##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294698_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3084805
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.86902997110028	33.0	33.0	33.0	27.0	33.0
2	30.889561252656165	33.0	33.0	33.0	27.0	33.0
3	30.969100477988075	33.0	33.0	33.0	27.0	33.0
4	34.305944135853	37.0	37.0	37.0	27.0	37.0
5	34.73558263812461	37.0	37.0	37.0	27.0	37.0
6	34.778849554509925	37.0	37.0	37.0	27.0	37.0
7	34.84029655034921	37.0	37.0	37.0	33.0	37.0
8	34.72705892268717	37.0	37.0	37.0	27.0	37.0
9	34.42970203951303	37.0	37.0	37.0	27.0	37.0
10	34.562802511017715	37.0	37.0	37.0	27.0	37.0
11	34.8054314616321	37.0	37.0	37.0	33.0	37.0
12	34.75508370869471	37.0	37.0	37.0	27.0	37.0
13	34.58732691369471	37.0	37.0	37.0	27.0	37.0
14	35.83433345057467	40.0	37.0	40.0	27.0	40.0
15	36.03399696253086	40.0	37.0	40.0	27.0	40.0
16	36.027328469708785	40.0	37.0	40.0	27.0	40.0
17	36.029506241075204	40.0	37.0	40.0	27.0	40.0
18	36.011398775611426	40.0	37.0	40.0	27.0	40.0
19	36.00302774405513	40.0	37.0	40.0	27.0	40.0
20	35.93737853770335	40.0	37.0	40.0	27.0	40.0
21	35.85569266128653	40.0	37.0	40.0	27.0	40.0
22	35.77955786508385	37.0	37.0	40.0	27.0	40.0
23	35.45395381555722	37.0	37.0	40.0	27.0	40.0
24	35.79245949095648	37.0	37.0	40.0	27.0	40.0
25	34.679297394811016	37.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.5746189973177565
1101	2	0.5832525883145436
1101	3	0.4879703304920078
1101	4	0.5801073739120781
1101	5	0.5442072614688485
1101	6	0.44743064398139865
1101	7	0.4991169163705038
1101	8	0.5171745988406258
1101	9	0.716847394032115
1101	10	0.736362107263794
1101	11	0.6242978787056899
1101	12	0.5586548289935465
1101	13	0.6197085258100046
1101	14	1.318499499061943
1101	15	1.088472847586317
1101	16	1.002456222155665
1101	17	1.1328190057465264
1101	18	1.0445566273393325
1101	19	1.0894935870683184
1101	20	1.1435710397605945
1101	21	1.0681353806558533
1101	22	1.1464872345538808
1101	23	1.3991466471430627
1101	24	1.1832127992546049
1101	25	1.1460456423827736
1102	1	0.3264960267792496
1102	2	0.5277712826897023
1102	3	0.37433333778818323
1102	4	0.4195903149685307
1102	5	0.3022685632660824
1102	6	0.3411246237267207
1102	7	0.2850121886445649
1102	8	0.49804309368996513
1102	9	0.6897174578835461
1102	10	0.6979603067073725
1102	11	0.4798972944422104
1102	12	0.537777571773681
1102	13	0.5347074028347265
1102	14	1.1195122329690008
1102	15	0.9157782288522398
1102	16	0.6610290312862475
1102	17	0.937574133868516
1102	18	0.9130997171662827
1102	19	0.8917884503263025
1102	20	0.8406278955247046
1102	21	0.884381827862569
1102	22	0.9083435206057331
1102	23	1.1589730762872748
1102	24	0.9635566590313687
1102	25	0.9814121277390129
1103	1	0.37282166531618444
1103	2	0.5902039393426257
1103	3	0.37401526029695376
1103	4	0.3478957452726732
1103	5	0.44937083374873055
1103	6	0.3399401360706449
1103	7	0.35347795711590635
1103	8	0.5933153873879036
1103	9	0.7732843062497565
1103	10	0.7049549130426627
1103	11	0.524397061669525
1103	12	0.43598202675637054
1103	13	0.6011114288340025
1103	14	1.2571091667213494
1103	15	1.0062160351652238
1103	16	0.8515244304429999
1103	17	0.8014482964097027
1103	18	0.8935590113808942
1103	19	0.9424226106675704
1103	20	1.0054540054166523
1103	21	1.0245090511128794
1103	22	0.8161642763384904
