##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294694_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2934401
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.966197871388403	33.0	33.0	33.0	27.0	33.0
2	30.982943026532503	33.0	33.0	33.0	27.0	33.0
3	30.94351453669761	33.0	33.0	33.0	27.0	33.0
4	34.43678420229546	37.0	37.0	37.0	27.0	37.0
5	34.690813559564624	37.0	37.0	37.0	27.0	37.0
6	34.80005050434484	37.0	37.0	37.0	27.0	37.0
7	34.798748364657726	37.0	37.0	37.0	27.0	37.0
8	34.70565951960894	37.0	37.0	37.0	27.0	37.0
9	34.45031950302634	37.0	37.0	37.0	27.0	37.0
10	34.55068615366475	37.0	37.0	37.0	27.0	37.0
11	34.73427183265	37.0	37.0	37.0	27.0	37.0
12	34.67607596916713	37.0	37.0	37.0	27.0	37.0
13	34.58525436707526	37.0	37.0	37.0	27.0	37.0
14	35.7358367176129	40.0	37.0	40.0	27.0	40.0
15	36.03189679938086	40.0	37.0	40.0	27.0	40.0
16	36.04433409067131	40.0	37.0	40.0	27.0	40.0
17	35.99972055625663	40.0	37.0	40.0	27.0	40.0
18	36.00488208666777	40.0	37.0	40.0	27.0	40.0
19	36.092346274418524	40.0	37.0	40.0	27.0	40.0
20	35.98403456105692	40.0	37.0	40.0	27.0	40.0
21	35.93585505184874	40.0	37.0	40.0	27.0	40.0
22	35.80789196841195	40.0	37.0	40.0	27.0	40.0
23	35.41889026073805	37.0	33.0	40.0	27.0	40.0
24	35.731890426700375	37.0	37.0	40.0	27.0	40.0
25	34.557756761942215	37.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.5896722452904584
1101	2	0.7094498382525991
1101	3	0.5729245911138712
1101	4	0.6460175599241325
1101	5	0.7020530150569542
1101	6	0.5111620892772848
1101	7	0.6141426393214005
1101	8	0.5789662433503437
1101	9	0.9549322951098844
1101	10	0.8600196495824335
1101	11	0.5999687124117656
1101	12	0.650894280667913
1101	13	0.6721893303281306
1101	14	1.3618774532090825
1101	15	1.059053701635051
1101	16	1.0830010675688797
1101	17	1.0193009687712973
1101	18	0.9669271236190085
1101	19	0.9998087494478796
1101	20	1.1646405995475604
1101	21	1.1474317032814199
1101	22	1.1646821994510432
1101	23	1.6190195275285788
1101	24	1.2701614620600026
1101	25	1.1079877583467805
1102	1	0.3331675234659208
1102	2	0.4097028070419739
1102	3	0.262975298813096
1102	4	0.25420601208215743
1102	5	0.3234979262178328
1102	6	0.3058260543945224
1102	7	0.2605482074832963
1102	8	0.3569431652893158
1102	9	0.5639664769454669
1102	10	0.6230391105752773
1102	11	0.3956354088930851
1102	12	0.32648369452891757
1102	13	0.4302272279810708
1102	14	1.000508660567725
1102	15	0.7982040162935817
1102	16	0.6397244903214911
1102	17	0.7747602312251374
1102	18	0.8143491625728956
1102	19	0.6937115768455371
1102	20	0.7241172726196297
1102	21	0.7149221089449114
1102	22	0.8167268200234972
1102	23	1.099593144370786
1102	24	0.8861473006692933
1102	25	0.7644891473867972
1103	1	0.46053236923807717
1103	2	0.6062932922747599
1103	3	0.39682573906040375
1103	4	0.30788340518513735
1103	5	0.5360848171337054
1103	6	0.43332653476706184
1103	7	0.4001659439678775
1103	8	0.6385488529002217
1103	9	0.7419699356277789
1103	10	0.6990470451994
1103	11	0.6444028294620665
1103	12	0.6053619963837207
1103	13	0.4902189768762142
1103	14	1.2658986665582503
1103	15	0.8851440997088602
