FastQCFastQC Report
Wed 25 May 2016
SRR1294696_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294696_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4745651
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC307410.6477720338052672No Hit
CCCATGTACTCTGCGTTGATACCAC299500.6311041414549869No Hit
GAGTACATGGGAAGCAGTGGTATCA139410.293763700701969No Hit
CATGTACTCTGCGTTGATACCACTG113300.23874490559883144No Hit
GCGTTGATACCACTGCTTCCCATGT101340.21354288378981093No Hit
GTATCAACGCAGAGTACATGGGAAG79920.16840682131914042No Hit
ACGCAGAGTACATGGGAAGCAGTGG76830.16189559662099046No Hit
ACTCTGCGTTGATACCACTGCTTCC67780.14282550486750922No Hit
TATCAACGCAGAGTACATGGGAAGC65430.13787360258898093No Hit
GCTTCCCATGTACTCTGCGTTGATA59530.12544116708118655No Hit
GGTATCAACGCAGAGTACATGGGAA51760.10906828167515902No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGAC1157.0960596E-811.5640363
GACCCGT600.005900239611.0778826
GTCCTAA3400.010.6202581
GTCGTAC1355.676702E-810.558151
GTCCTAG4550.010.4421271
TTAGACG750.00265384810.1312314
GTCTTAG4800.09.8982661
GTGCTAG4050.09.6196491
GTTCTAG4500.09.5023361
GCGGGAC1401.0614385E-69.499033
CGAATTA1252.7405751E-59.12455115
ACCCGTG1252.774842E-59.1149377
TCGTACC1151.11178255E-49.0867952
CCGGACT1804.2398824E-88.9703614
CGCAATC850.00739497648.94771517
ACTATCG2553.6379788E-128.93611911
TAGACTG4300.08.8350045
CCCCGCG1401.0938858E-58.81653212
CTTCTAG6600.08.7824621
TCTATAC5200.08.7683353
Produced by FastQC (version 0.11.5)