1103	23	1.3064419347095821
1103	24	1.0108196821018112
1103	25	0.9460187690033806
1104	1	0.3125071679382465
1104	2	0.48535960350208995
1104	3	0.3111418253312408
1104	4	0.2836279149443328
1104	5	0.2973381658363081
1104	6	0.18695891267473286
1104	7	0.1790017519921463
1104	8	0.45556397905563983
1104	9	0.4646665151656748
1104	10	0.5642278231723381
1104	11	0.4193166680669407
1104	12	0.42772536362414826
1104	13	0.4005121561100111
1104	14	0.9379382963431908
1104	15	0.8392266966844346
1104	16	0.67228725707065
1104	17	0.6859143795345588
1104	18	0.6256617013018655
1104	19	0.7732760370351741
1104	20	0.8032315182966485
1104	21	0.7701361304259393
1104	22	0.8856354834491427
1104	23	1.2265692385642453
1104	24	1.0872744761942954
1104	25	0.9417474020390344
1105	1	0.32702591086853516
1105	2	0.33959567093871357
1105	3	0.3297757700210049
1105	4	0.4529794978559849
1105	5	0.33534645355680937
1105	6	0.32859509670834797
1105	7	0.2724963951231132
1105	8	0.3661221742170824
1105	9	-1.4145903586125215
1105	10	-0.022474526323826183
1105	11	0.18990573291309687
1105	12	0.3178703339156215
1105	13	0.38047260276391626
1105	14	-1.5007863332539273
1105	15	-0.0985603048985908
1105	16	0.11118254126287042
1105	17	0.24266300485300007
1105	18	0.25035854170101857
1105	19	0.3377738439893676
1105	20	0.5198816935995723
1105	21	0.8057022425531386
1105	22	0.716177183542996
1105	23	1.1386646677249033
1105	24	0.8796201962918744
1105	25	0.5576376134470209
1106	1	0.09591353255034818
1106	2	0.1590224044181241
1106	3	0.22174462067416556
1106	4	-0.8688052884749453
1106	5	-0.09337240008975556
1106	6	0.06473190119127281
1106	7	0.1763677626291269
1106	8	0.2431322440232293
1106	9	0.39601182417661107
1106	10	0.5079182380235494
1106	11	0.4387936838691857
1106	12	0.3618793380704659
1106	13	0.30966740741447296
1106	14	0.8072505035577393
1106	15	0.462789480175104
1106	16	0.33726300990997515
1106	17	0.12021937062284138
1106	18	0.2703806629457972
1106	19	0.1965930065550694
1106	20	0.5499010309799814
1106	21	0.5925510278303889
1106	22	0.7290959605584533
1106	23	0.9149317750204204
1106	24	0.5739444057342595
1106	25	0.6095521937121191
1107	1	0.4043958444577882
1107	2	0.34432053819155684
1107	3	0.25002241165572414
1107	4	0.40948408660874946
1107	5	0.44143594232610894
1107	6	0.15434723583769028
1107	7	0.26827979794589396
1107	8	0.24729575877949372
1107	9	0.49437326678641824
1107	10	0.3718597437395559
1107	11	0.20292412684525374
1107	12	0.2504667139044088
1107	13	0.23474559178992394
1107	14	0.6712686084562733
1107	15	0.4830330589243843
1107	16	0.5117393752377453
1107	17	0.5654476295356119
1107	18	0.6219962405812325
1107	19	0.452609645094725
1107	20	0.6366499377511303
1107	21	0.7905719421785307
1107	22	0.5886353411847765
1107	23	0.9343891313354291
1107	24	0.7730680822644516
1107	25	0.8078078385508647
1108	1	0.5095883970372341
1108	2	0.5002574648116003
1108	3	0.5277946056445941
1108	4	0.7221335023571598
1108	5	0.5372782427949048
1108	6	0.4226933584529533
1108	7	0.4969204910257048
1108	8	0.4913027640464307
1108	9	0.3825996949589836
1108	10	0.520840935923502
1108	11	0.4074154826761358
1108	12	0.43779111448122876
1108	13	0.4310205310958537
1108	14	-0.9209097815384268
1108	15	0.08148305753788776
1108	16	0.3237827007664862
1108	17	0.4706957657270081
1108	18	0.69194190739438
1108	19	0.6270777067327415