1103	16	0.7179150117234983
1103	17	0.7996599181352622
1103	18	0.8825417184939113
1103	19	0.864020597676749
1103	20	0.9926313955723955
1103	21	1.0328609151343713
1103	22	1.0158225893542507
1103	23	1.4108361224544907
1103	24	1.102456231077447
1103	25	1.0528324837719083
1104	1	0.22734084166452817
1104	2	0.41971376693118856
1104	3	0.31804272432233205
1104	4	0.22107134084498625
1104	5	0.19929782135794483
1104	6	0.09869618384458079
1104	7	0.10361474236049162
1104	8	0.2955108048059927
1104	9	0.4301076406194184
1104	10	0.4646284675223953
1104	11	0.25765081742915896
1104	12	0.2656706016361454
1104	13	0.34902926770897835
1104	14	0.6914051825142025
1104	15	0.5171360777682565
1104	16	0.4502229659415633
1104	17	0.4884699844734186
1104	18	0.51243317535166
1104	19	0.5108615844053759
1104	20	0.6007479980710215
1104	21	0.7068919402889904
1104	22	0.7890899586890399
1104	23	1.0289197242459593
1104	24	0.8451928951624197
1104	25	0.7615782324052986
1105	1	0.10401304758114094
1105	2	0.2405380994553461
1105	3	0.2934379953056876
1105	4	0.21925796334868863
1105	5	0.15920924622143673
1105	6	0.13205166284998882
1105	7	0.08486549518119091
1105	8	0.2015775673050868
1105	9	-1.6490702168802613
1105	10	-0.2771613030272988
1105	11	-0.02288340112691145
1105	12	0.06778703312068757
1105	13	0.09708581034855257
1105	14	-1.707295264892565
1105	15	-0.36311039808691703
1105	16	-0.18425544778641267
1105	17	0.10601243412080663
1105	18	0.031084154651026097
1105	19	0.07785951105694267
1105	20	0.25521132328994156
1105	21	0.4552764201186861
1105	22	0.4509848277275026
1105	23	0.8667497806617277
1105	24	0.5506273260375281
1105	25	0.1484756784976753
1106	1	0.04568413439872998
1106	2	0.002612292392068838
1106	3	0.08995433414368392
1106	4	-1.0641619195478853
1106	5	-0.27947700147120713
1106	6	-0.14583347300244753
1106	7	-0.07179658927853438
1106	8	0.14411444087362923
1106	9	0.3013115269551534
1106	10	0.3111895080970868
1106	11	0.2549320603378149
1106	12	0.16108249386343942
1106	13	0.16687004681915596
1106	14	0.5391991333060702
1106	15	0.29237013296776837
1106	16	0.18830864149074245
1106	17	0.09958404997854586
1106	18	0.2048916697421319
1106	19	0.2352875285838394
1106	20	0.31618456386123484
1106	21	0.5539338004898013
1106	22	0.5771730040809473
1106	23	0.7075951386493173
1106	24	0.4670868618120281
1106	25	0.3259641943536451
1107	1	0.4034135621192725
1107	2	0.3648791508606166
1107	3	0.34624853848320214
1107	4	0.5077391050408337
1107	5	0.40573368678256827
1107	6	0.2246685261090562
1107	7	0.11288840669033817
1107	8	0.2901121044634749
1107	9	0.4613783050361491
1107	10	0.4382966844490426
1107	11	0.3487077837670256
1107	12	0.29389013491186233
1107	13	0.2443796310368711
1107	14	0.7329260835856672
1107	15	0.4819213964861575
1107	16	0.4780400718485609
1107	17	0.56226213073748
1107	18	0.4670775530297462
1107	19	0.38520866886481997
1107	20	0.5325303854713894
1107	21	0.5520047342781922
1107	22	0.5106518341834985
1107	23	0.7638664254847924
1107	24	0.7166216952429068
1107	25	0.6610575919970145
1108	1	0.5360676447733539
1108	2	0.40496924095070597
1108	3	0.6000237422584043
1108	4	0.7683568656585962
