##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294693_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2631359 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.765932356626365 33.0 33.0 33.0 27.0 33.0 2 30.762271890684623 33.0 33.0 33.0 27.0 33.0 3 30.676311366103977 33.0 33.0 33.0 27.0 33.0 4 34.09605530830267 37.0 33.0 37.0 27.0 37.0 5 34.30729292354255 37.0 37.0 37.0 27.0 37.0 6 34.54696641545301 37.0 37.0 37.0 27.0 37.0 7 34.47991627140196 37.0 37.0 37.0 27.0 37.0 8 34.40985817594635 37.0 37.0 37.0 27.0 37.0 9 34.147847937130585 37.0 37.0 37.0 27.0 37.0 10 34.24054756496548 37.0 37.0 37.0 27.0 37.0 11 34.47502906292908 37.0 37.0 37.0 27.0 37.0 12 34.40511879982929 37.0 37.0 37.0 27.0 37.0 13 34.31151241620775 37.0 37.0 37.0 27.0 37.0 14 35.37805179756924 37.0 37.0 40.0 27.0 40.0 15 35.698321285693055 37.0 37.0 40.0 27.0 40.0 16 35.69472010470635 37.0 37.0 40.0 27.0 40.0 17 35.63579541978118 37.0 37.0 40.0 27.0 40.0 18 35.62522939667297 37.0 37.0 40.0 27.0 40.0 19 35.70610585632747 37.0 37.0 40.0 27.0 40.0 20 35.634988612348224 37.0 37.0 40.0 27.0 40.0 21 35.572079674419186 37.0 37.0 40.0 27.0 40.0 22 35.42547938156671 37.0 33.0 40.0 27.0 40.0 23 35.02989481860894 37.0 33.0 40.0 22.0 40.0 24 35.36478108840337 37.0 33.0 40.0 27.0 40.0 25 34.124685761235924 37.0 33.0 40.0 15.0 40.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.351477120380121 1101 2 0.4805034351327109 1101 3 0.23536201317683236 1101 4 0.3882387632498876 1101 5 0.2019494887213682 1101 6 0.1945743031799978 1101 7 0.2229037199372499 1101 8 0.43556262248670663 1101 9 0.4739853891105028 1101 10 0.46083718948066377 1101 11 0.15007951319530122 1101 12 0.34400317036000416 1101 13 0.35138657795703665 1101 14 0.9487390890558842 1101 15 0.5831130521291215 1101 16 0.6681450287021562 1101 17 0.7267400260481054 1101 18 0.5810860510959799 1101 19 0.5895718718851768 1101 20 0.6949327917155799 1101 21 0.6549739722574373 1101 22 0.7765096371843541 1101 23 0.9870013104962183 1101 24 0.8656055694510414 1101 25 0.5676660060586514 1102 1 0.22342323439578138 1102 2 0.41008869574211104 1102 3 0.19516773733704085 1102 4 0.08909419196345425 1102 5 0.05847291913518404 1102 6 0.010254909820112346 1102 7 0.05523591166591757 1102 8 0.19781002609084197 1102 9 0.43155091783269484 1102 10 0.43544824105806157 1102 11 0.2144087222713864 1102 12 0.3804287882571984 1102 13 0.4148420173038474 1102 14 0.9540953202055817 1102 15 0.6633237373671008 1102 16 0.36362766191096085 1102 17 0.6314402820208258 1102 18 0.6197979507416349 1102 19 0.4729001023284809 1102 20 0.6609295103702735 1102 21 0.45466810609796227 1102 22 0.6722422986460757 1102 23 0.9868234440546146 1102 24 0.8114005209027866 1102 25 0.6376029172392421 1103 1 0.26469228753891727 1103 2 0.42516169414738414 1103 3 0.13954765742850483 1103 4 0.034671307197726264 1103 5 0.07664098314298684 1103 6 0.14845148197974822 1103 7 0.021532705898870574 1103 8 0.35735642492863917 1103 9 0.5174652143303575 1103 10 0.5432760594982327 1103 11 0.2440073904361597 1103 12 0.37578559828887137 1103 13 0.24797467880392077 1103 14 0.8821803982270211 1103 15 0.6286238111023366 1103 16 0.41189827560131675 1103 17 0.36134329041565394 1103 18 0.48412755629824034 1103 19 0.6329468332968986 1103 20 0.6761619025507386 1103 21 0.6812208216617748 1103 22 0.5466702393166187 