1108	20	0.7065293722373625
1108	21	0.7973577919846448
1108	22	0.9202606536235436
1108	23	1.0736635507820225
1108	24	0.9773983672613795
1108	25	0.7698614571501778
1109	1	0.4306180270179141
1109	2	0.3744077306400371
1109	3	0.3863858377600735
1109	4	0.4622396732772245
1109	5	0.4517830193201249
1109	6	0.3237675565985825
1109	7	0.24486744493479762
1109	8	0.386292406079356
1109	9	0.4352420042128813
1109	10	0.4472651698363137
1109	11	0.29663138618141716
1109	12	0.342185180230814
1109	13	0.31890789206019576
1109	14	0.7672325358622416
1109	15	0.5009071964189289
1109	16	0.3762366419921932
1109	17	0.4755956089369917
1109	18	0.5163730628293948
1109	19	0.5558183091117783
1109	20	0.6681498930793026
1109	21	0.5557037776117681
1109	22	0.7404272065250126
1109	23	1.034120201199002
1109	24	0.9767231371484328
1109	25	0.732794557841757
1110	1	0.3957973885317685
1110	2	0.27146433240883283
1110	3	0.3455686624860128
1110	4	0.5524200309211054
1110	5	0.3932565197708726
1110	6	0.21796941570927686
1110	7	0.291727809086737
1110	8	0.18749925912195664
1110	9	0.36070404252736665
1110	10	0.4088619081745364
1110	11	0.10460378839880491
1110	12	0.13288688743167398
1110	13	0.1716447593308459
1110	14	0.5191407474601846
1110	15	0.3757105022424696
1110	16	0.263613184126001
1110	17	0.4163575820049559
1110	18	0.40968089523383355
1110	19	0.2912794177048923
1110	20	0.4887076511890456
1110	21	0.5017409233007726
1110	22	0.6178880627651893
1110	23	0.7109102883769793
1110	24	0.754388656605343
1110	25	0.6306323330849395
1111	1	0.1702142055894491
1111	2	0.10563643190003091
1111	3	0.28602188965714603
1111	4	0.4266154186038804
1111	5	0.33841405130407765
1111	6	0.2818811145816227
1111	7	0.18078578258204914
1111	8	0.26558198966852586
1111	9	0.30909306536887016
1111	10	0.2109118314710443
1111	11	0.15910145794939012
1111	12	0.1156458819556434
1111	13	0.23704186497625557
1111	14	-1.6831736846975716
1111	15	-0.5443591585503853
1111	16	-0.09489845833953581
1111	17	0.1177208011715365
1111	18	0.12985252847212791
1111	19	0.22316365819357742
1111	20	0.2853282550028595
1111	21	0.29160187885838695
1111	22	0.15260902833257717
1111	23	-1.9341394425494443
1111	24	-0.43973973575137393
1111	25	-0.0597916717666962
1112	1	-0.1627617005926929
1112	2	-0.25584661207870596
1112	3	0.013682751754114264
1112	4	0.06925739099082051
1112	5	-0.6140593563914223
1112	6	-0.6749845138300188
1112	7	-0.8550460137751728
1112	8	-0.5656567733217628
1112	9	-0.4881049340783008
1112	10	-0.5861243022882761
1112	11	-0.5967284819910361
1112	12	-0.6748022159611295
1112	13	-0.7439991833276594
1112	14	-0.3996601852487913
1112	15	-0.746456657591672
1112	16	-0.6747198140449697
1112	17	-0.6485987990076225
1112	18	-0.8810963941645014
1112	19	-0.6736475169001395
1112	20	-0.7906729208852283
1112	21	-1.614479019817388
1112	22	-1.4997431532996188
1112	23	-1.2822193620007951
1112	24	-1.4694409085454367
1112	25	-1.7509349340011582
1113	1	-0.017841966519235086
1113	2	-0.13622272767993238
1113	3	-0.14282008862755902
1113	4	0.13178322805752174
1113	5	0.030231334978054747
1113	6	-0.15055761901813014
1113	7	-0.08107012669124458
1113	8	-0.05129258511435353
1113	9	0.20989243890294063
1113	10	-0.12724532382571851
1113	11	-0.32829956556069817
1113	12	-0.18951658290115603
1113	13	-0.24383570198907023
1113	14	-0.19855782957494483