1108	5	0.5764830075318912
1108	6	0.4325192339597663
1108	7	0.39875999256192074
1108	8	0.4333696477210154
1108	9	0.30099649465433487
1108	10	0.48563688178924025
1108	11	0.5004380854973292
1108	12	0.46894375996548376
1108	13	0.515908302565542
1108	14	-0.8303514796918989
1108	15	0.2078616224263854
1108	16	0.49143203524052126
1108	17	0.5891318576072067
1108	18	0.7426817286339187
1108	19	0.777157760813914
1108	20	0.8233712263122257
1108	21	0.93916332143678
1108	22	0.8755023990340618
1108	23	1.121438283056655
1108	24	0.9838961125173284
1108	25	1.0118941428335688
1109	1	0.36768923308174095
1109	2	0.28878248843953713
1109	3	0.3547171142943135
1109	4	0.34659512487183264
1109	5	0.43809076344508213
1109	6	0.2872224519071551
1109	7	0.19182299269998992
1109	8	0.32876451874142987
1109	9	0.43489543118485585
1109	10	0.40767984179596084
1109	11	0.25985222045071765
1109	12	0.23862697701711966
1109	13	0.23442692595905612
1109	14	0.6009910547567614
1109	15	0.18296932596376791
1109	16	0.2496756726903726
1109	17	0.2581642265964348
1109	18	0.3088306151020461
1109	19	0.2695771492643644
1109	20	0.49170975465938227
1109	21	0.45430853567604146
1109	22	0.5954953181210314
1109	23	0.931971113434173
1109	24	0.7020616156267465
1109	25	0.709896903390252
1110	1	0.3222678559735641
1110	2	0.2606361716176373
1110	3	0.31038162761629096
1110	4	0.44864264594437486
1110	5	0.36762827367715545
1110	6	0.15607878251931595
1110	7	0.05427017307210491
1110	8	0.12971324406461093
1110	9	0.3202305638884084
1110	10	0.29751524366759696
1110	11	0.14938950944875273
1110	12	0.1039328699545976
1110	13	0.09017735183459052
1110	14	0.4058198564794395
1110	15	0.27498203954680633
1110	16	0.1873400311485156
1110	17	0.23739440586975036
1110	18	0.29739908335127296
1110	19	0.19700150565765284
1110	20	0.29342782136882306
1110	21	0.4057491380225926
1110	22	0.5076196811513469
1110	23	0.6681399297582971
1110	24	0.5538215324427469
1110	25	0.5340429399823634
1111	1	0.04325439780333795
1111	2	-0.013048925285986002
1111	3	0.21704044122284927
1111	4	0.3342430313072313
1111	5	0.22205565547983497
1111	6	0.21603108279227712
1111	7	0.1172943025451687
1111	8	0.17827080948678997
1111	9	0.1909473240350934
1111	10	0.02930020454994775
1111	11	-0.01290043734238111
1111	12	0.03078281467865196
1111	13	0.06102750326668627
1111	14	-1.852577750136696
1111	15	-0.6848880850501615
1111	16	-0.18925046729471262
1111	17	0.06126494019159168
1111	18	0.11760202635706918
1111	19	0.11035996693773598
1111	20	0.10977672055620502
1111	21	0.21804153024373107
1111	22	0.09513848327822672
1111	23	-1.9469591701389035
1111	24	-0.442371071469573
1111	25	-0.06696268542943784
1112	1	-0.1652252065194979
1112	2	-0.23872835274688597
1112	3	0.013492405727070889
1112	4	0.12390072120245321
1112	5	-0.7678112867624023
1112	6	-0.9114786600381279
1112	7	-1.037929294127359
1112	8	-0.7887238743725078
1112	9	-0.6242638306059902
1112	10	-0.9531174569651029
1112	11	-0.8891469540877139
1112	12	-0.8240752330535059
1112	13	-0.876447689790453
1112	14	-0.6105082598486788
1112	15	-0.9038169392521738
1112	16	-0.9123880185795343
1112	17	-0.8317253632500154