1103 23 0.9645365134371175 1103 24 0.6843841896466358 1103 25 0.684001032015118 1104 1 0.21721623241425903 1104 2 0.48687688981222266 1104 3 0.17108797287444943 1104 4 0.2409052538844847 1104 5 0.1295756672205286 1104 6 0.1047571657197679 1104 7 0.21056849636728714 1104 8 0.3462942017711015 1104 9 0.6061138645910518 1104 10 0.5767728223515434 1104 11 0.38689884815772047 1104 12 0.4076895586799054 1104 13 0.5224103678485292 1104 14 0.9318317749495719 1104 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0.18914595493104258 2213 15 0.023844423465696707 2213 16 0.23390839277335118 2213 17 0.3128599616132348 2213 18 0.2932542368718103 2213 19 0.3463317414485445 2213 20 0.41179943350100956 2213 21 -0.25746296450520845 2213 22 -0.6322872773822468 2213 23 0.11082725223616308 2213 24 0.2770290725094853 2213 25 -0.3431712338929884 2214 1 -0.11611175211308478 2214 2 -0.32366109769549567 2214 3 -0.310919103883446 2214 4 -0.46948847690079276 2214 5 -0.27553639946723507 2214 6 -0.08924017414071983 2214 7 -0.32568479041647436 2214 8 -0.03727074636445593 2214 9 -0.010912374120522372 2214 10 -1.8607102757090956 2214 11 -0.8476041942776362 2214 12 -0.3794854193986126 2214 13 -0.1920581753633286 2214 14 -0.5803942727233604 2214 15 -0.45680309573199906 2214 16 -0.6249019882035398 2214 17 -0.34423136208520333 2214 18 -0.3382352767361283 2214 19 -1.1578836592376973 2214 20 -2.3500194917803086 2214 21 -1.7067028664310158 2214 22 -2.054219938599857 2214 23 -2.322081532113593 2214 24 -1.386012192564941 2214 25 -1.5242171572083478 2215 1 -0.19310977330924928 2215 2 -0.3234376400005061 2215 3 -0.7814611916246257 2215 4 -0.27478394297853725 2215 5 -0.03344645808878255 2215 6 -0.14898726128372175 2215 7 -0.12091847216046858 2215 8 -0.11033522456667555 2215 9 -0.23020000474014068 2215 10 -0.2533797632140278 2215 11 -0.3991280382450384 2215 12 -0.4057826085070815 2215 13 -0.2193873952415757 2215 14 -0.11029016206435927 2215 15 -0.5298380087622689 2215 16 -0.3374432591208958 2215 17 -0.1510264011868614 2215 18 -0.048699422433294615 2215 19 -2.059977560974069 2215 20 -2.278663605492085 2215 21 -2.9197680596978586 2215 22 -2.1733558930117383 2215 23 -3.374097670572109 2215 24 -2.24349775032222 2215 25 -1.5206095186680173 2216 1 -1.2414276019791224 2216 2 -1.0141142741827593 2216 3 -0.5825796426148138 2216 4 -0.6346811321001837 2216 5 -1.3841521765612015 2216 6 -1.2743795721766915 2216 7 -0.7389664292326117 2216 8 -4.746239154563991 2216 9 -3.548432236534971 2216 10 -1.7241250029228468 2216 11 -0.6520902244930369 2216 12 -0.7774648633327743 2216 13 -1.6436443612073859 2216 14 -3.05504348136116 2216 15 -1.51407482446389 2216 16 -2.554849357915643 2216 17 -2.249088775160736 2216 18 -2.2721613435828587 2216 19 -1.2882738756187706 2216 20 -0.6005907389082665 2216 21 -0.35169721092364625 2216 22 -0.36088204452001804 2216 23 -0.6768483207938587 2216 24 -0.2555299072493611 2216 25 0.18132126072138277 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 79.0 8 407.0 9 1113.0 10 2057.0 11 3764.0 12 6227.0 13 8796.0 14 11515.0 15 14248.0 16 16215.0 17 17296.0 18 17596.0 19 18473.0 20 18785.0 21 19870.0 22 21286.0 23 22128.0 24 23319.0 25 24615.0 26 26320.0 27 29461.0 28 33339.0 29 38624.0 30 46057.0 31 56814.0 32 75107.0 33 104413.0 34 148405.0 35 231646.0 36 394947.0 37 1198436.