##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294695_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4472848
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.17919298844942	33.0	33.0	33.0	27.0	33.0
2	31.211075583163122	33.0	33.0	33.0	27.0	33.0
3	31.180032721881002	33.0	33.0	33.0	27.0	33.0
4	34.74544876105783	37.0	37.0	37.0	27.0	37.0
5	34.8799793777924	37.0	37.0	37.0	33.0	37.0
6	35.05738916234131	37.0	37.0	37.0	33.0	37.0
7	35.05036925019585	37.0	37.0	37.0	33.0	37.0
8	34.93043783289752	37.0	37.0	37.0	33.0	37.0
9	34.69480854256617	37.0	37.0	37.0	27.0	37.0
10	34.77593604790505	37.0	37.0	37.0	27.0	37.0
11	34.979534739387525	37.0	37.0	37.0	33.0	37.0
12	34.921169912324316	37.0	37.0	37.0	33.0	37.0
13	34.85068573758822	37.0	37.0	37.0	33.0	37.0
14	36.02718223378036	40.0	37.0	40.0	27.0	40.0
15	36.32942277492998	40.0	37.0	40.0	27.0	40.0
16	36.35328229351858	40.0	37.0	40.0	27.0	40.0
17	36.29875573683702	40.0	37.0	40.0	27.0	40.0
18	36.297871065594	40.0	37.0	40.0	27.0	40.0
19	36.38804113173531	40.0	37.0	40.0	27.0	40.0
20	36.27115363634087	40.0	37.0	40.0	27.0	40.0
21	36.222672892081285	40.0	37.0	40.0	27.0	40.0
22	36.08375066624218	40.0	37.0	40.0	27.0	40.0
23	35.69665859425583	37.0	37.0	40.0	27.0	40.0
24	36.004560628932616	37.0	37.0	40.0	27.0	40.0
25	34.85481666267219	37.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.541182760090166
1101	2	0.6248342945265826
1101	3	0.4901493601163267
1101	4	0.6565340066660568
1101	5	0.6630545648619517
1101	6	0.6303160627849778
1101	7	0.6156877908393383
1101	8	0.6475958649274389
1101	9	0.849724540261036
1101	10	0.83369767532254
1101	11	0.7009375578103487
1101	12	0.6369361841117822
1101	13	0.6787670546795681
1101	14	1.4086160293463905
1101	15	1.152820563967559
1101	16	1.0705640270889845
1101	17	1.1411017886805723
1101	18	1.0816230750903273
1101	19	1.0229784821880443
1101	20	1.1456006124104476
1101	21	1.1429634707896241
1101	22	1.224460097607306
1101	23	1.5564775685776269
1101	24	1.2952098695773415
1101	25	1.238406805631783
1102	1	0.395260013309052
1102	2	0.5842150425339412
1102	3	0.451829311066664
1102	4	0.4836339453539722
1102	5	0.40017902838126673
1102	6	0.29325291134414755
1102	7	0.43328435736294324
1102	8	0.48312621440628334
1102	9	0.7356840742892388
1102	10	0.6745928316683347
1102	11	0.4858854425436405
1102	12	0.5391434189370443
1102	13	0.5290123029934861
1102	14	1.1417552077645396
1102	15	0.9471185406689742
1102	16	0.7175597352434906
1102	17	0.970654210507675
1102	18	0.8664176796274177
1102	19	0.7924450671052767
1102	20	0.9179795210554502
1102	21	1.0719947399496519
1102	22	1.091259607110679
1102	23	1.368096207449483
1102	24	1.1554183306441743
1102	25	1.0800683629585777
1103	1	0.354960229514802
1103	2	0.567910870085651
1103	3	0.42696500437719465
1103	4	0.20391469013438268
1103	5	0.37560030689945023
1103	6	0.3274014801911562
1103	7	0.3297043773700423
1103	8	0.4423736893167387
1103	9	0.6481139687679232
1103	10	0.7133220581680604
1103	11	0.46546335728758237
1103	12	0.49910630899481134
