##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294697_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3790512
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.85767516367182	33.0	33.0	33.0	27.0	33.0
2	30.88952257636963	33.0	33.0	33.0	27.0	33.0
3	30.891721223940195	33.0	33.0	33.0	27.0	33.0
4	34.141981610927495	37.0	37.0	37.0	27.0	37.0
5	34.686986349073685	37.0	37.0	37.0	27.0	37.0
6	34.719598566104	37.0	37.0	37.0	27.0	37.0
7	34.780842271439845	37.0	37.0	37.0	27.0	37.0
8	34.66080809136074	37.0	37.0	37.0	27.0	37.0
9	34.34139266674264	37.0	37.0	37.0	27.0	37.0
10	34.490168082834195	37.0	37.0	37.0	27.0	37.0
11	34.74861812863276	37.0	37.0	37.0	27.0	37.0
12	34.6953398379955	37.0	37.0	37.0	27.0	37.0
13	34.52413710865445	37.0	37.0	37.0	27.0	37.0
14	35.798459152747704	40.0	37.0	40.0	27.0	40.0
15	35.990031953466975	40.0	37.0	40.0	27.0	40.0
16	35.976554354662376	40.0	37.0	40.0	27.0	40.0
17	35.99107904156483	40.0	37.0	40.0	27.0	40.0
18	35.966907109118765	40.0	37.0	40.0	27.0	40.0
19	35.936675309298586	40.0	37.0	40.0	27.0	40.0
20	35.881962911606664	40.0	37.0	40.0	27.0	40.0
21	35.7909045005002	40.0	37.0	40.0	27.0	40.0
22	35.73759877293622	40.0	37.0	40.0	27.0	40.0
23	35.40878488183127	37.0	37.0	40.0	27.0	40.0
24	35.75706342573246	37.0	37.0	40.0	27.0	40.0
25	34.67570001097477	37.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.4697652313773162
1101	2	0.6234386336810509
1101	3	0.4194664309237659
1101	4	0.5516579614962467
1101	5	0.5809583717421276
1101	6	0.32873632724263757
1101	7	0.369987071951563
1101	8	0.4818540345120752
1101	9	0.7259556309953936
1101	10	0.7329668450603677
1101	11	0.5805222129676793
1101	12	0.5540933363149065
1101	13	0.545617255166583
1101	14	1.2180396530188915
1101	15	0.9917355795265124
1101	16	0.8757224269214063
1101	17	1.0217558432059732
1101	18	0.9269334538846863
1101	19	0.930258711907193
1101	20	0.9664936435607032
1101	21	0.9877320185387575
1101	22	1.0476000548175435
1101	23	1.3476410292164829
1101	24	1.1095134334634906
1101	25	1.0057626444239745
1102	1	0.3264896270579847
1102	2	0.4841923143228115
1102	3	0.27222440954067295
1102	4	0.3178880232963266
1102	5	0.3401871388105633
1102	6	0.23831684086400884
1102	7	0.3091296125620886
1102	8	0.3117528252060211
1102	9	0.5730131864886587
1102	10	0.6250863674779126
1102	11	0.4520208314756644
1102	12	0.4052182512230047
1102	13	0.5326743101192619
1102	14	1.1098027238020052
1102	15	0.8689522401866938
1102	16	0.6209804389076794
1102	17	0.7598557577803859
1102	18	0.7374021267180098
1102	19	0.7931728925556314
1102	20	0.8757841105984383
1102	21	0.8153331277366931
1102	22	0.8543957942160958
1102	23	1.2244883910339155
1102	24	0.9677038791448282
1102	25	0.9037830064149261
1103	1	0.2833400911718762
1103	2	0.4435261170000224
1103	3	0.2438088353450496
1103	4	0.08496092714207037
1103	5	0.3264978195271979
1103	6	0.20821390497172843
1103	7	0.22844396944445577
1103	8	0.3794058185739715
1103	9	0.56079979653056
1103	10	0.6544906855499804
1103	11	0.4187963902577394
1103	12	0.3758133478260035
1103	13	0.3724292866005854
1103	14	0.9196130622661443
1103	15	0.6927888825027537
1103	16	0.40117073454554486
1103	17	0.5041103220954
1103	18	0.5313211209997135
1103	19	0.5233794634783138
1103	20	0.6479593243639741
1103	21	0.7540651838693506
1103	22	0.6153462318277718
1103	23	1.004188379490003
1103	24	0.7409840563031764