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.50998832952554 20.526197744215132 13.44875825391455 23.515055672344776 2 15.297056778925661 20.469861791717232 40.12791193103535 24.10516949832176 3 19.518692084338547 25.179854747834657 28.196534002211433 27.104919165615364 4 12.59143509191783 16.803911164089786 36.656824391408335 33.94782935258405 5 13.662864838135 37.7681706866059 34.36001835094626 14.208946124312838 6 32.55862476275318 37.152984958342614 16.791374059088803 13.497016219815402 7 27.324663795400017 32.365405100558306 21.815723358158277 18.4942077458834 8 24.985986307434764 37.55605001985669 19.84392071765307 17.614042955055474 9 24.393677171524622 17.883530684202885 21.308345437367986 36.414446706904506 10 15.012856479430011 30.847113923685033 32.591954420493146 21.54807517639181 11 32.89462183174973 25.84445503473133 21.822970940303467 19.437952193215477 12 21.46766621037086 27.606529615776637 30.481611228373133 20.444192945479365 13 27.087530398437583 23.467346785983977 25.833056854341745 23.612065961236695 14 20.733883242220234 23.412855402845313 27.27624085781226 28.577020497122195 15 23.864120913157326 30.285029129977083 23.142351148287354 22.708498808578238 16 21.989730555341254 30.880313578720187 25.623256328333397 21.50669953760516 17 21.23717507043094 30.093250449112436 27.54352314691478 21.12605133354185 18 21.530096012939552 29.36462874794019 28.43326827073338 20.67200696838687 19 21.63070031025923 28.78479015318384 28.81534483466269 20.769164701894237 20 22.581519239992367 28.31786974099148 28.10565863350336 20.994952385512793 21 22.413496050048813 28.461593756144758 27.946242174167534 21.178668019638888 22 21.815766444344632 28.118875669409654 28.108150662763578 21.957207223482136 23 21.67617537917267 28.473843085516037 28.19590293088425 21.65407860442704 24 21.757886028021563 28.564251323759727 28.014643539714314 21.663219108504403 25 22.281328243960814 27.79952940316164 27.189688307671915 22.72945404520563 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2524.0 1 2524.0 2 2818.5 3 3113.0 4 3113.0 5 3113.0 6 4200.0 7 5287.0 8 5287.0 9 5287.0 10 7652.5 11 10018.0 12 10018.0 13 10018.0 14 15882.5 15 21747.0 16 21747.0 17 21747.0 18 34185.5 19 46624.0 20 46624.0 21 46624.0 22 66284.5 23 85945.0 24 85945.0 25 85945.0 26 112598.5 27 139252.0 28 139252.0 29 139252.0 30 167431.5 31 195611.0 32 195611.0 33 195611.0 34 221145.0 35 246679.0 36 246679.0 37 246679.0 38 264980.5 39 283282.0 40 283282.0 41 283282.0 42 288968.0 43 294654.0 44 294654.0 45 294654.0 46 316132.0 47 337610.0 48 337610.0 49 337610.0 50 317880.0 51 298150.0 52 298150.0 53 298150.0 54 266596.0 55 235042.0 56 235042.0 57 235042.0 58 206350.0 59 177658.0 60 177658.0 61 177658.0 62 149218.0 63 120778.0 64 120778.0 65 120778.0 66 95355.0 67 69932.0 68 69932.0 69 69932.0 70 51903.0 71 33874.0 72 33874.0 73 33874.0 74 24469.5 75 15065.0 76 15065.0 77 15065.0 78 10250.5 79 5436.0 80 5436.0 81 5436.0 82 3742.5 83 2049.0 84 2049.0 85 2049.0 86 1372.5 87 696.0 88 696.0 89 696.0 90 473.5 91 251.0 92 251.0 93 251.0 94 154.0 95 57.0 96 57.0 97 57.0 98 41.0 99 25.0 100 25.