1103	13	0.47450618792039734
1103	14	1.175532354494024
1103	15	0.8446989724350829
1103	16	0.6215998785201364
1103	17	0.7025326189751908
1103	18	0.6864777009253018
1103	19	0.731271102149968
1103	20	0.8863586233175411
1103	21	0.9515942752686328
1103	22	0.8179626307642351
1103	23	1.3085279735600395
1103	24	0.9494852576182637
1103	25	0.9080860466527909
1104	1	0.3958240142924474
1104	2	0.5730861169235908
1104	3	0.3648225437835748
1104	4	0.45330455165491657
1104	5	0.3782621464634488
1104	6	0.3543033135890923
1104	7	0.38853874601338845
1104	8	0.5411615444098246
1104	9	0.7288198040737655
1104	10	0.688363181999776
1104	11	0.493583747402667
1104	12	0.43852500132062744
1104	13	0.49678709446546776
1104	14	1.0167601379624998
1104	15	0.7907941464866894
1104	16	0.7599641743436365
1104	17	0.7579900189749722
1104	18	0.6559329316331812
1104	19	0.7290013213244109
1104	20	0.8855770303513211
1104	21	0.8651771255752081
1104	22	1.0041915368580234
1104	23	1.369592892580215
1104	24	1.1233549191439351
1104	25	0.8765038822088442
1105	1	0.35666837688858166
1105	2	0.42709537323300495
1105	3	0.45936226312120354
1105	4	0.4232664116842457
1105	5	0.46110895373936955
1105	6	0.34042432250192434
1105	7	0.3615848360926037
1105	8	0.47231208253686674
1105	9	-1.4576698311954388
1105	10	0.021029026122711514
1105	11	0.24098330689421488
1105	12	0.41648201206710667
1105	13	0.4050805739746579
1105	14	-1.4226463364400814
1105	15	-0.08682824132123557
1105	16	0.1243214775265642
1105	17	0.4405377029864681
1105	18	0.3182952572939399
1105	19	0.494873378786302
1105	20	0.6613940282291111
1105	21	0.7143851204743399
1105	22	0.7973985199524449
1105	23	1.0842248360370874
1105	24	0.8810539744191743
1105	25	0.5271607387305792
1106	1	0.06865130301750355
1106	2	-0.00915708128203363
1106	3	0.08950392185554534
1106	4	-1.0661109895881182
1106	5	-0.27310093125824864
1106	6	-0.1250037334133367
1106	7	0.03765457112496051
1106	8	0.11068870444802315
1106	9	0.29710920870683566
1106	10	0.2731924291864374
1106	11	0.2280728008785431
1106	12	0.04226892202791532
1106	13	0.08465388865398893
1106	14	0.5656127122718217
1106	15	0.3253646359934663
1106	16	0.16054433469133755
1106	17	0.018345729291894486
1106	18	0.12498827702024329
1106	19	0.13921775153879423
1106	20	0.28521641183628077
1106	21	0.45866526305185573
1106	22	0.47786535258906326
1106	23	0.6900917614263449
1106	24	0.33231711582556045
1106	25	0.3041368101024773
1107	1	0.34698058617434313
1107	2	0.32594302482970505
1107	3	0.3162895666515375
1107	4	0.4848089515000922
1107	5	0.4966448919548583
1107	6	0.27899646835649605
1107	7	0.24527581116648633
1107	8	0.33602132473607327
1107	9	0.46839920235223786
1107	10	0.4863477077680898
1107	11	0.31073304645142485
1107	12	0.3394229449209689
1107	13	0.270603728505705
1107	14	0.7914780038850537
1107	15	0.5694715013947302
1107	16	0.5448788891112386
1107	17	0.5074282492949109
1107	18	0.5729591756336134
1107	19	0.4989068872346394
1107	20	0.6100334288163509
1107	21	0.6837115037115282
1107	22	0.6609540565141572
1107	23	0.8147479885035835
