FastQCFastQC Report
Wed 25 May 2016
SRR1294698_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294698_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3084805
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC1147293.7191653929502833No Hit
CCCATGTACTCTGCGTTGATACCAC1075293.485763281633685No Hit
GAGTACATGGGAAGCAGTGGTATCA539191.747890061122178No Hit
CATGTACTCTGCGTTGATACCACTG411141.3327908895375882No Hit
GCGTTGATACCACTGCTTCCCATGT376991.2220869714617293No Hit
GCTTCCCATGTACTCTGCGTTGATA354521.1492460625550074No Hit
GTATCAACGCAGAGTACATGGGAAG303690.98447065535747No Hit
ACGCAGAGTACATGGGAAGCAGTGG285690.9261201275283203No Hit
ACTCTGCGTTGATACCACTGCTTCC223990.7261074849139573No Hit
TATCAACGCAGAGTACATGGGAAGC223110.72325479244231No Hit
GGTATCAACGCAGAGTACATGGGAA191300.6201364429842404No Hit
CAGTGGTATCAACGCAGAGTACATG153960.49909151469866003No Hit
GTGGTATCAACGCAGAGTACATGGG146020.47335244853402403No Hit
GCAGTGGTATCAACGCAGAGTACAT137640.44618703613356436No Hit
ATACCACTGCTTCCCATGTACTCTG116950.3791163460899473No Hit
CATGGGAAGCAGTGGTATCAACGCA111050.35999033974594824No Hit
ACATGGGAAGCAGTGGTATCAACGC110360.35775356951249754No Hit
GTACTCTGCGTTGATACCACTGCTT110260.3574293999134467No Hit
CTGCTTCCCATGTACTCTGCGTTGA107330.34793123066125736No Hit
GTACATGGTAAGCAGTGGTATCAAC106720.3459537961070473No Hit
GGGAAGCAGTGGTATCAACGCAGAG105360.341545089559956No Hit
GCAGAGTACATGGGAAGCAGTGGTA97180.315028016357598No Hit
GATACCACTGCTTCCCATGTACTCT88500.2868900951599858No Hit
TACCACTGCTTCCCATGTACTCTGC88270.28614450508216893No Hit
CTTCCCATGTACTCTGCGTTGATAC87590.28394015180862325No Hit
AAGCAGTGGTATCAACGCAGAGTAC85190.2761600814314033No Hit
CCATGTACTCTGCGTTGATACCACT82340.2669212478584546No Hit
GTTGATACCACTGCTTCCCATGTAC81870.26539765074291566No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA80880.26218837171231246No Hit
CCACTGCTTCCCATGTACTCTGCGT79690.25833075348360757No Hit
GTACATGGGTAAGCAGTGGTATCAA74820.24254369400983208No Hit
ATGGGAAGCAGTGGTATCAACGCAG74570.24173327001220496No Hit
ACCATGTACTCTGCGTTGATACCAC72200.2340504505147003No Hit
TTCCCATGTACTCTGCGTTGATACC65920.21369259969430807No Hit
CCCCATGTACTCTGCGTTGATACCA59680.19346441671353617No Hit
TGATACCACTGCTTCCCATGTACTC58220.18873154056739405No Hit
GGTAAGCAGTGGTATCAACGCAGAG51220.16603966863383585No Hit
ATCAACGCAGAGTACATGGGAAGCA46930.15213279283455516No Hit
GCTTACCATGTACTCTGCGTTGATA44560.14444997333705048No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA39370.12762557114631232No Hit
AAAAAGTACTCTGCGTTGATACCAC36230.11744664573611623No Hit
GAGTACATGGTAAGCAGTGGTATCA35730.11582579774086206No Hit
GGAAGCAGTGGTATCAACGCAGAGT34720.11255168479044866No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACGGC350.002176811216.2790326
TCCAACG400.005284250714.2485422
TGATACG853.9207007E-612.30143517
CGCAAAA3100.012.2568092
GTACAAA8950.012.1026551
CGCTCAT550.00307869912.086143510
GGTATCG951.0334115E-612.00714217
ACGCAAA3700.011.2992551
GAAGCGC600.005895153611.07896510
GATACGA951.3523391E-511.00654718
TGGACGG909.5498384E-510.5524215
AAGCGGT1355.727088E-810.55139510
ATACGAC1002.3491357E-510.4704919
GTGTTAC1553.6561687E-910.4211611
GTATCGA1106.007489E-610.36980518
GTCTAGG1856.002665E-1110.272051
CCAGGAC3550.010.1674793
TGCGTAG750.00265715510.12950312
GTCCTAG2201.8189894E-129.9335391
CTTACAC3000.09.8151843
Produced by FastQC (version 0.11.5)