1103	25	0.556135443561594
1104	1	0.4054636975167085
1104	2	0.5178007633429011
1104	3	0.3412761387911907
1104	4	0.43771324693601343
1104	5	0.40456440185507603
1104	6	0.31753586109697096
1104	7	0.4031627245398184
1104	8	0.5089973406039405
1104	9	0.6179854649447165
1104	10	0.6207033661779917
1104	11	0.4234437425022506
1104	12	0.3473409248653354
1104	13	0.49749386763244985
1104	14	0.9381937522480399
1104	15	0.8218757210316596
1104	16	0.7036689878824092
1104	17	0.77705346953082
1104	18	0.695660197542189
1104	19	0.8118561071968386
1104	20	0.8994019363766697
1104	21	0.8902918619865403
1104	22	0.9212744237027124
1104	23	1.3390397091496524
1104	24	1.0385045901755845
1104	25	0.906998032309204
1105	1	0.1873865399624961
1105	2	0.2952704702258053
1105	3	0.22532096680999558
1105	4	0.11223417434786143
1105	5	0.19211268697501538
1105	6	0.16332332837166774
1105	7	0.19051532822800255
1105	8	0.23392109797997307
1105	9	-1.6113064795623941
1105	10	-0.1973027759874313
1105	11	0.04476501128303312
1105	12	0.19361314202128455
1105	13	0.23214028244295548
1105	14	-1.6649763056987936
1105	15	-0.3217133820910476
1105	16	-0.11930213696421532
1105	17	0.17206236473802505
1105	18	0.14077872873317432
1105	19	0.22228464296903638
1105	20	0.3762109207328379
1105	21	0.5352715503101848
1105	22	0.4821547894947855
1105	23	0.7552507390565637
1105	24	0.651117050741604
1105	25	0.36696566189353774
1106	1	-0.02483317466729318
1106	2	0.10211708878009418
1106	3	0.04050192952097831
1106	4	-1.213042685714882
1106	5	-0.35810074919868384
1106	6	-0.20627562663867138
1106	7	-0.03639392478807224
1106	8	0.05490395225807987
1106	9	0.2172968102150108
1106	10	0.17154108693572567
1106	11	0.11608373566240715
1106	12	0.005354021885089821
1106	13	0.09329546170016556
1106	14	0.48193099227702163
1106	15	0.2797051290819965
1106	16	0.14228515872633096
1106	17	0.04860316677547161
1106	18	0.17662304110920246
1106	19	0.06213082145163895
1106	20	0.18301307244040288
1106	21	0.4371868799929217
1106	22	0.5218539271850631
1106	23	0.6649892317824353
1106	24	0.22182463741419411
1106	25	0.19829099813653528
1107	1	0.2756479318539249
1107	2	0.29733402848024326
1107	3	0.2740582860199119
1107	4	0.4444199063595562
1107	5	0.31531689578420696
1107	6	0.18739705041834753
1107	7	0.1579797686973663
1107	8	0.23057352081307414
1107	9	0.4146734648271675
1107	10	0.38514988886669954
1107	11	0.1759262192008748
1107	12	0.2803920241763649
1107	13	0.25030068584865006
1107	14	0.7154155708740717
1107	15	0.5108293965924702
1107	16	0.4631813851040363
1107	17	0.498579662395251
1107	18	0.5437950429422784
1107	19	0.5142117212006099
1107	20	0.6209718501607426
1107	21	0.6346060176102597
1107	22	0.578524022039268
1107	23	0.7465588718054477
1107	24	0.6980422979922096
1107	25	0.5459974959896527
1108	1	0.47173300187580836
1108	2	0.4744980684833422
1108	3	0.5881202684679785
1108	4	0.73587326590809
1108	5	0.6500013361200487
1108	6	0.5037964559815578
1108	7	0.5183263497600166
1108	8	0.5750745347433863
1108	9	0.3490392217568328
1108	10	0.6210889008041249
1108	11	0.39667787862452997
1108	12	0.49052892705644524
1108	13	0.46878199174425816
1108	14	-0.8993456360218843
1108	15	0.13728306817231584
1108	16	0.43775899414869457
1108	17	0.585496223851365
1108	18	0.7566593737859932
1108	19	0.7955401790732992
1108	20	0.8196599470920702
1108	21	0.8063953697303887
1108	22	0.8860344442279171