1107	24	0.6773352683295428
1107	25	0.5383507981771913
1108	1	0.36356959461319605
1108	2	0.3342993640997314
1108	3	0.41797280577133833
1108	4	0.58890959482207
1108	5	0.5023138304577728
1108	6	0.36697745600768883
1108	7	0.2849025807372314
1108	8	0.3550978830584981
1108	9	0.16000861141098
1108	10	0.47102171733877185
1108	11	0.28055605771746883
1108	12	0.3459470869076995
1108	13	0.3131437699163868
1108	14	-1.211275881928465
1108	15	-0.15326864021842113
1108	16	0.3052426414639413
1108	17	0.42241564088075734
1108	18	0.5535398037906631
1108	19	0.5234563916835953
1108	20	0.5389666525021184
1108	21	0.6283304494169855
1108	22	0.637980999579213
1108	23	0.9664546952617883
1108	24	0.7925180388880619
1108	25	0.5825166562062876
1109	1	0.25140672316094737
1109	2	0.1792622479628072
1109	3	0.31813139936580015
1109	4	0.36055307952442917
1109	5	0.35594829662404237
1109	6	0.2709636916221143
1109	7	0.14352885058485754
1109	8	0.25870112823184854
1109	9	0.4806519254644357
1109	10	0.4090709935029935
1109	11	0.25259985631039683
1109	12	0.12769796406892908
1109	13	0.22681691650121394
1109	14	0.5446048797344858
1109	15	0.3728328855379317
1109	16	0.29281274325860096
1109	17	0.3432326095873677
1109	18	0.32000031278980856
1109	19	0.2869850038167314
1109	20	0.4201413633685789
1109	21	0.3610945867738451
1109	22	0.5346403210171076
1109	23	0.9137204667111902
1109	24	0.5639228413619577
1109	25	0.4941770829836827
1110	1	0.29342686623392567
1110	2	0.17168278763663025
1110	3	0.31971529342007443
1110	4	0.43117338535431315
1110	5	0.3266989644149305
1110	6	0.15348043860580418
1110	7	0.18709084058979641
1110	8	0.18944135848967392
1110	9	0.25792831855031295
1110	10	0.327189046893551
1110	11	0.16917282684786983
1110	12	0.11410133819366308
1110	13	0.11785731794918775
1110	14	0.3907486027542433
1110	15	0.2711315573253188
1110	16	0.28479974891109805
1110	17	0.31150613012820116
1110	18	0.46629271348117385
1110	19	0.2511627018642386
1110	20	0.36057526187770605
1110	21	0.5314958902266937
1110	22	0.5079937811180812
1110	23	0.6441602962865431
1110	24	0.5788322784514435
1110	25	0.5422845694043303
1111	1	0.00423623176193999
1111	2	-0.010793956496932822
1111	3	0.14878274144111003
1111	4	0.3097472616375825
1111	5	0.20718709023496018
1111	6	0.11158874736653956
1111	7	-0.008800155260125564
1111	8	0.23654837333862133
1111	9	0.22462605129019408
1111	10	0.03091625043118995
1111	11	0.06585772617091123
1111	12	0.17181007005171267
1111	13	0.06452644120041384
1111	14	-1.8975124991620334
1111	15	-0.5829992640577188
1111	16	-0.1579693920508305
1111	17	0.04865138984778383
1111	18	0.1079761119653071
1111	19	0.08782559839886517
1111	20	0.1704426961961829
1111	21	0.24249721500009258
1111	22	0.023863652964067228
1111	23	-2.0663220132763627
1111	24	-0.46871690618662143
1111	25	-0.22177512405727384
1112	1	-0.07300757013107173
1112	2	-0.23130077500751156
1112	3	0.031379587512571305
1112	4	0.20529202258086343
1112	5	-0.4821651217557559
1112	6	-0.6925991328375432
1112	7	-0.7989781933045066
1112	8	-0.509877260111459