1112	18	-1.0161595802073862
1112	19	-0.8292694456132921
1112	20	-1.0660266130856115
1112	21	-1.8398663575929035
1112	22	-1.673571350039687
1112	23	-1.2740123623939965
1112	24	-1.4423489637277527
1112	25	-1.8670809361415124
1113	1	-0.03585835660049952
1113	2	-0.32108832406391485
1113	3	-0.17564259015639294
1113	4	0.014580082740707212
1113	5	-0.09591528909952984
1113	6	-0.23041473418892622
1113	7	-0.1552519510205741
1113	8	0.04785222846126658
1113	9	0.09935181754953959
1113	10	-0.3265392294666256
1113	11	-0.27798582482842704
1113	12	-0.2415612235456308
1113	13	-0.2858586265020051
1113	14	-0.23973435073121152
1113	15	-0.2993458479712672
1113	16	-0.07390104542998444
1113	17	-0.15754667894863417
1113	18	-0.2169244594998716
1113	19	-0.28520389970725546
1113	20	-0.8144556891220489
1113	21	-0.7061638637677063
1113	22	-0.2531891759765301
1113	23	-0.4654645707173657
1113	24	-0.21167544501741986
1113	25	-1.3960122297967317
1114	1	-0.7177254747263966
1114	2	-0.5617940010971978
1114	3	-0.47770823333023316
1114	4	-1.4256218705170554
1114	5	-0.6521078453054443
1114	6	-0.51143328233033
1114	7	-0.35572561925791746
1114	8	-0.16688226431924136
1114	9	-0.29868878051653525
1114	10	-0.3502280712893793
1114	11	-0.4703758056112761
1114	12	-0.4428926290979618
1114	13	-0.3685647265826404
1114	14	-0.36332733182381105
1114	15	-1.1296220673680892
1114	16	-0.6483244086016526
1114	17	-0.586130190394293
1114	18	-0.5465833761033849
1114	19	-0.6274337506094838
1114	20	-1.748473558861427
1114	21	-6.5512705149448145
1114	22	-7.673758659938986
1114	23	-7.702867220418309
1114	24	-5.794488529899468
1114	25	-7.113544519443675
1115	1	-1.0746843221056572
1115	2	-1.0492972694902534
1115	3	-0.9108975165889
1115	4	-0.734536014693397
1115	5	-0.8640770459696157
1115	6	-1.0092731662351042
1115	7	-1.1572818109318845
1115	8	-0.7986810973659928
1115	9	-2.673745304334915
1115	10	-1.7556353388619854
1115	11	-1.2741142069502018
1115	12	-1.1718718940049442
1115	13	-1.5810415237040303
1115	14	-1.2501568706989943
1115	15	-2.1179493068450768
1115	16	-1.3399958101716791
1115	17	-3.068525901733416
1115	18	-2.7391755900330708
1115	19	-2.196438477892542
1115	20	-1.8967327363847701
1115	21	-1.3818893026497392
1115	22	-1.030463126983605
1115	23	-0.44119796576115533
1115	24	-1.521965961251837
1115	25	-1.2977602276171964
1116	1	-1.5552150012621446
1116	2	-0.776761865495228
1116	3	-0.6302889713242692
1116	4	-0.3094737623621455
1116	5	-0.30745459655780394
1116	6	-0.24681436362478593
1116	7	-0.5809716130251914
1116	8	-1.7255335792430486
1116	9	-0.7493918078252193
1116	10	-2.219229418514324
1116	11	-1.022593039054712
1116	12	-0.64546347725544
1116	13	-0.57747644557206
1116	14	-1.0295641089425516
1116	15	-1.1195371505997613
1116	16	-0.8176939293987502
1116	17	-0.7260614454188001
1116	18	-1.3301300837216203
1116	19	-0.7793114188491828
1116	20	-1.1928392807728017
1116	21	-0.5471301964156723
1116	22	-0.1613299792431846
1116	23	0.0037903798574205894
1116	24	-0.13376255647214208
1116	25	0.04409217465137516
1201	1	0.22424446707544732
1201	2	0.30032945769321273
1201	3	0.2594046288531153