1108	23	1.1879651523442263
1108	24	0.991741940082008
1108	25	0.9951597776448864
1109	1	0.3005939379278928
1109	2	0.21604001443779453
1109	3	0.3456335981444596
1109	4	0.32768231486065247
1109	5	0.43659235237944927
1109	6	0.13547735663124882
1109	7	0.12227818347856356
1109	8	0.2711146627445089
1109	9	0.3297934841681922
1109	10	0.3672231955610741
1109	11	0.19081231150403966
1109	12	0.15021734746077442
1109	13	0.18459136449233426
1109	14	0.6044976243046136
1109	15	0.3068941229588589
1109	16	0.3883703280107369
1109	17	0.2522273665834831
1109	18	0.32076494244621045
1109	19	0.3064478990490471
1109	20	0.4971806131145371
1109	21	0.438231941301936
1109	22	0.6454613374835176
1109	23	0.9822675966041245
1109	24	0.7141671005787273
1109	25	0.5466950042762733
1110	1	0.28462556637039427
1110	2	0.11084830803762813
1110	3	0.2729937514864851
1110	4	0.3785824322260254
1110	5	0.3669027231732045
1110	6	0.1119401968403082
1110	7	0.11012101120054751
1110	8	0.15399251450453733
1110	9	0.3015513997327588
1110	10	0.3368834628044226
1110	11	0.09603593666724208
1110	12	0.0810526870574435
1110	13	0.07774210930470815
1110	14	0.3977654870588836
1110	15	0.27555360914099936
1110	16	0.23552959933919482
1110	17	0.2607052209827856
1110	18	0.36245480588834056
1110	19	0.26428321307771085
1110	20	0.367395541033396
1110	21	0.444808856035948
1110	22	0.474963017136254
1110	23	0.7750801839786448
1110	24	0.5822099030626191
1110	25	0.6032749294362603
1111	1	0.19026510903865557
1111	2	0.08176924574823374
1111	3	0.22928204976098598
1111	4	0.4495658757427634
1111	5	0.2728630072178788
1111	6	0.14814970389852533
1111	7	0.1259536254608875
1111	8	0.25650027582828017
1111	9	0.19296424250128297
1111	10	0.26777269691842776
1111	11	0.1351335877655373
1111	12	0.22366794207442808
1111	13	0.10711855048926822
1111	14	-1.6273192216452728
1111	15	-0.5916170984596789
1111	16	-0.11216007069926093
1111	17	0.01790321065310252
1111	18	0.1399597585898249
1111	19	0.25095847839889984
1111	20	0.2934254053273406
1111	21	0.4092244070390194
1111	22	0.21005077032123154
1111	23	-1.7305128176836604
1111	24	-0.3489942383773652
1111	25	-0.01720984073566001
1112	1	-0.22942863379402922
1112	2	-0.3634097839331112
1112	3	-0.04101538928276938
1112	4	0.08602693833253028
1112	5	-0.6668123235077488
1112	6	-0.9339392167039691
1112	7	-0.9378012340775399
1112	8	-0.7966858942178376
1112	9	-0.6983359909160214
1112	10	-0.9449984074813997
1112	11	-0.7862646702908975
1112	12	-0.8628171348627163
1112	13	-0.8782365053550123
1112	14	-0.6263938630004375
1112	15	-0.9272913685367286
1112	16	-0.9146375274446896
1112	17	-0.8862322403860219
1112	18	-1.0220493803042814
1112	19	-0.814709376778211
1112	20	-0.9832543761663786
1112	21	-1.9825055648751544
1112	22	-1.7612851659421267
1112	23	-1.5324987175792302
1112	24	-1.6742073558076527
1112	25	-1.979284345063121
1113	1	-0.003843659764029894
1113	2	-0.12835443667172441
1113	3	-0.11798059147023565
1113	4	0.19160284436538433
1113	5	0.08747858537902431
1113	6	-0.05269489422649798
1113	7	-0.009470890329680515
1113	8	-0.020487492134058982
1113	9	0.2118136500831156
1113	10	-0.11502420606360175
1113	11	-0.08761729147937558
1113	12	-0.06577075976600355
1113	13	-0.06947491148059015
1113	14	-0.023814024829000857
1113	15	-0.17971113841449693
1113	16	-0.05822774860317281
1113	17	-0.030268142677797982
1113	18	-0.04343470682936612