1112	9	-0.3889251434847836
1112	10	-0.698773856302175
1112	11	-0.6197294657810986
1112	12	-0.5579497228499903
1112	13	-0.6081578508965819
1112	14	-0.3966104566716808
1112	15	-0.7349102217693471
1112	16	-0.6804607152798994
1112	17	-0.7102647024980797
1112	18	-0.8541153430380533
1112	19	-0.6068821080258999
1112	20	-0.8533251764795438
1112	21	-1.6290145848351543
1112	22	-1.5889611197044786
1112	23	-1.248479015988842
1112	24	-1.5006068703780429
1112	25	-1.774618595098346
1113	1	-0.07433916003791907
1113	2	-0.1646778631582464
1113	3	-0.15063334835109998
1113	4	0.050100902553396054
1113	5	-0.12967304244902778
1113	6	-0.21599414580329324
1113	7	-0.1492768932338535
1113	8	-0.04981965711989034
1113	9	0.008810939410302865
1113	10	-0.22882563471559791
1113	11	-0.2608528481638075
1113	12	-0.12251350818299045
1113	13	-0.2688809105311236
1113	14	-0.11451350704779628
1113	15	-0.2241164270595064
1113	16	-0.16869016193369646
1113	17	-0.1549292930663242
1113	18	-0.07981188195305577
1113	19	-0.22750009971360896
1113	20	-0.857270158179773
1113	21	-0.7158404942277201
1113	22	-0.21643976526733866
1113	23	-0.3378456368144427
1113	24	-0.15282903433568862
1113	25	-1.1960055100397682
1114	1	-0.9465826222360079
1114	2	-0.6711611583511825
1114	3	-0.5483586585243714
1114	4	-1.4869401465850558
1114	5	-0.8086296722685802
1114	6	-0.6388495424834417
1114	7	-0.5662171807660954
1114	8	-0.43638365086864184
1114	9	-0.4146192814034251
1114	10	-0.5487957518721416
1114	11	-0.5214770802771653
1114	12	-0.5772755641821377
1114	13	-0.6291974443501829
1114	14	-0.596380194773225
1114	15	-1.353742559611831
1114	16	-0.9194249010539579
1114	17	-0.8332767638115186
1114	18	-0.7784827763954283
1114	19	-0.8122537837096928
1114	20	-1.9460302719657605
1114	21	-6.8425217761341806
1114	22	-7.973040113995886
1114	23	-8.043292703494618
1114	24	-5.955858863959989
1114	25	-7.4444976052780305
1115	1	-1.0732641776569736
1115	2	-0.9984569725979817
1115	3	-0.7931217509727198
1115	4	-0.6307180455509851
1115	5	-0.7094951239854552
1115	6	-0.9020837713993615
1115	7	-1.049277693190973
1115	8	-0.8244394974964493
1115	9	-2.7707350769232235
1115	10	-1.529725946458889
1115	11	-1.198382796558576
1115	12	-1.1441499084719382
1115	13	-1.4226945061224043
1115	14	-1.1843114858761297
1115	15	-1.9813970368188194
1115	16	-1.3853694679228923
1115	17	-3.167178196525093
1115	18	-2.838241790109983
1115	19	-2.24289315464587
1115	20	-1.7261919839699047
1115	21	-1.3098342961599414
1115	22	-0.8811698157366337
1115	23	-0.2972788511941573
1115	24	-1.3921804451000739
1115	25	-1.055817944093306
1116	1	-1.6056241399545996
1116	2	-0.8919227469404198
1116	3	-0.7098288543325495
1116	4	-0.3567071042315959
1116	5	-0.4336129811933489
1116	6	-0.3855114618340707
1116	7	-0.7995156742582807
1116	8	-1.8229810017587766
1116	9	-0.9267511550611331
1116	10	-2.5089223083704724
1116	11	-1.13447081096664
1116	12	-0.7677722066345964
1116	13	-0.8070410835566477
1116	14	-1.1383901579339692
1116	15	-1.2611715509498396
1116	16	-0.869579343898728
