FastQCFastQC Report
Wed 25 May 2016
SRR1294694_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294694_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2934401
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT126430.43085454237508775No Hit
GTACATGGGAAGCAGTGGTATCAAC112470.3832809489909525No Hit
CCCATGTACTCTGCGTTGATACCAC107780.36729813001017925No Hit
GGTATCAACGCAGAGTACTTTTTTT95870.32671063021039043No Hit
TATCAACGCAGAGTACTTTTTTTTT85810.29242765388915826No Hit
GAGTACATGGGAAGCAGTGGTATCA57280.1952016782982285No Hit
ACGCAGAGTACTTTTTTTTTTTTTT48620.16568969271752568No Hit
CATGTACTCTGCGTTGATACCACTG40370.1375749258536921No Hit
GCGTTGATACCACTGCTTCCCATGT38750.1320542079967939No Hit
ACGCAGAGTACATGGGAAGCAGTGG38470.13110000984868803No Hit
GTATCAACGCAGAGTACATGGGAAG35550.12114908630415543No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGTA604.114449E-412.6591699
GCGACGT550.003083202312.0837548
TAGACAG4400.011.027965
TGTTCCG1002.3608009E-510.4657495
CCCGGAC1002.3608009E-510.4657493
GTCTAGG3400.010.35931
CGAATGT1106.0928614E-610.35750315
GTCCTAG2950.010.3260811
GGTCTAG1502.3712346E-810.1539791
GTGCTAG3250.09.9587111
AATCCGA1252.5914378E-69.87415211
CTAGGAC2800.09.8541153
TTAGACA2650.09.6938334
CTAGACA3250.09.6606914
GTGTAGG3700.09.5193561
GGCTAGA2750.09.3462771
CCTACAC3600.09.2500313
TAGGACT3500.09.242484
ATCCGAA1356.9032176E-69.14273312
TATTCCG1252.7071203E-59.1337455
Produced by FastQC (version 0.11.5)