1113	19	-0.11001041804411216
1113	20	-0.6492410767045413
1113	21	-0.5483118978777526
1113	22	-0.0894135397513125
1113	23	-0.2245506471499823
1113	24	0.026271064744925354
1113	25	-1.124625964764192
1114	1	-0.8018821274151371
1114	2	-0.7302521026395432
1114	3	-0.4776353493534522
1114	4	-1.4848591900786303
1114	5	-0.7559529765058599
1114	6	-0.5325736424572227
1114	7	-0.48701602901005003
1114	8	-0.3512303776619774
1114	9	-0.3448881083653461
1114	10	-0.47006577769159463
1114	11	-0.5530039342061457
1114	12	-0.4787356646097152
1114	13	-0.5322346585980284
1114	14	-0.4868725620333265
1114	15	-1.2785059577875302
1114	16	-0.8672647628960561
1114	17	-0.7477365119630832
1114	18	-0.7105820831722909
1114	19	-0.7092153120684728
1114	20	-1.7671686342616866
1114	21	-6.681020546976022
1114	22	-7.5957277409197985
1114	23	-7.700899863764636
1114	24	-5.766357459466949
1114	25	-7.094200263587894
1115	1	-1.021921954137376
1115	2	-1.0491343512179334
1115	3	-0.8053284520902011
1115	4	-0.614145412174139
1115	5	-0.7093118336036355
1115	6	-0.9437100949709105
1115	7	-1.0190881786000148
1115	8	-0.8233233462819953
1115	9	-2.862773909139328
1115	10	-1.7131605440985282
1115	11	-1.336406658838854
1115	12	-1.1195181918459625
1115	13	-1.3392518996427611
1115	14	-1.1634338826717538
1115	15	-2.0414583042839
1115	16	-1.4574394989619464
1115	17	-3.3034513649866923
1115	18	-2.9250009837487383
1115	19	-2.177372923294854
1115	20	-1.8017764423896807
1115	21	-1.3618125452534073
1115	22	-0.856992663922675
1115	23	-0.3774413500261602
1115	24	-1.3134068347257184
1115	25	-1.0147475349264852
1116	1	-1.5005130027720526
1116	2	-0.8108082888492163
1116	3	-0.5506831135864054
1116	4	-0.29260719921566647
1116	5	-0.30633881696120824
1116	6	-0.3786753559177569
1116	7	-0.7124150488536927
1116	8	-1.6646547406914323
1116	9	-0.8328821657235892
1116	10	-2.3470270815036045
1116	11	-1.0917790938041136
1116	12	-0.6662947308668095
1116	13	-0.8071788668426336
1116	14	-1.0876542736880737
1116	15	-1.106896985815652
1116	16	-0.7549027401963926
1116	17	-0.5955825655037401
1116	18	-1.2017823700793286
1116	19	-0.8324060465781002
1116	20	-1.0543212800047996
1116	21	-0.5274289614757421
1116	22	-0.14896228744828477
1116	23	2.5019693126182574E-6
1116	24	-0.1250080627108474
1116	25	-0.10199911030215247
1201	1	0.3507708636462219
1201	2	0.4546564426704478
1201	3	0.29781864589084606
1201	4	0.40494388130730385
1201	5	0.429379290401279
1201	6	0.34315401330808726
1201	7	0.3346524787686107
1201	8	0.40979863093326685
1201	9	0.7202264970534031
1201	10	0.6682621082191105
1201	11	0.47058402131909816
1201	12	0.5229395468580051
1201	13	0.5208924042299898
1201	14	1.185557946615404
1201	15	0.955518326353122
1201	16	0.8918473340840194
1201	17	0.7468517617442956
1201	18	0.689605152700075
1201	19	0.7420055409365816
1201	20	0.8180801961964761
1201	21	0.9284418559988268
1201	22	0.9443177598935151
1201	23	0.8092729090506765
1201	24	0.8366524792965677
1201	25	0.7671976526162183
1202	1	-0.20624047544655966
1202	2	0.035287212371478915
1202	3	-0.0038671241427294945
1202	4	-0.05851122892124749
1202	5	0.010920078692102209
1202	6	-0.043541731219050916
1202	7	-0.0653678266682931
1202	8	0.09167416216626378
1202	9	0.2045482959555187
1202	10	0.359105665247597
1202	11	0.11111718181550856
1202	12	0.050292582796160445
1202	13	0.10599168901236311
1202	14	0.28545304618277356