1116	17	-0.8236548887746906
1116	18	-1.4436793258244833
1116	19	-0.8478940521150022
1116	20	-1.1494757151523203
1116	21	-0.6091799240978517
1116	22	-0.22849722201613787
1116	23	-0.07419074227001232
1116	24	-0.13730961562180966
1116	25	-0.044416574217223115
1201	1	0.22103832067029217
1201	2	0.39929702778760046
1201	3	0.27076817746829107
1201	4	0.38858246817677156
1201	5	0.28275756656444884
1201	6	0.20977392714564758
1201	7	0.23149890894785585
1201	8	0.40948014323434023
1201	9	0.6124202094828348
1201	10	0.6007081705077368
1201	11	0.3871165853804399
1201	12	0.40681063768985126
1201	13	0.4218546495722606
1201	14	1.112766616619929
1201	15	0.7978145668509882
1201	16	0.766873696549375
1201	17	0.7012763107839035
1201	18	0.5751877112452206
1201	19	0.5287117626246811
1201	20	0.7135102126982318
1201	21	0.7965963732738288
1201	22	0.7999969019625937
1201	23	0.6698754599230057
1201	24	0.7613889988718512
1201	25	0.7275720920831077
1202	1	-0.07663515737331039
1202	2	0.16379436868943387
1202	3	0.0590221134419906
1202	4	0.188413867016763
1202	5	0.16203341061226695
1202	6	0.0761129643372982
1202	7	0.1000160660968703
1202	8	0.2594600127779856
1202	9	0.3454296691942105
1202	10	0.5353967255531984
1202	11	0.29686252024203696
1202	12	0.21943048719006697
1202	13	0.35579148436831787
1202	14	0.6701515627028272
1202	15	0.659958682597221
1202	16	0.553809007476751
1202	17	0.4259073783759746
1202	18	0.4522644575222259
1202	19	0.4239071562999186
1202	20	0.6004260573140101
1202	21	0.705612573367354
1202	22	0.5887550841113907
1202	23	0.929898801175689
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2211	5	0.16303798740866426
2211	6	0.16467532543292407
2211	7	0.16790186896797366
2211	8	0.2652842689090349
2211	9	0.345992081738153
2211	10	0.2703452634909098
2211	11	0.17721579378639518
2211	12	0.1793781393431857
2211	13	0.13088859305416634
2211	14	0.43037659081738866
2211	15	0.33040632819795945
2211	16	0.23573197220667197
2211	17	0.3096487577504732
2211	18	0.20468200230207856
2211	19	0.2898679672482487
2211	20	0.337844356830864
2211	21	0.3502075238936584
2211	22	0.3263188302667217
2211	23	0.558863415254315
2211	24	0.46444990685456844
2211	25	0.4725962432951718
2212	1	-0.4210129744153761
2212	2	-0.36061020214699724
2212	3	-0.3360884740545913
2212	4	-0.6492379740408225
2212	5	-0.3377629442357346
2212	6	-0.6982494756587911
2212	7	-0.5989578658803154
2212	8	-0.4483027208502577
2212	9	-0.38856801416723386
2212	10	-0.6804442386051264
2212	11	-0.5789075512598956
2212	12	-0.7795941942436997
2212	13	-0.7561408531303044
2212	14	-0.5558870986469628
2212	15	-0.8306418208559379
2212	16	-0.6181269410493115
2212	17	-0.7076395563861553
2212	18	-0.5815849445260071
2212	19	-0.7394782326590388
2212	20	-0.7205577385940032
2212	21	-0.6448238837685949
2212	22	-0.6703994237310695
2212	23	-0.11329986407379522
2212	24	-0.4146015112266497
2212	25	-1.137891174955172
2213	1	-0.1564717686045043
2213	2	-1.294761510388586
2213	3	-0.2940783432605798
2213	4	0.009201277030875588
2213	5	0.036726014650547256
