FastQCFastQC Report
Wed 25 May 2016
SRR1294693_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294693_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2631359
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC138620.5268000299465029No Hit
CCCATGTACTCTGCGTTGATACCAC121160.4604464841171425No Hit
GAGTACATGGGAAGCAGTGGTATCA62480.2374438455566116No Hit
CATGTACTCTGCGTTGATACCACTG40910.1554709942657007No Hit
GCGTTGATACCACTGCTTCCCATGT40650.15448291168175834No Hit
GTATCAACGCAGAGTACATGGGAAG39710.15091061310904366No Hit
GTATCAACGCAGAGTACTTTTTTTT38700.1470722923021906No Hit
ACGCAGAGTACATGGGAAGCAGTGG38390.14589419383672086No Hit
TATCAACGCAGAGTACATGGGAAGC29340.11150131928026545No Hit
GGTATCAACGCAGAGTACATGGGAA28550.10849906835213288No Hit
GCTTCCCATGTACTCTGCGTTGATA27580.10481276025050172No Hit
GTGGTATCAACGCAGAGTACATGGG27510.10454673801636341No Hit
GGTATCAACGCAGAGTACTTTTTTT27270.103634661785032No Hit
ACTCTGCGTTGATACCACTGCTTCC26590.10105044579625964No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGTC700.001496223710.85346112
AGGGTCG856.610996E-410.0563695
CGGGACT1054.1164574E-59.9503314
AGACCGT1054.1250616E-59.948446
TTAGGAC2201.8189894E-129.929963
GAGACCG900.00111654379.4976825
TATCGGT1606.6747816E-89.496778513
ATCGGTC1502.6637645E-79.49659914
TATACTG5800.09.3339285
ACTATCG1451.7150633E-69.16930411
GTCTCGC1356.8404224E-69.14921951
CCGTCCC1151.11540794E-49.0837039
AGGACCG1054.5050596E-49.0454115
GCACCGT1054.5119104E-49.0440366
CTCGTCT950.00182997028.99694813
GTGTAGA3500.08.9581921
AACACCG850.00745252158.9389945
ACTGATC2451.6370905E-118.91517458
GTATTAG4200.08.8224611
TAGGACA3350.08.7892334
Produced by FastQC (version 0.11.5)