1202	15	0.31498800162737695
1202	16	0.26243741806009524
1202	17	0.21503980618134477
1202	18	0.15162625327387502
1202	19	0.24580687502159293
1202	20	0.3909315566722924
1202	21	0.2847296646943036
1202	22	0.294079306203642
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2211	3	0.3809940086146497
2211	4	0.4492220464702896
2211	5	0.3538440249400878
2211	6	0.22419235385990532
2211	7	0.2731735986717396
2211	8	0.3151530672348599
2211	9	0.49777653607416994
2211	10	0.35864571131085654
2211	11	0.25673159532802003
2211	12	0.28943411288072696
2211	13	0.11446286811849404
2211	14	0.5374698055422726
2211	15	0.3232152506979915
2211	16	0.2947201059134201
2211	17	0.3586750195339832
2211	18	0.3932788381658554
2211	19	0.369407999597847
2211	20	0.40989763615176855
2211	21	0.4616092452200604
2211	22	0.5159505956238348
2211	23	0.585061280296955
2211	24	0.6219259669065877
2211	25	0.6703298278865049
2212	1	-0.14513494164041418
2212	2	-0.17855738112504582
2212	3	-0.09875630726235585
2212	4	-0.3736731608558088
2212	5	-0.10867193150230037
2212	6	-0.5000394494042197
2212	7	-0.45420637815721676
2212	8	-0.19142307829866212
2212	9	-0.18928763326909603
2212	10	-0.48369891913883833
2212	11	-0.3558920418706961
2212	12	-0.45485251640548086
2212	13	-0.5353398685403263
2212	14	-0.29987621422802135
2212	15	-0.4792933588286914
2212	16	-0.2942347897204556
2212	17	-0.23219366802788954
2212	18	-0.2533898816452904
2212	19	-0.31454118737487846
2212	20	-0.3425137433594827
2212	21	-0.2925923282668137
2212	22	-0.42143609457269804
2212	23	0.05793614119669144
2212	24	-0.1373114171877461
2212	25	-0.6892013375236061
2213	1	-0.07880708718455409
2213	2	-1.2721763398035755
2213	3	-0.162392323034112
2213	4	0.1391302459253012
2213	5	0.1265062589761783
2213	6	0.09800732100715237
2213	7	0.17268828214034926
2213	8	0.2633958458269632
2213	9	0.25219731632410713
2213	10	-0.1656012762748631
2213	11	0.045038997711699835
2213	12	0.12345111901372974
2213	13	-0.2528116292452651
2213	14	0.0508535361958522
2213	15	-0.010775472014046272
2213	16	0.08731641566034654
2213	17	0.1940171354800242
2213	18	0.08169161731706254
2213	19	0.10485518845683828
2213	20	0.2002949503203837
2213	21	-0.339305995153218
2213	22	-0.7159337463851543
2213	23	0.09330591847907499
2213	24	0.19703289333245522
2213	25	-0.48684867573908264
2214	1	-0.13425071561796287
2214	2	-0.3763781182529975
2214	3	-0.37546591460103684
2214	4	-0.4167984948197514
2214	5	-0.18794271763523795
2214	6	-0.19055816942302073
2214	7	-0.3615866672535972
2214	8	-0.06904527958874951
2214	9	0.01462487058642381
2214	10	-1.7946498765635255
2214	11	-0.7717792153698895
2214	12	-0.4320850765699831
2214	13	-0.23395890816344433
2214	14	-0.735798485460478
2214	15	-0.5208970732726854
2214	16	-0.6869418199766173
2214	17	-0.5370736355555934
2214	18	-0.5194653404496563
2214	19	-1.3494096199839944
2214	20	-2.433448642182931
2214	21	-1.8363363395815
2214	22	-1.9722536723002335
2214	23	-2.413962723236189
2214	24	-1.5475799690558745
2214	25	-1.8148012448967137
2215	1	-0.19567987275857135
2215	2	-0.34204469191419307
2215	3	-0.744884554627113
2215	4	-0.2972666789172038
2215	5	-0.14634581948710235
2215	6	-0.18235088568606983
2215	7	-0.20747305345233968
2215	8	-0.18548332497130104
2215	9	-0.18289240235810666
2215	10	-0.3141113823080701
2215	11	-0.5226324643540963
2215	12	-0.4056072348965998
2215	13	-0.3449972075522538