2213	6	0.055010566499930746
2213	7	0.07087969244628312
2213	8	0.18065946735483607
2213	9	0.10505198152637263
2213	10	-0.36267285757917733
2213	11	-0.055374548287169034
2213	12	0.06313936460681191
2213	13	-0.33308807595869894
2213	14	-0.031589504206777974
2213	15	-0.016141244163648594
2213	16	0.008135773105564681
2213	17	0.10580323589906016
2213	18	0.15830906789598487
2213	19	0.12712193750544998
2213	20	0.21488602289757353
2213	21	-0.46837651816965575
2213	22	-0.87056891272087
2213	23	-0.11064918236634469
2213	24	0.08495306067120367
2213	25	-0.5366731486327225
2214	1	-0.28267276477083314
2214	2	-0.4928196445073354
2214	3	-0.5598903740582521
2214	4	-0.6102011035403052
2214	5	-0.3827303541285332
2214	6	-0.48454559515985096
2214	7	-0.6349580169421998
2214	8	-0.23491106173876375
2214	9	-0.22226501180843172
2214	10	-1.9876938046231416
2214	11	-1.06792298204752
2214	12	-0.6732503442453179
2214	13	-0.4243889880266565
2214	14	-0.9999123567919739
2214	15	-0.7141914823836188
2214	16	-0.8778241838307679
2214	17	-0.6828703123253845
2214	18	-0.6335154374347667
2214	19	-1.5061133527347508
2214	20	-2.5583735676761066
2214	21	-2.0739711748739538
2214	22	-2.132251670453755
2214	23	-2.669363702550548
2214	24	-1.727195629113666
2214	25	-2.1417934345432172
2215	1	-0.1490716230559741
2215	2	-0.261172725372667
2215	3	-0.5804120697232982
2215	4	-0.15085653029616708
2215	5	-0.12035942134807698
2215	6	-0.15993560279625285
2215	7	-0.14574271625827606
2215	8	-0.18483102542501229
2215	9	-0.18798958716701009
2215	10	-0.28837791493732823
2215	11	-0.4705910867540908
2215	12	-0.2694976269726368
2215	13	-0.24615892743408807
2215	14	-0.18084482484623976
2215	15	-0.5411921160949547
2215	16	-0.3425028354966315
2215	17	-0.18418939601562556
2215	18	-0.17952213536838713
2215	19	-2.043791515513945
2215	20	-2.2336778598031657
2215	21	-3.3089029241841175
2215	22	-2.460612890464965
2215	23	-3.19410465778504
2215	24	-2.077398125021851
2215	25	-1.3467946231398855
2216	1	-1.4731861209504018
2216	2	-1.1448481250048133
2216	3	-0.8500368753220542
2216	4	-0.902723137310943
2216	5	-1.8058497651986016
2216	6	-1.4837450505191825
2216	7	-1.1339613794878574
2216	8	-5.283324460166558
2216	9	-4.276767841923185
2216	10	-2.1510645933923414
2216	11	-1.1799618924806765
2216	12	-1.301054452497965
2216	13	-2.048021406751211
2216	14	-3.5116894671834515
2216	15	-2.175192494245316
2216	16	-2.9501696566190816
2216	17	-2.774208978168353
2216	18	-2.7394124096250465
2216	19	-1.7032701739855796
2216	20	-1.0037482792127577
2216	21	-0.8156879714440421
2216	22	-0.7760843445507675
2216	23	-1.1339856735714875
2216	24	-0.48182186292281415
2216	25	-0.3530220317624284
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	45.0
8	263.0
9	823.0
10	1800.0
11	3380.0
12	6403.0
13	9605.0
14	12818.0
15	16131.0
16	18502.0
17	20042.0
18	21101.0
19	22127.0
20	22909.0
21	24840.0
22	27180.0
23	29744.0
24	31814.0
25	34924.0
26	38528.0
27	43523.0
28	50352.0
29	59706.0
30	72217.0
31	89845.0
32	120207.0
33	170131.0