1201	4	0.48278452997826804
1201	5	0.4004341107486553
1201	6	0.31235751099026743
1201	7	0.41424829789904294
1201	8	0.45599483384512496
1201	9	0.7163493359251021
1201	10	0.6773707013848309
1201	11	0.5533422701509707
1201	12	0.4950780398794592
1201	13	0.46375408108086447
1201	14	1.0830272183520293
1201	15	0.8607539470249748
1201	16	0.837494172663348
1201	17	0.6895662321236529
1201	18	0.6726976748485711
1201	19	0.7170929752631636
1201	20	0.8732911246122583
1201	21	0.8845929843610421
1201	22	0.9270756631343247
1201	23	0.6768973830081961
1201	24	0.7972146763758445
1201	25	0.8022588673235589
1202	1	-0.36299336845576846
1202	2	-0.042483782758814925
1202	3	-0.03551743769351745
1202	4	-0.02186461975352927
1202	5	0.12638488390690839
1202	6	-0.008867002487129128
1202	7	0.08424728723466046
1202	8	0.27076073402891154
1202	9	0.3211833998065785
1202	10	0.5196589578390416
1202	11	0.3141264334144438
1202	12	0.21737367044045897
1202	13	0.3232373882741726
1202	14	0.5022240396253892
1202	15	0.4803716912969378
1202	16	0.3907788319010095
1202	17	0.3230770555905309
1202	18	0.29034743097899707
1202	19	0.23787457443366833
1202	20	0.4990911403337037
1202	21	0.5027032478449911
1202	22	0.5650338777769832
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2211	2	0.3967115211867096
2211	3	0.4472115457223609
2211	4	0.49275271997076686
2211	5	0.4477993926558099
2211	6	0.28303672694587334
2211	7	0.2571158977797552
2211	8	0.40076606386220703
2211	9	0.48967411429430285
2211	10	0.5070421184664369
2211	11	0.35261073034779855
2211	12	0.4072792562507743
2211	13	0.26563331149557
2211	14	0.7102256784584853
2211	15	0.5065201434698423
2211	16	0.29331757290011495
2211	17	0.4844422905745418
2211	18	0.43667419689417386
2211	19	0.40891635742606525
2211	20	0.4820189954202476
2211	21	0.49234034783556524
2211	22	0.6676275486905965
2211	23	0.7677018508683346
2211	24	0.6896331406392875
2211	25	0.6765786121205508
2212	1	-0.3476313206189765
2212	2	-0.4275021647472421
2212	3	-0.4092398200944025
2212	4	-0.7227356325277228
2212	5	-0.4306041384568573
2212	6	-0.8329973777811048
2212	7	-0.5803906847580862
2212	8	-0.4384242121735298
2212	9	-0.34302218895244607
2212	10	-0.800258035684628
2212	11	-0.7640045526295225
2212	12	-0.7059954486121711
2212	13	-0.9170323530295761
2212	14	-0.7065566812975561
2212	15	-0.8280915586068858
2212	16	-0.7407321001272749
2212	17	-0.8851583528386158
2212	18	-0.6583168508543622
2212	19	-0.8334914631076273
2212	20	-0.75207424203942
2212	21	-0.7678226118649434
2212	22	-0.9325854861705238
2212	23	-0.3224798750491473
2212	24	-0.5198069367234837
2212	25	-1.2430722979452327
2213	1	-0.17133903804316475
2213	2	-1.2866632047800977
2213	3	-0.22593346894470656
2213	4	0.17705521697990179
2213	5	0.03919673254070233
2213	6	0.05072771282397781
2213	7	0.060193621208668446
2213	8	0.1391165911728791
2213	9	0.14467378915130524
2213	10	-0.42000412221242556
2213	11	-0.1222141601057416
2213	12	-0.007397921294227672
2213	13	-0.35502266672608584
2213	14	0.017303818783076963
2213	15	-0.10819916904424076
2213	16	-0.012888337932309923
2213	17	0.15232119128834398
