FastQCFastQC Report
Wed 25 May 2016
SRR1294695_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294695_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4472848
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC72810.1627821915701137No Hit
CCCATGTACTCTGCGTTGATACCAC70410.15741648274209183No Hit
GTATCAACGCAGAGTACTTTTTTTT55150.1232995174439194No Hit
ATTCCATTCCATTCCATTCCATTCC45580.10190375349218217No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTCGC1051.382432E-913.56545510
TCGCGCG853.967467E-612.2887079
ACCGTCT1459.822543E-1111.7877818
CGTCCGC1354.771209E-911.25430310
CGTACGG600.005881711.0825443
AAGCTCG600.005888557511.080815
ATCGTCG1201.2851342E-711.0784559
TATCGCG951.3684961E-510.9950367
TCGTCCG1303.870191E-710.2262679
CGCCCAT1701.5734258E-910.05439710
GTACCGC1803.9835868E-1010.0235896
GAACCGC1752.5975169E-99.7673466
CCGTATA1951.6370905E-109.7432232
TAGTCGC900.00111669799.4978375
CGCGCCT1502.668985E-79.49581912
ACCGCCC2106.730261E-119.4957128
CCGTACC1451.7115271E-69.1711454
TACGGAG1356.8743593E-69.1460645
GTATAGG5350.09.057761
CTGCGTA1054.51547E-49.0436379
Produced by FastQC (version 0.11.5)