34	247745.0
35	392119.0
36	676671.0
37	2207351.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.86629920101156	20.057412129050263	13.74239102553064	23.33389764440754
2	15.732110319037778	19.59517278744	40.283312862556905	24.38940403096532
3	19.07334422332762	24.248819598411895	28.413806366062573	28.26402981219791
4	12.683148155850521	16.032957611552607	36.7398968587584	34.54399737383847
5	14.431641552211733	36.83022312423119	34.01628759257897	14.721847730978114
6	33.610458741523914	36.44781182421616	16.399893216091638	13.54183621816829
7	28.30430853582577	31.773467517967326	21.595844431795268	18.32637951441164
8	25.16932233060501	36.91810330372493	19.649841375594423	18.262732990075634
9	24.244927580298405	17.309006227843934	21.185557375934984	37.26050881592268
10	15.585376072530302	29.725918735191154	32.157553801460104	22.531151390818437
11	33.91787226517183	24.966190180541865	21.894722627572403	19.221214926713905
12	21.907063854446854	26.39884564814134	30.38025511015104	21.313835387260767
13	27.399019793938066	22.875119082672718	25.963007405092142	23.762853718297066
14	20.960257305119676	22.08612370445325	27.163678769242328	29.789940221184747
15	23.69418964893928	29.79903455562886	22.946150239706633	23.560625555725228
16	22.988751424181668	30.059131486174927	24.639751347271385	22.31236574237202
17	21.384665844055117	29.380535049266143	27.445840330338378	21.788958776340355
18	21.776069061701136	29.155012772891155	27.79547008397958	21.273448081428132
19	22.16881336727752	27.748789190206747	28.133310588613526	21.949086853902212
20	23.186856992233324	27.484698156222002	27.499185638076323	21.82925921346835
21	23.391166066590248	27.614874675357946	27.09318745632562	21.900771801726187
22	22.399824938088404	27.32236720220456	28.128873669918796	22.148934189788232
23	22.517514124293786	27.7417016277899	27.76586675616714	21.974917491749174
24	22.269627224974037	27.18821857872185	27.758643625197166	22.783510571106945
25	22.94262506302168	27.18330625735253	26.997008570948406	22.877060108677387
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1984.0
1	1984.0
2	2402.5
3	2821.0
4	2821.0
5	2821.0
6	4014.0
7	5207.0
8	5207.0
9	5207.0
10	8343.0
11	11479.0
12	11479.0
13	11479.0
14	19804.5
15	28130.0
16	28130.0
17	28130.0
18	46454.0
19	64778.0
20	64778.0
21	64778.0
22	95929.0
23	127080.0
24	127080.0
25	127080.0
26	170050.5
27	213021.0
28	213021.0
29	213021.0
30	262144.0
31	311267.0
32	311267.0
33	311267.0
34	358193.5
35	405120.0
36	405120.0
37	405120.0
38	446185.5
39	487251.0
40	487251.0
41	487251.0
42	499860.0
43	512469.0
44	512469.0
45	512469.0
46	524738.0
47	537007.0
48	537007.0
49	537007.0
50	518448.0
51	499889.0
52	499889.0
53	499889.0
54	465688.5
55	431488.0
56	431488.0
57	431488.0
58	384305.0
59	337122.0
60	337122.0
61	337122.0
62	290166.5
63	243211.0
64	243211.0
65	243211.0
66	192710.0
67	142209.0
68	142209.0
69	142209.0
70	106071.5
71	69934.0
72	69934.0
73	69934.0
74	49369.0
75	28804.0
76	28804.0
77	28804.0
78	18872.0
79	8940.0
80	8940.0
