FastQCFastQC Report
Wed 25 May 2016
SRR1294694_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294694_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2934401
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC113130.3855301303400592No Hit
CCCATGTACTCTGCGTTGATACCAC98750.3365252397337651No Hit
GAGTACATGGGAAGCAGTGGTATCA50780.1730506498600566No Hit
GTATCAACGCAGAGTACTTTTTTTT44790.152637625191649No Hit
CATGTACTCTGCGTTGATACCACTG34450.11740045072231096No Hit
GCGTTGATACCACTGCTTCCCATGT34130.11630993855304711No Hit
GTATCAACGCAGAGTACATGGGAAG32760.11164118332838628No Hit
ACGCAGAGTACATGGGAAGCAGTGG31770.10826741130472624No Hit
GGTATCAACGCAGAGTACTTTTTTT30780.10489363928106621No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGCGG400.0052940814.24451714
GCGTAAA400.0052940814.24451711
TAGGACG551.9612498E-413.8180464
CTAGGAC2750.013.1273673
GTACCGT701.0958138E-412.209176
GAACGAT600.005861063511.08757116
GGATTAG1900.011.00289251
CCGTTCA951.36704275E-510.9957689
ACCGTTC700.001496883510.8529668
TACCGTG803.786265E-410.6833887
TAGGACC1951.4551915E-1110.2306684
GTCTAGA3000.010.1359991
TTAACGC750.002648611310.1334073
ATTAGAC2250.010.1334063
ACACCGT1803.9835868E-1010.0235786
GCCCTAG2002.5465852E-119.9776221
TGTGCGA1356.482205E-79.84806210
GTCTAGT2201.6370905E-119.5024981
CTAGCAC3000.09.5000693
TATACTG6050.09.419955
Produced by FastQC (version 0.11.5)