2213	18	0.08478783303510795
2213	19	0.18562658731552517
2213	20	0.20351068022217333
2213	21	-0.46234879319008826
2213	22	-0.7882686745730751
2213	23	-0.02362204233153875
2213	24	0.04802432418652103
2213	25	-0.4827008353679645
2214	1	-0.3654179933682329
2214	2	-0.6445655487770097
2214	3	-0.582310130429164
2214	4	-0.8832066408888295
2214	5	-0.5696563334627527
2214	6	-0.3734022134407837
2214	7	-0.6656634570705293
2214	8	-0.24344750015722383
2214	9	-0.22486933442692703
2214	10	-2.051140869779509
2214	11	-1.0025954027451647
2214	12	-0.6957668854525565
2214	13	-0.4067576799075141
2214	14	-1.1226600951516374
2214	15	-0.7118910195127484
2214	16	-0.8300095717316296
2214	17	-0.658818527320669
2214	18	-0.5988303392447065
2214	19	-1.6002317095016068
2214	20	-2.6783127727994227
2214	21	-2.0307322976549855
2214	22	-2.2415283662282164
2214	23	-2.670324356937499
2214	24	-1.71945326350086
2214	25	-2.108947001480246
2215	1	-0.11569930793193706
2215	2	-0.19937677492345784
2215	3	-0.7667264817304229
2215	4	-0.2618681122875586
2215	5	-0.14248893261967055
2215	6	-0.04990698774259528
2215	7	-0.15570954783255075
2215	8	-0.22931496335501578
2215	9	-0.1940495855123885
2215	10	-0.18768101672973359
2215	11	-0.4749024407815412
2215	12	-0.3938221081623112
2215	13	-0.22098130630798352
2215	14	-0.0945559369258433
2215	15	-0.5108477993661467
2215	16	-0.26000764649406705
2215	17	-0.07157193312339416
2215	18	-0.12583136508024495
2215	19	-1.9776776950939308
2215	20	-2.2018604396701065
2215	21	-3.192447511916683
2215	22	-2.3912964756736557
2215	23	-3.6478851384115423
2215	24	-2.464106562697147
2215	25	-1.6398296798017853
2216	1	-1.4804527011800452
2216	2	-1.1788463168093628
2216	3	-0.7742628828122946
2216	4	-0.7301455140259279
2216	5	-1.8272489339563833
2216	6	-1.5758139829379871
2216	7	-0.9828404947080429
2216	8	-5.063214080587681
2216	9	-4.001308619351622
2216	10	-2.1467503218886748
2216	11	-0.9935963245805084
2216	12	-1.0126840508541264
2216	13	-1.8925814555020253
2216	14	-3.5091307069632833
2216	15	-1.8154579922080671
2216	16	-2.7571023512914508
2216	17	-2.6719072963149415
2216	18	-2.5241045303115683
2216	19	-1.5491139356628523
2216	20	-0.7182469902155191
2216	21	-0.5504141933518341
2216	22	-0.5532563614496695
2216	23	-0.736696501736759
2216	24	-0.29205548961039085
2216	25	-0.08337919357366275
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	42.0
8	225.0
9	722.0
10	1537.0
11	2930.0
12	5375.0
13	7876.0
14	10526.0
15	13604.0
16	15580.0
17	16556.0
18	17400.0
19	17682.0
20	18175.0
21	19566.0
22	20690.0
23	21619.0
24	23161.0
25	25014.0
26	26967.0
27	30017.0
28	34438.0
29	40609.0
30	48796.0
31	60290.0
32	79634.0
33	112027.0
34	160634.0
35	251271.0
36	433955.0
37	1417481.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.482963861910974	20.46187620804031	13.750984329139209	23.304175600909502
2	15.521943843991851	20.026019991151703	40.32600997035343	24.126026194503012
3	19.21929373956301	24.906831938212694	28.404115381482008	27.469758940742285
4	12.719235005199977	16.582045610493626	36.480657143431756	34.218062240874644