1113	15	-0.2554049160193159
1113	16	-0.25077778209363544
1113	17	-0.18638802590522374
1113	18	-0.1674200445810925
1113	19	-0.28166197829347084
1113	20	-0.8061861681835012
1113	21	-0.5601255443273843
1113	22	-0.15003122230130117
1113	23	-0.262239175101854
1113	24	-0.03906113031109015
1113	25	-1.2545114080365778
1114	1	-0.971859216466612
1114	2	-0.8053526655566685
1114	3	-0.5622554522692553
1114	4	-1.6626865230168733
1114	5	-0.9642297651698755
1114	6	-0.762921330880765
1114	7	-0.6541240286916263
1114	8	-0.5974832457663837
1114	9	-0.539534437218677
1114	10	-0.6494765707798678
1114	11	-0.5632952545156726
1114	12	-0.6947051202313475
1114	13	-0.8027739833960155
1114	14	-0.6651332449876293
1114	15	-1.549957043137951
1114	16	-0.9664293266204353
1114	17	-0.9551428019375408
1114	18	-1.0403456245055551
1114	19	-1.0566490739948193
1114	20	-2.1002484606213443
1114	21	-6.751525754448775
1114	22	-7.800645870626724
1114	23	-7.882563085013032
1114	24	-5.779932240157667
1114	25	-7.3854297231052435
1115	1	-1.062120029788538
1115	2	-1.0669484684359531
1115	3	-0.8123076898794359
1115	4	-0.7254232975723056
1115	5	-0.8133605988657351
1115	6	-1.0981822154402323
1115	7	-1.145348637143755
1115	8	-0.8697206490663021
1115	9	-2.7872288488580743
1115	10	-1.7451133006996216
1115	11	-1.2301481536786412
1115	12	-1.0534833710394054
1115	13	-1.5871130444227788
1115	14	-1.2462864851344548
1115	15	-2.1569141989293783
1115	16	-1.4953668157762223
1115	17	-3.149941446589665
1115	18	-3.0594340845509578
1115	19	-2.2644112725453596
1115	20	-1.9398724200897206
1115	21	-1.580994753725271
1115	22	-0.9081136532050635
1115	23	-0.40940978163069275
1115	24	-1.4749791232420293
1115	25	-1.2478734283707524
1116	1	-1.6810804027796422
1116	2	-0.9163296527173763
1116	3	-0.7918767491179217
1116	4	-0.508748928210629
1116	5	-0.37956935191049723
1116	6	-0.5847151945716647
1116	7	-0.8952229648059031
1116	8	-1.9699522426159604
1116	9	-1.0294623716215483
1116	10	-2.6561027393403904
1116	11	-1.3153255561443373
1116	12	-0.8694397418029567
1116	13	-0.9143751648147997
1116	14	-1.1975306229685074
1116	15	-1.4987579726493436
1116	16	-1.0116529497777265
1116	17	-0.9108638315002864
1116	18	-1.4505975674777076
1116	19	-1.0649817846757017
1116	20	-1.40406514225144
1116	21	-0.8272104843619061
1116	22	-0.33473638887518575
1116	23	-0.21179402819115012
1116	24	-0.284911522632747
1116	25	-0.22953051610749498
1201	1	0.3207150975508597
1201	2	0.2916369962816212
1201	3	0.28421116905590793
1201	4	0.4024873355770353
1201	5	0.2962375941833315
1201	6	0.2103932932067707
1201	7	0.27650518187692086
1201	8	0.31064363095408254
1201	9	0.6115412696290647
1201	10	0.71102542503683
1201	11	0.5035730771371192
1201	12	0.4959303424218504
1201	13	0.47654180863050044
1201	14	1.1486232643064156
1201	15	0.8376851111261487
1201	16	0.8893843108300246
1201	17	0.7630785205770749
1201	18	0.7007206785743918
1201	19	0.7173343479381984
1201	20	0.8690732907572212
1201	21	0.8574761363474366
1201	22	0.8943850895853558
1201	23	0.6519040422908233
1201	24	0.7693891650699385
1201	25	0.7245126940412803
1202	1	-0.05192248117507248
1202	2	0.10997496507216198
1202	3	0.07715283663394246
1202	4	0.14073932860920024
1202	5	0.21483367609618398
1202	6	0.12530502652466424
1202	7	0.10329662347194812
1202	8	0.27747105541972417
