FastQCFastQC Report
Wed 25 May 2016
SRR1294699_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294699_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3661458
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC1641824.4840607211662675No Hit
GTACATGGGAAGCAGTGGTATCAAC1523844.1618393547051475No Hit
GAGTACATGGGAAGCAGTGGTATCA798052.1795962154966686No Hit
CATGTACTCTGCGTTGATACCACTG661531.8067392825480997No Hit
GCGTTGATACCACTGCTTCCCATGT528541.4435233177603022No Hit
ACGCAGAGTACATGGGAAGCAGTGG478931.3080308445433486No Hit
GCTTCCCATGTACTCTGCGTTGATA443101.210173652135297No Hit
GTATCAACGCAGAGTACATGGGAAG430581.175979623417775No Hit
ACTCTGCGTTGATACCACTGCTTCC366561.0011312433462298No Hit
TATCAACGCAGAGTACATGGGAAGC352760.9634413394882585No Hit
GGTATCAACGCAGAGTACATGGGAA289010.7893303705791518No Hit
CAGTGGTATCAACGCAGAGTACATG257590.7035175604909301No Hit
GTGGTATCAACGCAGAGTACATGGG208580.5696637787460623No Hit
GCAGTGGTATCAACGCAGAGTACAT199310.5443459955023381No Hit
CATGGGAAGCAGTGGTATCAACGCA189830.518454670243384No Hit
ATACCACTGCTTCCCATGTACTCTG186400.5090868173279606No Hit
ACATGGGAAGCAGTGGTATCAACGC178660.48794769733805493No Hit
GGGAAGCAGTGGTATCAACGCAGAG167030.45618439430412694No Hit
CTGCTTCCCATGTACTCTGCGTTGA163580.44676191833963413No Hit
GTACTCTGCGTTGATACCACTGCTT158740.43354314046480935No Hit
GTACATGGTAAGCAGTGGTATCAAC153610.41953232837847654No Hit
AAGCAGTGGTATCAACGCAGAGTAC148560.40574000848842184No Hit
TACCACTGCTTCCCATGTACTCTGC146380.3997860961398437No Hit
CTTCCCATGTACTCTGCGTTGATAC141410.38621226844606715No Hit
CCATGTACTCTGCGTTGATACCACT138960.37952094493505045No Hit
GATACCACTGCTTCCCATGTACTCT138210.3774725805949433No Hit
GCAGAGTACATGGGAAGCAGTGGTA137680.37602506979460093No Hit
CCACTGCTTCCCATGTACTCTGCGT136880.37384014783181996No Hit
ACCATGTACTCTGCGTTGATACCAC129360.3533018813816791No Hit
GTTGATACCACTGCTTCCCATGTAC127440.3480580686710048No Hit
ATGGGAAGCAGTGGTATCAACGCAG121100.3307425621159658No Hit
GTATCAACGCAGAGTACTTTTTTTT113570.31017698414129014No Hit
GTACATGGGTAAGCAGTGGTATCAA101740.2778674506166669No Hit
TTCCCATGTACTCTGCGTTGATACC92800.2534509476825898No Hit
TATCAACGCAGAGTACTTTTTTTTT86940.23744639430521938No Hit
GGTAAGCAGTGGTATCAACGCAGAG86640.23662704856917652No Hit
TGATACCACTGCTTCCCATGTACTC86360.2358623258822032No Hit
GGTATCAACGCAGAGTACTTTTTTT85310.23299461580605324No Hit
CCCCATGTACTCTGCGTTGATACCA82090.2242003049058599No Hit
ATCAACGCAGAGTACATGGGAAGCA76150.20797725933221137No Hit
GTACTTTTTTTTTTTTTTTTTTTTT67000.1829872143829043No Hit
GCTTACCATGTACTCTGCGTTGATA66340.18118465376361No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA66240.1809115385182624No Hit
ACGCAGAGTACTTTTTTTTTTTTTT62310.17017810937610098No Hit
GAGTACATGGTAAGCAGTGGTATCA62160.16976843650807957No Hit
GGAAGCAGTGGTATCAACGCAGAGT54360.14846544737096534No Hit
AAAAAGTACTCTGCGTTGATACCAC51590.1409001550748363No Hit
GAGTACTTTTTTTTTTTTTTTTTTT48500.1324608939935949No Hit
ACTCTGCGTTGATACCACTGCTTAC44320.12104467673806445No Hit
CTGCGTTGATACCACTGCTTCCCAT43070.11763073617121922No Hit
GCGTTGATACCACTGCTTACCATGT42730.11670214433703732No Hit
ATGGTAAGCAGTGGTATCAACGCAG40820.1114856431508978No Hit
CTCTGCGTTGATACCACTGCTTCCC40110.10954652490892973No Hit
TCCATGTACTCTGCGTTGATACCAC39780.1086452445992826No Hit
TGGGAAGCAGTGGTATCAACGCAGA38860.1061325843420845No Hit
ACGCAGAGTACATGGTAAGCAGTGG38120.1041115315265121No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGA500.001507957613.29092911
ACGTACT1207.221388E-1012.6880034
CTACGCA905.302336E-712.6880024
CCGTGCG701.0985146E-412.2061219
CGCAAAA1950.012.2000022
TTTCGTC550.00308490612.08299317
TCTACGC1052.6647285E-711.7817163
TATTCCG907.3295687E-611.6306695
GGTACTC1850.011.3163255
CACGTAC1408.1472535E-910.875433
ACGCAAA2900.010.8330021
GTACAAA5450.010.829991
ATTAACG803.715079E-410.7055023
TCGCGTT803.7971706E-410.6802110
ATTGGAC1303.758214E-710.2480023
TACGCAG1151.0070129E-59.929745
GAACAAA9950.09.8547811
CCACGTA1659.958967E-99.8043652
CTTGTAC1752.5029294E-99.7878873
GTAATAC1951.5825208E-109.7600013
Produced by FastQC (version 0.11.5)