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.029984506105020257 2 0.012541048180806952 3 0.01846954368446115 4 0.02017968661820755 5 0.016417372163965466 6 0.005054422448628256 7 0.0 8 1.5201270522190245E-4 9 1.9001588152737806E-4 10 3.800317630547561E-5 11 6.460539971930854E-4 12 9.500794076368903E-4 13 0.0021281778731066345 14 5.320444682766586E-4 15 0.0018621556389683048 16 0.0022041842257175853 17 0.0022041842257175853 18 0.001786149286357354 19 4.180349393602317E-4 20 6.460539971930854E-4 21 0.07927462577322213 22 0.07562632084789646 23 0.07661440343183884 24 0.0010640889365533172 25 0.2187842859906231 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2631359.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.03156237480052 #Duplication Level Percentage of deduplicated Percentage of total 1 89.72034980028297 70.01019071729702 2 7.4488364266370874 11.62488688489636 3 1.375329461813379 3.219573200561745 4 0.5199137852903964 1.6227873986562489 5 0.2500614099684312 0.97563412547411 6 0.143157583901356 0.670248596257464 7 0.10405515913846114 0.5683710648512837 8 0.07264590653665991 0.4534938869751444 9 0.05121635122787899 0.3596842714903137 >10 0.28246845706679835 4.078116373817421 >50 0.020939545598375707 1.1191934106023282 >100 0.008485283915196044 1.1729203394663632 >500 0.0011238280448519868 0.6711264400375773 >1k 0.0012704143115563524 2.2250651509698067 >5k 4.8862088906013554E-5 0.23822281543677565 >10k+ 9.772417781202711E-5 0.9904853232100765 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 13862 0.5268000299465029 No Hit CCCATGTACTCTGCGTTGATACCAC 12116 0.4604464841171425 No Hit GAGTACATGGGAAGCAGTGGTATCA 6248 0.2374438455566116 No Hit CATGTACTCTGCGTTGATACCACTG 4091 0.1554709942657007 No Hit GCGTTGATACCACTGCTTCCCATGT 4065 0.15448291168175834 No Hit GTATCAACGCAGAGTACATGGGAAG 3971 0.15091061310904366 No Hit GTATCAACGCAGAGTACTTTTTTTT 3870 0.1470722923021906 No Hit ACGCAGAGTACATGGGAAGCAGTGG 3839 0.14589419383672086 No Hit TATCAACGCAGAGTACATGGGAAGC 2934 0.11150131928026545 No Hit GGTATCAACGCAGAGTACATGGGAA 2855 0.10849906835213288 No Hit GCTTCCCATGTACTCTGCGTTGATA 2758 0.10481276025050172 No Hit GTGGTATCAACGCAGAGTACATGGG 2751 0.10454673801636341 No Hit GGTATCAACGCAGAGTACTTTTTTT 2727 0.103634661785032 No Hit ACTCTGCGTTGATACCACTGCTTCC 2659 0.10105044579625964 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 3.800317630547561E-5 0.0 6 0.0 0.0 0.0 3.800317630547561E-5 0.0 7 0.0 0.0 0.0 3.800317630547561E-5 0.0 8 0.0 0.0 0.0 3.800317630547561E-5 0.0 9 0.0 0.0 0.0 1.1400952891642684E-4 0.0 10 0.0 0.0 0.0 1.1400952891642684E-4 3.800317630547561E-5 11 0.0 0.0 0.0 1.5201270522190245E-4 3.800317630547561E-5 12 0.0 0.0 0.0 1.9001588152737806E-4 3.800317630547561E-5 13 0.0 0.0 0.0 1.9001588152737806E-4 3.800317630547561E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCTCGTC 70 0.0014962237 10.853461 12 AGGGTCG 85 6.610996E-4 10.056369 5 CGGGACT 105 4.1164574E-5 9.950331 4 AGACCGT 105 4.1250616E-5 9.94844 6 TTAGGAC 220 1.8189894E-12 9.92996 3 GAGACCG 90 0.0011165437 9.497682 5 TATCGGT 160 6.6747816E-8 9.4967785 13 ATCGGTC 150 2.6637645E-7 9.496599 14 TATACTG 580 0.0 9.333928 5 ACTATCG 145 1.7150633E-6 9.169304 11 GTCTCGC 135 6.8404224E-6 9.1492195 1 CCGTCCC 115 1.11540794E-4 9.083703 9 AGGACCG 105 4.5050596E-4 9.045411 5 GCACCGT 105 4.5119104E-4 9.044036 6 CTCGTCT 95 0.0018299702 8.996948 13 GTGTAGA 350 0.0 8.958192 1 AACACCG 85 0.0074525215 8.938994 5 ACTGATC 245 1.6370905E-11 8.9151745 8 GTATTAG 420 0.0 8.822461 1 TAGGACA 335 0.0 8.789233 4 >>END_MODULE