1202	9	0.29607585471454456
1202	10	0.5501935731198557
1202	11	0.1946206046907477
1202	12	0.08417922608795436
1202	13	0.35591027136707254
1202	14	0.5795026287662921
1202	15	0.5525106399506896
1202	16	0.46360259118619496
1202	17	0.3996074959373175
1202	18	0.33680676584107516
1202	19	0.38226548798905213
1202	20	0.5468913592476241
1202	21	0.48692404874766027
1202	22	0.46695919435598654
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2211	5	0.25833138091552144
2211	6	0.1295479394153034
2211	7	0.11669359834858994
2211	8	0.2406744181012641
2211	9	0.4142760460691832
2211	10	0.38404141392999236
2211	11	0.13832148943720313
2211	12	0.22886289966704254
2211	13	0.10519462651268441
2211	14	0.48145057600401486
2211	15	0.23055796259826167
2211	16	0.1100512425659872
2211	17	0.16731306779743704
2211	18	0.22825772852691983
2211	19	0.2411622962366522
2211	20	0.3012541375449942
2211	21	0.32623259197983145
2211	22	0.3022561703076505
2211	23	0.42875219451881463
2211	24	0.4203606440479888
2211	25	0.4983852913075921
2212	1	-0.12338907655102815
2212	2	-0.16614475642503734
2212	3	-0.08026761207543842
2212	4	-0.4803983679972248
2212	5	-0.15394755756148015
2212	6	-0.5051021044227753
2212	7	-0.5019129923320804
2212	8	-0.24901016158780465
2212	9	-0.2138701378796668
2212	10	-0.46872637256787897
2212	11	-0.42664654084315146
2212	12	-0.5211202066349472
2212	13	-0.5601442097467526
2212	14	-0.42960945008498896
2212	15	-0.6505963688176024
2212	16	-0.39323768243882284
2212	17	-0.3884344248783975
2212	18	-0.5024071133352379
2212	19	-0.4465399469898301
2212	20	-0.41576872240838725
2212	21	-0.3257520967698184
2212	22	-0.31191272390358193
2212	23	0.0022718575002329544
2212	24	-0.21523416381293003
2212	25	-0.9118069546988679
2213	1	-0.23743885742292647
2213	2	-1.2743877474711915
2213	3	-0.4553666915277219
2213	4	-0.09784557804566418
2213	5	-0.12209157400132398
2213	6	-0.08104708125181048
2213	7	-0.04909703896757378
2213	8	0.16868364620854237
2213	9	0.10495181267496889
2213	10	-0.48174153329676983
2213	11	-0.1015304763418996
2213	12	-0.02795532584482885
2213	13	-0.30514662120431524
2213	14	-0.038151461869681214
2213	15	-0.15436369461600208
2213	16	-0.20826462785600341
2213	17	0.05773890649781066
2213	18	-0.07511822071848684
2213	19	0.023032910482541524
2213	20	0.006516081215089287
2213	21	-0.5078825671088936
2213	22	-0.8979841449860118
2213	23	-0.2639614457772552
2213	24	-0.050799598189826156
2213	25	-0.7305102196027207
2214	1	-0.2764894331736585
2214	2	-0.5130176112478146
2214	3	-0.569401086048682
2214	4	-0.7017915402643737
2214	5	-0.3702337430387317
2214	6	-0.277568493158185
2214	7	-0.5774156963018058
2214	8	-0.22688909406390678
2214	9	-0.1965421287715543
2214	10	-2.0261447093334937
2214	11	-0.9556438954733864
2214	12	-0.5148726134191506
2214	13	-0.4100152898277827
2214	14	-0.9073954597623555
2214	15	-0.5915895544790288
2214	16	-0.7736812004457505
2214	17	-0.48397329339682216
2214	18	-0.6008362157512295
2214	19	-1.6319196747511455
2214	20	-2.5961666161582784
2214	21	-1.984806628221257
2214	22	-2.1032596021998344
2214	23	-2.6335971799535116
2214	24	-1.7224926063933381
2214	25	-2.1863226678604164
2215	1	-0.18644898827982814
2215	2	-0.23733405041195255
2215	3	-0.4794512208649948
2215	4	-0.16955990898698303
2215	5	-0.19393086976606355
