FastQCFastQC Report
Wed 25 May 2016
SRR1294697_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294697_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3790512
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC1655844.368380841427227No Hit
GTACATGGGAAGCAGTGGTATCAAC1530904.038768377464574No Hit
GAGTACATGGGAAGCAGTGGTATCA805382.1247261583659407No Hit
CATGTACTCTGCGTTGATACCACTG668491.7635876103281034No Hit
GCGTTGATACCACTGCTTCCCATGT536101.414320809431549No Hit
ACGCAGAGTACATGGGAAGCAGTGG493401.3016711198909277No Hit
GCTTCCCATGTACTCTGCGTTGATA489541.291487799009738No Hit
GTATCAACGCAGAGTACATGGGAAG431241.1376827193793344No Hit
ACTCTGCGTTGATACCACTGCTTCC383591.0119741079833016No Hit
TATCAACGCAGAGTACATGGGAAGC353010.931298990743203No Hit
GGTATCAACGCAGAGTACATGGGAA290820.767231445250668No Hit
CAGTGGTATCAACGCAGAGTACATG261730.69048719539735No Hit
GTGGTATCAACGCAGAGTACATGGG215320.5680499098802484No Hit
CATGGGAAGCAGTGGTATCAACGCA201110.5305615705741071No Hit
GCAGTGGTATCAACGCAGAGTACAT198140.5227262174608602No Hit
ATACCACTGCTTCCCATGTACTCTG187820.4955003440168505No Hit
ACATGGGAAGCAGTGGTATCAACGC186190.49120013338567453No Hit
GGGAAGCAGTGGTATCAACGCAGAG171820.45328968751450993No Hit
CTGCTTCCCATGTACTCTGCGTTGA168510.4445573579505882No Hit
GTACTCTGCGTTGATACCACTGCTT158690.4186505675222767No Hit
CTTCCCATGTACTCTGCGTTGATAC152670.40276880801327103No Hit
GATACCACTGCTTCCCATGTACTCT147930.39026390102445263No Hit
AAGCAGTGGTATCAACGCAGAGTAC146650.3868870485042654No Hit
TACCACTGCTTCCCATGTACTCTGC144930.38234940293026376No Hit
CCACTGCTTCCCATGTACTCTGCGT136270.3595028850983719No Hit
GCAGAGTACATGGGAAGCAGTGGTA135270.35686471906697564No Hit
GTACATGGTAAGCAGTGGTATCAAC133830.353065759981765No Hit
CCATGTACTCTGCGTTGATACCACT133660.35261727175642765No Hit
GTTGATACCACTGCTTCCCATGTAC126130.3327518815400136No Hit
ATGGGAAGCAGTGGTATCAACGCAG122030.3219354008112888No Hit
ACCATGTACTCTGCGTTGATACCAC109800.2896706302473122No Hit
GTACATGGGTAAGCAGTGGTATCAA108840.28713799085717184No Hit
GTATCAACGCAGAGTACTTTTTTTT105390.2780363180488546No Hit
TTCCCATGTACTCTGCGTTGATACC94490.2492803083066351No Hit
CCCCATGTACTCTGCGTTGATACCA90490.23872764418104994No Hit
TGATACCACTGCTTCCCATGTACTC88810.23429552524830416No Hit
GGTAAGCAGTGGTATCAACGCAGAG80450.21224045722583124No Hit
TATCAACGCAGAGTACTTTTTTTTT79630.2100771610800863No Hit
GGTATCAACGCAGAGTACTTTTTTT78640.20746537670900395No Hit
ATCAACGCAGAGTACATGGGAAGCA78250.2064364919567594No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA76980.20308602109688612No Hit
GCTTACCATGTACTCTGCGTTGATA60390.15931884663602172No Hit
GTACTTTTTTTTTTTTTTTTTTTTT60250.15894950339162625No Hit
ACGCAGAGTACTTTTTTTTTTTTTT58420.15412165955417106No Hit
GGAAGCAGTGGTATCAACGCAGAGT55520.14647097806312182No Hit
AAAAAGTACTCTGCGTTGATACCAC55500.1464182147424939No Hit
GAGTACATGGTAAGCAGTGGTATCA53580.14135293596221302No Hit
CTGCGTTGATACCACTGCTTCCCAT46040.12146116408548503No Hit
GAGTACTTTTTTTTTTTTTTTTTTT44290.11684437353054151No Hit
TGGGAAGCAGTGGTATCAACGCAGA43330.11431173414040109No Hit
CTCTGCGTTGATACCACTGCTTCCC42390.11183185807088858No Hit
TCCATGTACTCTGCGTTGATACCAC41930.11061830169644628No Hit
ACTCTGCGTTGATACCACTGCTTAC40880.10784822736348018No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGT400.005231601714.2701834
ACCGGGC500.001506795413.2923098
ACGCTGA853.862666E-612.3180431
AAAAAGT11250.010.6607061
AAAAGTA11600.010.4155932
ATTAACG1105.9501344E-610.3783143
TTAACGC1105.9501344E-610.3783144
GTATTAG3050.010.2986921
TACACCG750.002618153310.1476855
GTATTAA4800.010.1133931
TAGACAG3650.09.9044195
TTAGACA2550.09.6999934
CAGGACG800.0044655369.5134554
TTAGGCT2401.8189894E-129.5134544
AGTACTC14850.09.3532955
CTAGTAC1752.6126145E-89.2416433
CGCAAAA1752.6126145E-89.2416432
TCAGGAC3300.09.2251683
AAGTACT16000.09.216164
CGAACTC1554.3011096E-79.18823115
Produced by FastQC (version 0.11.5)