FastQCFastQC Report
Wed 25 May 2016
SRR1294698_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294698_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3084805
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC1207823.915385251255752No Hit
GTACATGGGAAGCAGTGGTATCAAC1134323.677120595953391No Hit
GAGTACATGGGAAGCAGTGGTATCA598031.938631453203687No Hit
CATGTACTCTGCGTTGATACCACTG483031.565836414295231No Hit
GCGTTGATACCACTGCTTCCCATGT385011.248085373305606No Hit
GCTTCCCATGTACTCTGCGTTGATA375391.2169002578769161No Hit
ACGCAGAGTACATGGGAAGCAGTGG360021.1670753905028033No Hit
GTATCAACGCAGAGTACATGGGAAG326051.0569549777052358No Hit
ACTCTGCGTTGATACCACTGCTTCC268850.8715299670481602No Hit
TATCAACGCAGAGTACATGGGAAGC258480.8379135796265891No Hit
GGTATCAACGCAGAGTACATGGGAA214520.6954086238838435No Hit
CAGTGGTATCAACGCAGAGTACATG189210.613361298364078No Hit
GTGGTATCAACGCAGAGTACATGGG161380.5231448989482318No Hit
GCAGTGGTATCAACGCAGAGTACAT145510.47169918357886476No Hit
CATGGGAAGCAGTGGTATCAACGCA145340.4711480952604784No Hit
ACATGGGAAGCAGTGGTATCAACGC136520.442556336624195No Hit
ATACCACTGCTTCCCATGTACTCTG134230.43513285280593106No Hit
GGGAAGCAGTGGTATCAACGCAGAG126770.410949800716739No Hit
CTGCTTCCCATGTACTCTGCGTTGA119290.3867019147077368No Hit
GTACTCTGCGTTGATACCACTGCTT111270.36070351286386015No Hit
CTTCCCATGTACTCTGCGTTGATAC106990.3468290540244845No Hit
AAGCAGTGGTATCAACGCAGAGTAC105750.34280935099625426No Hit
TACCACTGCTTCCCATGTACTCTGC104430.3385303122887832No Hit
GTACATGGTAAGCAGTGGTATCAAC103990.3371039660529596No Hit
GATACCACTGCTTCCCATGTACTCT103160.3344133583808377No Hit
CCATGTACTCTGCGTTGATACCACT101310.32841622079839733No Hit
GCAGAGTACATGGGAAGCAGTGGTA98140.318140044508486No Hit
CCACTGCTTCCCATGTACTCTGCGT95180.30854462437658137No Hit
GTATCAACGCAGAGTACTTTTTTTT92260.29907887208429706No Hit
ATGGGAAGCAGTGGTATCAACGCAG91610.2969717696904667No Hit
GTTGATACCACTGCTTCCCATGTAC91170.295545423454643No Hit
GTACATGGGTAAGCAGTGGTATCAA83370.2702601947286781No Hit
ACCATGTACTCTGCGTTGATACCAC82200.2664674104197834No Hit
CCCCATGTACTCTGCGTTGATACCA70050.2270808041351074No Hit
TTCCCATGTACTCTGCGTTGATACC69010.22370944030497877No Hit
GGTATCAACGCAGAGTACTTTTTTT67320.21823097408101969No Hit
TATCAACGCAGAGTACTTTTTTTTT67290.21813372320130445No Hit
GGTAAGCAGTGGTATCAACGCAGAG62790.20354609124401704No Hit
TGATACCACTGCTTCCCATGTACTC62040.20111481925113578No Hit
ATCAACGCAGAGTACATGGGAAGCA56750.18396624746134682No Hit
ACGCAGAGTACTTTTTTTTTTTTTT50270.16296005744285294No Hit
GTACTTTTTTTTTTTTTTTTTTTTT47310.15336463731094835No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA47120.15274871507275176No Hit
GCTTACCATGTACTCTGCGTTGATA46620.15112786707749762No Hit
GGAAGCAGTGGTATCAACGCAGAGT43070.1396198463111931No Hit
GAGTACATGGTAAGCAGTGGTATCA40540.13141835545520705No Hit
AAAAAGTACTCTGCGTTGATACCAC40020.12973267354014273No Hit
GAGTACTTTTTTTTTTTTTTTTTTT35230.11420494974560792No Hit
TGGGAAGCAGTGGTATCAACGCAGA33890.10986107711832677No Hit
CTGCGTTGATACCACTGCTTCCCAT31030.10058982658547298No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCAG604.0452598E-412.6846845
TCGATTC600.005902346711.0771513
CGCAAAA1303.201785E-810.9773082
CTACGCA700.001474535610.8725854
CTAGGAC2550.010.4462093
CTGGACG1303.7708924E-710.2453214
TATTCCG750.002617786210.1477465
CACACCG856.511222E-410.0731315
GTCCTAG2700.09.87019351
GAACAAA6500.09.8105381
TACACTG5500.09.6864855
CTTACAC3150.09.664523
TAGGACT2700.09.5135134
ACACCGT800.0045366019.4951626
GTACTTG4050.09.4001841
AAAAGTA8250.09.3406922
AAAAAGT7750.09.3334731
AGACTGT3800.09.2452896
GTTCTAG2304.0017767E-119.1038731
CTGATCA2201.70985E-109.0631239
Produced by FastQC (version 0.11.5)