2215	14	-0.15762787421682845
2215	15	-0.5896340383995877
2215	16	-0.4389657516267462
2215	17	-0.2858284784920855
2215	18	-0.27112422769069866
2215	19	-2.155199371110754
2215	20	-2.4573527366560555
2215	21	-3.210810934877955
2215	22	-2.312014869841633
2215	23	-3.283035150607276
2215	24	-2.289524933378104
2215	25	-1.6523255908701415
2216	1	-1.5115105455535023
2216	2	-1.150475963384416
2216	3	-0.7538547204974257
2216	4	-0.9060423402974465
2216	5	-1.8553259488390808
2216	6	-1.4776312277367225
2216	7	-0.9754248648566275
2216	8	-5.045244721244959
2216	9	-4.044297773107921
2216	10	-1.9589682763876013
2216	11	-1.094295022889554
2216	12	-1.132734329935218
2216	13	-1.896868392730056
2216	14	-3.398953363835318
2216	15	-2.069190706784596
2216	16	-2.9575402806356337
2216	17	-2.61940324575815
2216	18	-2.6428279397374084
2216	19	-1.7167022107030263
2216	20	-0.922025696199988
2216	21	-0.7544830410280241
2216	22	-0.7150771242742948
2216	23	-1.1121843992307987
2216	24	-0.5305337575573077
2216	25	-0.35957034163148194
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	3.0
7	92.0
8	489.0
9	1224.0
10	2095.0
11	3816.0
12	6023.0
13	8857.0
14	12896.0
15	18597.0
16	23549.0
17	25606.0
18	26107.0
19	26763.0
20	27228.0
21	28486.0
22	29667.0
23	30031.0
24	31609.0
25	33451.0
26	34702.0
27	37728.0
28	41609.0
29	48437.0
30	58157.0
31	70346.0
32	94293.0
33	133791.0
34	196819.0
35	314808.0
36	559077.0
37	1864156.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.31127410166434	19.437665877693398	11.202795862996801	24.048264157645463
2	12.186558951257616	23.448602858004527	36.82474372603389	27.54009446470397
3	21.38706497382956	29.715217696473562	26.894177275044512	22.003540054652365
4	13.197091401522378	20.186826023707763	33.21730920601447	33.39877336875539
5	11.161278184676107	41.113483866218125	34.80755220420389	12.917685744901883
6	31.63022976080951	33.62349706173276	18.577347282792093	16.168925894665634
7	23.694606415147902	34.50786789803681	22.694280861508847	19.10324482530644
8	26.523399709744456	36.331567757204745	21.60910582455448	15.53592670849632
9	26.729314927548657	18.682961897915167	20.461076777976377	34.12664639655979
10	16.08439427684096	32.91647473917648	31.87122839397714	19.12790259000542
11	28.06557063926255	28.807746360154674	19.48604357710125	23.64063942348153
12	23.20888958303763	32.38585547647302	28.154259666622167	16.250995273867183
13	28.114134565638537	23.21753122293329	22.70085792988239	25.967476281545785
14	20.961400515131153	28.015315166293757	25.79309646164573	25.230187856929355
15	30.957319444349174	25.535307529103495	21.49165874761703	22.015714278930297
16	18.270536945065526	30.06668117691288	31.61294577784813	20.04983610017346
17	25.346596560284716	30.62167089222067	27.201399313534345	16.830333233960264
18	25.246832544327496	26.752099501269516	28.81025974930509	19.190808205097902
19	22.466402849228334	32.2819944598338	29.283128874818626	15.968473816119245
20	23.116093992094424	28.654112965372015	27.552065182091283	20.67772786044228
21	18.546757278702774	30.903006430469134	30.613775261520004	19.936461029308088
22	21.198821145037602	30.17151061782688	22.611345396477375	26.018322840658136
23	17.340813665688497	31.46832854811037	27.47361350430934	23.71724428189179
24	20.27874101167348	36.25601244383285	24.453735576994102	19.01151096749956
