FastQCFastQC Report
Wed 25 May 2016
SRR1294695_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294695_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4472848
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT158060.3533766405654742No Hit
GGTATCAACGCAGAGTACTTTTTTT113950.2547593837304554No Hit
TATCAACGCAGAGTACTTTTTTTTT105590.23606883131284587No Hit
GTACATGGGAAGCAGTGGTATCAAC77080.1723286818599693No Hit
CCCATGTACTCTGCGTTGATACCAC73060.16334111957303266No Hit
ACGCAGAGTACTTTTTTTTTTTTTT60360.13494757702475021No Hit
ATTCCATTCCATTCCATTCCATTCC48530.1084991039266257No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTCGC600.005907572311.07601110
GCGTAGG700.001465834910.88051
ACCGCCC1606.2500476E-910.0871948
ACCGGGC951.6562127E-49.9935058
GGACCGC1356.496084E-79.8468846
TAGACAG6650.09.5875045
CTAGGAC4700.09.5159573
TACGCAG800.0044561289.5159565
CGTCCGC1201.7154722E-59.49372310
CCTACAC5750.09.26771453
AACCGCC1451.7234543E-69.1664567
GCTCGTT2201.70985E-109.0621911
CGGTACT1901.029548E-89.0151174
AAACGGC2651.8189894E-128.95614217
TCGTCCG850.00747975068.9352689
GTACTGG10500.08.8857411
GTCCTAG5150.08.8734171
CTACACT7000.08.8362454
TTGCGTG1401.0956053E-58.81540316
CCCCTAG2409.822543E-118.7270671
Produced by FastQC (version 0.11.5)