5	13.989049356974398	37.40326664982791	34.328558149427906	14.27912584376978
6	32.937092036470105	36.801330770942656	16.49920132613601	13.762375866451231
7	27.64757080522412	32.171342031115174	21.848405868617238	18.332681295043464
8	24.718714771189084	37.516272558366516	19.93393511696476	17.831077553479634
9	24.204679484579895	17.83240292763419	21.350212293350936	36.612705294434974
10	15.297833012313266	30.513161822739672	32.248783054502525	21.940222110444534
11	33.32967555008175	25.74923782929421	21.691483930858354	19.22960268976568
12	21.53607811423853	27.18376591355757	30.470435040719146	20.809720931484758
13	27.03914818265653	23.428316710617523	26.009409958907252	23.52312514781869
14	20.831247433448578	23.014566936513102	27.1224805197682	29.031705110270124
15	23.654481924976306	30.272533672669923	23.060926889164325	23.01205751318945
16	22.31124494433486	30.726448131976884	25.256992051342518	21.70531487234573
17	21.13133293165355	29.976069608743895	27.52823118216542	21.36436627743714
18	21.395129937403215	29.333007535606008	28.342800551811383	20.929061975179394
19	21.872204488086282	28.476607241502585	28.636027032525657	21.015161237885476
20	22.826390330129602	27.975155338770236	27.903113182181116	21.295341148919043
21	22.80785699304098	28.192410727227152	27.66686049386716	21.33287178586471
22	22.017932188349704	27.9448781813674	28.02505500006991	22.012134630212984
23	21.78091334363491	28.193491863038357	28.25276401600553	21.772830777321207
24	22.00472741375162	27.93566873207028	27.97577950186309	22.08382435231501
25	22.51166809831771	27.45320379595301	27.30206926230978	22.7330588434195
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2549.0
1	2549.0
2	2786.0
3	3023.0
4	3023.0
5	3023.0
6	4129.5
7	5236.0
8	5236.0
9	5236.0
10	7709.5
11	10183.0
12	10183.0
13	10183.0
14	16036.0
15	21889.0
16	21889.0
17	21889.0
18	34730.0
19	47571.0
20	47571.0
21	47571.0
22	68908.5
23	90246.0
24	90246.0
25	90246.0
26	118870.0
27	147494.0
28	147494.0
29	147494.0
30	180634.5
31	213775.0
32	213775.0
33	213775.0
34	243839.5
35	273904.0
36	273904.0
37	273904.0
38	298441.5
39	322979.0
40	322979.0
41	322979.0
42	330606.0
43	338233.0
44	338233.0
45	338233.0
46	354522.5
47	370812.0
48	370812.0
49	370812.0
50	350660.5
51	330509.0
52	330509.0
53	330509.0
54	298865.5
55	267222.0
56	267222.0
57	267222.0
58	234200.5
59	201179.0
60	201179.0
61	201179.0
62	170806.0
63	140433.0
64	140433.0
65	140433.0
66	110341.0
67	80249.0
68	80249.0
69	80249.0
70	60169.0
71	40089.0
72	40089.0
73	40089.0
74	28752.0
75	17415.0
76	17415.0
77	17415.0
78	11928.5
79	6442.0
80	6442.0
81	6442.0
82	4307.5
83	2173.0
84	2173.0
85	2173.0
86	1383.0
87	593.0
88	593.0
89	593.0
90	376.5
91	160.0
92	160.0
93	160.0
94	94.0
95	28.0
96	28.0
97	28.0
98	21.5
99	15.0
100	15.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03172708842452003
2	0.017141488160616086
3	0.02467283782959452
4	0.025013622882489477
5	0.023582325660330677
6	0.005520717856898222
7	4.430205687634376E-4
8	4.430205687634376E-4
9	6.134130952109136E-4
10	1.70392526447476E-4
11	6.474916005004088E-4
12	0.0010905121692638465
13	0.0021469458332381976