25	21.603509057697007	27.669950150141894	22.219307644385307	28.507233147775796
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	14139.0
1	14139.0
2	16691.5
3	19244.0
4	19244.0
5	19244.0
6	22867.5
7	26491.0
8	26491.0
9	26491.0
10	27226.0
11	27961.0
12	27961.0
13	27961.0
14	33057.5
15	38154.0
16	38154.0
17	38154.0
18	50815.5
19	63477.0
20	63477.0
21	63477.0
22	83328.0
23	103179.0
24	103179.0
25	103179.0
26	129273.0
27	155367.0
28	155367.0
29	155367.0
30	180676.0
31	205985.0
32	205985.0
33	205985.0
34	226877.5
35	247770.0
36	247770.0
37	247770.0
38	264266.0
39	280762.0
40	280762.0
41	280762.0
42	326629.0
43	372496.0
44	372496.0
45	372496.0
46	669003.0
47	965510.0
48	965510.0
49	965510.0
50	819446.0
51	673382.0
52	673382.0
53	673382.0
54	478819.5
55	284257.0
56	284257.0
57	284257.0
58	213424.0
59	142591.0
60	142591.0
61	142591.0
62	115085.5
63	87580.0
64	87580.0
65	87580.0
66	67211.0
67	46842.0
68	46842.0
69	46842.0
70	34308.5
71	21775.0
72	21775.0
73	21775.0
74	15293.5
75	8812.0
76	8812.0
77	8812.0
78	5987.0
79	3162.0
80	3162.0
81	3162.0
82	2125.5
83	1089.0
84	1089.0
85	1089.0
86	724.5
87	360.0
88	360.0
89	360.0
90	223.5
91	87.0
92	87.0
93	87.0
94	53.0
95	19.0
96	19.0
97	19.0
98	20.0
99	21.0
100	21.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03163161071644147
2	0.013850371664830503
3	0.019522428632332518
4	0.020656840025832922
5	0.019284993689506855
6	0.00931272609082889
7	0.0012135563744422916
8	0.001556517958523809
9	9.761214316166259E-4
10	4.7486988565133157E-4
11	9.233581109887003E-4
12	0.0025854027107683605
13	0.002875600974221952
14	5.540148665932202E-4
15	0.0019522428632332519
16	0.0022952044473147693
17	0.0021632961457449546
18	0.002321586107628732
19	3.1657992376755436E-4
20	6.067781872211458E-4
21	0.08521276281410005
22	0.08199420025579658
23	0.08331328327149472
24	0.0010552664125585145
25	0.2309978177090588
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3790512.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.05653316954372
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.17511887688843	49.286108378056106
2	6.667411066204452	7.208342549105276
3	1.0822277723636498	1.7550444442133113
4	0.34816565208697203	0.7528251248214088
5	0.1588387696138015	0.42931366091189865
6	0.10566366141936953	0.34270867309989517
7	0.06795629562485549	0.2571437223967023
8	0.04933498724619431	0.2133502699594335
9	0.041578014655794554	0.202280699552826
>10	0.2465664225643225	2.4947183042024674
>50	0.026751316596364885	0.9969394719265242
>100	0.021826041434670488	2.405599226651337
>500	0.003315579544424058	1.2997828410394023
>1k	0.003266093282504415	4.012476630963505
>5k	4.453763567051475E-4	1.8576122177082572
>10k+	0.0015340741175399527	26.48575378539159
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	158225	4.174238203176774	No Hit
CCCATGTACTCTGCGTTGATACCAC	148424	3.9156715504396242	No Hit
GAGTACATGGGAAGCAGTGGTATCA	74410	1.9630593439619766	No Hit
CATGTACTCTGCGTTGATACCACTG	57744	1.5233825931694716	No Hit
GCGTTGATACCACTGCTTCCCATGT	49081	1.2948382698696113	No Hit
GCTTCCCATGTACTCTGCGTTGATA	43344	1.1434866846484064	No Hit
GTATCAACGCAGAGTACATGGGAAG	41601	1.0975034507211692	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	40339	1.0642097954049479	No Hit
ACTCTGCGTTGATACCACTGCTTCC	32149	0.8481439974335921	No Hit
TATCAACGCAGAGTACATGGGAAGC	31402	0.8284368971790619	No Hit
