FastQCFastQC Report
Wed 25 May 2016
SRR1294697_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294697_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3790512
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC1582254.174238203176774No Hit
CCCATGTACTCTGCGTTGATACCAC1484243.9156715504396242No Hit
GAGTACATGGGAAGCAGTGGTATCA744101.9630593439619766No Hit
CATGTACTCTGCGTTGATACCACTG577441.5233825931694716No Hit
GCGTTGATACCACTGCTTCCCATGT490811.2948382698696113No Hit
GCTTCCCATGTACTCTGCGTTGATA433441.1434866846484064No Hit
GTATCAACGCAGAGTACATGGGAAG416011.0975034507211692No Hit
ACGCAGAGTACATGGGAAGCAGTGG403391.0642097954049479No Hit
ACTCTGCGTTGATACCACTGCTTCC321490.8481439974335921No Hit
TATCAACGCAGAGTACATGGGAAGC314020.8284368971790619No Hit
GGTATCAACGCAGAGTACATGGGAA268160.7074506029792281No Hit
CAGTGGTATCAACGCAGAGTACATG219640.5794467871358803No Hit
GTGGTATCAACGCAGAGTACATGGG207090.5463378034418569No Hit
GCAGTGGTATCAACGCAGAGTACAT179190.4727329711659006No Hit
ATACCACTGCTTCCCATGTACTCTG165680.4370913480817367No Hit
CATGGGAAGCAGTGGTATCAACGCA162110.427673095349652No Hit
GTACTCTGCGTTGATACCACTGCTT157350.41511542504020565No Hit
ACATGGGAAGCAGTGGTATCAACGC153210.40419341767022504No Hit
CTGCTTCCCATGTACTCTGCGTTGA148150.39084429755135985No Hit
GGGAAGCAGTGGTATCAACGCAGAG147130.3881533681993356No Hit
GTACATGGTAAGCAGTGGTATCAAC143960.37979038187980935No Hit
TACCACTGCTTCCCATGTACTCTGC127820.3372103821330733No Hit
GATACCACTGCTTCCCATGTACTCT125750.331749378448083No Hit
CTTCCCATGTACTCTGCGTTGATAC124660.32887377747386104No Hit
GCAGAGTACATGGGAAGCAGTGGTA124490.3284252892485237No Hit
AAGCAGTGGTATCAACGCAGAGTAC123430.3256288332552436No Hit
GTTGATACCACTGCTTCCCATGTAC120810.31871683825298536No Hit
CCACTGCTTCCCATGTACTCTGCGT115280.30412778009936386No Hit
CCATGTACTCTGCGTTGATACCACT111330.29370702427534856No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA106210.28019961419459954No Hit
ATGGGAAGCAGTGGTATCAACGCAG102370.2700690566340378No Hit
GTACATGGGTAAGCAGTGGTATCAA97850.2581445461721266No Hit
ACCATGTACTCTGCGTTGATACCAC96370.2542400604456601No Hit
TTCCCATGTACTCTGCGTTGATACC92300.24350272469787723No Hit
TGATACCACTGCTTCCCATGTACTC83980.22155318331666013No Hit
CCCCATGTACTCTGCGTTGATACCA75930.20031594676392003No Hit
GGTAAGCAGTGGTATCAACGCAGAG70930.1871251166069386No Hit
ATCAACGCAGAGTACATGGGAAGCA67040.17686265074480703No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA56860.15000612054519286No Hit
GCTTACCATGTACTCTGCGTTGATA53160.14024490622902658No Hit
AAAAAGTACTCTGCGTTGATACCAC49190.12977138708438332No Hit
GAGTACATGGTAAGCAGTGGTATCA47400.12504906988818398No Hit
GGAAGCAGTGGTATCAACGCAGAGT47140.12436314672002094No Hit
TCCATGTACTCTGCGTTGATACCAC39160.10331058178947856No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAAA9200.012.5980961
CGCAAAA3350.012.47573952
GTCTGCG1302.0008883E-1012.4177719
TCAGGCG600.00588544611.0815253
CCGTACT600.005889493511.0805014
AAGCGGT1701.4006218E-1010.61311310
GAACAAA17950.010.532261
CTAGTAC1606.199116E-910.0921033
ACGCAAA5100.010.0590411
GAAGCGG1856.548362E-109.752599
GTCTTAC2450.09.6940871
GTCTTAG3000.09.5002051
ATCGGTC800.00453589869.49544214
GTACAAG5150.09.4079691
GTATTAG4250.09.3884371
CCTACAC2357.2759576E-129.2963553
CGGTGGT2252.7284841E-119.284554513
TAGGACC1856.7375368E-99.2408814
GTCCTAG2800.09.1609121
CGGCTTC1356.894197E-69.1438813
Produced by FastQC (version 0.11.5)