FastQCFastQC Report
Wed 25 May 2016
SRR1294696_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294696_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4745651
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC328420.692044147367769No Hit
GTACATGGGAAGCAGTGGTATCAAC304160.6409236583136855No Hit
GAGTACATGGGAAGCAGTGGTATCA153540.32353833014690714No Hit
CATGTACTCTGCGTTGATACCACTG127620.26891990161096974No Hit
GTATCAACGCAGAGTACTTTTTTTT127350.2683509596470537No Hit
GCGTTGATACCACTGCTTCCCATGT107390.22629139816644755No Hit
ACGCAGAGTACATGGGAAGCAGTGG95670.20159510254757462No Hit
GGTATCAACGCAGAGTACTTTTTTT91270.19232345572820253No Hit
TATCAACGCAGAGTACTTTTTTTTT86270.1817874934334615No Hit
GTATCAACGCAGAGTACATGGGAAG84710.1785002731975023No Hit
ACTCTGCGTTGATACCACTGCTTCC78900.16625748501101326No Hit
TATCAACGCAGAGTACATGGGAAGC75110.15827122559159953No Hit
GCTTCCCATGTACTCTGCGTTGATA64840.1366303590382015No Hit
GGTATCAACGCAGAGTACATGGGAA56990.12008889823545811No Hit
ACGCAGAGTACTTTTTTTTTTTTTT51530.10858362740960091No Hit
GTGGTATCAACGCAGAGTACATGGG50270.10592856491132617No Hit
CAGTGGTATCAACGCAGAGTACATG49670.10466424943595726No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTTAA600.00577902511.1088181
TCGACCG1158.9090645E-710.7316159
CTAGACA4200.010.4235094
TAACCCG856.4899604E-410.0769465
TACGACC2002.3646862E-119.9929734
TAGACAG6250.09.7455285
TAGAACC3150.09.6681824
CTAGGAC4150.09.6317813
ACGGGTC1502.5836925E-79.5171175
TAGGACA3300.09.5171174
GCGCGTC1201.7163366E-59.4933529
TGGACCG2151.03682396E-109.2957895
TACATCG2151.03682396E-109.2957895
CTAGCAC3900.09.2730883
TAGGACC3400.09.2372014
CGATACT1054.4155982E-49.0639214
GAACCGA950.00183479648.9946486
GATCGGT950.00183690838.99351111
CTATACT6050.08.9665394
TAAACGG850.0073304098.9572865
Produced by FastQC (version 0.11.5)