2215	6	-0.15158475537591443
2215	7	-0.21704958575243438
2215	8	-0.25817279814209826
2215	9	-0.26823175634149266
2215	10	-0.38589993346561613
2215	11	-0.4891362699256945
2215	12	-0.45549129390449394
2215	13	-0.3295626019275488
2215	14	-0.12484785275190546
2215	15	-0.5839671665450865
2215	16	-0.4796563015579878
2215	17	-0.3320605869593436
2215	18	-0.3454224981914038
2215	19	-2.0560517947376624
2215	20	-2.2372666330103215
2215	21	-3.2383339706546934
2215	22	-2.4704823861566894
2215	23	-3.2337720198591455
2215	24	-2.069935309460405
2215	25	-1.4133838828924397
2216	1	-1.5201025307579847
2216	2	-1.1830229606481169
2216	3	-0.9242732635361541
2216	4	-0.9897328657894562
2216	5	-1.9091327989165023
2216	6	-1.5405346887022304
2216	7	-1.136342625370638
2216	8	-5.370740568433707
2216	9	-4.1105950972194805
2216	10	-2.029212168086808
2216	11	-1.0680014794214259
2216	12	-1.3436598949853078
2216	13	-2.009227895642674
2216	14	-3.691904253425662
2216	15	-2.222511903710405
2216	16	-3.1427156806154315
2216	17	-2.5463857790848294
2216	18	-2.7128756511548744
2216	19	-1.7155115624325106
2216	20	-1.0376831242199245
2216	21	-0.8028369227198198
2216	22	-0.5824283804718817
2216	23	-0.986113275078992
2216	24	-0.5027856972205242
2216	25	-0.43251802420555663
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	37.0
8	213.0
9	570.0
10	1195.0
11	2438.0
12	4147.0
13	6467.0
14	9724.0
15	14233.0
16	17973.0
17	20061.0
18	20422.0
19	20731.0
20	21079.0
21	22449.0
22	23383.0
23	23936.0
24	25265.0
25	26825.0
26	27774.0
27	30879.0
28	34466.0
29	40479.0
30	48402.0
31	58982.0
32	79117.0
33	113308.0
34	164914.0
35	262453.0
36	458380.0
37	1504503.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.728059540134694	19.217789924002147	11.256709436062028	23.797441099801127
2	12.54286887005148	22.804228755877144	36.956321573748724	27.696580800322657
3	21.245604262706056	29.03871231727253	26.968087417378296	22.747596002643117
4	13.006453830229086	19.6487598951435	33.82899700572107	33.515789268906346
5	11.590875637175722	40.591165490932504	34.545023625999164	13.272935245892603
6	32.114657437256696	33.539786917856	18.23598406552224	16.10957157936506
7	24.359205849316247	34.08704926243312	22.364071634997998	19.189673253252636
8	26.225021897677713	36.576966690244625	21.397580784763495	15.800430627314169
9	26.333100147108308	18.682430833486233	20.36850339178981	34.615965627615644
10	16.20559484311002	32.42285979178587	31.70430545852979	19.66723990657432
11	28.654073884613602	28.282508040273825	19.838737441174956	23.224680633937623
12	23.180753418573726	31.87165574309364	28.111889200023597	16.835701638309033
13	28.151437712316195	23.135564346536988	22.743600973441087	25.969396967705734
14	21.00356231589109	27.132502655771496	25.964384621194757	25.89955040714266
15	30.294555569999375	25.847613533548436	21.39603578767673	22.461795108775462
16	18.846122675242242	29.920576517231655	30.75598188496238	20.477318922563725
17	25.150167064810514	30.271683333165843	27.17287179984251	17.40527780218113
18	25.08131939379204	26.837604343950076	28.47540319312748	19.6056730691304
19	22.704213864593186	31.540398334669455	28.911923749826972	16.843464050910384
20	23.223286077912444	28.403693469519542	27.401909234840815	20.971111217727202
21	19.172604604550045	30.268445728334598	30.260821549109934	20.298128118005422