14	5.452560846319233E-4
15	0.0016357682538957695
16	0.0023514168649751686
17	0.001942474801501226
18	0.002215102843817188
19	3.40785052894952E-4
20	2.726280423159616E-4
21	0.07619953782731126
22	0.07265537327720376
23	0.07374588544646761
24	9.201196428163703E-4
25	0.2230778956250356
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2934401.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.94752029578338
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.9232059521062	66.01601948222145
2	9.207762783998223	13.986135018329279
3	2.025715783995023	4.615442718553522
4	0.7084341482662169	2.152152674146981
5	0.34370461575581224	1.3051756640434489
6	0.19383213831649934	0.8832642155260455
7	0.1226643550034477	0.6521237513835466
8	0.08577501905506306	0.5211520000444486
9	0.05682837475812956	0.3884376730787486
>10	0.30324492352016325	4.076848566936058
>50	0.018282295244802606	0.9652639727833185
>100	0.00816369569998242	1.0755509311330265
>500	0.001080414013916047	0.5810161169097933
>1k	0.0011704485147991702	1.8833961884351282
>5k	9.003450113839772E-5	0.5112353768705951
>10k+	4.501725056919886E-5	0.38678564960456374
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	11313	0.3855301303400592	No Hit
CCCATGTACTCTGCGTTGATACCAC	9875	0.3365252397337651	No Hit
GAGTACATGGGAAGCAGTGGTATCA	5078	0.1730506498600566	No Hit
GTATCAACGCAGAGTACTTTTTTTT	4479	0.152637625191649	No Hit
CATGTACTCTGCGTTGATACCACTG	3445	0.11740045072231096	No Hit
GCGTTGATACCACTGCTTCCCATGT	3413	0.11630993855304711	No Hit
GTATCAACGCAGAGTACATGGGAAG	3276	0.11164118332838628	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	3177	0.10826741130472624	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3078	0.10489363928106621	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.4078505289495197E-5	0.0
5	3.4078505289495197E-5	0.0	0.0	3.4078505289495197E-5	0.0
6	3.4078505289495197E-5	0.0	0.0	3.4078505289495197E-5	0.0
7	3.4078505289495197E-5	0.0	0.0	3.4078505289495197E-5	0.0
8	3.4078505289495197E-5	0.0	0.0	3.4078505289495197E-5	0.0
9	3.4078505289495197E-5	0.0	0.0	3.4078505289495197E-5	0.0
10	3.4078505289495197E-5	0.0	0.0	6.815701057899039E-5	0.0
11	3.4078505289495197E-5	0.0	0.0	1.022355158684856E-4	0.0
12	6.815701057899039E-5	3.4078505289495197E-5	0.0	1.022355158684856E-4	0.0
13	6.815701057899039E-5	3.4078505289495197E-5	0.0	1.3631402115798079E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCGCGG	40	0.00529408	14.244517	14
GCGTAAA	40	0.00529408	14.244517	11
TAGGACG	55	1.9612498E-4	13.818046	4
CTAGGAC	275	0.0	13.127367	3
GTACCGT	70	1.0958138E-4	12.20917	6
GAACGAT	60	0.0058610635	11.087571	16
GGATTAG	190	0.0	11.0028925	1
CCGTTCA	95	1.36704275E-5	10.995768	9
ACCGTTC	70	0.0014968835	10.852966	8
TACCGTG	80	3.786265E-4	10.683388	7
TAGGACC	195	1.4551915E-11	10.230668	4
GTCTAGA	300	0.0	10.135999	1
TTAACGC	75	0.0026486113	10.133407	3
ATTAGAC	225	0.0	10.133406	3
ACACCGT	180	3.9835868E-10	10.023578	6
GCCCTAG	200	2.5465852E-11	9.977622	1
TGTGCGA	135	6.482205E-7	9.848062	10
GTCTAGT	220	1.6370905E-11	9.502498	1
CTAGCAC	300	0.0	9.500069	3
TATACTG	605	0.0	9.41995	5
>>END_MODULE