GGTATCAACGCAGAGTACATGGGAA	26816	0.7074506029792281	No Hit
CAGTGGTATCAACGCAGAGTACATG	21964	0.5794467871358803	No Hit
GTGGTATCAACGCAGAGTACATGGG	20709	0.5463378034418569	No Hit
GCAGTGGTATCAACGCAGAGTACAT	17919	0.4727329711659006	No Hit
ATACCACTGCTTCCCATGTACTCTG	16568	0.4370913480817367	No Hit
CATGGGAAGCAGTGGTATCAACGCA	16211	0.427673095349652	No Hit
GTACTCTGCGTTGATACCACTGCTT	15735	0.41511542504020565	No Hit
ACATGGGAAGCAGTGGTATCAACGC	15321	0.40419341767022504	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	14815	0.39084429755135985	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	14713	0.3881533681993356	No Hit
GTACATGGTAAGCAGTGGTATCAAC	14396	0.37979038187980935	No Hit
TACCACTGCTTCCCATGTACTCTGC	12782	0.3372103821330733	No Hit
GATACCACTGCTTCCCATGTACTCT	12575	0.331749378448083	No Hit
CTTCCCATGTACTCTGCGTTGATAC	12466	0.32887377747386104	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	12449	0.3284252892485237	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	12343	0.3256288332552436	No Hit
GTTGATACCACTGCTTCCCATGTAC	12081	0.31871683825298536	No Hit
CCACTGCTTCCCATGTACTCTGCGT	11528	0.30412778009936386	No Hit
CCATGTACTCTGCGTTGATACCACT	11133	0.29370702427534856	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	10621	0.28019961419459954	No Hit
ATGGGAAGCAGTGGTATCAACGCAG	10237	0.2700690566340378	No Hit
GTACATGGGTAAGCAGTGGTATCAA	9785	0.2581445461721266	No Hit
ACCATGTACTCTGCGTTGATACCAC	9637	0.2542400604456601	No Hit
TTCCCATGTACTCTGCGTTGATACC	9230	0.24350272469787723	No Hit
TGATACCACTGCTTCCCATGTACTC	8398	0.22155318331666013	No Hit
CCCCATGTACTCTGCGTTGATACCA	7593	0.20031594676392003	No Hit
GGTAAGCAGTGGTATCAACGCAGAG	7093	0.1871251166069386	No Hit
ATCAACGCAGAGTACATGGGAAGCA	6704	0.17686265074480703	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	5686	0.15000612054519286	No Hit
GCTTACCATGTACTCTGCGTTGATA	5316	0.14024490622902658	No Hit
AAAAAGTACTCTGCGTTGATACCAC	4919	0.12977138708438332	No Hit
GAGTACATGGTAAGCAGTGGTATCA	4740	0.12504906988818398	No Hit
GGAAGCAGTGGTATCAACGCAGAGT	4714	0.12436314672002094	No Hit
TCCATGTACTCTGCGTTGATACCAC	3916	0.10331058178947856	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	2.6381660313962864E-5	0.0	0.0	0.0
6	0.0	2.6381660313962864E-5	0.0	0.0	0.0
7	0.0	2.6381660313962864E-5	0.0	0.0	0.0
8	0.0	2.6381660313962864E-5	0.0	2.6381660313962864E-5	0.0
9	0.0	2.6381660313962864E-5	0.0	1.0552664125585146E-4	0.0
10	0.0	2.6381660313962864E-5	0.0	2.638166031396286E-4	0.0
11	0.0	2.6381660313962864E-5	0.0	2.638166031396286E-4	0.0
12	0.0	2.6381660313962864E-5	0.0	3.1657992376755436E-4	0.0
13	0.0	2.6381660313962864E-5	0.0	4.2210656502340583E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACAAA	920	0.0	12.598096	1
CGCAAAA	335	0.0	12.4757395	2
GTCTGCG	130	2.0008883E-10	12.417771	9
TCAGGCG	60	0.005885446	11.081525	3
CCGTACT	60	0.0058894935	11.080501	4
AAGCGGT	170	1.4006218E-10	10.613113	10
GAACAAA	1795	0.0	10.53226	1
CTAGTAC	160	6.199116E-9	10.092103	3
ACGCAAA	510	0.0	10.059041	1
GAAGCGG	185	6.548362E-10	9.75259	9
GTCTTAC	245	0.0	9.694087	1
GTCTTAG	300	0.0	9.500205	1
ATCGGTC	80	0.0045358986	9.495442	14
GTACAAG	515	0.0	9.407969	1
GTATTAG	425	0.0	9.388437	1
CCTACAC	235	7.2759576E-12	9.296355	3
CGGTGGT	225	2.7284841E-11	9.2845545	13
TAGGACC	185	6.7375368E-9	9.240881	4
GTCCTAG	280	0.0	9.160912	1
CGGCTTC	135	6.894197E-6	9.14388	13
>>END_MODULE