22	21.527848746426017	29.80829218773876	22.98855385198357	25.67530521385165
23	17.9950400932286	31.146514504028787	27.28789001517041	23.570555387572202
24	20.571458395331394	35.128635031285924	24.706607356000795	19.59329921738189
25	21.808954777755034	27.610326487779048	22.646861019328053	27.933857715137872
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	10779.0
1	10779.0
2	12772.0
3	14765.0
4	14765.0
5	14765.0
6	17217.0
7	19669.0
8	19669.0
9	19669.0
10	20493.0
11	21317.0
12	21317.0
13	21317.0
14	25567.5
15	29818.0
16	29818.0
17	29818.0
18	40011.0
19	50204.0
20	50204.0
21	50204.0
22	66151.5
23	82099.0
24	82099.0
25	82099.0
26	103371.5
27	124644.0
28	124644.0
29	124644.0
30	145710.0
31	166776.0
32	166776.0
33	166776.0
34	184536.5
35	202297.0
36	202297.0
37	202297.0
38	219101.0
39	235905.0
40	235905.0
41	235905.0
42	271796.0
43	307687.0
44	307687.0
45	307687.0
46	523071.0
47	738455.0
48	738455.0
49	738455.0
50	633523.5
51	528592.0
52	528592.0
53	528592.0
54	384528.5
55	240465.0
56	240465.0
57	240465.0
58	187296.5
59	134128.0
60	134128.0
61	134128.0
62	111381.0
63	88634.0
64	88634.0
65	88634.0
66	69648.0
67	50662.0
68	50662.0
69	50662.0
70	37078.0
71	23494.0
72	23494.0
73	23494.0
74	16537.0
75	9580.0
76	9580.0
77	9580.0
78	6429.0
79	3278.0
80	3278.0
81	3278.0
82	2186.0
83	1094.0
84	1094.0
85	1094.0
86	713.5
87	333.0
88	333.0
89	333.0
90	219.5
91	106.0
92	106.0
93	106.0
94	59.5
95	13.0
96	13.0
97	13.0
98	12.0
99	11.0
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.029240097834384995
2	0.012934367002128173
3	0.018380416266182142
4	0.019126006343999056
5	0.015916727313395822
6	0.005608134063579384
7	0.0
8	9.725087971524941E-5
9	9.725087971524941E-5
10	0.0
11	7.131731179118291E-4
12	0.0012642614362982426
13	0.002528522872596485
14	4.5383743867116397E-4
15	0.0018153497546846559
16	0.0024312719928812356
17	0.0023988550329761523
18	0.0017829327947795728
19	3.8900351886099764E-4
20	2.9175263914574826E-4
21	0.08123690152213836
22	0.07968088744669435
23	0.08068581320375194
24	0.0010049257570575775
25	0.22351493854554827
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3084805.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.859892042039135
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.043621943731	50.85686892893039
2	6.420319876590453	7.172767503634013
3	1.1338797168463042	1.900151957150774
4	0.4071747301368198	0.909789458707567
5	0.22287622951759462	0.6224921059794786
6	0.14315313988551923	0.4797911363690418
7	0.09357178803027837	0.36588369842863433
8	0.07380525532409762	0.3298202875631382
9	0.054703955579107635	0.27501813476293113
>10	0.34312179088312034	3.644575729101186
>50	0.032950203729250324	1.2673876890450626
>100	0.02229904218492024	2.458574693630867
>500	0.0030562829875841356	1.1721819304040695
>1k	0.003291381678935708	3.7044402871276936
>5k	9.403947654102022E-4	4.0321640086438295
>10k+	0.0012342681296008903	20.80809245052135
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	114729	3.7191653929502833	No Hit
CCCATGTACTCTGCGTTGATACCAC	107529	3.485763281633685	No Hit
GAGTACATGGGAAGCAGTGGTATCA	53919	1.747890061122178	No Hit
CATGTACTCTGCGTTGATACCACTG	41114	1.3327908895375882	No Hit
GCGTTGATACCACTGCTTCCCATGT	37699	1.2220869714617293	No Hit