81	8940.0
82	5817.5
83	2695.0
84	2695.0
85	2695.0
86	1687.5
87	680.0
88	680.0
89	680.0
90	438.5
91	197.0
92	197.0
93	197.0
94	120.0
95	43.0
96	43.0
97	43.0
98	32.5
99	22.0
100	22.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02948904143400357
2	0.013839057352273094
3	0.019070623459594423
4	0.02061326474765071
5	0.019115337699827938
6	0.005902279710824066
7	1.3414272070054695E-4
8	4.0242816210164084E-4
9	7.154278437362504E-4
10	3.1299968163460954E-4
11	7.824992040865238E-4
12	7.824992040865238E-4
13	0.00257106881342715
14	5.812851230357034E-4
15	0.001989783690391446
16	0.0023251404921428138
17	0.00225806913179254
18	0.0021015692909752354
19	3.3535680175136737E-4
20	4.6949952245191426E-4
21	0.08260955883142015
22	0.07985963305705895
23	0.08044091818009466
24	9.613561650205864E-4
25	0.22576219893902053
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4472848.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.79162509413759
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.23618134454748	52.63424205755294
2	12.130892057872726	15.719604205422815
3	3.2966293631631434	6.4078192131717575
4	1.2691125011972604	3.2891144551942446
5	0.6062937431945015	1.9641378452989733
6	0.3401955960471912	1.3225095310659771
7	0.21582770282890054	0.9788679324633345
8	0.14366853061653803	0.7446814058825887
9	0.10567573133022808	0.6162212129307253
>10	0.5575624657362854	6.767094602544706
>50	0.05655040470868013	2.6016061321285178
>100	0.03862368361138421	4.577715682394934
>500	0.001915976967213655	0.8372853736821053
>1k	7.663903971376653E-4	1.0913641184403136
>5k	1.0450778142786227E-4	0.44773623182636796
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	7281	0.1627821915701137	No Hit
CCCATGTACTCTGCGTTGATACCAC	7041	0.15741648274209183	No Hit
GTATCAACGCAGAGTACTTTTTTTT	5515	0.1232995174439194	No Hit
ATTCCATTCCATTCCATTCCATTCC	4558	0.10190375349218217	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	2.2357120116757825E-5	0.0
7	0.0	0.0	0.0	2.2357120116757825E-5	0.0
8	0.0	0.0	0.0	2.2357120116757825E-5	0.0
9	0.0	0.0	0.0	8.94284804670313E-5	0.0
10	0.0	0.0	0.0	8.94284804670313E-5	0.0
11	0.0	0.0	0.0	8.94284804670313E-5	0.0
12	0.0	0.0	0.0	8.94284804670313E-5	0.0
13	0.0	0.0	0.0	1.1178560058378912E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTCGC	105	1.382432E-9	13.565455	10
TCGCGCG	85	3.967467E-6	12.288707	9
ACCGTCT	145	9.822543E-11	11.787781	8
CGTCCGC	135	4.771209E-9	11.254303	10
CGTACGG	60	0.0058817	11.082544	3
AAGCTCG	60	0.0058885575	11.08081	5
ATCGTCG	120	1.2851342E-7	11.078455	9
TATCGCG	95	1.3684961E-5	10.995036	7
TCGTCCG	130	3.870191E-7	10.226267	9
CGCCCAT	170	1.5734258E-9	10.054397	10
GTACCGC	180	3.9835868E-10	10.023589	6
GAACCGC	175	2.5975169E-9	9.767346	6
CCGTATA	195	1.6370905E-10	9.743223	2
TAGTCGC	90	0.0011166979	9.497837	5
CGCGCCT	150	2.668985E-7	9.495819	12
ACCGCCC	210	6.730261E-11	9.495712	8
CCGTACC	145	1.7115271E-6	9.171145	4
TACGGAG	135	6.8743593E-6	9.146064	5
GTATAGG	535	0.0	9.05776	1
CTGCGTA	105	4.51547E-4	9.043637	9
>>END_MODULE