911099895177
16	0.002539971781732851
17	0.0025126602571980887
18	0.0026765294044066596
19	4.0967286802142755E-4
20	5.462304906952367E-4
21	0.0838736918462536
22	0.07830214084116219
23	0.0795038479206917
24	0.0013109531776685681
25	0.23261225446256656
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3661458.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.48666896868408
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.66693809341307	41.15524199771342
2	9.146350518013854	8.68659438640952
3	1.9470120679519851	2.7737135264660684
4	0.7120454362722104	1.3525066369168277
5	0.386012108738294	0.9165214612779524
6	0.2188464606864388	0.6235373660151032
7	0.1510648250375056	0.5021495737557746
8	0.11028162992645929	0.41895258029157567
9	0.07944759463951596	0.3395431464304404
>10	0.5008634321879061	4.423869368684727
>50	0.04232743872737889	1.4008756248988146
>100	0.02898809946433032	2.7909516902460094
>500	0.0035664285202510904	1.2702113426369737
>1k	0.0038587587268209887	4.124695593945212
>5k	6.431264544701648E-4	2.3190914613956424
>10k+	0.0017539812394640857	26.901544242915946
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	155843	4.2563099180708885	No Hit
CCCATGTACTCTGCGTTGATACCAC	148053	4.043553141945094	No Hit
GAGTACATGGGAAGCAGTGGTATCA	73145	1.9977014620951545	No Hit
CATGTACTCTGCGTTGATACCACTG	58140	1.5878920364510531	No Hit
GCGTTGATACCACTGCTTCCCATGT	49137	1.3420063810645924	No Hit
GTATCAACGCAGAGTACATGGGAAG	40794	1.1141463318710743	No Hit
GCTTCCCATGTACTCTGCGTTGATA	39971	1.0916689471789653	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	39453	1.0775215774699587	No Hit
ACTCTGCGTTGATACCACTGCTTCC	31637	0.8640547017062602	No Hit
TATCAACGCAGAGTACATGGGAAGC	31262	0.8538128800057245	No Hit
GGTATCAACGCAGAGTACATGGGAA	26586	0.7261041912811781	No Hit
CAGTGGTATCAACGCAGAGTACATG	21652	0.5913491292266633	No Hit
GTGGTATCAACGCAGAGTACATGGG	19859	0.5423795657358353	No Hit
GCAGTGGTATCAACGCAGAGTACAT	17506	0.47811554850554067	No Hit
ATACCACTGCTTCCCATGTACTCTG	16777	0.45820544711969935	No Hit
GTACTCTGCGTTGATACCACTGCTT	16131	0.4405622022702432	No Hit
GTACATGGTAAGCAGTGGTATCAAC	16047	0.4382680342093232	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	14909	0.4071875192887642	No Hit
CATGGGAAGCAGTGGTATCAACGCA	14886	0.4065593542244647	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	14643	0.3999226537625176	No Hit
ACATGGGAAGCAGTGGTATCAACGC	14350	0.3919203770738323	No Hit
TACCACTGCTTCCCATGTACTCTGC	12797	0.3495055794713472	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	12533	0.3422953369941701	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	12520	0.3419402871752182	No Hit
GATACCACTGCTTCCCATGTACTCT	12126	0.331179546508522	No Hit
GTTGATACCACTGCTTCCCATGTAC	11944	0.32620884904319536	No Hit
CTTCCCATGTACTCTGCGTTGATAC	11791	0.32203018578937675	No Hit
CCACTGCTTCCCATGTACTCTGCGT	11695	0.3194082794340397	No Hit
CCATGTACTCTGCGTTGATACCACT	11579	0.3162401425880073	No Hit
ACCATGTACTCTGCGTTGATACCAC	11185	0.3054794019213111	No Hit
ATGGGAAGCAGTGGTATCAACGCAG	9754	0.26639661031206696	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	9330	0.2548165239093279	No Hit
GTACATGGGTAAGCAGTGGTATCAA	9326	0.2547072778111889	No Hit
TTCCCATGTACTCTGCGTTGATACC	8812	0.2406691542003213	No Hit
TGATACCACTGCTTCCCATGTACTC	8039	0.2195573457349504	No Hit
GGTAAGCAGTGGTATCAACGCAGAG	7437	0.20311580796502376	No Hit
CCCCATGTACTCTGCGTTGATACCA	7052	0.19260087101914047	No Hit
ATCAACGCAGAGTACATGGGAAGCA	6446	0.17605008715107479	No Hit
GCTTACCATGTACTCTGCGTTGATA	5971	0.1630771129970629	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	5847	0.15969048395475244	No Hit
GAGTACATGGTAAGCAGTGGTATCA	5516	0.1506503693337463	No Hit
AAAAAGTACTCTGCGTTGATACCAC	4664	0.1273809504301292	No Hit
GGAAGCAGTGGTATCAACGCAGAGT	4570	0.12481366712386159	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3993	0.109054917467304	No Hit
GCGTTGATACCACTGCTTACCATGT	3981	0.10872717917288685	No Hit
ACTCTGCGTTGATACCACTGCTTAC	3829	0.10457582744360307	No Hit
GAACAAAAAAAAAAAAAAAAAAAAA	3808	0.10400228542837306	No Hit
CTGCGTTGATACCACTGCTTCCCAT	3765	0.1028278898733783	No Hit
TCCATGTACTCTGCGTTGATACCAC	3714	0.10143500212210546	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	2.7311524534761834E-5	0.0	0.0	5.462304906952367E-5	0.0
10	2.7311524534761834E-5	0.0	0.0	1.3655762267380918E-4	0.0
11	2.7311524534761834E-5	0.0	0.0	1.3655762267380918E-4	0.0
12	2.7311524534761834E-5	0.0	0.0	2.458037208128565E-4	0.0
13	2.7311524534761834E-5	0.0	0.0	3.5504981895190387E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACGAC	40	0.0052856	14.248094	3
CGCAAAA	445	0.0	14.088388	2
TATACCG	50	0.0015022012	13.297676	5
GTACAAA	995	0.0	13.081691	1
GAACAAA	1860	0.0	12.054951	1
ACGCAAA	575	0.0	11.401123	1
TATCGGT	85	5.354535E-5	11.1708555	13
GTACAAG	490	0.0	11.05211	1
TAGGACC	140	8.38736E-9	10.855247	4
AGGGCCG	70	0.0014944153	10.855247	5
GGTATCG	145	1.4317266E-8	10.489239	17
GTCCTAG	175	2.3646862E-10	10.315302	1
GTTCTAA	240	0.0	10.29268	1
ATCCGAA	75	0.002659803	10.12838	12
TACAAAA	1440	0.0	10.0267105	2
ACTATCG	95	1.6526575E-4	9.9955225	11
CTATCGG	95	1.6533409E-4	9.995112	12
CTAGTAC	145	1.6223021E-7	9.826272	3
TACAAGA	465	0.0	9.805408	2
CTAGACA	215	1.0913936E-11	9.719232	4
>>END_MODULE