GCTTCCCATGTACTCTGCGTTGATA	35452	1.1492460625550074	No Hit
GTATCAACGCAGAGTACATGGGAAG	30369	0.98447065535747	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	28569	0.9261201275283203	No Hit
ACTCTGCGTTGATACCACTGCTTCC	22399	0.7261074849139573	No Hit
TATCAACGCAGAGTACATGGGAAGC	22311	0.72325479244231	No Hit
GGTATCAACGCAGAGTACATGGGAA	19130	0.6201364429842404	No Hit
CAGTGGTATCAACGCAGAGTACATG	15396	0.49909151469866003	No Hit
GTGGTATCAACGCAGAGTACATGGG	14602	0.47335244853402403	No Hit
GCAGTGGTATCAACGCAGAGTACAT	13764	0.44618703613356436	No Hit
ATACCACTGCTTCCCATGTACTCTG	11695	0.3791163460899473	No Hit
CATGGGAAGCAGTGGTATCAACGCA	11105	0.35999033974594824	No Hit
ACATGGGAAGCAGTGGTATCAACGC	11036	0.35775356951249754	No Hit
GTACTCTGCGTTGATACCACTGCTT	11026	0.3574293999134467	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	10733	0.34793123066125736	No Hit
GTACATGGTAAGCAGTGGTATCAAC	10672	0.3459537961070473	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	10536	0.341545089559956	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	9718	0.315028016357598	No Hit
GATACCACTGCTTCCCATGTACTCT	8850	0.2868900951599858	No Hit
TACCACTGCTTCCCATGTACTCTGC	8827	0.28614450508216893	No Hit
CTTCCCATGTACTCTGCGTTGATAC	8759	0.28394015180862325	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	8519	0.2761600814314033	No Hit
CCATGTACTCTGCGTTGATACCACT	8234	0.2669212478584546	No Hit
GTTGATACCACTGCTTCCCATGTAC	8187	0.26539765074291566	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	8088	0.26218837171231246	No Hit
CCACTGCTTCCCATGTACTCTGCGT	7969	0.25833075348360757	No Hit
GTACATGGGTAAGCAGTGGTATCAA	7482	0.24254369400983208	No Hit
ATGGGAAGCAGTGGTATCAACGCAG	7457	0.24173327001220496	No Hit
ACCATGTACTCTGCGTTGATACCAC	7220	0.2340504505147003	No Hit
TTCCCATGTACTCTGCGTTGATACC	6592	0.21369259969430807	No Hit
CCCCATGTACTCTGCGTTGATACCA	5968	0.19346441671353617	No Hit
TGATACCACTGCTTCCCATGTACTC	5822	0.18873154056739405	No Hit
GGTAAGCAGTGGTATCAACGCAGAG	5122	0.16603966863383585	No Hit
ATCAACGCAGAGTACATGGGAAGCA	4693	0.15213279283455516	No Hit
GCTTACCATGTACTCTGCGTTGATA	4456	0.14444997333705048	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	3937	0.12762557114631232	No Hit
AAAAAGTACTCTGCGTTGATACCAC	3623	0.11744664573611623	No Hit
GAGTACATGGTAAGCAGTGGTATCA	3573	0.11582579774086206	No Hit
GGAAGCAGTGGTATCAACGCAGAGT	3472	0.11255168479044866	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	1.620847995254157E-4	0.0
11	0.0	0.0	0.0	1.620847995254157E-4	0.0
12	0.0	0.0	0.0	2.2691871933558198E-4	0.0
13	0.0	0.0	0.0	2.2691871933558198E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGACGGC	35	0.0021768112	16.279032	6
TCCAACG	40	0.0052842507	14.248542	2
TGATACG	85	3.9207007E-6	12.301435	17
CGCAAAA	310	0.0	12.256809	2
GTACAAA	895	0.0	12.102655	1
CGCTCAT	55	0.003078699	12.0861435	10
GGTATCG	95	1.0334115E-6	12.007142	17
ACGCAAA	370	0.0	11.299255	1
GAAGCGC	60	0.0058951536	11.078965	10
GATACGA	95	1.3523391E-5	11.006547	18
TGGACGG	90	9.5498384E-5	10.552421	5
AAGCGGT	135	5.727088E-8	10.551395	10
ATACGAC	100	2.3491357E-5	10.47049	19
GTGTTAC	155	3.6561687E-9	10.421161	1
GTATCGA	110	6.007489E-6	10.369805	18
GTCTAGG	185	6.002665E-11	10.27205	1
CCAGGAC	355	0.0	10.167479	3
TGCGTAG	75	0.002657155	10.129503	12
GTCCTAG	220	1.8189894E-12	9.933539	1
CTTACAC	300	0.0	9.815184	3
>>END_MODULE